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The map label for this gene is ligD [H]

Identifier: 116248899

GI number: 116248899

Start: 236550

End: 239195

Strand: Reverse

Name: ligD [H]

Synonym: pRL120229

Alternate gene names: 116248899

Gene position: 239195-236550 (Counterclockwise)

Preceding gene: 116248901

Following gene: 116248898

Centisome position: 27.49

GC content: 61.56

Gene sequence:

>2646_bases
ATGGCCAGTGACAAACTATCGACCTATCGGTCGAAGCGCGACTTTAAAAAAACGGAGGAGCCGAGCGGTGAAAAACGCCT
GGCGCGCAGCAATCGCCGCCGCTTCGTCATCCAGAAACATGATGCCACCCGGCTGCACTACGACCTGCGGCTCGAGCTGG
ATGGCGTCTTCAAATCCTGGGCGGTCACCAAAGGCCCGTCTCTCGACCCGCACGACAAGCGGCTGGCAGTTGAGGTCGAG
GATCATCCGCTCGACTATGGCGACTTCGAAGGCACCATTCCGAAAGGTCAGTATGGCGGTGGGACGGTCATGCTCTGGGA
CCGGGGCTATTGGGAACCGGAAGGAAAGAAGAGCCCCGAGCAGGCCCTCGCCAAGGGTGACTTCAAGTTCACCTTGGAAG
GCAAACGCCTGCACGGCAGCTTCGTGCTGGTGCGCATGCGCAATGATCGCAACGGCGGCAACCGGACAAATTGGCTGCTC
ATCAAACACCATGATGAGTTCTCCGTCGAAAACAATGGCGAAGCGATCCTCGAGGAGAACGACACCTCCGTCGCCTCCGG
CCGAACCATGGAGATGATTGCCGCCGGCAAGGGACGAAAGCCCAAGCCCTTCATGGTCGAGGGTGGCGACGTTCAGGCTG
ATGCCGTCTGGGACAGCAATGACGGACTGGCTGCCGAAGAGCGAAAAGAGGGCGCCGGAGGCGGTCGGAAATCGAAACCG
GCCACAAACGTCGATCTGCCGGATTTCATTGCGCCGCAACTCTGTCAGACCCTGGAACGGCCTCCGGCCGGCACCGGCTG
GCTTCACGAGATCAAGTTCGACGGTTACCGGATCCAGATGCGAGTGCTGGATGGCGAGGCGACGCTGAAGACCAGGAAAG
GGCTCGACTGGACGGCCAAATATCTTGAGATCGCCGAGGCAGCGTCGACATTGCCCGATTCGATCATCGACGGCGAGATC
TGCGCCCTCGACGATCATGGCGTTCCTGATTTCGCGGCCCTGCAGGCGGCCCTTTCGGAAGGCAAGACCGGCGAGCTCGT
CTATTTCGCCTTCGATCTTCTCTATGAGGGAGGCGAGGACGTGCGATCTCTACCGCTCATCGAGCGCAAGGCGCGGCTTC
AAAGCCTTCTGTCCGACGCCGGTAGTGATCCGCGCATCCGTTTCGTCGAGCATTTCGACACCGGCGGTGATGCAGTTCTC
CGGTCTGCCTGCAAACTCTCACTGGAAGGCATCGTCTCGAAAGAGGCGGATGCACCCTATCAATCCGGCCGCACCGAAAG
CTGGGCGAAGTCGAAATGCCGCTCCGGCCACGAGGTGGTGATCGGCGCCTATGCCAAGACCAACGGGAAATTCCGATCCC
TGCTGGTCGGCGTCTATCACGGCGACCACTTCGTCTATGTCGGCCGCGTCGGCACGGGATATGGCGCGAAGAAAGTGGAA
ACGCTGCTGCCGAAGTTGAAAGCGCTCGAGACGGCGAAATCGCCGTTCACCGGCATCGGCGCGCCGAAAAAAGAGGCCGA
GGTCACCTGGCTGAAACCCGAGCTGGTGGCGGAAATCGAATTCGCGGGCTGGACGGCCGACGGCATTGTCCGGCAGGCGG
CCTTCAAGGGACTGCGGGACGACAAGCCGGCCAAAGAGGTCAAGGCCGATCGGCCGGCAAAGCCCGCGCAAACTGACGTG
CCGCAGCCGGTGGTGGAGGCGAAGGCCAGACCCATCCGCCGAAAAAGCGCCAAGGCCGAGGTCATGGGGGTACTGATATC
CAACCCGGACAAGCCGCTATGGCCGAATGCCAATGACGGAAAACCCGTTACCAAGGAGGAACTGGCCCGCTACTACGAAG
CCGTCGGCTCCTGGCTGATCGAGCACATCAAGGGACGGCCGTGTTCGATCATCCGCGCGCCCGACGGGATCGGCGGCGAG
CAGTTTTTCCAGCGCCATGCGATGCTCGGCACCTCGAACCTTCTCGAACTGGTCAAGGTCTTCGGCGATAAAAAGCCCTA
TCTGCAGATCGACCGGGTCGAAGGCTTGGCTGCCGTCGCGCAGATCGGCGCCGTCGAACTGCATCCCTGGAATTGCGGAC
CGCATCAGCCTGAGGTGCCGGGCCGGCTCGTCTTCGACCTTGATCCAGGTCCGGACGTACCATTCTGCGCGGTCGTTTCG
GCAGCCCGCGAAATGCGCGATCGTCTCGACGCGCTCGGCCTGATCGGCTTTTGCAAGACGACCGGCGGCAAGGGTCTGCA
CGTCGTCACTCCACTCGCAATCAACAAGCGCAAGCCGCTTTCGTGGGCCGAAGCGAAGAGCTTCGCACACGACGTCTGCC
AGCAGATGGCGCGCGACAATCCCGATCTCTACCTGATCAAGATGACGAAGAGCCTGAGGAACGGCCGGATCTTCCTCGAC
TATCTGCGTAACGACCGGATGGCGACGGCAGTGGCGCCTTTGTCACCCCGCGCCCGGCCGGGTGCCACCGTCTCGATGCC
GCTGACCTGGACCCAGGTCAAATCAGATCTGGACCCCAAGCGCTTCACCATCCGCACCGTTCCGGCCTTGCTGTCAAAAT
CTTCCGCCTGGGAAGATTACAATGACGGCAAGCGTCCGCTGGAACAGGCGATCAAGCGGCTCGGCAAGGGCTTCAAGGCG
GCTTGA

Upstream 100 bases:

>100_bases
ATCCCAGCCGTTATATCCGCTGACGGCTGGGGGAGCACCCTGATGGAACTTCCAGCCGCCAGGCGGCATTTCGCTGTCGG
GGTTGCTTTCGGGAACGACC

Downstream 100 bases:

>100_bases
GCATCCGAGTGAATTTCTCTGCATGCCGATATTGCTCTTCACGAAATTGCTCCAAATAACTGTGGAATCGATCGAGGACA
TCCAAGCGTTGACAATCTCT

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 881; Mature: 880

Protein sequence:

>881_residues
MASDKLSTYRSKRDFKKTEEPSGEKRLARSNRRRFVIQKHDATRLHYDLRLELDGVFKSWAVTKGPSLDPHDKRLAVEVE
DHPLDYGDFEGTIPKGQYGGGTVMLWDRGYWEPEGKKSPEQALAKGDFKFTLEGKRLHGSFVLVRMRNDRNGGNRTNWLL
IKHHDEFSVENNGEAILEENDTSVASGRTMEMIAAGKGRKPKPFMVEGGDVQADAVWDSNDGLAAEERKEGAGGGRKSKP
ATNVDLPDFIAPQLCQTLERPPAGTGWLHEIKFDGYRIQMRVLDGEATLKTRKGLDWTAKYLEIAEAASTLPDSIIDGEI
CALDDHGVPDFAALQAALSEGKTGELVYFAFDLLYEGGEDVRSLPLIERKARLQSLLSDAGSDPRIRFVEHFDTGGDAVL
RSACKLSLEGIVSKEADAPYQSGRTESWAKSKCRSGHEVVIGAYAKTNGKFRSLLVGVYHGDHFVYVGRVGTGYGAKKVE
TLLPKLKALETAKSPFTGIGAPKKEAEVTWLKPELVAEIEFAGWTADGIVRQAAFKGLRDDKPAKEVKADRPAKPAQTDV
PQPVVEAKARPIRRKSAKAEVMGVLISNPDKPLWPNANDGKPVTKEELARYYEAVGSWLIEHIKGRPCSIIRAPDGIGGE
QFFQRHAMLGTSNLLELVKVFGDKKPYLQIDRVEGLAAVAQIGAVELHPWNCGPHQPEVPGRLVFDLDPGPDVPFCAVVS
AAREMRDRLDALGLIGFCKTTGGKGLHVVTPLAINKRKPLSWAEAKSFAHDVCQQMARDNPDLYLIKMTKSLRNGRIFLD
YLRNDRMATAVAPLSPRARPGATVSMPLTWTQVKSDLDPKRFTIRTVPALLSKSSAWEDYNDGKRPLEQAIKRLGKGFKA
A

Sequences:

>Translated_881_residues
MASDKLSTYRSKRDFKKTEEPSGEKRLARSNRRRFVIQKHDATRLHYDLRLELDGVFKSWAVTKGPSLDPHDKRLAVEVE
DHPLDYGDFEGTIPKGQYGGGTVMLWDRGYWEPEGKKSPEQALAKGDFKFTLEGKRLHGSFVLVRMRNDRNGGNRTNWLL
IKHHDEFSVENNGEAILEENDTSVASGRTMEMIAAGKGRKPKPFMVEGGDVQADAVWDSNDGLAAEERKEGAGGGRKSKP
ATNVDLPDFIAPQLCQTLERPPAGTGWLHEIKFDGYRIQMRVLDGEATLKTRKGLDWTAKYLEIAEAASTLPDSIIDGEI
CALDDHGVPDFAALQAALSEGKTGELVYFAFDLLYEGGEDVRSLPLIERKARLQSLLSDAGSDPRIRFVEHFDTGGDAVL
RSACKLSLEGIVSKEADAPYQSGRTESWAKSKCRSGHEVVIGAYAKTNGKFRSLLVGVYHGDHFVYVGRVGTGYGAKKVE
TLLPKLKALETAKSPFTGIGAPKKEAEVTWLKPELVAEIEFAGWTADGIVRQAAFKGLRDDKPAKEVKADRPAKPAQTDV
PQPVVEAKARPIRRKSAKAEVMGVLISNPDKPLWPNANDGKPVTKEELARYYEAVGSWLIEHIKGRPCSIIRAPDGIGGE
QFFQRHAMLGTSNLLELVKVFGDKKPYLQIDRVEGLAAVAQIGAVELHPWNCGPHQPEVPGRLVFDLDPGPDVPFCAVVS
AAREMRDRLDALGLIGFCKTTGGKGLHVVTPLAINKRKPLSWAEAKSFAHDVCQQMARDNPDLYLIKMTKSLRNGRIFLD
YLRNDRMATAVAPLSPRARPGATVSMPLTWTQVKSDLDPKRFTIRTVPALLSKSSAWEDYNDGKRPLEQAIKRLGKGFKA
A
>Mature_880_residues
ASDKLSTYRSKRDFKKTEEPSGEKRLARSNRRRFVIQKHDATRLHYDLRLELDGVFKSWAVTKGPSLDPHDKRLAVEVED
HPLDYGDFEGTIPKGQYGGGTVMLWDRGYWEPEGKKSPEQALAKGDFKFTLEGKRLHGSFVLVRMRNDRNGGNRTNWLLI
KHHDEFSVENNGEAILEENDTSVASGRTMEMIAAGKGRKPKPFMVEGGDVQADAVWDSNDGLAAEERKEGAGGGRKSKPA
TNVDLPDFIAPQLCQTLERPPAGTGWLHEIKFDGYRIQMRVLDGEATLKTRKGLDWTAKYLEIAEAASTLPDSIIDGEIC
ALDDHGVPDFAALQAALSEGKTGELVYFAFDLLYEGGEDVRSLPLIERKARLQSLLSDAGSDPRIRFVEHFDTGGDAVLR
SACKLSLEGIVSKEADAPYQSGRTESWAKSKCRSGHEVVIGAYAKTNGKFRSLLVGVYHGDHFVYVGRVGTGYGAKKVET
LLPKLKALETAKSPFTGIGAPKKEAEVTWLKPELVAEIEFAGWTADGIVRQAAFKGLRDDKPAKEVKADRPAKPAQTDVP
QPVVEAKARPIRRKSAKAEVMGVLISNPDKPLWPNANDGKPVTKEELARYYEAVGSWLIEHIKGRPCSIIRAPDGIGGEQ
FFQRHAMLGTSNLLELVKVFGDKKPYLQIDRVEGLAAVAQIGAVELHPWNCGPHQPEVPGRLVFDLDPGPDVPFCAVVSA
AREMRDRLDALGLIGFCKTTGGKGLHVVTPLAINKRKPLSWAEAKSFAHDVCQQMARDNPDLYLIKMTKSLRNGRIFLDY
LRNDRMATAVAPLSPRARPGATVSMPLTWTQVKSDLDPKRFTIRTVPALLSKSSAWEDYNDGKRPLEQAIKRLGKGFKAA

Specific function: Probably involved in the repair of DNA double-strand breaks by non-homologous-end joining (NHEJ) during spore germination [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR014146
- InterPro:   IPR014145
- InterPro:   IPR014143 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 97242; Mature: 97111

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDKLSTYRSKRDFKKTEEPSGEKRLARSNRRRFVIQKHDATRLHYDLRLELDGVFKSW
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEECCCEEEEEEEEEEECCHHHHH
AVTKGPSLDPHDKRLAVEVEDHPLDYGDFEGTIPKGQYGGGTVMLWDRGYWEPEGKKSPE
EECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHH
QALAKGDFKFTLEGKRLHGSFVLVRMRNDRNGGNRTNWLLIKHHDEFSVENNGEAILEEN
HHHHCCCEEEEEECCEEECEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCEEEECC
DTSVASGRTMEMIAAGKGRKPKPFMVEGGDVQADAVWDSNDGLAAEERKEGAGGGRKSKP
CCCCCCCCEEEEEECCCCCCCCCEEEECCCEEEEEEECCCCCCCCHHHHCCCCCCCCCCC
ATNVDLPDFIAPQLCQTLERPPAGTGWLHEIKFDGYRIQMRVLDGEATLKTRKGLDWTAK
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEEEEEECCCEEEHHCCCCCHHHH
YLEIAEAASTLPDSIIDGEICALDDHGVPDFAALQAALSEGKTGELVYFAFDLLYEGGED
HHHHHHHHHHCCCHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEEHHHCCCCH
VRSLPLIERKARLQSLLSDAGSDPRIRFVEHFDTGGDAVLRSACKLSLEGIVSKEADAPY
HHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCC
QSGRTESWAKSKCRSGHEVVIGAYAKTNGKFRSLLVGVYHGDHFVYVGRVGTGYGAKKVE
CCCCCCHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEEECCEEEEEECCCCCCCHHHHH
TLLPKLKALETAKSPFTGIGAPKKEAEVTWLKPELVAEIEFAGWTADGIVRQAAFKGLRD
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHEEEEEECCCCHHHHHHHHHHCCCCC
DKPAKEVKADRPAKPAQTDVPQPVVEAKARPIRRKSAKAEVMGVLISNPDKPLWPNANDG
CCCHHHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHCCCCEEEEEEECCCCCCCCCCCCCC
KPVTKEELARYYEAVGSWLIEHIKGRPCSIIRAPDGIGGEQFFQRHAMLGTSNLLELVKV
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHH
FGDKKPYLQIDRVEGLAAVAQIGAVELHPWNCGPHQPEVPGRLVFDLDPGPDVPFCAVVS
HCCCCCCEEEEHHHHHHHHHHHCCEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHH
AAREMRDRLDALGLIGFCKTTGGKGLHVVTPLAINKRKPLSWAEAKSFAHDVCQQMARDN
HHHHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
PDLYLIKMTKSLRNGRIFLDYLRNDRMATAVAPLSPRARPGATVSMPLTWTQVKSDLDPK
CCEEEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCEEEECEEHHHHHCCCCCC
RFTIRTVPALLSKSSAWEDYNDGKRPLEQAIKRLGKGFKAA
EEEEEHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ASDKLSTYRSKRDFKKTEEPSGEKRLARSNRRRFVIQKHDATRLHYDLRLELDGVFKSW
CCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEECCCEEEEEEEEEEECCHHHHH
AVTKGPSLDPHDKRLAVEVEDHPLDYGDFEGTIPKGQYGGGTVMLWDRGYWEPEGKKSPE
EECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHH
QALAKGDFKFTLEGKRLHGSFVLVRMRNDRNGGNRTNWLLIKHHDEFSVENNGEAILEEN
HHHHCCCEEEEEECCEEECEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCEEEECC
DTSVASGRTMEMIAAGKGRKPKPFMVEGGDVQADAVWDSNDGLAAEERKEGAGGGRKSKP
CCCCCCCCEEEEEECCCCCCCCCEEEECCCEEEEEEECCCCCCCCHHHHCCCCCCCCCCC
ATNVDLPDFIAPQLCQTLERPPAGTGWLHEIKFDGYRIQMRVLDGEATLKTRKGLDWTAK
CCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEEEEEECCCEEEHHCCCCCHHHH
YLEIAEAASTLPDSIIDGEICALDDHGVPDFAALQAALSEGKTGELVYFAFDLLYEGGED
HHHHHHHHHHCCCHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEEHHHCCCCH
VRSLPLIERKARLQSLLSDAGSDPRIRFVEHFDTGGDAVLRSACKLSLEGIVSKEADAPY
HHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCC
QSGRTESWAKSKCRSGHEVVIGAYAKTNGKFRSLLVGVYHGDHFVYVGRVGTGYGAKKVE
CCCCCCHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEEECCEEEEEECCCCCCCHHHHH
TLLPKLKALETAKSPFTGIGAPKKEAEVTWLKPELVAEIEFAGWTADGIVRQAAFKGLRD
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHEEEEEECCCCHHHHHHHHHHCCCCC
DKPAKEVKADRPAKPAQTDVPQPVVEAKARPIRRKSAKAEVMGVLISNPDKPLWPNANDG
CCCHHHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHCCCCEEEEEEECCCCCCCCCCCCCC
KPVTKEELARYYEAVGSWLIEHIKGRPCSIIRAPDGIGGEQFFQRHAMLGTSNLLELVKV
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHH
FGDKKPYLQIDRVEGLAAVAQIGAVELHPWNCGPHQPEVPGRLVFDLDPGPDVPFCAVVS
HCCCCCCEEEEHHHHHHHHHHHCCEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHH
AAREMRDRLDALGLIGFCKTTGGKGLHVVTPLAINKRKPLSWAEAKSFAHDVCQQMARDN
HHHHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
PDLYLIKMTKSLRNGRIFLDYLRNDRMATAVAPLSPRARPGATVSMPLTWTQVKSDLDPK
CCEEEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCEEEECEEHHHHHCCCCCC
RFTIRTVPALLSKSSAWEDYNDGKRPLEQAIKRLGKGFKAA
EEEEEHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]