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The map label for this gene is rutF [H]

Identifier: 116248888

GI number: 116248888

Start: 226289

End: 226828

Strand: Reverse

Name: rutF [H]

Synonym: pRL120218

Alternate gene names: 116248888

Gene position: 226828-226289 (Counterclockwise)

Preceding gene: 116248893

Following gene: 116248887

Centisome position: 26.07

GC content: 59.81

Gene sequence:

>540_bases
ATGTCTGCCACTGCTGCCCTCGGCATAGCGCCGGCCCCCGCACCCATCAGCGATGCGTTTCGATCGACCATGCGCCGTTT
CCCGGCGACGGTGACAGTCATTTCTGCTTGTCGCAACGGCGCGGATCATGGAATGACGGCCACCGCCGTCACATCACTTT
CGATGGATCCGCCATCTCTCATCATCTGCCTGAACAATCGCACCTACCTGCATGACATGCTGCTTGAGGTGCCGGAATTC
GCAGTCAGCATTCTGACCGACAGGCAGGCAGCCGTATCGGAAGGTTTCAGCGGCAAGATTGCGCCTGAGCGTCGGTTCGA
CGCTGCCGATTGGGTTCGCCATGAGCGCGGCATGATGGTGCTGGACACCGCTCATGCCTCGGTTGTCTGCCGCCGCATGG
GCGCAGTTCCCTATGGAACGCACACCATCTTCATCGGGCAGGTGGTGGATACACGCCATTCCGACGACACCATCGCGCTG
ATGTACGAGAATTCGAAATATTGCGCGCCACAGCACGCGCTTCCTGCATCCCAGAACTGA

Upstream 100 bases:

>100_bases
GGCTGAGTAAGGCAAGCATTTTCCCGCCAATTTATAAACTTGCATTGACAATAGTTGCAAATGCAATAATCTAGATGCCA
AGGAGACACGGGAGGAAATC

Downstream 100 bases:

>100_bases
AGGTAGTTCCATGAACAATACAGCCGCCCTGTTTCCCGCCTCGGCGCGGGTCAAGACCGCATGTCTTGCAGATCGCATCG
CCCCGCTGCTGGACGAAATC

Product: putative flavin reductase

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MSATAALGIAPAPAPISDAFRSTMRRFPATVTVISACRNGADHGMTATAVTSLSMDPPSLIICLNNRTYLHDMLLEVPEF
AVSILTDRQAAVSEGFSGKIAPERRFDAADWVRHERGMMVLDTAHASVVCRRMGAVPYGTHTIFIGQVVDTRHSDDTIAL
MYENSKYCAPQHALPASQN

Sequences:

>Translated_179_residues
MSATAALGIAPAPAPISDAFRSTMRRFPATVTVISACRNGADHGMTATAVTSLSMDPPSLIICLNNRTYLHDMLLEVPEF
AVSILTDRQAAVSEGFSGKIAPERRFDAADWVRHERGMMVLDTAHASVVCRRMGAVPYGTHTIFIGQVVDTRHSDDTIAL
MYENSKYCAPQHALPASQN
>Mature_178_residues
SATAALGIAPAPAPISDAFRSTMRRFPATVTVISACRNGADHGMTATAVTSLSMDPPSLIICLNNRTYLHDMLLEVPEFA
VSILTDRQAAVSEGFSGKIAPERRFDAADWVRHERGMMVLDTAHASVVCRRMGAVPYGTHTIFIGQVVDTRHSDDTIALM
YENSKYCAPQHALPASQN

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=155, Percent_Identity=30.9677419354839, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 19362; Mature: 19231

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATAALGIAPAPAPISDAFRSTMRRFPATVTVISACRNGADHGMTATAVTSLSMDPPSL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEHEEECCCCCCEE
IICLNNRTYLHDMLLEVPEFAVSILTDRQAAVSEGFSGKIAPERRFDAADWVRHERGMMV
EEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHCCEEE
LDTAHASVVCRRMGAVPYGTHTIFIGQVVDTRHSDDTIALMYENSKYCAPQHALPASQN
EECHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SATAALGIAPAPAPISDAFRSTMRRFPATVTVISACRNGADHGMTATAVTSLSMDPPSL
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEHEEECCCCCCEE
IICLNNRTYLHDMLLEVPEFAVSILTDRQAAVSEGFSGKIAPERRFDAADWVRHERGMMV
EEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHCCEEE
LDTAHASVVCRRMGAVPYGTHTIFIGQVVDTRHSDDTIALMYENSKYCAPQHALPASQN
EECHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA