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The map label for this gene is potB [H]

Identifier: 116248841

GI number: 116248841

Start: 170669

End: 171526

Strand: Reverse

Name: potB [H]

Synonym: pRL120170

Alternate gene names: 116248841

Gene position: 171526-170669 (Counterclockwise)

Preceding gene: 116248842

Following gene: 116248840

Centisome position: 19.72

GC content: 60.72

Gene sequence:

>858_bases
ATGAACGCAGGCGCCAAAAAACGGTTCGGGCCGCTGCTGTTGATCGCGCCCGCCGCGATCCTGCTCGGCTTCTTTCTCGT
GCTGCCGTATCTCAATATCATCGTGATGAGCTTTCGCGTGCCTGGGCAGGGCACGCCCTATGCGCCGGGCTTTACCACCG
GCAATTACAGCAAGTTCTTCTCGGACATCTTCTACATCAGCCAGATGGTCAACACGCTGTGGATCGGCTTCGTCACCACG
TTTGCCTGTCTGATTCTAGGCTATCCGGTGGCCTGGCAGCTGGCGCGGGGTACATCTGCGATCCGCTCCCTCGGCTACGG
ACTGGTGCTCTCACCACTTCTGGTCGGCATCGTGATTCGCAGCTACGGCTGGACGATCCTGCTCGGCAATAACGGCCTGA
TCAACCGGGCGCTGGCTGGCTGGGGCGTGATCGATGGGCCGCTGCCGCTGATGTACAATGCCCTCGGCATCGTCATTGCG
CTGGTGCATGTATTCCTGCCCTTCATGATCCTGCCGATAATGAGCGCGATCCAGGGCATCGATCCCTCGCTGGAATCGGC
CGCTCGTTCGCTCGGTGCCTCCAAGTTCAAGGCCTTCCGGCGCATCACACTGCCGTTGTCGCTGCCCGGCATTCAGGCCG
GCTGCATCCTGGTGTTCGTGCTGTCGCTAAGTGCCTATGTGACGCCTTCGCTGATCGGCGGCCTTCGGGTCAAAACCATG
GCGGTGACGGTGGTCGATGCCCTGATCGACACGTTCCAATGGCCGTTCGGTTCCGCGCTGGCGCTGATGTTGTCGGTCAC
AGGCGCGATCGTGGTGGTCATCTTCGGGCGGCTGACCGTCATGAAATGGAAGGCATAA

Upstream 100 bases:

>100_bases
TCGTCAACGTCCAGACGTTTCCGCGGATACGATTCGCCGATGGCGAGGAGGTGCTGTTGCGTGCACCTTCAGACCGCTTC
TGGCCGCTTGGATCCGCGGC

Downstream 100 bases:

>100_bases
AAGACATGTCGAAACCCTTCCTCACGGTGTACTGCCTGGTCATCTACGCCTTTCTCCTGGCGCCGATCTTGGTCGTCGTC
GGGGCGTCGTTCAATGCCGG

Product: putative permease component of ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNAGAKKRFGPLLLIAPAAILLGFFLVLPYLNIIVMSFRVPGQGTPYAPGFTTGNYSKFFSDIFYISQMVNTLWIGFVTT
FACLILGYPVAWQLARGTSAIRSLGYGLVLSPLLVGIVIRSYGWTILLGNNGLINRALAGWGVIDGPLPLMYNALGIVIA
LVHVFLPFMILPIMSAIQGIDPSLESAARSLGASKFKAFRRITLPLSLPGIQAGCILVFVLSLSAYVTPSLIGGLRVKTM
AVTVVDALIDTFQWPFGSALALMLSVTGAIVVVIFGRLTVMKWKA

Sequences:

>Translated_285_residues
MNAGAKKRFGPLLLIAPAAILLGFFLVLPYLNIIVMSFRVPGQGTPYAPGFTTGNYSKFFSDIFYISQMVNTLWIGFVTT
FACLILGYPVAWQLARGTSAIRSLGYGLVLSPLLVGIVIRSYGWTILLGNNGLINRALAGWGVIDGPLPLMYNALGIVIA
LVHVFLPFMILPIMSAIQGIDPSLESAARSLGASKFKAFRRITLPLSLPGIQAGCILVFVLSLSAYVTPSLIGGLRVKTM
AVTVVDALIDTFQWPFGSALALMLSVTGAIVVVIFGRLTVMKWKA
>Mature_285_residues
MNAGAKKRFGPLLLIAPAAILLGFFLVLPYLNIIVMSFRVPGQGTPYAPGFTTGNYSKFFSDIFYISQMVNTLWIGFVTT
FACLILGYPVAWQLARGTSAIRSLGYGLVLSPLLVGIVIRSYGWTILLGNNGLINRALAGWGVIDGPLPLMYNALGIVIA
LVHVFLPFMILPIMSAIQGIDPSLESAARSLGASKFKAFRRITLPLSLPGIQAGCILVFVLSLSAYVTPSLIGGLRVKTM
AVTVVDALIDTFQWPFGSALALMLSVTGAIVVVIFGRLTVMKWKA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=256, Percent_Identity=35.15625, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787080, Length=211, Percent_Identity=35.5450236966825, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1787713, Length=197, Percent_Identity=32.994923857868, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786980, Length=159, Percent_Identity=32.0754716981132, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787368, Length=69, Percent_Identity=40.5797101449275, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30589; Mature: 30589

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAGAKKRFGPLLLIAPAAILLGFFLVLPYLNIIVMSFRVPGQGTPYAPGFTTGNYSKFF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHH
SDIFYISQMVNTLWIGFVTTFACLILGYPVAWQLARGTSAIRSLGYGLVLSPLLVGIVIR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
SYGWTILLGNNGLINRALAGWGVIDGPLPLMYNALGIVIALVHVFLPFMILPIMSAIQGI
HCCCEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPSLESAARSLGASKFKAFRRITLPLSLPGIQAGCILVFVLSLSAYVTPSLIGGLRVKTM
CCCHHHHHHHHCHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AVTVVDALIDTFQWPFGSALALMLSVTGAIVVVIFGRLTVMKWKA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MNAGAKKRFGPLLLIAPAAILLGFFLVLPYLNIIVMSFRVPGQGTPYAPGFTTGNYSKFF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHH
SDIFYISQMVNTLWIGFVTTFACLILGYPVAWQLARGTSAIRSLGYGLVLSPLLVGIVIR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
SYGWTILLGNNGLINRALAGWGVIDGPLPLMYNALGIVIALVHVFLPFMILPIMSAIQGI
HCCCEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPSLESAARSLGASKFKAFRRITLPLSLPGIQAGCILVFVLSLSAYVTPSLIGGLRVKTM
CCCHHHHHHHHCHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AVTVVDALIDTFQWPFGSALALMLSVTGAIVVVIFGRLTVMKWKA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]