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The map label for this gene is kipI [H]

Identifier: 116248825

GI number: 116248825

Start: 151166

End: 152041

Strand: Reverse

Name: kipI [H]

Synonym: pRL120151

Alternate gene names: 116248825

Gene position: 152041-151166 (Counterclockwise)

Preceding gene: 116248826

Following gene: 116248824

Centisome position: 17.48

GC content: 59.82

Gene sequence:

>876_bases
ATGCCTGCGCGATATACCTTCGGAGGTGATGAACACCTCTTCGTCGAATGCAGCAGCGAGATGTCGCTGGAAGCCTTCTT
CAAGAGCTTGTCCATGGCGACAGCGGTCCGCGAAAGCCGGATCAGGGGCGTCACCGAGATCTGCCCGGCCAATGCCTCGT
TCCAGGTAAAGTTCAATCCCGATCTCATCAAGCCGGACGACATCCTGCGGGAGGTGCAGGCGATCGAGGGCGTGGCGGAA
AAATCCGAGCCGGTCCTGAAGACCCGCATCATCGAGATTCCGGTCTTCTATAATGATCCATGGACATACGAGACGCTGAT
GCGCTTTCGCGAACGGCACCAGGAACCCGGCGGAACGGATCTCGATTATGCTGCCCGGATCAACGGCTACGGCGGTGTCG
AGGATTTCGTCGCCGCCCATGCGGGTTCGCCCTGGTTCGTGTCGATGGTCGGTTTCGTGGCGGGGCTTCCGTTCATGTAC
CAGATGGTCGAGCGGCAGCGGCAGATCGAAGTGCCGAAATATCTGAGGCCACGCACCGACACGCCCAAACTGACGGTCGG
CCATGGCGGCTGCTTCGGCTGCATCTATTCGGTGCGCGGCGCCGGCGGCTACCAGATGTTCGGCGTCACGCCGATGCCGA
TCTTCGACCCGACGCAGCAAACGAGTTACCTGCGCGATTTCATGGTGTTCTTCCGTCCTGGTGATATCGTCAAGTTCAAG
CCGGTCGATCGCGACGGTTACGACACGGCAGTCGAAGAGGTCGACAAGAAGCTCTTCAGCCCGCCGATCCGCGAGGTCCG
GTTCGATCTTCGCGAATTCAGCAAGGATATCCACGGTTACAACGCCGCGCTGGAGGGCGTTCTCCATGGCCATTAA

Upstream 100 bases:

>100_bases
ATGCAAGCGTCGCCAAGGGCGCTTTCCATACCCGCTTTCTCGAACAATGGCTGGAGCACGATTTTCCCGCTCTGGCCGGC
CGGACCCAGGAGGTTGCCTG

Downstream 100 bases:

>100_bases
GGTGCTGCACCCGGGACTATCGACCAGCGTCCAGGATCTCGGCCGTCCGGGCTATTTCCACCTCGGCATCCCGATCGGCG
GCGCCATGGACCGCTACGCC

Product: putative urea amidohydrolyase homologue

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MPARYTFGGDEHLFVECSSEMSLEAFFKSLSMATAVRESRIRGVTEICPANASFQVKFNPDLIKPDDILREVQAIEGVAE
KSEPVLKTRIIEIPVFYNDPWTYETLMRFRERHQEPGGTDLDYAARINGYGGVEDFVAAHAGSPWFVSMVGFVAGLPFMY
QMVERQRQIEVPKYLRPRTDTPKLTVGHGGCFGCIYSVRGAGGYQMFGVTPMPIFDPTQQTSYLRDFMVFFRPGDIVKFK
PVDRDGYDTAVEEVDKKLFSPPIREVRFDLREFSKDIHGYNAALEGVLHGH

Sequences:

>Translated_291_residues
MPARYTFGGDEHLFVECSSEMSLEAFFKSLSMATAVRESRIRGVTEICPANASFQVKFNPDLIKPDDILREVQAIEGVAE
KSEPVLKTRIIEIPVFYNDPWTYETLMRFRERHQEPGGTDLDYAARINGYGGVEDFVAAHAGSPWFVSMVGFVAGLPFMY
QMVERQRQIEVPKYLRPRTDTPKLTVGHGGCFGCIYSVRGAGGYQMFGVTPMPIFDPTQQTSYLRDFMVFFRPGDIVKFK
PVDRDGYDTAVEEVDKKLFSPPIREVRFDLREFSKDIHGYNAALEGVLHGH
>Mature_290_residues
PARYTFGGDEHLFVECSSEMSLEAFFKSLSMATAVRESRIRGVTEICPANASFQVKFNPDLIKPDDILREVQAIEGVAEK
SEPVLKTRIIEIPVFYNDPWTYETLMRFRERHQEPGGTDLDYAARINGYGGVEDFVAAHAGSPWFVSMVGFVAGLPFMYQ
MVERQRQIEVPKYLRPRTDTPKLTVGHGGCFGCIYSVRGAGGYQMFGVTPMPIFDPTQQTSYLRDFMVFFRPGDIVKFKP
VDRDGYDTAVEEVDKKLFSPPIREVRFDLREFSKDIHGYNAALEGVLHGH

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=240, Percent_Identity=28.3333333333333, Blast_Score=72, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=292, Percent_Identity=23.972602739726, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 32994; Mature: 32863

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARYTFGGDEHLFVECSSEMSLEAFFKSLSMATAVRESRIRGVTEICPANASFQVKFNP
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEECC
DLIKPDDILREVQAIEGVAEKSEPVLKTRIIEIPVFYNDPWTYETLMRFRERHQEPGGTD
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
LDYAARINGYGGVEDFVAAHAGSPWFVSMVGFVAGLPFMYQMVERQRQIEVPKYLRPRTD
CCHHEEECCCCCHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHCCCCC
TPKLTVGHGGCFGCIYSVRGAGGYQMFGVTPMPIFDPTQQTSYLRDFMVFFRPGDIVKFK
CCEEEECCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
PVDRDGYDTAVEEVDKKLFSPPIREVRFDLREFSKDIHGYNAALEGVLHGH
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
PARYTFGGDEHLFVECSSEMSLEAFFKSLSMATAVRESRIRGVTEICPANASFQVKFNP
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEECC
DLIKPDDILREVQAIEGVAEKSEPVLKTRIIEIPVFYNDPWTYETLMRFRERHQEPGGTD
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
LDYAARINGYGGVEDFVAAHAGSPWFVSMVGFVAGLPFMYQMVERQRQIEVPKYLRPRTD
CCHHEEECCCCCHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHCCCCC
TPKLTVGHGGCFGCIYSVRGAGGYQMFGVTPMPIFDPTQQTSYLRDFMVFFRPGDIVKFK
CCEEEECCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
PVDRDGYDTAVEEVDKKLFSPPIREVRFDLREFSKDIHGYNAALEGVLHGH
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]