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The map label for this gene is purR [H]

Identifier: 116248789

GI number: 116248789

Start: 111412

End: 112446

Strand: Direct

Name: purR [H]

Synonym: pRL120115

Alternate gene names: 116248789

Gene position: 111412-112446 (Clockwise)

Preceding gene: 116248788

Following gene: 116248790

Centisome position: 12.81

GC content: 62.61

Gene sequence:

>1035_bases
ATGCGACAGACGGACCGGACGGCCGGAAAGAGACGCGGAGCCACGATTATCGACGTTGCCAGGGTGGCGGACGTCGCTGT
CGGCACCGTGTCGCGCTATCTCAACGGCGAGACGATCCGCCGCTCGAACCGCGAACAGATCGAGCGCGCAATCCAGGATC
TGGGCTACCGGCGCAATGCCGCAGCGGCCTCGATCCGTACCGACCTTACCCATATGATCGGCTTCCTGGTGCCGACCTTC
GACGAATTCCATGCCCGGATGTTGGAATATCTGGCCATTTCCGTGCGTCAGACCGGACGAGCGCTCTTGACCTACTGTCA
TGGCGGCGATCCGCGCGTGGTCGCCGAGGCTCTCGATTTCTTTGCCGCGCAACGCGTCGATGCACTGGTCATGGACGGCA
CGGCTGAGGTCTATGATCGCGTCGACGACCTGATCCATCACGACATTCCGATCATCTTCTACAATAACGACGTGCGTGGG
CTCGCGGCGGACCGGGTGATGGTCGAAAATCACCGCGCGAGCTACCGCCTAATCAGCCATTTGATCGATCTCGGCCATAC
GCGCATCGGCATGCTGACGGGTGACCCGCGCAATTCGTCGGGAATCGAACGCCTGGCCGGATACGAGCAGGCGCTGCGCG
ACCGGGGCATCGTGGTGGATGCTTCCCTGGCAGTGCGCGGCAACTGGCGCATGGATGGCGGCTACGAGGCGACCAAGCGA
CTGATGTCGCTGGAAAGCCCACCCACAGCTATTTTCTCCGCCAATTACGGCATGGCGGTCGGGGCGCTCAGCTGGCTGAA
GGAGAACGGCCGTCACGTGCCCGACGATATCTCGCTGGCGAGCTTCGACGACGTGGCGATCTTCCGCCTCTATGAGGCCG
GCATTACGGCGGTCGCCCAGCCAGTCGCCAGCATTGCCGAGACCATCACCGACATTCTCGTGGAACGCCTGACGGAACCA
TCAGGAGGGGCGACCCGCAGCGTCGTTCTTGAATGCGACATCATTTTGCGGGGCTCGACGCGACGGATCACCTGA

Upstream 100 bases:

>100_bases
CGCCCGCAACGCTGGTGGGCGGCAGAGCGCTGGGCTATACGACGGAAAAGCGCTTCCCCTGGCGCTATTTCCCCTCAATG
AGTGCAGGAGCCCGCTCGAC

Downstream 100 bases:

>100_bases
CGCGTTCTGCCCGCCAAGCGGTTAAAGGGAGGAAGCTTTGGCCAGCGTAGAATTACACGATATCGACAAGTCCTATGGCA
GTTTCGAAGCAATCGAAGGG

Product: putative transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRQTDRTAGKRRGATIIDVARVADVAVGTVSRYLNGETIRRSNREQIERAIQDLGYRRNAAAASIRTDLTHMIGFLVPTF
DEFHARMLEYLAISVRQTGRALLTYCHGGDPRVVAEALDFFAAQRVDALVMDGTAEVYDRVDDLIHHDIPIIFYNNDVRG
LAADRVMVENHRASYRLISHLIDLGHTRIGMLTGDPRNSSGIERLAGYEQALRDRGIVVDASLAVRGNWRMDGGYEATKR
LMSLESPPTAIFSANYGMAVGALSWLKENGRHVPDDISLASFDDVAIFRLYEAGITAVAQPVASIAETITDILVERLTEP
SGGATRSVVLECDIILRGSTRRIT

Sequences:

>Translated_344_residues
MRQTDRTAGKRRGATIIDVARVADVAVGTVSRYLNGETIRRSNREQIERAIQDLGYRRNAAAASIRTDLTHMIGFLVPTF
DEFHARMLEYLAISVRQTGRALLTYCHGGDPRVVAEALDFFAAQRVDALVMDGTAEVYDRVDDLIHHDIPIIFYNNDVRG
LAADRVMVENHRASYRLISHLIDLGHTRIGMLTGDPRNSSGIERLAGYEQALRDRGIVVDASLAVRGNWRMDGGYEATKR
LMSLESPPTAIFSANYGMAVGALSWLKENGRHVPDDISLASFDDVAIFRLYEAGITAVAQPVASIAETITDILVERLTEP
SGGATRSVVLECDIILRGSTRRIT
>Mature_344_residues
MRQTDRTAGKRRGATIIDVARVADVAVGTVSRYLNGETIRRSNREQIERAIQDLGYRRNAAAASIRTDLTHMIGFLVPTF
DEFHARMLEYLAISVRQTGRALLTYCHGGDPRVVAEALDFFAAQRVDALVMDGTAEVYDRVDDLIHHDIPIIFYNNDVRG
LAADRVMVENHRASYRLISHLIDLGHTRIGMLTGDPRNSSGIERLAGYEQALRDRGIVVDASLAVRGNWRMDGGYEATKR
LMSLESPPTAIFSANYGMAVGALSWLKENGRHVPDDISLASFDDVAIFRLYEAGITAVAQPVASIAETITDILVERLTEP
SGGATRSVVLECDIILRGSTRRIT

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=338, Percent_Identity=27.810650887574, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1789068, Length=306, Percent_Identity=29.4117647058824, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790194, Length=317, Percent_Identity=28.391167192429, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=31.5960912052117, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1787948, Length=309, Percent_Identity=29.126213592233, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=30.1694915254237, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787906, Length=321, Percent_Identity=28.0373831775701, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1786540, Length=311, Percent_Identity=27.6527331189711, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786268, Length=327, Percent_Identity=23.2415902140673, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI48994940, Length=294, Percent_Identity=25.1700680272109, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787580, Length=285, Percent_Identity=24.2105263157895, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790689, Length=288, Percent_Identity=24.3055555555556, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789456, Length=289, Percent_Identity=25.9515570934256, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38000; Mature: 38000

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQTDRTAGKRRGATIIDVARVADVAVGTVSRYLNGETIRRSNREQIERAIQDLGYRRNA
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCCH
AAASIRTDLTHMIGFLVPTFDEFHARMLEYLAISVRQTGRALLTYCHGGDPRVVAEALDF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
FAAQRVDALVMDGTAEVYDRVDDLIHHDIPIIFYNNDVRGLAADRVMVENHRASYRLISH
HHHHHHHHHEECCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHH
LIDLGHTRIGMLTGDPRNSSGIERLAGYEQALRDRGIVVDASLAVRGNWRMDGGYEATKR
HHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCHHHHHH
LMSLESPPTAIFSANYGMAVGALSWLKENGRHVPDDISLASFDDVAIFRLYEAGITAVAQ
HHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
PVASIAETITDILVERLTEPSGGATRSVVLECDIILRGSTRRIT
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCCC
>Mature Secondary Structure
MRQTDRTAGKRRGATIIDVARVADVAVGTVSRYLNGETIRRSNREQIERAIQDLGYRRNA
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCCCH
AAASIRTDLTHMIGFLVPTFDEFHARMLEYLAISVRQTGRALLTYCHGGDPRVVAEALDF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
FAAQRVDALVMDGTAEVYDRVDDLIHHDIPIIFYNNDVRGLAADRVMVENHRASYRLISH
HHHHHHHHHEECCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHH
LIDLGHTRIGMLTGDPRNSSGIERLAGYEQALRDRGIVVDASLAVRGNWRMDGGYEATKR
HHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCHHHHHH
LMSLESPPTAIFSANYGMAVGALSWLKENGRHVPDDISLASFDDVAIFRLYEAGITAVAQ
HHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
PVASIAETITDILVERLTEPSGGATRSVVLECDIILRGSTRRIT
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA