| Definition | Rhizobium leguminosarum bv. viciae 3841 plasmid pRL12, complete sequence. |
|---|---|
| Accession | NC_008378 |
| Length | 870,021 |
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The map label for this gene is dkgB [H]
Identifier: 116248783
GI number: 116248783
Start: 102545
End: 103369
Strand: Direct
Name: dkgB [H]
Synonym: pRL120107
Alternate gene names: 116248783
Gene position: 102545-103369 (Clockwise)
Preceding gene: 116248782
Following gene: 116248784
Centisome position: 11.79
GC content: 61.58
Gene sequence:
>825_bases ATGACGAAAGAAGCAATGGTCGCTGGCATCCCGCAGATGGGGTACGGCACATGGAACCGCTCGGCCGATGAAGCCCATCA GGGCGTCATCTGGGCGCTCGAAGCCGGATGCCGCCACATCGACACCGCCCAGGGCTACGGCAACGAGCAGGATGTCGCCC GCGGCATCAAGGACGCCGGCGTGGCGCGCAACGAAATCTTCATCACCACGAAGGTGAAGCCCGACAATTATGGCCCTGGA GCCGTCATGCCGTCGGTCAGGGAAAGCCTGGAAAAGCTCCGCGTCGACCAGGTCGACCTGTTGCTCCTGCACTGGCCGTC GCCGCATAACAAATATCCGCTTGCCGATTATCTCGGCCAGTTCGCCGAGGTTTTCGATTCCGGCCTTGCCAGGCAGATTG GCGTCTCGAACTTTACCAAAGCGATGATCGACGAAAGCATCCGCCTGCTCGACAGCCGCACGATCACCACCAACCAGGTG GAGTGCCACGTCTATATGCAGAACCGGCCGATCATCGATTATTGCCAAAACCTCGGTATCGCGGTCACGGCCTATTCGCC GCTCGCGCGCGGCGCCGTCGTCGGCGACCCGGTTCTTGAAGAGATCGGCAAGGCCCGCGGCGCCACCAGCGACCAGATCG CGCTCGCCTTCCTGATCGCCAAGGGGCATGTCGTCATCCCGTCTTCCAGCAAGAGGGGCCGCATCATGCAGAATTTCGAA GCCCGCAAGATTACGCTGACGCCGGCCGAGATCAAACGGATCGAGGGCCTCGAGAAGGGCATGCGACTGGTAAACGGCGA TTGGTGCCCCGTTTGGGACGTGTGA
Upstream 100 bases:
>100_bases GCCCCCTACAAATCGCTGCACGTCGCCGGTTTCTGACGATAGCTCCGCGCGGGTTTTCAGACGCACAGTAACGTTTTGGA TTGCGGCATAGGAAGAGATC
Downstream 100 bases:
>100_bases AGGCAGTTCGCTGCTCGTTCTTTCACCGCCGTCGACATCGAAATGACAGCCGCCGCTGATGCCGCAGTTGTGTTCAAAGA GGAGATGAATATGGATTATC
Product: putative aldo-keto reductase/oxidase
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 274; Mature: 273
Protein sequence:
>274_residues MTKEAMVAGIPQMGYGTWNRSADEAHQGVIWALEAGCRHIDTAQGYGNEQDVARGIKDAGVARNEIFITTKVKPDNYGPG AVMPSVRESLEKLRVDQVDLLLLHWPSPHNKYPLADYLGQFAEVFDSGLARQIGVSNFTKAMIDESIRLLDSRTITTNQV ECHVYMQNRPIIDYCQNLGIAVTAYSPLARGAVVGDPVLEEIGKARGATSDQIALAFLIAKGHVVIPSSSKRGRIMQNFE ARKITLTPAEIKRIEGLEKGMRLVNGDWCPVWDV
Sequences:
>Translated_274_residues MTKEAMVAGIPQMGYGTWNRSADEAHQGVIWALEAGCRHIDTAQGYGNEQDVARGIKDAGVARNEIFITTKVKPDNYGPG AVMPSVRESLEKLRVDQVDLLLLHWPSPHNKYPLADYLGQFAEVFDSGLARQIGVSNFTKAMIDESIRLLDSRTITTNQV ECHVYMQNRPIIDYCQNLGIAVTAYSPLARGAVVGDPVLEEIGKARGATSDQIALAFLIAKGHVVIPSSSKRGRIMQNFE ARKITLTPAEIKRIEGLEKGMRLVNGDWCPVWDV >Mature_273_residues TKEAMVAGIPQMGYGTWNRSADEAHQGVIWALEAGCRHIDTAQGYGNEQDVARGIKDAGVARNEIFITTKVKPDNYGPGA VMPSVRESLEKLRVDQVDLLLLHWPSPHNKYPLADYLGQFAEVFDSGLARQIGVSNFTKAMIDESIRLLDSRTITTNQVE CHVYMQNRPIIDYCQNLGIAVTAYSPLARGAVVGDPVLEEIGKARGATSDQIALAFLIAKGHVVIPSSSKRGRIMQNFEA RKITLTPAEIKRIEGLEKGMRLVNGDWCPVWDV
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI310109922, Length=278, Percent_Identity=32.7338129496403, Blast_Score=147, Evalue=1e-35, Organism=Homo sapiens, GI24497577, Length=287, Percent_Identity=29.616724738676, Blast_Score=145, Evalue=5e-35, Organism=Homo sapiens, GI5174391, Length=287, Percent_Identity=29.616724738676, Blast_Score=145, Evalue=5e-35, Organism=Homo sapiens, GI223468663, Length=293, Percent_Identity=30.3754266211604, Blast_Score=142, Evalue=3e-34, Organism=Homo sapiens, GI310109920, Length=295, Percent_Identity=31.864406779661, Blast_Score=142, Evalue=4e-34, Organism=Homo sapiens, GI45446745, Length=295, Percent_Identity=31.864406779661, Blast_Score=141, Evalue=5e-34, Organism=Homo sapiens, GI4503285, Length=295, Percent_Identity=31.864406779661, Blast_Score=141, Evalue=5e-34, Organism=Homo sapiens, GI5453543, Length=295, Percent_Identity=32.2033898305085, Blast_Score=141, Evalue=6e-34, Organism=Homo sapiens, GI93277124, Length=305, Percent_Identity=30.1639344262295, Blast_Score=140, Evalue=2e-33, Organism=Homo sapiens, GI24497583, Length=295, Percent_Identity=31.1864406779661, Blast_Score=135, Evalue=5e-32, Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=31.986531986532, Blast_Score=132, Evalue=5e-31, Organism=Homo sapiens, GI5174695, Length=294, Percent_Identity=30.952380952381, Blast_Score=129, Evalue=2e-30, Organism=Homo sapiens, GI291291012, Length=271, Percent_Identity=29.8892988929889, Blast_Score=122, Evalue=3e-28, Organism=Homo sapiens, GI4502049, Length=291, Percent_Identity=29.2096219931272, Blast_Score=118, Evalue=5e-27, Organism=Homo sapiens, GI300116271, Length=277, Percent_Identity=30.6859205776173, Blast_Score=114, Evalue=8e-26, Organism=Homo sapiens, GI300116273, Length=270, Percent_Identity=30, Blast_Score=112, Evalue=2e-25, Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=33.5483870967742, Blast_Score=87, Evalue=1e-17, Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=37.5, Blast_Score=75, Evalue=7e-14, Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=37.5, Blast_Score=74, Evalue=1e-13, Organism=Escherichia coli, GI1786400, Length=266, Percent_Identity=39.4736842105263, Blast_Score=204, Evalue=4e-54, Organism=Escherichia coli, GI87082198, Length=258, Percent_Identity=36.046511627907, Blast_Score=149, Evalue=3e-37, Organism=Escherichia coli, GI1788081, Length=262, Percent_Identity=28.2442748091603, Blast_Score=100, Evalue=2e-22, Organism=Escherichia coli, GI87081735, Length=296, Percent_Identity=25.6756756756757, Blast_Score=82, Evalue=4e-17, Organism=Escherichia coli, GI1789375, Length=307, Percent_Identity=22.1498371335505, Blast_Score=67, Evalue=9e-13, Organism=Escherichia coli, GI1787674, Length=248, Percent_Identity=24.5967741935484, Blast_Score=64, Evalue=1e-11, Organism=Escherichia coli, GI1788070, Length=217, Percent_Identity=26.2672811059908, Blast_Score=62, Evalue=3e-11, Organism=Caenorhabditis elegans, GI17550248, Length=289, Percent_Identity=31.4878892733564, Blast_Score=139, Evalue=1e-33, Organism=Caenorhabditis elegans, GI17552492, Length=272, Percent_Identity=30.8823529411765, Blast_Score=131, Evalue=3e-31, Organism=Caenorhabditis elegans, GI17561298, Length=281, Percent_Identity=29.5373665480427, Blast_Score=127, Evalue=5e-30, Organism=Caenorhabditis elegans, GI17537077, Length=290, Percent_Identity=32.0689655172414, Blast_Score=127, Evalue=7e-30, Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=32.0689655172414, Blast_Score=127, Evalue=8e-30, Organism=Caenorhabditis elegans, GI17566692, Length=284, Percent_Identity=29.9295774647887, Blast_Score=119, Evalue=2e-27, Organism=Caenorhabditis elegans, GI17538386, Length=264, Percent_Identity=29.9242424242424, Blast_Score=117, Evalue=6e-27, Organism=Caenorhabditis elegans, GI17564128, Length=301, Percent_Identity=30.8970099667774, Blast_Score=114, Evalue=6e-26, Organism=Caenorhabditis elegans, GI17537079, Length=295, Percent_Identity=29.1525423728814, Blast_Score=113, Evalue=1e-25, Organism=Caenorhabditis elegans, GI71998625, Length=263, Percent_Identity=28.5171102661597, Blast_Score=108, Evalue=3e-24, Organism=Caenorhabditis elegans, GI17561300, Length=261, Percent_Identity=27.5862068965517, Blast_Score=98, Evalue=4e-21, Organism=Caenorhabditis elegans, GI17562292, Length=247, Percent_Identity=29.9595141700405, Blast_Score=90, Evalue=2e-18, Organism=Saccharomyces cerevisiae, GI6324694, Length=298, Percent_Identity=34.2281879194631, Blast_Score=146, Evalue=3e-36, Organism=Saccharomyces cerevisiae, GI6320576, Length=293, Percent_Identity=32.7645051194539, Blast_Score=144, Evalue=2e-35, Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=28.4313725490196, Blast_Score=115, Evalue=6e-27, Organism=Saccharomyces cerevisiae, GI6322556, Length=254, Percent_Identity=29.1338582677165, Blast_Score=112, Evalue=5e-26, Organism=Saccharomyces cerevisiae, GI6319625, Length=273, Percent_Identity=32.6007326007326, Blast_Score=105, Evalue=6e-24, Organism=Saccharomyces cerevisiae, GI6320079, Length=245, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-17, Organism=Drosophila melanogaster, GI24644950, Length=297, Percent_Identity=36.7003367003367, Blast_Score=167, Evalue=1e-41, Organism=Drosophila melanogaster, GI221378297, Length=317, Percent_Identity=34.0694006309148, Blast_Score=159, Evalue=2e-39, Organism=Drosophila melanogaster, GI20129731, Length=280, Percent_Identity=33.2142857142857, Blast_Score=142, Evalue=3e-34, Organism=Drosophila melanogaster, GI24662785, Length=288, Percent_Identity=35.4166666666667, Blast_Score=134, Evalue=5e-32, Organism=Drosophila melanogaster, GI24662781, Length=288, Percent_Identity=35.0694444444444, Blast_Score=134, Evalue=7e-32, Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=30.5263157894737, Blast_Score=131, Evalue=5e-31, Organism=Drosophila melanogaster, GI24662789, Length=265, Percent_Identity=30.9433962264151, Blast_Score=129, Evalue=2e-30, Organism=Drosophila melanogaster, GI45553081, Length=289, Percent_Identity=31.1418685121107, Blast_Score=124, Evalue=5e-29, Organism=Drosophila melanogaster, GI24657054, Length=290, Percent_Identity=31.0344827586207, Blast_Score=122, Evalue=3e-28, Organism=Drosophila melanogaster, GI21356425, Length=287, Percent_Identity=28.9198606271777, Blast_Score=120, Evalue=9e-28, Organism=Drosophila melanogaster, GI281366140, Length=263, Percent_Identity=30.7984790874525, Blast_Score=107, Evalue=6e-24,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 30191; Mature: 30060
Theoretical pI: Translated: 6.79; Mature: 6.79
Prosite motif: PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 4.4 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 4.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTKEAMVAGIPQMGYGTWNRSADEAHQGVIWALEAGCRHIDTAQGYGNEQDVARGIKDAG CCCCCHHHCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCC VARNEIFITTKVKPDNYGPGAVMPSVRESLEKLRVDQVDLLLLHWPSPHNKYPLADYLGQ CCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHH FAEVFDSGLARQIGVSNFTKAMIDESIRLLDSRTITTNQVECHVYMQNRPIIDYCQNLGI HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEECCEEEEEEEECCCCHHHHHHCCCE AVTAYSPLARGAVVGDPVLEEIGKARGATSDQIALAFLIAKGHVVIPSSSKRGRIMQNFE EEEECCHHHCCCCCCCHHHHHHHHHCCCCCCHHEEEEEEECCCEEECCCCCCCCHHHCCC ARKITLTPAEIKRIEGLEKGMRLVNGDWCPVWDV CCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCC >Mature Secondary Structure TKEAMVAGIPQMGYGTWNRSADEAHQGVIWALEAGCRHIDTAQGYGNEQDVARGIKDAG CCCCHHHCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCC VARNEIFITTKVKPDNYGPGAVMPSVRESLEKLRVDQVDLLLLHWPSPHNKYPLADYLGQ CCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHH FAEVFDSGLARQIGVSNFTKAMIDESIRLLDSRTITTNQVECHVYMQNRPIIDYCQNLGI HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEECCEEEEEEEECCCCHHHHHHCCCE AVTAYSPLARGAVVGDPVLEEIGKARGATSDQIALAFLIAKGHVVIPSSSKRGRIMQNFE EEEECCHHHCCCCCCCHHHHHHHHHCCCCCCHHEEEEEEECCCEEECCCCCCCCHHHCCC ARKITLTPAEIKRIEGLEKGMRLVNGDWCPVWDV CCEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11586360; 12142430 [H]