| Definition | Rhizobium leguminosarum bv. viciae 3841 plasmid pRL12, complete sequence. |
|---|---|
| Accession | NC_008378 |
| Length | 870,021 |
Click here to switch to the map view.
The map label for this gene is braF [H]
Identifier: 116248774
GI number: 116248774
Start: 93863
End: 94630
Strand: Reverse
Name: braF [H]
Synonym: pRL120098
Alternate gene names: 116248774
Gene position: 94630-93863 (Counterclockwise)
Preceding gene: 116248775
Following gene: 116248773
Centisome position: 10.88
GC content: 64.32
Gene sequence:
>768_bases TTGGCGCGGGGCCTGCTAGGGGCCCCGCCTATTCTGGGAGGAATGGGTTTGGCAATTCTCGAACTCGATGGGGTCTCGAA GAAATTCGGCGCATTGACGGTCGCCGAGCAGATTTCCTTTGCCGTCGGCGAAGGAGAGGCGCTGGGCATTATCGGGCCGA ACGGCGCCGGCAAGTCGACGCTCTTCAATCTGATCACCGGTAATATCCCTGCCGATAGCGGCAGCATCCGCTTTCTCGGC AGCGATGTGACCCGCACGCCGCCGATGGCGCGCTGCCTGTCGGGCATGGGCCGCACCTTCCAGATCCCGCAGCCGTTCGA GAAGCTGACGGTCTTCGAAAACCTCCTGGTCGCCGGCGCCTTCGGATCGCGGCGGCGCGAGGCCGAGGTGTCGGACCGCT GCGCCGAAATCCTGGTGGACACTGGGCTGATCGATCAGGCGAATGTGCTGGCCGGAAGCCTGTCGCTGCTGCAGCGCAAG CGGCTGGAACTGGCCCGGGCGCTGGCGACGGAACCGAAGCTGCTGCTGCTCGATGAAATCGCCGGCGGACTGACCGAGGG CGAGTGCCGGTCGCTGGTGGCCACCATCCGCGCCATTCATGCGCGCGGCGTTGCCGTCATCTGGATCGAGCACGTGCTGC ATGCGCTGAATTCCGTCGTCGAGCGGCTGCTGGTGCTGCATTTCGGCAAAGTCATCGGCATCGGCAAGCCGGAGGACATT ATGGCGTCGCGCGATGTGCGTGAAATCTATCTGGGGATCGAGATCTGA
Upstream 100 bases:
>100_bases GCGGTTGAAGGACGGCGGCGGCTACGATCTCGTCATCACCGACAACAAGACGGCGCCGAACATTCCGGTCGGCGGCAAAA TGGAAGCGATCGCCTGATTT
Downstream 100 bases:
>100_bases TGGCGCTGCTCGAAACCAGAGGCTTGACCGCTTCCTACGGCGACTTCCAGGCGCTATTCGGCGTCGATATCATGGTCGGC TCCGGCGAGACCATCGCCAT
Product: putative ATP-binding ABC transporter component
Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]
Alternate protein names: NA
Number of amino acids: Translated: 255; Mature: 254
Protein sequence:
>255_residues MARGLLGAPPILGGMGLAILELDGVSKKFGALTVAEQISFAVGEGEALGIIGPNGAGKSTLFNLITGNIPADSGSIRFLG SDVTRTPPMARCLSGMGRTFQIPQPFEKLTVFENLLVAGAFGSRRREAEVSDRCAEILVDTGLIDQANVLAGSLSLLQRK RLELARALATEPKLLLLDEIAGGLTEGECRSLVATIRAIHARGVAVIWIEHVLHALNSVVERLLVLHFGKVIGIGKPEDI MASRDVREIYLGIEI
Sequences:
>Translated_255_residues MARGLLGAPPILGGMGLAILELDGVSKKFGALTVAEQISFAVGEGEALGIIGPNGAGKSTLFNLITGNIPADSGSIRFLG SDVTRTPPMARCLSGMGRTFQIPQPFEKLTVFENLLVAGAFGSRRREAEVSDRCAEILVDTGLIDQANVLAGSLSLLQRK RLELARALATEPKLLLLDEIAGGLTEGECRSLVATIRAIHARGVAVIWIEHVLHALNSVVERLLVLHFGKVIGIGKPEDI MASRDVREIYLGIEI >Mature_254_residues ARGLLGAPPILGGMGLAILELDGVSKKFGALTVAEQISFAVGEGEALGIIGPNGAGKSTLFNLITGNIPADSGSIRFLGS DVTRTPPMARCLSGMGRTFQIPQPFEKLTVFENLLVAGAFGSRRREAEVSDRCAEILVDTGLIDQANVLAGSLSLLQRKR LELARALATEPKLLLLDEIAGGLTEGECRSLVATIRAIHARGVAVIWIEHVLHALNSVVERLLVLHFGKVIGIGKPEDIM ASRDVREIYLGIEI
Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]
COG id: COG0411
COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component
Gene ontology:
Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ABC transporter domain [H]
Homologues:
Organism=Homo sapiens, GI46592956, Length=213, Percent_Identity=30.9859154929577, Blast_Score=80, Evalue=2e-15, Organism=Homo sapiens, GI46592978, Length=213, Percent_Identity=30.9859154929577, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI46592964, Length=213, Percent_Identity=30.9859154929577, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI46592971, Length=213, Percent_Identity=30.9859154929577, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI46592915, Length=213, Percent_Identity=30.9859154929577, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI46592898, Length=213, Percent_Identity=30.9859154929577, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI150417984, Length=210, Percent_Identity=29.0476190476191, Blast_Score=77, Evalue=2e-14, Organism=Homo sapiens, GI27262624, Length=202, Percent_Identity=30.6930693069307, Blast_Score=77, Evalue=2e-14, Organism=Homo sapiens, GI27262626, Length=202, Percent_Identity=30.6930693069307, Blast_Score=77, Evalue=2e-14, Organism=Homo sapiens, GI27881501, Length=217, Percent_Identity=27.1889400921659, Blast_Score=76, Evalue=3e-14, Organism=Homo sapiens, GI30795238, Length=217, Percent_Identity=27.1889400921659, Blast_Score=76, Evalue=3e-14, Organism=Homo sapiens, GI153792144, Length=166, Percent_Identity=31.9277108433735, Blast_Score=73, Evalue=2e-13, Organism=Homo sapiens, GI105990541, Length=219, Percent_Identity=29.2237442922374, Blast_Score=73, Evalue=2e-13, Organism=Homo sapiens, GI215820660, Length=212, Percent_Identity=28.3018867924528, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI20143975, Length=212, Percent_Identity=28.3018867924528, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI31657092, Length=228, Percent_Identity=26.7543859649123, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI171184400, Length=235, Percent_Identity=29.3617021276596, Blast_Score=65, Evalue=6e-11, Organism=Escherichia coli, GI1789864, Length=255, Percent_Identity=33.7254901960784, Blast_Score=132, Evalue=2e-32, Organism=Escherichia coli, GI1789593, Length=236, Percent_Identity=30.9322033898305, Blast_Score=120, Evalue=8e-29, Organism=Escherichia coli, GI1787792, Length=225, Percent_Identity=30.2222222222222, Blast_Score=105, Evalue=3e-24, Organism=Escherichia coli, GI87081782, Length=234, Percent_Identity=32.9059829059829, Blast_Score=104, Evalue=6e-24, Organism=Escherichia coli, GI1790190, Length=218, Percent_Identity=28.4403669724771, Blast_Score=103, Evalue=1e-23, Organism=Escherichia coli, GI87081791, Length=233, Percent_Identity=29.1845493562232, Blast_Score=102, Evalue=3e-23, Organism=Escherichia coli, GI1788761, Length=230, Percent_Identity=30.8695652173913, Blast_Score=100, Evalue=7e-23, Organism=Escherichia coli, GI1787370, Length=226, Percent_Identity=29.646017699115, Blast_Score=99, Evalue=3e-22, Organism=Escherichia coli, GI1789991, Length=248, Percent_Identity=28.6290322580645, Blast_Score=99, Evalue=3e-22, Organism=Escherichia coli, GI48995001, Length=218, Percent_Identity=28.8990825688073, Blast_Score=95, Evalue=6e-21, Organism=Escherichia coli, GI87082268, Length=238, Percent_Identity=28.9915966386555, Blast_Score=94, Evalue=6e-21, Organism=Escherichia coli, GI1790467, Length=225, Percent_Identity=30.6666666666667, Blast_Score=94, Evalue=1e-20, Organism=Escherichia coli, GI1788897, Length=219, Percent_Identity=29.6803652968037, Blast_Score=92, Evalue=3e-20, Organism=Escherichia coli, GI1787029, Length=223, Percent_Identity=30.0448430493274, Blast_Score=91, Evalue=7e-20, Organism=Escherichia coli, GI87081709, Length=226, Percent_Identity=27.8761061946903, Blast_Score=90, Evalue=1e-19, Organism=Escherichia coli, GI48994943, Length=241, Percent_Identity=30.2904564315353, Blast_Score=90, Evalue=1e-19, Organism=Escherichia coli, GI87081834, Length=208, Percent_Identity=29.8076923076923, Blast_Score=89, Evalue=3e-19, Organism=Escherichia coli, GI1787712, Length=225, Percent_Identity=26.6666666666667, Blast_Score=89, Evalue=3e-19, Organism=Escherichia coli, GI1790525, Length=249, Percent_Identity=25.3012048192771, Blast_Score=87, Evalue=1e-18, Organism=Escherichia coli, GI1789672, Length=227, Percent_Identity=24.6696035242291, Blast_Score=86, Evalue=2e-18, Organism=Escherichia coli, GI1788450, Length=223, Percent_Identity=30.0448430493274, Blast_Score=86, Evalue=3e-18, Organism=Escherichia coli, GI1788472, Length=220, Percent_Identity=25.9090909090909, Blast_Score=84, Evalue=1e-17, Organism=Escherichia coli, GI1786319, Length=219, Percent_Identity=29.2237442922374, Blast_Score=83, Evalue=1e-17, Organism=Escherichia coli, GI1789873, Length=224, Percent_Identity=29.9107142857143, Blast_Score=82, Evalue=3e-17, Organism=Escherichia coli, GI1786253, Length=238, Percent_Identity=30.2521008403361, Blast_Score=81, Evalue=6e-17, Organism=Escherichia coli, GI1786872, Length=222, Percent_Identity=28.3783783783784, Blast_Score=80, Evalue=9e-17, Organism=Escherichia coli, GI1790544, Length=243, Percent_Identity=27.5720164609054, Blast_Score=80, Evalue=1e-16, Organism=Escherichia coli, GI1786398, Length=221, Percent_Identity=28.5067873303167, Blast_Score=79, Evalue=2e-16, Organism=Escherichia coli, GI1788225, Length=234, Percent_Identity=26.4957264957265, Blast_Score=79, Evalue=2e-16, Organism=Escherichia coli, GI1786698, Length=205, Percent_Identity=30.7317073170732, Blast_Score=78, Evalue=7e-16, Organism=Escherichia coli, GI1789032, Length=230, Percent_Identity=28.2608695652174, Blast_Score=77, Evalue=9e-16, Organism=Escherichia coli, GI1788210, Length=221, Percent_Identity=25.3393665158371, Blast_Score=73, Evalue=2e-14, Organism=Escherichia coli, GI1787105, Length=200, Percent_Identity=29.5, Blast_Score=72, Evalue=3e-14, Organism=Escherichia coli, GI1786563, Length=199, Percent_Identity=32.1608040201005, Blast_Score=71, Evalue=7e-14, Organism=Escherichia coli, GI1787089, Length=221, Percent_Identity=25.7918552036199, Blast_Score=71, Evalue=9e-14, Organism=Escherichia coli, GI1787500, Length=222, Percent_Identity=26.1261261261261, Blast_Score=70, Evalue=1e-13, Organism=Escherichia coli, GI1787547, Length=243, Percent_Identity=25.5144032921811, Blast_Score=70, Evalue=2e-13, Organism=Escherichia coli, GI1787758, Length=209, Percent_Identity=24.4019138755981, Blast_Score=69, Evalue=4e-13, Organism=Escherichia coli, GI1790162, Length=232, Percent_Identity=25.4310344827586, Blast_Score=68, Evalue=7e-13, Organism=Escherichia coli, GI1786803, Length=229, Percent_Identity=25.764192139738, Blast_Score=67, Evalue=9e-13, Organism=Escherichia coli, GI87082267, Length=232, Percent_Identity=26.2931034482759, Blast_Score=67, Evalue=2e-12, Organism=Escherichia coli, GI1790739, Length=241, Percent_Identity=27.8008298755187, Blast_Score=66, Evalue=2e-12, Organism=Escherichia coli, GI1786345, Length=240, Percent_Identity=27.5, Blast_Score=65, Evalue=3e-12, Organism=Escherichia coli, GI48994997, Length=241, Percent_Identity=25.7261410788382, Blast_Score=64, Evalue=9e-12, Organism=Escherichia coli, GI1786653, Length=226, Percent_Identity=27.8761061946903, Blast_Score=64, Evalue=1e-11, Organism=Escherichia coli, GI2367384, Length=185, Percent_Identity=30.2702702702703, Blast_Score=64, Evalue=1e-11, Organism=Escherichia coli, GI145693107, Length=247, Percent_Identity=25.9109311740891, Blast_Score=63, Evalue=2e-11, Organism=Escherichia coli, GI1787164, Length=233, Percent_Identity=27.8969957081545, Blast_Score=62, Evalue=3e-11, Organism=Escherichia coli, GI1789962, Length=218, Percent_Identity=23.394495412844, Blast_Score=61, Evalue=6e-11, Organism=Escherichia coli, GI1789586, Length=245, Percent_Identity=28.1632653061224, Blast_Score=61, Evalue=6e-11, Organism=Caenorhabditis elegans, GI115533608, Length=208, Percent_Identity=29.8076923076923, Blast_Score=83, Evalue=1e-16, Organism=Caenorhabditis elegans, GI17510237, Length=224, Percent_Identity=24.1071428571429, Blast_Score=76, Evalue=1e-14, Organism=Caenorhabditis elegans, GI71983516, Length=231, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=2e-14, Organism=Caenorhabditis elegans, GI71983510, Length=231, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=2e-14, Organism=Caenorhabditis elegans, GI71983523, Length=231, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=2e-14, Organism=Caenorhabditis elegans, GI193211015, Length=220, Percent_Identity=26.8181818181818, Blast_Score=69, Evalue=2e-12, Organism=Caenorhabditis elegans, GI71996809, Length=220, Percent_Identity=26.8181818181818, Blast_Score=69, Evalue=2e-12, Organism=Caenorhabditis elegans, GI193211017, Length=220, Percent_Identity=26.8181818181818, Blast_Score=69, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17551700, Length=211, Percent_Identity=29.8578199052133, Blast_Score=67, Evalue=8e-12, Organism=Caenorhabditis elegans, GI17551702, Length=211, Percent_Identity=29.8578199052133, Blast_Score=67, Evalue=8e-12, Organism=Caenorhabditis elegans, GI17565938, Length=245, Percent_Identity=26.9387755102041, Blast_Score=66, Evalue=1e-11, Organism=Caenorhabditis elegans, GI212646699, Length=217, Percent_Identity=25.3456221198157, Blast_Score=66, Evalue=1e-11, Organism=Caenorhabditis elegans, GI25146777, Length=221, Percent_Identity=26.6968325791855, Blast_Score=66, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17565586, Length=210, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=3e-11, Organism=Saccharomyces cerevisiae, GI6323217, Length=216, Percent_Identity=25.9259259259259, Blast_Score=63, Evalue=4e-11, Organism=Drosophila melanogaster, GI28574150, Length=214, Percent_Identity=28.0373831775701, Blast_Score=76, Evalue=2e-14, Organism=Drosophila melanogaster, GI116007328, Length=214, Percent_Identity=28.0373831775701, Blast_Score=76, Evalue=2e-14, Organism=Drosophila melanogaster, GI24666092, Length=230, Percent_Identity=25.6521739130435, Blast_Score=73, Evalue=2e-13, Organism=Drosophila melanogaster, GI19920532, Length=196, Percent_Identity=32.1428571428571, Blast_Score=72, Evalue=4e-13, Organism=Drosophila melanogaster, GI24580930, Length=196, Percent_Identity=32.1428571428571, Blast_Score=72, Evalue=4e-13, Organism=Drosophila melanogaster, GI161076684, Length=189, Percent_Identity=31.7460317460317, Blast_Score=71, Evalue=6e-13, Organism=Drosophila melanogaster, GI24581617, Length=189, Percent_Identity=31.7460317460317, Blast_Score=71, Evalue=6e-13, Organism=Drosophila melanogaster, GI24581615, Length=189, Percent_Identity=31.7460317460317, Blast_Score=71, Evalue=6e-13, Organism=Drosophila melanogaster, GI161076682, Length=189, Percent_Identity=31.7460317460317, Blast_Score=71, Evalue=6e-13, Organism=Drosophila melanogaster, GI221512771, Length=212, Percent_Identity=25.9433962264151, Blast_Score=70, Evalue=2e-12, Organism=Drosophila melanogaster, GI28574533, Length=234, Percent_Identity=28.2051282051282, Blast_Score=67, Evalue=8e-12, Organism=Drosophila melanogaster, GI45552805, Length=179, Percent_Identity=31.2849162011173, Blast_Score=67, Evalue=2e-11, Organism=Drosophila melanogaster, GI17647219, Length=179, Percent_Identity=31.2849162011173, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI62484234, Length=213, Percent_Identity=30.5164319248826, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI24643674, Length=226, Percent_Identity=26.5486725663717, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI85724864, Length=223, Percent_Identity=27.3542600896861, Blast_Score=65, Evalue=4e-11, Organism=Drosophila melanogaster, GI24580555, Length=223, Percent_Identity=27.3542600896861, Blast_Score=65, Evalue=4e-11, Organism=Drosophila melanogaster, GI85816269, Length=223, Percent_Identity=27.3542600896861, Blast_Score=65, Evalue=4e-11, Organism=Drosophila melanogaster, GI24580553, Length=223, Percent_Identity=27.3542600896861, Blast_Score=65, Evalue=4e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003439 - InterPro: IPR017871 - InterPro: IPR003593 [H]
Pfam domain/function: PF00005 ABC_tran [H]
EC number: NA
Molecular weight: Translated: 27067; Mature: 26936
Theoretical pI: Translated: 6.52; Mature: 6.52
Prosite motif: PS50893 ABC_TRANSPORTER_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 1.6 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MARGLLGAPPILGGMGLAILELDGVSKKFGALTVAEQISFAVGEGEALGIIGPNGAGKST CCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHEECCCCEEEEECCCCCCHHH LFNLITGNIPADSGSIRFLGSDVTRTPPMARCLSGMGRTFQIPQPFEKLTVFENLLVAGA HHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCC FGSRRREAEVSDRCAEILVDTGLIDQANVLAGSLSLLQRKRLELARALATEPKLLLLDEI CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHH AGGLTEGECRSLVATIRAIHARGVAVIWIEHVLHALNSVVERLLVLHFGKVIGIGKPEDI HCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH MASRDVREIYLGIEI HHHCCHHHEEEEECC >Mature Secondary Structure ARGLLGAPPILGGMGLAILELDGVSKKFGALTVAEQISFAVGEGEALGIIGPNGAGKST CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHEECCCCEEEEECCCCCCHHH LFNLITGNIPADSGSIRFLGSDVTRTPPMARCLSGMGRTFQIPQPFEKLTVFENLLVAGA HHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCC FGSRRREAEVSDRCAEILVDTGLIDQANVLAGSLSLLQRKRLELARALATEPKLLLLDEI CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHH AGGLTEGECRSLVATIRAIHARGVAVIWIEHVLHALNSVVERLLVLHFGKVIGIGKPEDI HCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH MASRDVREIYLGIEI HHHCCHHHEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]
Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 7.0
TargetDB status: NA
Availability: NA
References: 2120183; 10984043 [H]