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The map label for this gene is yckA [H]

Identifier: 116248749

GI number: 116248749

Start: 74826

End: 75485

Strand: Direct

Name: yckA [H]

Synonym: pRL120073

Alternate gene names: 116248749

Gene position: 74826-75485 (Clockwise)

Preceding gene: 116248748

Following gene: 116248750

Centisome position: 8.6

GC content: 61.82

Gene sequence:

>660_bases
ATGTCCCACACCTTTCTCGAACAACTCTGGATCGCCCGCTACGTCATCATGAACGGCATCGGCGTGACCGTGTCGATCTC
CCTGCTGGCCATCCTCGCGGGTTCCATACTCGGCGTTTTCGTCGGTTTGGCGCTCGTCTATGGCGGCGTTGTTTTGCGTC
TGGCCGTGCGCGCCTATACGGATATCATCCGTGGCACGCCGGTGCTCGTGCTGGTTCTGGCGAGCTACTATGTCTCCGCC
GCCGTCGGCCTCGATCTCGGGCCGTTCTCGGCCGGCGTGCTCGCCCTTGCCATCTTCTGCTCCTCCCATGTCGGCGAAAT
CGTGCGCGGGGCGCTTCAGGCAATCCCGAAAGGGCAGACCGAAGCTGCCAAGGCGATCGGCCTGACCTTTACCCAGACCT
TCACCTCGGTGCTGTGGCCGCAGGCCATGCGCCAGTGCCTGCCGGCCTGGGTGAATACGGCGGCCGAGATGGTGAAGGCC
TCGACGCTGCTCTCCGTCATCGGCGTCGCAGAACTTCTCCTGCGCACGCAGGAGATCATCTCCCGCAATTTCATGAGCCT
CCAGTTCTATTTCCTGGCTGGCGGTCTCTATTTCATCATCAACTACGGCATCGAGCACTTCGGCAAATATGTCGAGCGCA
AGACCGCCCTGCCGTCCTGA

Upstream 100 bases:

>100_bases
CCTGGATGGTCACATCAGCGCTCTATGTCGCGACCTGTTTCCTCATCGCCGCCGTGATGCGCTTCGTCGAGCGCCGCCTG
GCCCTGCCGAGATAGACCCG

Downstream 100 bases:

>100_bases
GGAGTTCCCCCGATGGCCAAGACCATTCTCGACATCCAGGGTCTGCGCAAGACTTACGGCATCCACGAAGTCCTCAAGGG
CGTCGATTGCGCCGTTGAGG

Product: putative permease component of ABC transporter

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSHTFLEQLWIARYVIMNGIGVTVSISLLAILAGSILGVFVGLALVYGGVVLRLAVRAYTDIIRGTPVLVLVLASYYVSA
AVGLDLGPFSAGVLALAIFCSSHVGEIVRGALQAIPKGQTEAAKAIGLTFTQTFTSVLWPQAMRQCLPAWVNTAAEMVKA
STLLSVIGVAELLLRTQEIISRNFMSLQFYFLAGGLYFIINYGIEHFGKYVERKTALPS

Sequences:

>Translated_219_residues
MSHTFLEQLWIARYVIMNGIGVTVSISLLAILAGSILGVFVGLALVYGGVVLRLAVRAYTDIIRGTPVLVLVLASYYVSA
AVGLDLGPFSAGVLALAIFCSSHVGEIVRGALQAIPKGQTEAAKAIGLTFTQTFTSVLWPQAMRQCLPAWVNTAAEMVKA
STLLSVIGVAELLLRTQEIISRNFMSLQFYFLAGGLYFIINYGIEHFGKYVERKTALPS
>Mature_218_residues
SHTFLEQLWIARYVIMNGIGVTVSISLLAILAGSILGVFVGLALVYGGVVLRLAVRAYTDIIRGTPVLVLVLASYYVSAA
VGLDLGPFSAGVLALAIFCSSHVGEIVRGALQAIPKGQTEAAKAIGLTFTQTFTSVLWPQAMRQCLPAWVNTAAEMVKAS
TLLSVIGVAELLLRTQEIISRNFMSLQFYFLAGGLYFIINYGIEHFGKYVERKTALPS

Specific function: Part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787030, Length=211, Percent_Identity=33.175355450237, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788226, Length=208, Percent_Identity=30.2884615384615, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1787087, Length=225, Percent_Identity=25.7777777777778, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1788646, Length=196, Percent_Identity=29.5918367346939, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082239, Length=202, Percent_Identity=27.7227722772277, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786873, Length=210, Percent_Identity=24.7619047619048, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786874, Length=216, Percent_Identity=28.2407407407407, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788645, Length=218, Percent_Identity=26.605504587156, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787086, Length=209, Percent_Identity=23.9234449760766, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI48994930, Length=129, Percent_Identity=29.4573643410853, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 23562; Mature: 23431

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTFLEQLWIARYVIMNGIGVTVSISLLAILAGSILGVFVGLALVYGGVVLRLAVRAYT
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIIRGTPVLVLVLASYYVSAAVGLDLGPFSAGVLALAIFCSSHVGEIVRGALQAIPKGQT
HHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EAAKAIGLTFTQTFTSVLWPQAMRQCLPAWVNTAAEMVKASTLLSVIGVAELLLRTQEII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRNFMSLQFYFLAGGLYFIINYGIEHFGKYVERKTALPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SHTFLEQLWIARYVIMNGIGVTVSISLLAILAGSILGVFVGLALVYGGVVLRLAVRAYT
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIIRGTPVLVLVLASYYVSAAVGLDLGPFSAGVLALAIFCSSHVGEIVRGALQAIPKGQT
HHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EAAKAIGLTFTQTFTSVLWPQAMRQCLPAWVNTAAEMVKASTLLSVIGVAELLLRTQEII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRNFMSLQFYFLAGGLYFIINYGIEHFGKYVERKTALPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7704255; 8969502; 9384377 [H]