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The map label for this gene is peb1A [H]

Identifier: 116248747

GI number: 116248747

Start: 73201

End: 74040

Strand: Direct

Name: peb1A [H]

Synonym: pRL120071

Alternate gene names: 116248747

Gene position: 73201-74040 (Clockwise)

Preceding gene: 116248746

Following gene: 116248748

Centisome position: 8.41

GC content: 60.6

Gene sequence:

>840_bases
ATGACCATTTCCTTTCGCACCGGCGTGATGTCGCTGGCCGTCGCCGCTCTGTTGTCCACGCCTGCGCTGGCTGAAGGCAG
CAAGCTCGATGAAGTGCTGGCCCGCGGCCATCTCGTCCTCGGCACGGGCAGCACCAATGCGCCTTGGCACTTCAAGAGCG
CCGACGACAAGCTTCAGGGATTCGACGTCGACATGGGCCACATCATCGCCAAGGCGCTCTTCGGCGATCCTGAAAAGATC
GAGTATGTGAACCAGTCCTCCGACGCGCGCATCCCGAACATTACCACCGACAAAGTCGACATCACCTGCCAGTTCATGAC
CGTTACCGGCGAGCGTGCCCAGCAGGTTGCCTTCACCATTCCTTATTATCGCGAGGGTGTTGGCCTGATGCTCAAGGCGG
ATGGCAAATATGCCGACTACGCCGCCCTCAAGGCGGCAGGCTCCTCCGTTACCATCTCGGTTCTCCAGAACGTCTATGCC
GAGGCGATGGTGCATGCGGCATTGCCTGAGGCGACCGTCGACCAATACGATTCCGTCGACCTGATCTATCAGGCGCTGGA
ATCGGGACGCGCCGATGCTGTCGCCACGGACCAGTCGTCGCTTGCCTGGTACATGACGCAGAATCCGGGCCGTTACAAAG
ATGCCGGCTACGGCTGGAACCCGCAGACCTACGCCTGCGCCGTCAAGCGCGGCGATCAGGATTGGCTGAACTTCGTCAAC
ACCGCCCTGCACGAGGCCATGACCGGCGTCGAATTCGACTTTTACGCCAAGTCCTTCAAGACCTGGTTCGGCAAGGATCT
GGCGCCGCCGCAGATCGGCTTCCCGGTCGAGTTCAAGTAA

Upstream 100 bases:

>100_bases
TGACAATTCATCCTTGATGGCACTTCATAACATTGTTCCAGTAAAACAGACATGCGTCCATTATACTGGAACAAACGGAA
TCATCCATAAGGGGAACGCC

Downstream 100 bases:

>100_bases
CAGCCATGCGGAGCGGGACATGTTTCCCGCTCCGCATTTAAAGGGTGAGAGCATGATGCCGTCCGAAAACCGTTCATATT
TTTCGGCCTCATGCTCTGGT

Product: putative substrate-binding component of ABC transporter

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: CBF1; PEB1 [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTISFRTGVMSLAVAALLSTPALAEGSKLDEVLARGHLVLGTGSTNAPWHFKSADDKLQGFDVDMGHIIAKALFGDPEKI
EYVNQSSDARIPNITTDKVDITCQFMTVTGERAQQVAFTIPYYREGVGLMLKADGKYADYAALKAAGSSVTISVLQNVYA
EAMVHAALPEATVDQYDSVDLIYQALESGRADAVATDQSSLAWYMTQNPGRYKDAGYGWNPQTYACAVKRGDQDWLNFVN
TALHEAMTGVEFDFYAKSFKTWFGKDLAPPQIGFPVEFK

Sequences:

>Translated_279_residues
MTISFRTGVMSLAVAALLSTPALAEGSKLDEVLARGHLVLGTGSTNAPWHFKSADDKLQGFDVDMGHIIAKALFGDPEKI
EYVNQSSDARIPNITTDKVDITCQFMTVTGERAQQVAFTIPYYREGVGLMLKADGKYADYAALKAAGSSVTISVLQNVYA
EAMVHAALPEATVDQYDSVDLIYQALESGRADAVATDQSSLAWYMTQNPGRYKDAGYGWNPQTYACAVKRGDQDWLNFVN
TALHEAMTGVEFDFYAKSFKTWFGKDLAPPQIGFPVEFK
>Mature_278_residues
TISFRTGVMSLAVAALLSTPALAEGSKLDEVLARGHLVLGTGSTNAPWHFKSADDKLQGFDVDMGHIIAKALFGDPEKIE
YVNQSSDARIPNITTDKVDITCQFMTVTGERAQQVAFTIPYYREGVGLMLKADGKYADYAALKAAGSSVTISVLQNVYAE
AMVHAALPEATVDQYDSVDLIYQALESGRADAVATDQSSLAWYMTQNPGRYKDAGYGWNPQTYACAVKRGDQDWLNFVNT
ALHEAMTGVEFDFYAKSFKTWFGKDLAPPQIGFPVEFK

Specific function: Common antigen and a major cell adherence molecule. Most probably involved, with PEB1C, in a binding-protein-dependent transport system for an amino acid. May be involved in binding to intestinal cells [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell surface [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=259, Percent_Identity=30.5019305019305, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788228, Length=262, Percent_Identity=26.3358778625954, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786876, Length=263, Percent_Identity=25.0950570342205, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787088, Length=232, Percent_Identity=26.7241379310345, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787085, Length=236, Percent_Identity=25.4237288135593, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788649, Length=239, Percent_Identity=25.9414225941423, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788648, Length=238, Percent_Identity=25.2100840336134, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30485; Mature: 30353

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISFRTGVMSLAVAALLSTPALAEGSKLDEVLARGHLVLGTGSTNAPWHFKSADDKLQG
CEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCC
FDVDMGHIIAKALFGDPEKIEYVNQSSDARIPNITTDKVDITCQFMTVTGERAQQVAFTI
CCCCHHHHHHHHHCCCHHHHHEECCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEC
PYYREGVGLMLKADGKYADYAALKAAGSSVTISVLQNVYAEAMVHAALPEATVDQYDSVD
CHHHCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCHH
LIYQALESGRADAVATDQSSLAWYMTQNPGRYKDAGYGWNPQTYACAVKRGDQDWLNFVN
HHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHH
TALHEAMTGVEFDFYAKSFKTWFGKDLAPPQIGFPVEFK
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TISFRTGVMSLAVAALLSTPALAEGSKLDEVLARGHLVLGTGSTNAPWHFKSADDKLQG
EEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCC
FDVDMGHIIAKALFGDPEKIEYVNQSSDARIPNITTDKVDITCQFMTVTGERAQQVAFTI
CCCCHHHHHHHHHCCCHHHHHEECCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEC
PYYREGVGLMLKADGKYADYAALKAAGSSVTISVLQNVYAEAMVHAALPEATVDQYDSVD
CHHHCCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCHH
LIYQALESGRADAVATDQSSLAWYMTQNPGRYKDAGYGWNPQTYACAVKRGDQDWLNFVN
HHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHH
TALHEAMTGVEFDFYAKSFKTWFGKDLAPPQIGFPVEFK
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204; 1885571 [H]