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The map label for this gene is eutB [C]

Identifier: 116248728

GI number: 116248728

Start: 51198

End: 52199

Strand: Direct

Name: eutB [C]

Synonym: pRL120052

Alternate gene names: 116248728

Gene position: 51198-52199 (Clockwise)

Preceding gene: 116248727

Following gene: 116248729

Centisome position: 5.88

GC content: 66.37

Gene sequence:

>1002_bases
ATGGTGAGCACCTTGCCCGTTTCGCTGGAGGATATCCGCGCTGCGGCGCGCCGGATTGCCGGCCGCGTGATCGGCACACC
GATGGTGCAGTCCTTTTCCCTTGGCGAATTGGCCGGCGTACCTGTCCATCTCAAGCTCGAACATCATCAGACGACCGGCA
GCTTCAAGCTGCGCGGCGCGACCAATGCGGTGCTCTCCTTGTCGCCGGCCGAGCGCTCGCGCGGCGTCGTCGCGGCCTCG
ACCGGAAATCACGGCCGTGCGCTTGCCTATGCGGCAAAGGCGGAAGGGGCGGTTGCGACCATCTGCATGTCGCGGCTGGT
GCCGGACAACAAGGTTTCGGAAAGCCGCCGCCTCGGTGCCGATGTCCGCATCGTCGGCGGATCGCAGGACGAGGCGCAGC
AGGAAGTCGAGCGGCTGGTTCGCGAGGAGGGGCTGGTGATGGTCCCGCCCTTCGACCATCCTGATGTCGTGGCCGGGCAG
GGGACGCTGGGGCTCGAAATCATCGACATCTTGCCGGAGGCGGCAATCGTGCTGGTGCCGCTCTCGGGCGGCGGCCTTGC
GGCCGGCGTTGCCGCTGCGGTCAAAGGCATCAGTCCGACGACCAAAGTGATCGGCCTGACGATGGAGAAGGGCGCCGCGA
TGAAGGCGAGCCTCGATGCCGGACGGCCGGTGCAGGTCGAGGAGGTATCGAGCCTTGCCGACTCGCTCGGCGGCGGCATC
GGCCTCGACAATCGCGTGACCTTTGCCATGTGCCGCGAACTTCTCGACGACGTCATCCTGCTGACGGAGGTGGAAATCGC
CGTGGGCATGCGCCATGCCTATGCCTGCGAACGCCAGATCGTCGAGGGCGCTGGCGCGGTCGGCATTGCAGCGTTGCTTG
CCGGGAAGATTGTGGGGAACGGCCCCATCGTCGCGATCCTGTCCGGGGCGAATGTCGACATGGAGCAGCATCGGCGGGTG
ATCAATGGCAAGGCGGCACTCTGCGGCGAGGAGAGACCATGA

Upstream 100 bases:

>100_bases
CTCGCGACCGCCTGGGCTTGCCTGCGGCTTTGCGGCGATGATCTGGCGCGGCCCGAACTCGGTCAATTGATGACCAAGCC
CTATCGGGAAGGCTGAGATC

Downstream 100 bases:

>100_bases
GCCGGATGATCATTCTGACGGAAGCGGAACTGAGGAAAGTCATCGCGCTTGATCGCGATACGGTTGATTGCGTCGAGGCC
GCTTTCGCAGCGCTTGCGAC

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MVSTLPVSLEDIRAAARRIAGRVIGTPMVQSFSLGELAGVPVHLKLEHHQTTGSFKLRGATNAVLSLSPAERSRGVVAAS
TGNHGRALAYAAKAEGAVATICMSRLVPDNKVSESRRLGADVRIVGGSQDEAQQEVERLVREEGLVMVPPFDHPDVVAGQ
GTLGLEIIDILPEAAIVLVPLSGGGLAAGVAAAVKGISPTTKVIGLTMEKGAAMKASLDAGRPVQVEEVSSLADSLGGGI
GLDNRVTFAMCRELLDDVILLTEVEIAVGMRHAYACERQIVEGAGAVGIAALLAGKIVGNGPIVAILSGANVDMEQHRRV
INGKAALCGEERP

Sequences:

>Translated_333_residues
MVSTLPVSLEDIRAAARRIAGRVIGTPMVQSFSLGELAGVPVHLKLEHHQTTGSFKLRGATNAVLSLSPAERSRGVVAAS
TGNHGRALAYAAKAEGAVATICMSRLVPDNKVSESRRLGADVRIVGGSQDEAQQEVERLVREEGLVMVPPFDHPDVVAGQ
GTLGLEIIDILPEAAIVLVPLSGGGLAAGVAAAVKGISPTTKVIGLTMEKGAAMKASLDAGRPVQVEEVSSLADSLGGGI
GLDNRVTFAMCRELLDDVILLTEVEIAVGMRHAYACERQIVEGAGAVGIAALLAGKIVGNGPIVAILSGANVDMEQHRRV
INGKAALCGEERP
>Mature_333_residues
MVSTLPVSLEDIRAAARRIAGRVIGTPMVQSFSLGELAGVPVHLKLEHHQTTGSFKLRGATNAVLSLSPAERSRGVVAAS
TGNHGRALAYAAKAEGAVATICMSRLVPDNKVSESRRLGADVRIVGGSQDEAQQEVERLVREEGLVMVPPFDHPDVVAGQ
GTLGLEIIDILPEAAIVLVPLSGGGLAAGVAAAVKGISPTTKVIGLTMEKGAAMKASLDAGRPVQVEEVSSLADSLGGGI
GLDNRVTFAMCRELLDDVILLTEVEIAVGMRHAYACERQIVEGAGAVGIAALLAGKIVGNGPIVAILSGANVDMEQHRRV
INGKAALCGEERP

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=323, Percent_Identity=34.6749226006192, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI19923959, Length=272, Percent_Identity=31.9852941176471, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI33469958, Length=316, Percent_Identity=26.8987341772152, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789505, Length=315, Percent_Identity=38.7301587301587, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1790207, Length=298, Percent_Identity=36.5771812080537, Blast_Score=154, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI71991565, Length=313, Percent_Identity=37.0607028753994, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17537387, Length=308, Percent_Identity=37.6623376623377, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17508781, Length=320, Percent_Identity=27.8125, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322631, Length=315, Percent_Identity=35.5555555555556, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320930, Length=332, Percent_Identity=34.6385542168675, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6319788, Length=249, Percent_Identity=28.1124497991968, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21355833, Length=310, Percent_Identity=39.0322580645161, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24645328, Length=214, Percent_Identity=38.3177570093458, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014333
- InterPro:   IPR001926
- InterPro:   IPR000634 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 34355; Mature: 34355

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSTLPVSLEDIRAAARRIAGRVIGTPMVQSFSLGELAGVPVHLKLEHHQTTGSFKLRGA
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCHHCCCCEEEEEECCCCCCCEEECCC
TNAVLSLSPAERSRGVVAASTGNHGRALAYAAKAEGAVATICMSRLVPDNKVSESRRLGA
CCEEEEECCCHHHCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHCCC
DVRIVGGSQDEAQQEVERLVREEGLVMVPPFDHPDVVAGQGTLGLEIIDILPEAAIVLVP
CEEEECCCCHHHHHHHHHHHHHCCEEEECCCCCCCCEECCCCCCHHHHHHCCCCEEEEEE
LSGGGLAAGVAAAVKGISPTTKVIGLTMEKGAAMKASLDAGRPVQVEEVSSLADSLGGGI
ECCCCHHHHHHHHHCCCCCCHHEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHCCCC
GLDNRVTFAMCRELLDDVILLTEVEIAVGMRHAYACERQIVEGAGAVGIAALLAGKIVGN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECC
GPIVAILSGANVDMEQHRRVINGKAALCGEERP
CCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MVSTLPVSLEDIRAAARRIAGRVIGTPMVQSFSLGELAGVPVHLKLEHHQTTGSFKLRGA
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCHHCCCCEEEEEECCCCCCCEEECCC
TNAVLSLSPAERSRGVVAASTGNHGRALAYAAKAEGAVATICMSRLVPDNKVSESRRLGA
CCEEEEECCCHHHCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHCCC
DVRIVGGSQDEAQQEVERLVREEGLVMVPPFDHPDVVAGQGTLGLEIIDILPEAAIVLVP
CEEEECCCCHHHHHHHHHHHHHCCEEEECCCCCCCCEECCCCCCHHHHHHCCCCEEEEEE
LSGGGLAAGVAAAVKGISPTTKVIGLTMEKGAAMKASLDAGRPVQVEEVSSLADSLGGGI
ECCCCHHHHHHHHHCCCCCCHHEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHCCCC
GLDNRVTFAMCRELLDDVILLTEVEIAVGMRHAYACERQIVEGAGAVGIAALLAGKIVGN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECC
GPIVAILSGANVDMEQHRRVINGKAALCGEERP
CCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]