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The map label for this gene is thuB [H]

Identifier: 116248716

GI number: 116248716

Start: 39232

End: 40269

Strand: Direct

Name: thuB [H]

Synonym: pRL120040

Alternate gene names: 116248716

Gene position: 39232-40269 (Clockwise)

Preceding gene: 116248715

Following gene: 116248722

Centisome position: 4.51

GC content: 58.38

Gene sequence:

>1038_bases
ATGCGCTTGATCATTCTCGGAACCGGAGGATGGGCAAACACCCATGCCATGAATTTTTCCGAAATCGCCGACGTAAAAAT
TGTTGCTGCTGTCGATACGGACGAGGTCCGGCTGCGGGCCTTCGCGCTCAGGCATGGTATCCCACTTACTTTCACGTCGC
TTGATGACGCCCTTGCCTGGGGAGAGTTTGACGCCGTGACCAATGTCACGCCGGATCGCGCGCATTATTCCACGACAATG
AAGATACTCGGCGCCGGCAAGCACGTCCTCTGCGAGAAGCCGCTGGCGGTTAACTACCGCGAAGCCAAAGAGATGGCCGA
CGCTGCCGCTGCGTCCGGCAAGGTCACGATGGTAAACCTTACCTATCGTAATGTAGCGCCGCTGCAAGCAGCGCGTAAGA
TGGTGCTGGACGGACGCCTCGGCGCGATCCGCCACTTCGAAGCGTCCTATCTCCAGAGCTGGCTGGTCTCAAAGGCGTGG
GGAGACTGGACCAAGGAATCGCAATGGCTTTGGCGGCTGTCGACAAAGCACGGCTCCAATGGCGTGCTGGGCGATGTCGG
TATCCATATTCTCGACTTCGCGGTTTTTGCCGCCGGAAGCGACGTCAAGGCGGCCGCATCGCATCTTAAGGTGTTCGACA
AGAGCCCCGGAAATCGGATCGGCGAGTATGATCTCGACGCTAACGACAGTTTCCTGATGATGGCTGAACTCGAAAACGGT
GCCGCTGGCGTCATCCACGCAACGCGCTGGGCGACTGGCCATCTCAACGAATTACGCCTGCGCCTGCATGGAGACAAGGG
CGCGCTGGAGGTGGTGCATACGCCTGAAGGTTCGACACTCAGGGCCTGCGAAGGTCCCGATGCCGACAAGGCAATCTGGC
GTAAGATCGAGGTCGAACCGGTAATCACCAATTTCCAGCGTTTTGCAAATGCCGTGCAAAAGGGGCAGCCGGATGAACCT
GGTTTCGGCCACGCAGCCAAGCTGCAATTCGTTCTCGACCACGCAGTGAAGACGGCCGGCGCTCTGACCGAACTTTAA

Upstream 100 bases:

>100_bases
CGCTGACAGTCGTCGCTCTCCATTTGCAGGACCATCTGGCGCTTGTGAAAGTCGACGGTTCTGGCGCTAATGATTGCGAT
AAACGACCATGAGGACAAAA

Downstream 100 bases:

>100_bases
GCGAAGGCGCTCCAGTTCGGTGCCACCTTTGCAGGTTGCTCGTCGTTTCAAGGCGAGGGCACCGGTTATGCGGCCTCCTT
GGAACCGGCGCTGACAGCCT

Product: putative GFO/IDH/MocA sugar uptake related protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MRLIILGTGGWANTHAMNFSEIADVKIVAAVDTDEVRLRAFALRHGIPLTFTSLDDALAWGEFDAVTNVTPDRAHYSTTM
KILGAGKHVLCEKPLAVNYREAKEMADAAAASGKVTMVNLTYRNVAPLQAARKMVLDGRLGAIRHFEASYLQSWLVSKAW
GDWTKESQWLWRLSTKHGSNGVLGDVGIHILDFAVFAAGSDVKAAASHLKVFDKSPGNRIGEYDLDANDSFLMMAELENG
AAGVIHATRWATGHLNELRLRLHGDKGALEVVHTPEGSTLRACEGPDADKAIWRKIEVEPVITNFQRFANAVQKGQPDEP
GFGHAAKLQFVLDHAVKTAGALTEL

Sequences:

>Translated_345_residues
MRLIILGTGGWANTHAMNFSEIADVKIVAAVDTDEVRLRAFALRHGIPLTFTSLDDALAWGEFDAVTNVTPDRAHYSTTM
KILGAGKHVLCEKPLAVNYREAKEMADAAAASGKVTMVNLTYRNVAPLQAARKMVLDGRLGAIRHFEASYLQSWLVSKAW
GDWTKESQWLWRLSTKHGSNGVLGDVGIHILDFAVFAAGSDVKAAASHLKVFDKSPGNRIGEYDLDANDSFLMMAELENG
AAGVIHATRWATGHLNELRLRLHGDKGALEVVHTPEGSTLRACEGPDADKAIWRKIEVEPVITNFQRFANAVQKGQPDEP
GFGHAAKLQFVLDHAVKTAGALTEL
>Mature_345_residues
MRLIILGTGGWANTHAMNFSEIADVKIVAAVDTDEVRLRAFALRHGIPLTFTSLDDALAWGEFDAVTNVTPDRAHYSTTM
KILGAGKHVLCEKPLAVNYREAKEMADAAAASGKVTMVNLTYRNVAPLQAARKMVLDGRLGAIRHFEASYLQSWLVSKAW
GDWTKESQWLWRLSTKHGSNGVLGDVGIHILDFAVFAAGSDVKAAASHLKVFDKSPGNRIGEYDLDANDSFLMMAELENG
AAGVIHATRWATGHLNELRLRLHGDKGALEVVHTPEGSTLRACEGPDADKAIWRKIEVEPVITNFQRFANAVQKGQPDEP
GFGHAAKLQFVLDHAVKTAGALTEL

Specific function: Unknown

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI1787574, Length=248, Percent_Identity=31.8548387096774, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24581117, Length=126, Percent_Identity=32.5396825396825, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 37586; Mature: 37586

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIILGTGGWANTHAMNFSEIADVKIVAAVDTDEVRLRAFALRHGIPLTFTSLDDALAW
CEEEEEECCCCCCCCCCCHHHHCCEEEEEEECCCHHHHHHHHHHCCCCEEEECCHHHHHC
GEFDAVTNVTPDRAHYSTTMKILGAGKHVLCEKPLAVNYREAKEMADAAAASGKVTMVNL
CCCCHHCCCCCCCHHHHHEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEE
TYRNVAPLQAARKMVLDGRLGAIRHFEASYLQSWLVSKAWGDWTKESQWLWRLSTKHGSN
EECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCC
GVLGDVGIHILDFAVFAAGSDVKAAASHLKVFDKSPGNRIGEYDLDANDSFLMMAELENG
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCC
AAGVIHATRWATGHLNELRLRLHGDKGALEVVHTPEGSTLRACEGPDADKAIWRKIEVEP
CEEEEEEEEECCCCCEEEEEEEECCCCCEEEEECCCCCEEEECCCCCCCHHHHHEEECCH
VITNFQRFANAVQKGQPDEPGFGHAAKLQFVLDHAVKTAGALTEL
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLIILGTGGWANTHAMNFSEIADVKIVAAVDTDEVRLRAFALRHGIPLTFTSLDDALAW
CEEEEEECCCCCCCCCCCHHHHCCEEEEEEECCCHHHHHHHHHHCCCCEEEECCHHHHHC
GEFDAVTNVTPDRAHYSTTMKILGAGKHVLCEKPLAVNYREAKEMADAAAASGKVTMVNL
CCCCHHCCCCCCCHHHHHEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEE
TYRNVAPLQAARKMVLDGRLGAIRHFEASYLQSWLVSKAWGDWTKESQWLWRLSTKHGSN
EECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCC
GVLGDVGIHILDFAVFAAGSDVKAAASHLKVFDKSPGNRIGEYDLDANDSFLMMAELENG
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCC
AAGVIHATRWATGHLNELRLRLHGDKGALEVVHTPEGSTLRACEGPDADKAIWRKIEVEP
CEEEEEEEEECCCCCEEEEEEEECCCCCEEEEECCCCCEEEECCCCCCCHHHHHEEECCH
VITNFQRFANAVQKGQPDEPGFGHAAKLQFVLDHAVKTAGALTEL
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]