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The map label for this gene is scrR [H]

Identifier: 116248698

GI number: 116248698

Start: 20570

End: 21604

Strand: Direct

Name: scrR [H]

Synonym: pRL120022

Alternate gene names: 116248698

Gene position: 20570-21604 (Clockwise)

Preceding gene: 116248693

Following gene: 116248701

Centisome position: 2.36

GC content: 60.0

Gene sequence:

>1035_bases
ATGGCCAAGAAGAATTATAAATCGATGGACGACTTCGCGACTGCCGCCGGCGTATCGCGTCCGACCTTGTCGAAATATTT
CGATGACCCCTCGCAGATCAAGGAGGTGACCCGCAAGCGTATCGAGGCGGCTCTCAGGAAGTCGCATTTCGAGCCGAATC
TTTTCGCGCGGCATCTCAATCGCAAGCGGACCAGGAACATCGGGATTCTGGTCCCGACCATGTCGGATCCCTTTTATGTG
CAGGTCGTGTCGCTGATCGAGCTGGAGTTGCGTGAAAAGGGATTCTGGCCTGTTCAGATTTCTTCGCACACGCGGCCGGA
GCTGGAGGCGGAGGCCGTTCGGACCTTGCTCTCGCTGAAGGTCGCGGGTGCGATCGTTGCGCCGCTCGGAGCCGGTTCGC
GCCGTTCCGCGCTGGAAAGGCTGAGGGATGCCATACCCGTCGTCTGCTTCGACAATCCTGCCGACGCCGACCTTCCTTAT
GTCGGCAACGACAACGCGCAAAGCATGGCAGCCATTGTGCAATATCTCTGCCGGTCCGGTGAGCCGCCGATCTTCCTGGA
AATTCCGCATATGACCGAGAATGCCGGCGAGCGGCAGGCGGGCTACCGCGCGACCATGGCGAGGGAAGGGCACTCCCCGC
TCGTCATCGATTGCGACGCCCCACCTTCCTGGGAATTCGAGCGTCTCGGATACGAGCAGATGCAGAGAATTCTTGCGAAT
GGCAGTCTTCCGGGCAAAACGCTGCTATGCGCCAACGACCGCTTTGCTTTCGGCGCAATGGCAGCCGCATTCGCGTCGGG
ACTGAAAATCGGCCGGAACGACGCGTGCGACGTGCGCATTGCCGGGCACGACGATCACCCGCTTAGCCGATATACCTGTC
CGTCGCTGACGACCATGGCCCAGAACGCGCCCGAGATCGCCGCAAAATCCGTGGAGCTTTTGCTGGCGCATATCGAGAGT
GAGGATGATGGAACGACCCCCGTGACGAACAAGGTAATCCTCGAGGCAACACTGGTTATGCGCGAGTCGGCTTGA

Upstream 100 bases:

>100_bases
TGTGCAAATTGCCGAATGCATGATTTGGCGCATCAGAGGATCGGCCGTTGAGTGAAATCGTTCCCGATCAGTTGTCGTCG
CCCCGCCAGTAAGGAATTGA

Downstream 100 bases:

>100_bases
GGCCGTGTTTCCGCGTTAACGGGCAAGCGCAGGGTCCATGGCGCGCCATGCTGTTGCGATGGCTTCGAGGGCCTCTTGCC
CCTCCGGAATGGCCCGTCTC

Product: putative HTH-type transcriptional regulator

Products: NA

Alternate protein names: Scr operon regulatory protein [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAKKNYKSMDDFATAAGVSRPTLSKYFDDPSQIKEVTRKRIEAALRKSHFEPNLFARHLNRKRTRNIGILVPTMSDPFYV
QVVSLIELELREKGFWPVQISSHTRPELEAEAVRTLLSLKVAGAIVAPLGAGSRRSALERLRDAIPVVCFDNPADADLPY
VGNDNAQSMAAIVQYLCRSGEPPIFLEIPHMTENAGERQAGYRATMAREGHSPLVIDCDAPPSWEFERLGYEQMQRILAN
GSLPGKTLLCANDRFAFGAMAAAFASGLKIGRNDACDVRIAGHDDHPLSRYTCPSLTTMAQNAPEIAAKSVELLLAHIES
EDDGTTPVTNKVILEATLVMRESA

Sequences:

>Translated_344_residues
MAKKNYKSMDDFATAAGVSRPTLSKYFDDPSQIKEVTRKRIEAALRKSHFEPNLFARHLNRKRTRNIGILVPTMSDPFYV
QVVSLIELELREKGFWPVQISSHTRPELEAEAVRTLLSLKVAGAIVAPLGAGSRRSALERLRDAIPVVCFDNPADADLPY
VGNDNAQSMAAIVQYLCRSGEPPIFLEIPHMTENAGERQAGYRATMAREGHSPLVIDCDAPPSWEFERLGYEQMQRILAN
GSLPGKTLLCANDRFAFGAMAAAFASGLKIGRNDACDVRIAGHDDHPLSRYTCPSLTTMAQNAPEIAAKSVELLLAHIES
EDDGTTPVTNKVILEATLVMRESA
>Mature_343_residues
AKKNYKSMDDFATAAGVSRPTLSKYFDDPSQIKEVTRKRIEAALRKSHFEPNLFARHLNRKRTRNIGILVPTMSDPFYVQ
VVSLIELELREKGFWPVQISSHTRPELEAEAVRTLLSLKVAGAIVAPLGAGSRRSALERLRDAIPVVCFDNPADADLPYV
GNDNAQSMAAIVQYLCRSGEPPIFLEIPHMTENAGERQAGYRATMAREGHSPLVIDCDAPPSWEFERLGYEQMQRILANG
SLPGKTLLCANDRFAFGAMAAAFASGLKIGRNDACDVRIAGHDDHPLSRYTCPSLTTMAQNAPEIAAKSVELLLAHIESE
DDGTTPVTNKVILEATLVMRESA

Specific function: Negative regulator of scrB expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=343, Percent_Identity=27.9883381924198, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790194, Length=338, Percent_Identity=25.4437869822485, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787948, Length=325, Percent_Identity=27.3846153846154, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=26.8436578171091, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI48994940, Length=321, Percent_Identity=25.2336448598131, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790715, Length=333, Percent_Identity=26.1261261261261, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789068, Length=327, Percent_Identity=24.4648318042813, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788474, Length=315, Percent_Identity=25.0793650793651, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790689, Length=338, Percent_Identity=23.0769230769231, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787906, Length=281, Percent_Identity=27.0462633451957, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789202, Length=300, Percent_Identity=24.6666666666667, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786268, Length=325, Percent_Identity=24, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37836; Mature: 37704

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKNYKSMDDFATAAGVSRPTLSKYFDDPSQIKEVTRKRIEAALRKSHFEPNLFARHLN
CCCCCCCCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RKRTRNIGILVPTMSDPFYVQVVSLIELELREKGFWPVQISSHTRPELEAEAVRTLLSLK
HHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHH
VAGAIVAPLGAGSRRSALERLRDAIPVVCFDNPADADLPYVGNDNAQSMAAIVQYLCRSG
HHHHHEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
EPPIFLEIPHMTENAGERQAGYRATMAREGHSPLVIDCDAPPSWEFERLGYEQMQRILAN
CCCEEEECCCCCCCCCCHHCCCHHHHHHCCCCCEEEECCCCCCCCHHHCCHHHHHHHHHC
GSLPGKTLLCANDRFAFGAMAAAFASGLKIGRNDACDVRIAGHDDHPLSRYTCPSLTTMA
CCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHH
QNAPEIAAKSVELLLAHIESEDDGTTPVTNKVILEATLVMRESA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKKNYKSMDDFATAAGVSRPTLSKYFDDPSQIKEVTRKRIEAALRKSHFEPNLFARHLN
CCCCCCCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RKRTRNIGILVPTMSDPFYVQVVSLIELELREKGFWPVQISSHTRPELEAEAVRTLLSLK
HHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHH
VAGAIVAPLGAGSRRSALERLRDAIPVVCFDNPADADLPYVGNDNAQSMAAIVQYLCRSG
HHHHHEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
EPPIFLEIPHMTENAGERQAGYRATMAREGHSPLVIDCDAPPSWEFERLGYEQMQRILAN
CCCEEEECCCCCCCCCCHHCCCHHHHHHCCCCCEEEECCCCCCCCHHHCCHHHHHHHHHC
GSLPGKTLLCANDRFAFGAMAAAFASGLKIGRNDACDVRIAGHDDHPLSRYTCPSLTTMA
CCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHH
QNAPEIAAKSVELLLAHIESEDDGTTPVTNKVILEATLVMRESA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA