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The map label for this gene is rbsK [H]

Identifier: 116248685

GI number: 116248685

Start: 8498

End: 9472

Strand: Direct

Name: rbsK [H]

Synonym: pRL120009

Alternate gene names: 116248685

Gene position: 8498-9472 (Clockwise)

Preceding gene: 116248684

Following gene: 116248686

Centisome position: 0.98

GC content: 63.49

Gene sequence:

>975_bases
TTGACGACTTCTGCTGCCTTCGGCCCACCTCCCGGTCGGGAGCGCCGCGTTTTGTGTGTCGGCGCGGCGGTTATGGACAC
CCTGTTTCGGGTGCGCACGTTGCCGACCGGCCAGGGCAAGATTCTGCCCTACGATATGCTCCAGATCGCCGAAGGCATGG
CATCGAGCGCCGCCTTTGCGGTCGTCCGGCTGGGCGGCGATGCCAGCCTCTGGGGCGCAGTCGGCAATGATGCCATCGGC
GAACGAATCGTTGCCGATCTCAGTGAGAGCGGCATAGACACGAGCGGCATGCTGCGTGTCGAAGGCGCCCGCTCGGCCAT
CTCGACCATTCTTGTCGATGATCAGGGCGAGCGCTTGATCGTGCCTTTCTACGATGCAGGCCTGCACGAAACGGTCAAGC
CGGTCACGGAACAGGACGCTTCTGCTTTCGATGCGGTGCTGGTCGACGTGCGATGGCCGAAGCTTGCACTGCGGACACTG
TTGGCGGCCCGGGAGGCGGGCAAGCCGGCCATTCTCGATGGCGATGTCGCAGGCGATGGTGTGATCGAGATGCTTGCACC
GGCGGCCAGCCACATCGTGTTCTCGCAGCCGGCCGCCGAGCGCCTTGCGGGTGCAGCCGAGCTGGTGAAGGTCGTCGGCC
TTCTGAAACGAAAGTTCGAGCATGCTTTCATCAGCGTCACGGCTGGCGAAAATGGCTCCTTCTGGTTCGATGACCTGACC
GGCGACGTCTTCCGTCTTGCCGCTCCGAAGGTGAGGGCGGTCGACACGCTCGCAGCCGGCGATGTCTTCCATGGCGCTTT
CGCGCTGGCTGTCGCCGAAGGCTTGCCGATCGAAGAAACGATGCGCTTTTCATCCATGGCGGCAGCGCTCAAATGCCAGG
TGTTCGGCGGACGCATTGGCGCACCCACCAGAGCCGAAGTCTGGGATGCCCTGCGGGATTGGGACGTGCCGATGACCAAG
ATGAGGGATCGTTGA

Upstream 100 bases:

>100_bases
CGTCACCTTGCCCGTGCTGATATTGACGGTGTTTGCGCAACGGCAGATCGTGGCGGGCCTGACGGCCGGAGCCGTCAAGT
GAGCGGGTGAGACAACGGTT

Downstream 100 bases:

>100_bases
TGGAAGAGGTCCGAAAGCCAATAGCCTAAGGCTGAAATGATCGCAGCGACGGGGAGTGTGACAACCCAGGCAACAACAAT
GTTGCCCGCCAAGCCCCAGC

Product: putative ribokinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MTTSAAFGPPPGRERRVLCVGAAVMDTLFRVRTLPTGQGKILPYDMLQIAEGMASSAAFAVVRLGGDASLWGAVGNDAIG
ERIVADLSESGIDTSGMLRVEGARSAISTILVDDQGERLIVPFYDAGLHETVKPVTEQDASAFDAVLVDVRWPKLALRTL
LAAREAGKPAILDGDVAGDGVIEMLAPAASHIVFSQPAAERLAGAAELVKVVGLLKRKFEHAFISVTAGENGSFWFDDLT
GDVFRLAAPKVRAVDTLAAGDVFHGAFALAVAEGLPIEETMRFSSMAAALKCQVFGGRIGAPTRAEVWDALRDWDVPMTK
MRDR

Sequences:

>Translated_324_residues
MTTSAAFGPPPGRERRVLCVGAAVMDTLFRVRTLPTGQGKILPYDMLQIAEGMASSAAFAVVRLGGDASLWGAVGNDAIG
ERIVADLSESGIDTSGMLRVEGARSAISTILVDDQGERLIVPFYDAGLHETVKPVTEQDASAFDAVLVDVRWPKLALRTL
LAAREAGKPAILDGDVAGDGVIEMLAPAASHIVFSQPAAERLAGAAELVKVVGLLKRKFEHAFISVTAGENGSFWFDDLT
GDVFRLAAPKVRAVDTLAAGDVFHGAFALAVAEGLPIEETMRFSSMAAALKCQVFGGRIGAPTRAEVWDALRDWDVPMTK
MRDR
>Mature_323_residues
TTSAAFGPPPGRERRVLCVGAAVMDTLFRVRTLPTGQGKILPYDMLQIAEGMASSAAFAVVRLGGDASLWGAVGNDAIGE
RIVADLSESGIDTSGMLRVEGARSAISTILVDDQGERLIVPFYDAGLHETVKPVTEQDASAFDAVLVDVRWPKLALRTLL
AAREAGKPAILDGDVAGDGVIEMLAPAASHIVFSQPAAERLAGAAELVKVVGLLKRKFEHAFISVTAGENGSFWFDDLTG
DVFRLAAPKVRAVDTLAAGDVFHGAFALAVAEGLPIEETMRFSSMAAALKCQVFGGRIGAPTRAEVWDALRDWDVPMTKM
RDR

Specific function: Unknown

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI87082343, Length=287, Percent_Identity=37.9790940766551, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1790193, Length=320, Percent_Identity=26.25, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17532905, Length=314, Percent_Identity=28.0254777070064, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR002139 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: NA

Molecular weight: Translated: 34350; Mature: 34219

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSAAFGPPPGRERRVLCVGAAVMDTLFRVRTLPTGQGKILPYDMLQIAEGMASSAAFA
CCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEE
VVRLGGDASLWGAVGNDAIGERIVADLSESGIDTSGMLRVEGARSAISTILVDDQGERLI
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHEEEECCCCCEEE
VPFYDAGLHETVKPVTEQDASAFDAVLVDVRWPKLALRTLLAAREAGKPAILDGDVAGDG
EEECCCCCHHHHHHHHHCCHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCEEECCCCCCH
VIEMLAPAASHIVFSQPAAERLAGAAELVKVVGLLKRKFEHAFISVTAGENGSFWFDDLT
HHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCEEEECCC
GDVFRLAAPKVRAVDTLAAGDVFHGAFALAVAEGLPIEETMRFSSMAAALKCQVFGGRIG
CCHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCC
APTRAEVWDALRDWDVPMTKMRDR
CCCHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure 
TTSAAFGPPPGRERRVLCVGAAVMDTLFRVRTLPTGQGKILPYDMLQIAEGMASSAAFA
CCCCCCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEE
VVRLGGDASLWGAVGNDAIGERIVADLSESGIDTSGMLRVEGARSAISTILVDDQGERLI
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHEEEECCCCCEEE
VPFYDAGLHETVKPVTEQDASAFDAVLVDVRWPKLALRTLLAAREAGKPAILDGDVAGDG
EEECCCCCHHHHHHHHHCCHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCEEECCCCCCH
VIEMLAPAASHIVFSQPAAERLAGAAELVKVVGLLKRKFEHAFISVTAGENGSFWFDDLT
HHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCEEEECCC
GDVFRLAAPKVRAVDTLAAGDVFHGAFALAVAEGLPIEETMRFSSMAAALKCQVFGGRIG
CCHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCC
APTRAEVWDALRDWDVPMTKMRDR
CCCHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8346018; 9278503 [H]