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The map label for this gene is metH [H]

Identifier: 115353017

GI number: 115353017

Start: 3269504

End: 3272221

Strand: Direct

Name: metH [H]

Synonym: Bamb_2966

Alternate gene names: 115353017

Gene position: 3269504-3272221 (Clockwise)

Preceding gene: 115353016

Following gene: 115353019

Centisome position: 91.93

GC content: 65.16

Gene sequence:

>2718_bases
ATGACCGATCACATGATGCGCCTTGCCGGCCTCGAGCCGTTCAACGTCACGTCCGGGACGCTCTTCATCAACGTCGGCGA
ACGCACCAACGTCACCGGCTCGAAGGCGTTCGCGCGGATGATCCTGAACGGCCAGTTCGACGAGGCGCTCGCCGTCGCGC
GCCAGCAGGTCGAGAACGGCGCGCAGGTGATCGACATCAACATGGACGAAGCGATGCTCGATTCGAAGGCGGCGATGGTG
CGCTTCCTGAACCTGATCGCATCCGAGCCGGACATCGCGCGCGTGCCGATCATGATCGACTCGTCGAAGTGGGAAGTGAT
CGAGGCCGGCCTCAAGTGCGTGCAGGGCAAGGCGATCGTGAACTCGATCTCGCTGAAGGAAGGCGAGGAAGCGTTCCGCC
ATCACGCGAACCTGATCCGCCGCTACGGCGCGGCCGCGGTCGTGATGGCGTTCGACGAGACGGGCCAGGCCGATACCTAC
GCGCGCAAGACCGAGATCTGCAAGCGCTCGTACGACTTTCTCGTGAACGAAGTCGGCTTCCCGCCGGAAGACATCATCTT
CGACCCGAACATCTTCGCGGTCGCGACCGGCATCGAGGAGCACAACAACTACGCGGTCGACTTCATCGAGGCGACCCGCT
GGATCAAGCAGAACCTGCCGTACGCGAAGGTGAGCGGCGGCGTGTCGAACGTGTCGTTCTCGTTCCGCGGCAACGACCCG
GTGCGCGAGGCGATCCACACCGTGTTCCTGTACCACGCGATCCAGGCGGGGATGGACATGGGCATCGTGAACGCGGGCCA
GCTCGGCGTGTACGCCGATCTCGACGCGGAACTGCGCGAGCGGGTCGAGGACGTGATCCTGAACCGCCGTGACGATTCCA
CCGACCGCCTGCTCGAAATCGCCGACAAGTTCAAGGCGGGCGGCGCGAAGAAGGAAGAGAACCTCGAGTGGCGCAATCAG
CCGGTCGAGAAGCGGCTTTCGCATGCGCTCGTGCACGGCATCACGAACTTCATCGTCGAGGATACGGAAGAGGTGCGCGC
GAAGATCGCCGCTGCCGGCGGCCGCCCGATCAACGTGATCGAAGGCCCGCTGATGGACGGGATGAACATCGTCGGCGACC
TGTTCGGCCAGGGCAAGATGTTCCTGCCGCAGGTCGTGAAGTCGGCGCGCGTGATGAAGCAGGCCGTCGCGCACCTGATC
CCGTTCATCGAGGAAGAAAAACGGCTGCTCGCCGAAGCGGGCGGCGACGTGCGCGCGAAGGGCAAGATCGTCATCGCGAC
CGTGAAGGGCGACGTGCATGACATCGGCAAGAACATCGTGTCGGTCGTGCTCCAGTGCAACAACTTCGAAGTCGTCAACA
TGGGCGTGATGGTCCCGTGCAACGAGATCCTCGCGAAGGCGAAGGTCGAGGGCGCGGACATCATCGGGCTGTCTGGCCTC
ATCACGCCGAGCCTCGAGGAAATGGCGTACGTCGCGTCCGAAATGCAGCGCGACGACTACTTCCGCGTGAAGAAGATTCC
GCTCCTGATCGGCGGCGCGACGACCTCGCGCGTGCACACGGCCGTGAAGATCGCGCCGCATTACGAAGGCCCGGTCGTCT
ACGTGCCCGACGCATCGCGCTCGGTGTCGGTCGCATCGAGCCTGCTGTCGGACGAAGGCGCGACGAAGTACCTCGACGAA
TTGAAGTCGGATTACGAACGGATCCGCGAACAGCACGCGAACCGCAAGGCCCAGCCGATGGTCACGCTCGCCGAGGCGCG
CGCGAACAAGGCGAAGCTCGACTGGGCGGGCTACCGGCCCGTGAAGCCGAAGTTCATCGGCCGCCGCGTGTTCAAGAACT
ACGACCTGAACGAGCTCGCGAACTACATCGACTGGGGCCCGTTCTTCCAGACCTGGGATCTCGCGGGCCCGTACCCGGCG
ATCCTGAACGACGAGATCGTCGGCGAATCGGCGCGGCGCGTGTTCTCCGACGCGAAGTCGATGCTCGCCCGCCTGATCCA
GGGCCGCTGGCTCACCGCGAACGGCGTGATCTCGCTGCTGCCGGCGAACACGGTCAACGACGACGACATCGAGATCTACA
CCGACGATTCGCGCTCCAAAGTGCTGCTCACGTGGCGCAACCTGCGCCAGCAGAGCGTGCGTCCGGTGGTCGACGGCGTG
ATGCGCCCGAACCGCTCGCTCGCCGACTTCATCGCGCCGAAGGAATCGGGCGTCGCCGACTACATCGGGATGTTCGCGGT
CACGGCCGGCCTCGGCGTCGACGTGAAGGAAAAGCAGTTCGAGGCCGACCACGACGACTACAGCGCGATCATGCTGAAGG
CGCTCGCCGACCGCTTCGCGGAAGCATTCGCCGAGGCGATGCACGCGCGCGTGCGCCGCGAGCTGTGGGGCTATGCGAGC
GGCGAGACGCTCGACAACGATGCGCTGATCGCCGAAAAGTACGCGGGCATCCGCCCGGCGCCCGGCTACCCGGCCTGCCC
CGACCACCTCGTGAAGCGCGACATGTTCGCCGCGCTGCATGCGGAAGAGATCGGAATGAGCGTGACCGAATCGCTGGCGA
TGCTGCCGGCGGCGAGCGTGTCGGGCTTCTATCTCGCGCATCCGGACAGCCGCTATTTCTCGGTCGGCAAAATCGGGCAG
GATCAGCTCGACGACTACGCGCGGCGGATGTCGCTGTCGCTCGCCGACGCCCGGCGCGCGCTCGCGCCGCAGCTCTGA

Upstream 100 bases:

>100_bases
CTCGCCGACGTGAAACCGCGCCGCTGGCCGAGCCACTACAGCGACGCCGCCTGACCGACCGCCCCAACGCCCGCCCTACC
TCACACGAATTCGCACCGCC

Downstream 100 bases:

>100_bases
CCGATGCCGGCGGGCGGGCGGCCCGCCATTTCGTCAGAGGAAATGAAAAGCCCGCTCGAAAGCGGGCTTTTTTTGCGTTG
CCGACGATCTTTCGATCGGG

Product: methionine synthase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 905; Mature: 904

Protein sequence:

>905_residues
MTDHMMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMV
RFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEEAFRHHANLIRRYGAAAVVMAFDETGQADTY
ARKTEICKRSYDFLVNEVGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDAELRERVEDVILNRRDDSTDRLLEIADKFKAGGAKKEENLEWRNQ
PVEKRLSHALVHGITNFIVEDTEEVRAKIAAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLI
PFIEEEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASSLLSDEGATKYLDE
LKSDYERIREQHANRKAQPMVTLAEARANKAKLDWAGYRPVKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPA
ILNDEIVGESARRVFSDAKSMLARLIQGRWLTANGVISLLPANTVNDDDIEIYTDDSRSKVLLTWRNLRQQSVRPVVDGV
MRPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFAEAMHARVRRELWGYAS
GETLDNDALIAEKYAGIRPAPGYPACPDHLVKRDMFAALHAEEIGMSVTESLAMLPAASVSGFYLAHPDSRYFSVGKIGQ
DQLDDYARRMSLSLADARRALAPQL

Sequences:

>Translated_905_residues
MTDHMMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMV
RFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEEAFRHHANLIRRYGAAAVVMAFDETGQADTY
ARKTEICKRSYDFLVNEVGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDAELRERVEDVILNRRDDSTDRLLEIADKFKAGGAKKEENLEWRNQ
PVEKRLSHALVHGITNFIVEDTEEVRAKIAAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLI
PFIEEEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASSLLSDEGATKYLDE
LKSDYERIREQHANRKAQPMVTLAEARANKAKLDWAGYRPVKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPA
ILNDEIVGESARRVFSDAKSMLARLIQGRWLTANGVISLLPANTVNDDDIEIYTDDSRSKVLLTWRNLRQQSVRPVVDGV
MRPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFAEAMHARVRRELWGYAS
GETLDNDALIAEKYAGIRPAPGYPACPDHLVKRDMFAALHAEEIGMSVTESLAMLPAASVSGFYLAHPDSRYFSVGKIGQ
DQLDDYARRMSLSLADARRALAPQL
>Mature_904_residues
TDHMMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMVR
FLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEEAFRHHANLIRRYGAAAVVMAFDETGQADTYA
RKTEICKRSYDFLVNEVGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDPV
REAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDAELRERVEDVILNRRDDSTDRLLEIADKFKAGGAKKEENLEWRNQP
VEKRLSHALVHGITNFIVEDTEEVRAKIAAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIP
FIEEEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGLI
TPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASSLLSDEGATKYLDEL
KSDYERIREQHANRKAQPMVTLAEARANKAKLDWAGYRPVKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPAI
LNDEIVGESARRVFSDAKSMLARLIQGRWLTANGVISLLPANTVNDDDIEIYTDDSRSKVLLTWRNLRQQSVRPVVDGVM
RPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFAEAMHARVRRELWGYASG
ETLDNDALIAEKYAGIRPAPGYPACPDHLVKRDMFAALHAEEIGMSVTESLAMLPAASVSGFYLAHPDSRYFSVGKIGQD
QLDDYARRMSLSLADARRALAPQL

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=917, Percent_Identity=54.525627044711, Blast_Score=974, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=904, Percent_Identity=62.2787610619469, Blast_Score=1102, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=912, Percent_Identity=54.7149122807018, Blast_Score=949, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 99948; Mature: 99817

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHMMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENG
CCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHH
NSISLKEGEEAFRHHANLIRRYGAAAVVMAFDETGQADTYARKTEICKRSYDFLVNEVGF
HHCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
CCHHEEECCCCEEEEECCHHCCCEEEHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCH
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDAELRERVEDVILNRRDDSTDRLLEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHH
ADKFKAGGAKKEENLEWRNQPVEKRLSHALVHGITNFIVEDTEEVRAKIAAAGGRPINVI
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEE
EGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLLAEAGGDVRAK
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
CEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEEEECCC
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASR
CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHHEEEEEEECCCCCCEEECCCCCC
SVSVASSLLSDEGATKYLDELKSDYERIREQHANRKAQPMVTLAEARANKAKLDWAGYRP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCC
VKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDAKS
CCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
MLARLIQGRWLTANGVISLLPANTVNDDDIEIYTDDSRSKVLLTWRNLRQQSVRPVVDGV
HHHHHHCCCEEECCCEEEEECCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHH
MRPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFA
HCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
EAFAEAMHARVRRELWGYASGETLDNDALIAEKYAGIRPAPGYPACPDHLVKRDMFAALH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
AEEIGMSVTESLAMLPAASVSGFYLAHPDSRYFSVGKIGQDQLDDYARRMSLSLADARRA
HHHHCHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LAPQL
HCCCC
>Mature Secondary Structure 
TDHMMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENG
CCHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHH
NSISLKEGEEAFRHHANLIRRYGAAAVVMAFDETGQADTYARKTEICKRSYDFLVNEVGF
HHCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
CCHHEEECCCCEEEEECCHHCCCEEEHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCH
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDAELRERVEDVILNRRDDSTDRLLEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHH
ADKFKAGGAKKEENLEWRNQPVEKRLSHALVHGITNFIVEDTEEVRAKIAAAGGRPINVI
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEE
EGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLLAEAGGDVRAK
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
CEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEEEECCC
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASR
CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHHEEEEEEECCCCCCEEECCCCCC
SVSVASSLLSDEGATKYLDELKSDYERIREQHANRKAQPMVTLAEARANKAKLDWAGYRP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCC
VKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDAKS
CCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
MLARLIQGRWLTANGVISLLPANTVNDDDIEIYTDDSRSKVLLTWRNLRQQSVRPVVDGV
HHHHHHCCCEEECCCEEEEECCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHH
MRPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFA
HCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
EAFAEAMHARVRRELWGYASGETLDNDALIAEKYAGIRPAPGYPACPDHLVKRDMFAALH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
AEEIGMSVTESLAMLPAASVSGFYLAHPDSRYFSVGKIGQDQLDDYARRMSLSLADARRA
HHHHCHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LAPQL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]