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The map label for this gene is metH [H]

Identifier: 115353016

GI number: 115353016

Start: 3268390

End: 3269457

Strand: Direct

Name: metH [H]

Synonym: Bamb_2965

Alternate gene names: 115353016

Gene position: 3268390-3269457 (Clockwise)

Preceding gene: 115353015

Following gene: 115353017

Centisome position: 91.9

GC content: 67.79

Gene sequence:

>1068_bases
ATGTCTGCGACCCCTCTCGCTGCTTCCGTCCCGCTCGACGCGCGCTACACGCGCGGCGCCGAACTGCCCGCGCTGCTGAA
ATCGCGCATCCTGATCCTCGACGGCGCGATGGGCACGATGATCCAGCGCTACAAACTCGACGAGGCCGCCTATCGCGGCG
AGCGCTTCAAGGATTTCCCGCGCGACATCAAGGGCAACAACGAGCTGCTGTCGCTCACGCAGCCGCAGATCATCCGCGAG
ATCCACGACCAGTACTTCGCGGCCGGCGCGGACATCGTCGAAACCAACACGTTCGGCGCGACGACGGTCGCGCAGGCCGA
CTACGGGATGGAAGACCTCGTCGTCGAGATGAACGTCGAATCGGCGCGGCTCGCGCGCGAATCGGCCGTGCGCTACGCGA
CGCCGGACAAACCGCGCTTCGTCGCGGGCGCGATCGGGCCGACGCCGAAGACGGCGAGCATCTCGCCGGACGTCAACGAC
CCGGGCGCGCGCAACGTCACGTTTGACGAACTGCGCAGCGCGTATTACCAGCAGGCGAAGGCGCTACTCGACGGCGGCGT
CGACCTGTTCCTCGTCGAGACGATCTTCGACACGCTGAACGCGAAGGCCGCGCTGTTCGCGCTCGACGAATTGTTCGAGG
ACACCGGCGAGCGCCTGCCGATCATGATCTCGGGCACCGTGACCGACGCGTCGGGCCGCATCCTGTCGGGCCAGACGGTC
GAGGCGTTCTGGAATTCGCTGCGCCACGCGAAGCCGCTCACGTTCGGCCTGAACTGCGCGCTCGGCGCGGCGCTGATGCG
CCCGTACATCGCCGAACTCGCGAAGCTGTGCGACACCTACGTGTCGTGCTACCCGAACGCGGGCCTGCCGAACCCGATGA
GCGACACCGGTTTCGACGAGACGCCGGACGTCACGTCGGGCCTGCTGAAGGAATTCGCGCAAGCCGGGCTCGTCAACCTC
GCGGGCGGCTGCTGCGGCACGACGCCCGAGCACATCGCCGAGATCGCGAAGGCGCTCGCCGACGTGAAACCGCGCCGCTG
GCCGAGCCACTACAGCGACGCCGCCTGA

Upstream 100 bases:

>100_bases
CGCGTCGCGCCGGCCCCGCTGCCCGCGCCGCGCGAGCCCGGGCGGCGCCCCGGCGCACCGGCTACAATGACGGTTTTCCC
GTCTTCGCCGGTCTTCCGCC

Downstream 100 bases:

>100_bases
CCGACCGCCCCAACGCCCGCCCTACCTCACACGAATTCGCACCGCCATGACCGATCACATGATGCGCCTTGCCGGCCTCG
AGCCGTTCAACGTCACGTCC

Product: methionine synthase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSATPLAASVPLDARYTRGAELPALLKSRILILDGAMGTMIQRYKLDEAAYRGERFKDFPRDIKGNNELLSLTQPQIIRE
IHDQYFAAGADIVETNTFGATTVAQADYGMEDLVVEMNVESARLARESAVRYATPDKPRFVAGAIGPTPKTASISPDVND
PGARNVTFDELRSAYYQQAKALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMISGTVTDASGRILSGQTV
EAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDETPDVTSGLLKEFAQAGLVNL
AGGCCGTTPEHIAEIAKALADVKPRRWPSHYSDAA

Sequences:

>Translated_355_residues
MSATPLAASVPLDARYTRGAELPALLKSRILILDGAMGTMIQRYKLDEAAYRGERFKDFPRDIKGNNELLSLTQPQIIRE
IHDQYFAAGADIVETNTFGATTVAQADYGMEDLVVEMNVESARLARESAVRYATPDKPRFVAGAIGPTPKTASISPDVND
PGARNVTFDELRSAYYQQAKALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMISGTVTDASGRILSGQTV
EAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDETPDVTSGLLKEFAQAGLVNL
AGGCCGTTPEHIAEIAKALADVKPRRWPSHYSDAA
>Mature_354_residues
SATPLAASVPLDARYTRGAELPALLKSRILILDGAMGTMIQRYKLDEAAYRGERFKDFPRDIKGNNELLSLTQPQIIREI
HDQYFAAGADIVETNTFGATTVAQADYGMEDLVVEMNVESARLARESAVRYATPDKPRFVAGAIGPTPKTASISPDVNDP
GARNVTFDELRSAYYQQAKALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMISGTVTDASGRILSGQTVE
AFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDETPDVTSGLLKEFAQAGLVNLA
GGCCGTTPEHIAEIAKALADVKPRRWPSHYSDAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=343, Percent_Identity=58.0174927113703, Blast_Score=394, Evalue=1e-110,
Organism=Escherichia coli, GI1790450, Length=330, Percent_Identity=58.7878787878788, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1786456, Length=325, Percent_Identity=27.0769230769231, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17535405, Length=331, Percent_Identity=54.6827794561934, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38349; Mature: 38218

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATPLAASVPLDARYTRGAELPALLKSRILILDGAMGTMIQRYKLDEAAYRGERFKDFP
CCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHCC
RDIKGNNELLSLTQPQIIREIHDQYFAAGADIVETNTFGATTVAQADYGMEDLVVEMNVE
HHCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCCCHHHEEEEECCH
SARLARESAVRYATPDKPRFVAGAIGPTPKTASISPDVNDPGARNVTFDELRSAYYQQAK
HHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMISGTVTDASGRILSGQTV
HHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEECCCHH
EAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDE
HHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
TPDVTSGLLKEFAQAGLVNLAGGCCGTTPEHIAEIAKALADVKPRRWPSHYSDAA
CCHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SATPLAASVPLDARYTRGAELPALLKSRILILDGAMGTMIQRYKLDEAAYRGERFKDFP
CCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHCC
RDIKGNNELLSLTQPQIIREIHDQYFAAGADIVETNTFGATTVAQADYGMEDLVVEMNVE
HHCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCCCHHHEEEEECCH
SARLARESAVRYATPDKPRFVAGAIGPTPKTASISPDVNDPGARNVTFDELRSAYYQQAK
HHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALLDGGVDLFLVETIFDTLNAKAALFALDELFEDTGERLPIMISGTVTDASGRILSGQTV
HHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEECCCHH
EAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDE
HHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
TPDVTSGLLKEFAQAGLVNLAGGCCGTTPEHIAEIAKALADVKPRRWPSHYSDAA
CCHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]