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The map label for this gene is dacB [H]

Identifier: 115352717

GI number: 115352717

Start: 2936201

End: 2937817

Strand: Direct

Name: dacB [H]

Synonym: Bamb_2666

Alternate gene names: 115352717

Gene position: 2936201-2937817 (Clockwise)

Preceding gene: 115352710

Following gene: 115352718

Centisome position: 82.56

GC content: 70.32

Gene sequence:

>1617_bases
GTGCGCACTTGTCTCTACAATGTGCGCTCCGGCGCGCCGCGCGCCCCGCCCGAACCCCTGCATTTCCCGTATTCGCCCAT
GCCGATTTCCGATCTCTCCGCCCGGTTCGCCCCCCGCGCCCGCGCCCGCGTCTGCCTGCGGGCGGCCGCCGTCGTCGCCA
CCTGCACGGCCCTTGCGTTCGCACCTTCCGCGCAGGCCCGCAAGAAGCTTCACAAGGAAGCGCGCAAGACCGCCGTCGTG
TCGCCCGCCGCGCGCGCGGCCGGCCTGCCGGCGTCCGTGCTGGTCGCGCTGCAGCGCGCGAAGGTGCCGGCATCGGCGAT
GAGCGTCGTCGTCGAGCGCGTCGGCGATCCCGAGCCGCTGATCGAATGGAACGCGAACCGGCCGATGCTGCCGGCGTCGA
CGATGAAGCTCGTCACGACCTTCTCGGGCCTGTCGATCCTCGGCCCCGACTTCCGCTGGCGCACCACCGCGTATGCGGAC
GGCCCGGTCGATCCGGACGGCACGCTGCAAGGCAACCTGTACATCAAGGGCACCGGCGATCCGAAGCTCGTGCCCGAGGA
GCTGATCGACCTCGTCGACAAGCTCCGCAAGGCGGGCGTCAAGCGCGTGGCCGGCGGCCTCGTGCTCGACAAGAGCTATT
TCGCCGCGTCGACGCGCGACCTGCCGTCGTTCGACGACGACGTGAGCGCACCGTACAACGTCGGCCCCGATCCGCTGCTG
TACGCGTTCAAGGCCGTGTCGTTCACGGTGACGCCGGGCGACGACGGCAAGATCGCCGTCGATGTGCTGCCGCCGCTCGC
CAATCTGTCGATCGACAACCAGCTGTACGAAGGCAGCGGATCGTGCGGCGCGGCCGCCGCGGCCGCGCGTCCGACGCTGA
CGGCCGGCAGCGGGATCATGACCGCGTCGTTCGCCGGCGACTATCCGCTGCGCTGCGGCGCGCGCACGACCAATCTCGCG
ATCCTCGATCACACGACGTTCTTCGCGCGCGGCTTCCTCGCGCTGTGGCAGCAGGACGGCGGCACGATCGCGGGCCCGGT
CAGCGAAGGCAAGGTGCCCACCACCGCGCGCCCGCTCGCCGTCCATCACAGCCCGGTGCTCGGCAGCATCGTCTACGACA
TCAACAAGTTCAGCAACAACGTGATGGCGCGCAACCTGTTCCTGACGATCGGCGCGGTCGCCGGCAAGCCGCCTGCGACA
CCCGAGCAGTCGAGCCGCGCGATTCAGGCATTCCTGCGCAAGAGCGGGATCGCGATGCCCGACCTCGCGCTCGAGAACGG
CTCGGGCCTGTCGCGCGACGAGCACGTGAGCGCGCTGTCGCTCGCCGCGCTGCTGCAGGCCGCGAACGCGAGCCCGGTCG
CGCAGGCGTTCGTCGATTCGCTGCCGGTCGCCGGCATCGACGGCACGATGAAGAACCGCCTGACCAACGCCGGCGTGCTC
GGCAATGCGCACATCAAGACGGGCACGCTGCGCGACGTGCGCGCGATCGCGGGCTACGTCGCGGGCGCGGACGGCCAGAG
CTACGTCGTCGTCAGTTTCATCAACGACGATCACGCGGAAGCCGCGCGCGCCGCGCACGACACGCTGCTCGAATGGATCT
ACGCGGGCGCGCACTGA

Upstream 100 bases:

>100_bases
AACCGGAACCCATGGGGCGAGTCAACATGGCGACCATTGTAGCGCCGCGAATCGCGCGCGCCGCCTGCAATGCAACAGAA
AGGCGGATTGCAAGGAGCGT

Downstream 100 bases:

>100_bases
CGGGACGACGCGCGCCGCGACGCGGCCCCCCCACGCACCGCCGGCGCATCGCGCCACAAAACGGGGCCGCACCGCGCGGC
CCCGTTGCACATGGTTTTCC

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MRTCLYNVRSGAPRAPPEPLHFPYSPMPISDLSARFAPRARARVCLRAAAVVATCTALAFAPSAQARKKLHKEARKTAVV
SPAARAAGLPASVLVALQRAKVPASAMSVVVERVGDPEPLIEWNANRPMLPASTMKLVTTFSGLSILGPDFRWRTTAYAD
GPVDPDGTLQGNLYIKGTGDPKLVPEELIDLVDKLRKAGVKRVAGGLVLDKSYFAASTRDLPSFDDDVSAPYNVGPDPLL
YAFKAVSFTVTPGDDGKIAVDVLPPLANLSIDNQLYEGSGSCGAAAAAARPTLTAGSGIMTASFAGDYPLRCGARTTNLA
ILDHTTFFARGFLALWQQDGGTIAGPVSEGKVPTTARPLAVHHSPVLGSIVYDINKFSNNVMARNLFLTIGAVAGKPPAT
PEQSSRAIQAFLRKSGIAMPDLALENGSGLSRDEHVSALSLAALLQAANASPVAQAFVDSLPVAGIDGTMKNRLTNAGVL
GNAHIKTGTLRDVRAIAGYVAGADGQSYVVVSFINDDHAEAARAAHDTLLEWIYAGAH

Sequences:

>Translated_538_residues
MRTCLYNVRSGAPRAPPEPLHFPYSPMPISDLSARFAPRARARVCLRAAAVVATCTALAFAPSAQARKKLHKEARKTAVV
SPAARAAGLPASVLVALQRAKVPASAMSVVVERVGDPEPLIEWNANRPMLPASTMKLVTTFSGLSILGPDFRWRTTAYAD
GPVDPDGTLQGNLYIKGTGDPKLVPEELIDLVDKLRKAGVKRVAGGLVLDKSYFAASTRDLPSFDDDVSAPYNVGPDPLL
YAFKAVSFTVTPGDDGKIAVDVLPPLANLSIDNQLYEGSGSCGAAAAAARPTLTAGSGIMTASFAGDYPLRCGARTTNLA
ILDHTTFFARGFLALWQQDGGTIAGPVSEGKVPTTARPLAVHHSPVLGSIVYDINKFSNNVMARNLFLTIGAVAGKPPAT
PEQSSRAIQAFLRKSGIAMPDLALENGSGLSRDEHVSALSLAALLQAANASPVAQAFVDSLPVAGIDGTMKNRLTNAGVL
GNAHIKTGTLRDVRAIAGYVAGADGQSYVVVSFINDDHAEAARAAHDTLLEWIYAGAH
>Mature_538_residues
MRTCLYNVRSGAPRAPPEPLHFPYSPMPISDLSARFAPRARARVCLRAAAVVATCTALAFAPSAQARKKLHKEARKTAVV
SPAARAAGLPASVLVALQRAKVPASAMSVVVERVGDPEPLIEWNANRPMLPASTMKLVTTFSGLSILGPDFRWRTTAYAD
GPVDPDGTLQGNLYIKGTGDPKLVPEELIDLVDKLRKAGVKRVAGGLVLDKSYFAASTRDLPSFDDDVSAPYNVGPDPLL
YAFKAVSFTVTPGDDGKIAVDVLPPLANLSIDNQLYEGSGSCGAAAAAARPTLTAGSGIMTASFAGDYPLRCGARTTNLA
ILDHTTFFARGFLALWQQDGGTIAGPVSEGKVPTTARPLAVHHSPVLGSIVYDINKFSNNVMARNLFLTIGAVAGKPPAT
PEQSSRAIQAFLRKSGIAMPDLALENGSGLSRDEHVSALSLAALLQAANASPVAQAFVDSLPVAGIDGTMKNRLTNAGVL
GNAHIKTGTLRDVRAIAGYVAGADGQSYVVVSFINDDHAEAARAAHDTLLEWIYAGAH

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=451, Percent_Identity=27.2727272727273, Blast_Score=142, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 56376; Mature: 56376

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTCLYNVRSGAPRAPPEPLHFPYSPMPISDLSARFAPRARARVCLRAAAVVATCTALAF
CCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
APSAQARKKLHKEARKTAVVSPAARAAGLPASVLVALQRAKVPASAMSVVVERVGDPEPL
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
IEWNANRPMLPASTMKLVTTFSGLSILGPDFRWRTTAYADGPVDPDGTLQGNLYIKGTGD
EEECCCCCCCCHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCCCCCEEEEEEEEECCC
PKLVPEELIDLVDKLRKAGVKRVAGGLVLDKSYFAASTRDLPSFDDDVSAPYNVGPDPLL
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
YAFKAVSFTVTPGDDGKIAVDVLPPLANLSIDNQLYEGSGSCGAAAAAARPTLTAGSGIM
HEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCEECCCCCCCCHHHHCCCCEECCCCEE
TASFAGDYPLRCGARTTNLAILDHTTFFARGFLALWQQDGGTIAGPVSEGKVPTTARPLA
EEECCCCCCEECCCCCCEEEEEECHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEE
VHHSPVLGSIVYDINKFSNNVMARNLFLTIGAVAGKPPATPEQSSRAIQAFLRKSGIAMP
EECCCHHHHHHHHHHHHCCCHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCC
DLALENGSGLSRDEHVSALSLAALLQAANASPVAQAFVDSLPVAGIDGTMKNRLTNAGVL
CEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCEE
GNAHIKTGTLRDVRAIAGYVAGADGQSYVVVSFINDDHAEAARAAHDTLLEWIYAGAH
CCCEEECCCHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTCLYNVRSGAPRAPPEPLHFPYSPMPISDLSARFAPRARARVCLRAAAVVATCTALAF
CCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
APSAQARKKLHKEARKTAVVSPAARAAGLPASVLVALQRAKVPASAMSVVVERVGDPEPL
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
IEWNANRPMLPASTMKLVTTFSGLSILGPDFRWRTTAYADGPVDPDGTLQGNLYIKGTGD
EEECCCCCCCCHHHHHHHHHHCCCEEECCCCCEEEEEECCCCCCCCCCEEEEEEEEECCC
PKLVPEELIDLVDKLRKAGVKRVAGGLVLDKSYFAASTRDLPSFDDDVSAPYNVGPDPLL
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
YAFKAVSFTVTPGDDGKIAVDVLPPLANLSIDNQLYEGSGSCGAAAAAARPTLTAGSGIM
HEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCEECCCCCCCCHHHHCCCCEECCCCEE
TASFAGDYPLRCGARTTNLAILDHTTFFARGFLALWQQDGGTIAGPVSEGKVPTTARPLA
EEECCCCCCEECCCCCCEEEEEECHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEE
VHHSPVLGSIVYDINKFSNNVMARNLFLTIGAVAGKPPATPEQSSRAIQAFLRKSGIAMP
EECCCHHHHHHHHHHHHCCCHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCC
DLALENGSGLSRDEHVSALSLAALLQAANASPVAQAFVDSLPVAGIDGTMKNRLTNAGVL
CEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCEE
GNAHIKTGTLRDVRAIAGYVAGADGQSYVVVSFINDDHAEAARAAHDTLLEWIYAGAH
CCCEEECCCHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]