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The map label for this gene is yfcG [H]

Identifier: 115352317

GI number: 115352317

Start: 2503238

End: 2503930

Strand: Direct

Name: yfcG [H]

Synonym: Bamb_2266

Alternate gene names: 115352317

Gene position: 2503238-2503930 (Clockwise)

Preceding gene: 115352316

Following gene: 115352345

Centisome position: 70.38

GC content: 63.64

Gene sequence:

>693_bases
ATGATCGACGTCTACAGCTGGGCGACCCCGAACGGCCACAAGGTCCACATCATGCTCGAGGAAACAGGCCTCGCGTATCG
CGTGCATCCGGTCGATATCGGTGCGGGCGACCAGTTCAAGCCCGAATTCCTGAAGATCAGCCCGAACAACAAGATCCCCG
CGATCGTCGACGCGGACGGCCCAGGCGGCAAGCCGATCTCGCTGTTCGAATCGGGCGCGATCCTCGTGTACCTCGCGGAA
AAGACCGGCAAGTTCCTGCCGACCGACCCGGCCGCGCGCTATGCGACGCTCGAATGGCTGATGTTCCAGATGGGCGGCGT
CGGCCCGATGCTCGGGCAGGCGCATCACTTCCGCCTGTACGCGCCGGAGCAGATCGAATACGCGGTCAATCGCTACACGA
ACGAAGCGAAGCGCCTGTACAACGTGATGGAAAAACGCCTCGGCGAATCCGAATATCTCGCGGGCGACACGTACACGATC
GCGGACATCGCGACGTTCCCGTGGACGCGTTCGTGGCAGAACCAGGGCATCGTGCTCGATGAGCTGCCGAACGTGAAGCG
CTGGCAGGAAGCGATTGCCGCGCGTCCGGCCGTGCAGCGCGGCGTCGAAGTCCTCGCGTCGATGCGCAAGGCACTGCAGG
ACGACAAGGCGCGCGAGATGCTGTTCGGCGCGACGCAATACGCGAAGCACTGA

Upstream 100 bases:

>100_bases
CTTCCCGCCGCCTGCAGCGCACGTCGATCCATGCTCTAATGACCGCCTGTCGCGGCGTCCTGCCGGCGCCGTTTTCGTGC
ATGCAACGAAGGAGTTGACG

Downstream 100 bases:

>100_bases
AGCAGCACGCGTGGGCAACGCGGGCGGCATCCGCCGATGCCGCCGCGCGCATCAGAAGTAGTGCAGCGTAATGAATTCCG
CCGCGCAAACCAGCAACGGC

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MIDVYSWATPNGHKVHIMLEETGLAYRVHPVDIGAGDQFKPEFLKISPNNKIPAIVDADGPGGKPISLFESGAILVYLAE
KTGKFLPTDPAARYATLEWLMFQMGGVGPMLGQAHHFRLYAPEQIEYAVNRYTNEAKRLYNVMEKRLGESEYLAGDTYTI
ADIATFPWTRSWQNQGIVLDELPNVKRWQEAIAARPAVQRGVEVLASMRKALQDDKAREMLFGATQYAKH

Sequences:

>Translated_230_residues
MIDVYSWATPNGHKVHIMLEETGLAYRVHPVDIGAGDQFKPEFLKISPNNKIPAIVDADGPGGKPISLFESGAILVYLAE
KTGKFLPTDPAARYATLEWLMFQMGGVGPMLGQAHHFRLYAPEQIEYAVNRYTNEAKRLYNVMEKRLGESEYLAGDTYTI
ADIATFPWTRSWQNQGIVLDELPNVKRWQEAIAARPAVQRGVEVLASMRKALQDDKAREMLFGATQYAKH
>Mature_230_residues
MIDVYSWATPNGHKVHIMLEETGLAYRVHPVDIGAGDQFKPEFLKISPNNKIPAIVDADGPGGKPISLFESGAILVYLAE
KTGKFLPTDPAARYATLEWLMFQMGGVGPMLGQAHHFRLYAPEQIEYAVNRYTNEAKRLYNVMEKRLGESEYLAGDTYTI
ADIATFPWTRSWQNQGIVLDELPNVKRWQEAIAARPAVQRGVEVLASMRKALQDDKAREMLFGATQYAKH

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=218, Percent_Identity=54.5871559633027, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI87082195, Length=214, Percent_Identity=47.196261682243, Blast_Score=175, Evalue=2e-45,
Organism=Escherichia coli, GI87081789, Length=187, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324100, Length=234, Percent_Identity=34.6153846153846, Blast_Score=128, Evalue=7e-31,
Organism=Drosophila melanogaster, GI17933730, Length=222, Percent_Identity=27.4774774774775, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17864592, Length=223, Percent_Identity=31.390134529148, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571670, Length=173, Percent_Identity=28.9017341040462, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI17864598, Length=179, Percent_Identity=30.7262569832402, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17864594, Length=191, Percent_Identity=30.3664921465969, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI85725204, Length=194, Percent_Identity=29.3814432989691, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17737923, Length=194, Percent_Identity=29.3814432989691, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45549270, Length=196, Percent_Identity=29.0816326530612, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922160, Length=214, Percent_Identity=25.2336448598131, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24654965, Length=212, Percent_Identity=26.8867924528302, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17864596, Length=223, Percent_Identity=25.1121076233184, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24646251, Length=175, Percent_Identity=29.1428571428571, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 25826; Mature: 25826

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVYSWATPNGHKVHIMLEETGLAYRVHPVDIGAGDQFKPEFLKISPNNKIPAIVDADG
CCCEEECCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
PGGKPISLFESGAILVYLAEKTGKFLPTDPAARYATLEWLMFQMGGVGPMLGQAHHFRLY
CCCCEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCEEEEE
APEQIEYAVNRYTNEAKRLYNVMEKRLGESEYLAGDTYTIADIATFPWTRSWQNQGIVLD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEEEECCCCCCCCCCCEECC
ELPNVKRWQEAIAARPAVQRGVEVLASMRKALQDDKAREMLFGATQYAKH
CCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDVYSWATPNGHKVHIMLEETGLAYRVHPVDIGAGDQFKPEFLKISPNNKIPAIVDADG
CCCEEECCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCCEEEECCCCCCCEEEECCC
PGGKPISLFESGAILVYLAEKTGKFLPTDPAARYATLEWLMFQMGGVGPMLGQAHHFRLY
CCCCEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCEEEEE
APEQIEYAVNRYTNEAKRLYNVMEKRLGESEYLAGDTYTIADIATFPWTRSWQNQGIVLD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEEEEEECCCCCCCCCCCEECC
ELPNVKRWQEAIAARPAVQRGVEVLASMRKALQDDKAREMLFGATQYAKH
CCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]