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The map label for this gene is folD [H]

Identifier: 115352228

GI number: 115352228

Start: 2393729

End: 2394586

Strand: Direct

Name: folD [H]

Synonym: Bamb_2177

Alternate gene names: 115352228

Gene position: 2393729-2394586 (Clockwise)

Preceding gene: 115352227

Following gene: 115352229

Centisome position: 67.3

GC content: 68.07

Gene sequence:

>858_bases
ATGACAGCCCTCCTCATCGACGGCAACGCCCTCTCGAAGACCTTGCGCACGCAGGCCGCCCAACGCGCCGCCGCCCTCAC
CGCCCGCGGCCACCAGCCTGGTCTCGCGGTGGTCCTCGTCGGCGCCAACCCGGCGAGCGAAGTCTATGTGCGCAACAAGA
TCAAGGCGTGCGAGGACAACGGCTTCTTCTCGCACAAGGATGCGTACCCGGACACGCTGTCGGAAGCCGACCTGCTCGCG
CGCATCGACGAACTGAACCGCGATCCGAAGATCCACGGCATCCTGGTCCAGCTGCCGCTGCCCGCGCACATCGACAGCCA
CAAGGTGCTCGAGGCAATCGCTCCGGAGAAGGACGTCGACGGCTTCCACGTCGCGAACGCCGGCGCGCTGATGACGGGCA
AGCCGCTGTTCCGCCCGTGCACGCCGTACGGCGTGATGAAGATGTTCGAAGCGCATGACATCCCGCTGCAAGGCGCGAAC
GCGGTCGTGATCGGCCGCTCGAACATCGTCGGCAAGCCGATGGCGATGATGCTGCTCGAAGCCGGCGCGACCGTGACGAT
CTGCCACAGCAAGACGCGCGACCTCGCCGCGCACACGCGCCAGGCCGACATCGTGGTCGCCGCGGTCGGCAAGCGCAACA
TCCTGACGGCCGACATGGTCAAGCCCGGCGCGACGGTGATCGACGTCGGCATGAACCGCGACGACGCGGGCAAGCTGTGC
GGCGATGTCGACTTCGCCGGCGTGAAGGAAGTCGCCGGCCACATCACGCCGGTGCCCGGCGGCGTCGGCCCGATGACGAT
CACGATGCTGCTGATCAACACGATCGAAGCCGCCGAGCGCGCCGCCGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
GCTGTCGGACGACAGCAGGCGACCGGTATAATTGCGTGCTTTGCTGCGGCGTTTCGTGCGCCGCACACCCGCATTCCAAC
TTCCCGGCAGGACCACCACC

Downstream 100 bases:

>100_bases
TCGCCCTTGCCTGCACAGCCGGCGCGCATTCGCGCGCCGGCATCTCGCCGGTCGCCATTGCGGCCGCGCGTCACACTGTC
ACATCCCGCCGCGTTTCGCC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTALLIDGNALSKTLRTQAAQRAAALTARGHQPGLAVVLVGANPASEVYVRNKIKACEDNGFFSHKDAYPDTLSEADLLA
RIDELNRDPKIHGILVQLPLPAHIDSHKVLEAIAPEKDVDGFHVANAGALMTGKPLFRPCTPYGVMKMFEAHDIPLQGAN
AVVIGRSNIVGKPMAMMLLEAGATVTICHSKTRDLAAHTRQADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
GDVDFAGVKEVAGHITPVPGGVGPMTITMLLINTIEAAERAAAAA

Sequences:

>Translated_285_residues
MTALLIDGNALSKTLRTQAAQRAAALTARGHQPGLAVVLVGANPASEVYVRNKIKACEDNGFFSHKDAYPDTLSEADLLA
RIDELNRDPKIHGILVQLPLPAHIDSHKVLEAIAPEKDVDGFHVANAGALMTGKPLFRPCTPYGVMKMFEAHDIPLQGAN
AVVIGRSNIVGKPMAMMLLEAGATVTICHSKTRDLAAHTRQADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
GDVDFAGVKEVAGHITPVPGGVGPMTITMLLINTIEAAERAAAAA
>Mature_284_residues
TALLIDGNALSKTLRTQAAQRAAALTARGHQPGLAVVLVGANPASEVYVRNKIKACEDNGFFSHKDAYPDTLSEADLLAR
IDELNRDPKIHGILVQLPLPAHIDSHKVLEAIAPEKDVDGFHVANAGALMTGKPLFRPCTPYGVMKMFEAHDIPLQGANA
VVIGRSNIVGKPMAMMLLEAGATVTICHSKTRDLAAHTRQADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLCG
DVDFAGVKEVAGHITPVPGGVGPMTITMLLINTIEAAERAAAAA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=46.9387755102041, Blast_Score=234, Evalue=8e-62,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=45.2380952380952, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=41.3793103448276, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI36796743, Length=224, Percent_Identity=27.6785714285714, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=53.405017921147, Blast_Score=286, Evalue=8e-79,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=42.3728813559322, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=46.8531468531469, Blast_Score=259, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=41.3333333333333, Blast_Score=226, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=294, Percent_Identity=25.8503401360544, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=48.6394557823129, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=48.6394557823129, Blast_Score=255, Evalue=2e-68,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=45.3287197231834, Blast_Score=252, Evalue=2e-67,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=45.3287197231834, Blast_Score=252, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=45.3287197231834, Blast_Score=252, Evalue=2e-67,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=45.3287197231834, Blast_Score=252, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 29925; Mature: 29794

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALLIDGNALSKTLRTQAAQRAAALTARGHQPGLAVVLVGANPASEVYVRNKIKACEDN
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHEEHHCCCCCCCC
GFFSHKDAYPDTLSEADLLARIDELNRDPKIHGILVQLPLPAHIDSHKVLEAIAPEKDVD
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHVANAGALMTGKPLFRPCTPYGVMKMFEAHDIPLQGANAVVIGRSNIVGKPMAMMLLE
CEEEECCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHH
AGATVTICHSKTRDLAAHTRQADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
CCCEEEEECCCCHHHHHHCCCCCEEEEECCCCCEEEHHCCCCCCEEEEECCCCCCHHHHC
GDVDFAGVKEVAGHITPVPGGVGPMTITMLLINTIEAAERAAAAA
CCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TALLIDGNALSKTLRTQAAQRAAALTARGHQPGLAVVLVGANPASEVYVRNKIKACEDN
EEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHEEHHCCCCCCCC
GFFSHKDAYPDTLSEADLLARIDELNRDPKIHGILVQLPLPAHIDSHKVLEAIAPEKDVD
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHVANAGALMTGKPLFRPCTPYGVMKMFEAHDIPLQGANAVVIGRSNIVGKPMAMMLLE
CEEEECCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHH
AGATVTICHSKTRDLAAHTRQADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
CCCEEEEECCCCHHHHHHCCCCCEEEEECCCCCEEEHHCCCCCCEEEEECCCCCCHHHHC
GDVDFAGVKEVAGHITPVPGGVGPMTITMLLINTIEAAERAAAAA
CCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA