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The map label for this gene is mls [H]

Identifier: 115352135

GI number: 115352135

Start: 2286747

End: 2288339

Strand: Direct

Name: mls [H]

Synonym: Bamb_2084

Alternate gene names: 115352135

Gene position: 2286747-2288339 (Clockwise)

Preceding gene: 115352134

Following gene: 115352137

Centisome position: 64.3

GC content: 64.97

Gene sequence:

>1593_bases
ATGAGCACCCCGATCACGCTGCCGCAAGGCATGGCGATCACCGGCGAAATCAAGCCGGGTTACGAAGCAATCCTGACGCC
TGACGCGCTCGCACTCGTCGCGGCGCTGCACCGCACGTTCGAGCCGCGTCGCCAGGCGCTGCTGCAGGCCCGCGTCGAAC
GCACCAAGCGCCTCGACGCCGGCGAACGCCCCGATTTCCTGGCCGACACGAAGGCGATCCGCGAAGGCGACTGGAAGGTC
GCGCCGCTGCCGGCCGACCTGCAATGCCGCCGTGTCGAGATCACGGGCCCCGTCGAGCGCAAGATGATCATCAACGCGCT
GAACTCCGGCGCCGATTCGTACATGACGGACTTCGAGGACTCGAACGCGCCGAGCTGGACGAACCAGATCGACGGCCAGA
TCAACCTGAAGGACGCGGTGCGCCGCACGATCTCGCTCGAGCAGAACGGCAAGTCGTACCGGCTGAACGACAAGGTCGCG
ACGCTGATCGTGCGTCCGCGCGGCTGGCATCTCGACGAGAAGCACGTGACGGTCGACGGCCAGCGCGTGTCCGGCGGCAT
CTTCGATTTCGCGCTGTTCCTGTTCCACAACGCGAAGGAATTGATCGCGCGCGGCTCGGGCCCGTACTTCTACCTGCCGA
AGATGGAAAGCCATCTCGAAGCGCGCCTGTGGAACGACATCTTCGTCGCGGCACAGGAAGCGGTCGGCGTGCCGCGCGGC
ACGATCCGCGCGACGGTGCTGATCGAGACGATCCTCGCCGCGTTCGAGATGGACGAGATCCTGTACGAACTGCGCGAACA
CAGCTCGGGCCTGAATGCCGGCCGCTGGGACTACATCTTCTCGGCGATCAAAAAGTTCAAGAACGACCGCGACTTCTGCC
TGGCCGACCGCGCGAAGATCACGATGACGGTGCCGTTCATGCGCGCGTACGCGTTGCTGCTGCTGAAGACCTGCCACAAG
CGCAACGCACCGGCAATCGGCGGGATGAGCGCGCTGATTCCGATCAAGAACGATCCGCAGGCGAACGAGAAGGCGATGGG
CGGCGTGCGCTCGGACAAGCAGCGCGACGCGACCGACGGCTACGACGGCGGCTGGGTCGCGCACCCGGGCCTCGTGCCGA
TCGCGATGGAGGAATTCGTCAAGGTGCTCGGCGACAAGCCGAACCAGATCGCCAAGCAGCGCGACGACGTGCAGATCGAA
GGCAAGAATCTGCTCGACTTCCAGCCTGAAGCGCCGATCACCGAAGCCGGCCTGCGCAACAACATCAACGTCGGCATTCA
CTATCTCGGCGCGTGGCTCGACGGCAACGGCTGCGTGCCGATCCACAACCTGATGGAAGACGCGGCCACCGCCGAAATCT
CCCGCTCGCAGGTGTGGCAGTGGATCCGCTCGCCGAAGGGCGTGCTCGACGACGGCCGCAAGGTCACGGCGGAACTCGTG
CGCGAATTCGCGAAGGCCGAGCTCGACAACGTGAAGCGTTCCGTCGGCGGCAACACGCAGCCCTACGAGCGTGCGGCGGC
GATCTTCGAACAGATGTCGACGTCGGAAGGCTTCACCGAATTCCTGACGCTGCCGCTGTACGAGGAAATCTGA

Upstream 100 bases:

>100_bases
GCGAACCGCGCGCGCCCCGGCCCGGGCGCATGAGGGTCGCGGCGCTGCCGCCTTCCCCCTTCACCGAAACCGCAACTGAC
CGACCAAGGAGATGAGACTC

Downstream 100 bases:

>100_bases
CGATGCGGGCCGGGTCCTGCCGGCCCGGTCGTGCCCGACCTGCCCGCCGCCGGTCGCACCGGCCGGCGGCATGAAAAGCG
CCCGCCATGAAAAACACCCC

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSTPITLPQGMAITGEIKPGYEAILTPDALALVAALHRTFEPRRQALLQARVERTKRLDAGERPDFLADTKAIREGDWKV
APLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFEDSNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYRLNDKVA
TLIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLFHNAKELIARGSGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRG
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRAKITMTVPFMRAYALLLLKTCHK
RNAPAIGGMSALIPIKNDPQANEKAMGGVRSDKQRDATDGYDGGWVAHPGLVPIAMEEFVKVLGDKPNQIAKQRDDVQIE
GKNLLDFQPEAPITEAGLRNNINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELV
REFAKAELDNVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI

Sequences:

>Translated_530_residues
MSTPITLPQGMAITGEIKPGYEAILTPDALALVAALHRTFEPRRQALLQARVERTKRLDAGERPDFLADTKAIREGDWKV
APLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFEDSNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYRLNDKVA
TLIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLFHNAKELIARGSGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRG
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRAKITMTVPFMRAYALLLLKTCHK
RNAPAIGGMSALIPIKNDPQANEKAMGGVRSDKQRDATDGYDGGWVAHPGLVPIAMEEFVKVLGDKPNQIAKQRDDVQIE
GKNLLDFQPEAPITEAGLRNNINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELV
REFAKAELDNVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI
>Mature_529_residues
STPITLPQGMAITGEIKPGYEAILTPDALALVAALHRTFEPRRQALLQARVERTKRLDAGERPDFLADTKAIREGDWKVA
PLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFEDSNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYRLNDKVAT
LIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLFHNAKELIARGSGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRGT
IRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRAKITMTVPFMRAYALLLLKTCHKR
NAPAIGGMSALIPIKNDPQANEKAMGGVRSDKQRDATDGYDGGWVAHPGLVPIAMEEFVKVLGDKPNQIAKQRDDVQIEG
KNLLDFQPEAPITEAGLRNNINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELVR
EFAKAELDNVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=536, Percent_Identity=48.8805970149254, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17561814, Length=524, Percent_Identity=51.3358778625954, Blast_Score=527, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI71982926, Length=420, Percent_Identity=53.3333333333333, Blast_Score=443, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6324212, Length=514, Percent_Identity=52.7237354085603, Blast_Score=539, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6322222, Length=514, Percent_Identity=50.9727626459144, Blast_Score=513, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 59112; Mature: 58981

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPITLPQGMAITGEIKPGYEAILTPDALALVAALHRTFEPRRQALLQARVERTKRLDA
CCCCCCCCCCCEEEECCCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
GERPDFLADTKAIREGDWKVAPLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFED
CCCCCHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHCCCC
SNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYRLNDKVATLIVRPRGWHLDEKHVTVDG
CCCCCCHHCCCCEEEHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCCCCCEEEECC
QRVSGGIFDFALFLFHNAKELIARGSGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRG
CCCCCHHHHHHHHHHHCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRAKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEE
TMTVPFMRAYALLLLKTCHKRNAPAIGGMSALIPIKNDPQANEKAMGGVRSDKQRDATDG
EEECHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCC
YDGGWVAHPGLVPIAMEEFVKVLGDKPNQIAKQRDDVQIEGKNLLDFQPEAPITEAGLRN
CCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEECCCCEECCCCCCCCHHHCCCC
NINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELV
CCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
REFAKAELDNVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHCC
>Mature Secondary Structure 
STPITLPQGMAITGEIKPGYEAILTPDALALVAALHRTFEPRRQALLQARVERTKRLDA
CCCCCCCCCCEEEECCCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
GERPDFLADTKAIREGDWKVAPLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFED
CCCCCHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHCCCC
SNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYRLNDKVATLIVRPRGWHLDEKHVTVDG
CCCCCCHHCCCCEEEHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCCCCCEEEECC
QRVSGGIFDFALFLFHNAKELIARGSGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRG
CCCCCHHHHHHHHHHHCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRAKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEE
TMTVPFMRAYALLLLKTCHKRNAPAIGGMSALIPIKNDPQANEKAMGGVRSDKQRDATDG
EEECHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCC
YDGGWVAHPGLVPIAMEEFVKVLGDKPNQIAKQRDDVQIEGKNLLDFQPEAPITEAGLRN
CCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEECCCCEECCCCCCCCHHHCCCC
NINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELV
CCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
REFAKAELDNVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA