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The map label for this gene is ynfL [H]

Identifier: 115352051

GI number: 115352051

Start: 2196724

End: 2197665

Strand: Direct

Name: ynfL [H]

Synonym: Bamb_2000

Alternate gene names: 115352051

Gene position: 2196724-2197665 (Clockwise)

Preceding gene: 115352050

Following gene: 115352052

Centisome position: 61.77

GC content: 63.38

Gene sequence:

>942_bases
ATGGAACTTCGGCACCTGCGTTGCTTCCTTGCCGTCGCCGAAGAGCTGCATTTCGCCCGTGCGGCGGAGAAATTGCATGT
CGAGCAATCGCCTTTATCGCGGGCCATCAAGGAACTGGAAGAAGAACTGGGCGTGGTGCTGTTTGCGCGAACTACGCGCA
GCACTCGGTTGACCCGTGCGGGCAAGCTGTTTTTAGAGCATGTGCCGCGCGTCTTCTCTGCCTTGCAGCAGGCCCGCGAC
AGCGTGAAGGCAGCGGCCAACGGCTTCCACAGCCAATTGCGCGTTGCGCTGTCCGACGGCATGACGCCCTCACGTCTGCC
GGCTTTGCTGGCGCTCTGTCGGCAGGAGGAGCCCGAAGTCGAAATCCGTTTCTTTGAGGTGCCGCTGGCGCAACAGATCA
AAGGGCTGTACGGCGATCTGTACGACGTAGGCTTCGCCCGCGCCGATGAAGTCGGCGATGGCCTCATTGCCGAGCCGGTA
TGGAGTGATCCTCTGATGGTGGCCGTGCCGGCACGGCATCCCCTGCTGGCCCACAAGCGCATCCCTTTGGAAGAATTGCT
GCGCTATCCCCTGGTGCTGTGCGATCCGCAAGCGTGCGAGGGCCATGCCCGGCAGGTGGATCGTGTGCTGCGCCGTGTGG
ACATGGAACCGCTGGTGGCTGAACGGGTGGCGTCCTGCGACCTGATGATGGCGCTGGTGTCCGCAGGCATCGCTCTGGGG
CTGACGGGCTGCGCGCACATCTTGGCCAGCCAGGAACCTGGCGTAGTGGCACGGCCACTGACAGGCCGCTCACCGTTGCT
GACGACCTACTTGCTGCGTCCGGAAGGCGAGCCTTCGGAAATGCTCGCCCGCTTCATCGACCGTGTGCAGGCCATCGAAT
CACCCGAAGGTAGCAGGCCCACGCCGGGTCTTGATGCCGACTCTCTGGAGGAACCCGCATGA

Upstream 100 bases:

>100_bases
TCATCTTCGAGGGTTTGCCTACGGCAATGATCAGCGCCCCGGAATCTGTAACGGGGCCATTCCTTTGGCGCCATTTGAAT
GGGCAACTGTGAGGTGACGC

Downstream 100 bases:

>100_bases
TGAGAAACTCCATTCCGTTCCTGCTGGCCGTGGCATTAACCGCTTGCGGCCAATCGGAAACGCCCAAGCAGGCCGAGGTG
TCGACCGTGGAGGAACTGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MELRHLRCFLAVAEELHFARAAEKLHVEQSPLSRAIKELEEELGVVLFARTTRSTRLTRAGKLFLEHVPRVFSALQQARD
SVKAAANGFHSQLRVALSDGMTPSRLPALLALCRQEEPEVEIRFFEVPLAQQIKGLYGDLYDVGFARADEVGDGLIAEPV
WSDPLMVAVPARHPLLAHKRIPLEELLRYPLVLCDPQACEGHARQVDRVLRRVDMEPLVAERVASCDLMMALVSAGIALG
LTGCAHILASQEPGVVARPLTGRSPLLTTYLLRPEGEPSEMLARFIDRVQAIESPEGSRPTPGLDADSLEEPA

Sequences:

>Translated_313_residues
MELRHLRCFLAVAEELHFARAAEKLHVEQSPLSRAIKELEEELGVVLFARTTRSTRLTRAGKLFLEHVPRVFSALQQARD
SVKAAANGFHSQLRVALSDGMTPSRLPALLALCRQEEPEVEIRFFEVPLAQQIKGLYGDLYDVGFARADEVGDGLIAEPV
WSDPLMVAVPARHPLLAHKRIPLEELLRYPLVLCDPQACEGHARQVDRVLRRVDMEPLVAERVASCDLMMALVSAGIALG
LTGCAHILASQEPGVVARPLTGRSPLLTTYLLRPEGEPSEMLARFIDRVQAIESPEGSRPTPGLDADSLEEPA
>Mature_313_residues
MELRHLRCFLAVAEELHFARAAEKLHVEQSPLSRAIKELEEELGVVLFARTTRSTRLTRAGKLFLEHVPRVFSALQQARD
SVKAAANGFHSQLRVALSDGMTPSRLPALLALCRQEEPEVEIRFFEVPLAQQIKGLYGDLYDVGFARADEVGDGLIAEPV
WSDPLMVAVPARHPLLAHKRIPLEELLRYPLVLCDPQACEGHARQVDRVLRRVDMEPLVAERVASCDLMMALVSAGIALG
LTGCAHILASQEPGVVARPLTGRSPLLTTYLLRPEGEPSEMLARFIDRVQAIESPEGSRPTPGLDADSLEEPA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=242, Percent_Identity=34.297520661157, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=33.2075471698113, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=32.5423728813559, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI157672245, Length=230, Percent_Identity=31.304347826087, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790399, Length=199, Percent_Identity=27.1356783919598, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87081904, Length=196, Percent_Identity=30.1020408163265, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790208, Length=244, Percent_Identity=27.4590163934426, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786713, Length=203, Percent_Identity=33.4975369458128, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI145693105, Length=196, Percent_Identity=27.0408163265306, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788748, Length=240, Percent_Identity=25.8333333333333, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787601, Length=236, Percent_Identity=23.3050847457627, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787806, Length=303, Percent_Identity=24.7524752475248, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI48994962, Length=131, Percent_Identity=34.3511450381679, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787949, Length=148, Percent_Identity=31.7567567567568, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787530, Length=264, Percent_Identity=24.2424242424242, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082132, Length=185, Percent_Identity=30.2702702702703, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788296, Length=251, Percent_Identity=27.4900398406374, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=243, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1786401, Length=160, Percent_Identity=31.25, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34491; Mature: 34491

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRCFLAVAEELHFARAAEKLHVEQSPLSRAIKELEEELGVVLFARTTRSTRLTRA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHH
GKLFLEHVPRVFSALQQARDSVKAAANGFHSQLRVALSDGMTPSRLPALLALCRQEEPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCE
EIRFFEVPLAQQIKGLYGDLYDVGFARADEVGDGLIAEPVWSDPLMVAVPARHPLLAHKR
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHCCC
IPLEELLRYPLVLCDPQACEGHARQVDRVLRRVDMEPLVAERVASCDLMMALVSAGIALG
CCHHHHHHCCEEEECCHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTGCAHILASQEPGVVARPLTGRSPLLTTYLLRPEGEPSEMLARFIDRVQAIESPEGSRP
HHHHHHHHHCCCCCCEECCCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
TPGLDADSLEEPA
CCCCCCCCCCCCC
>Mature Secondary Structure
MELRHLRCFLAVAEELHFARAAEKLHVEQSPLSRAIKELEEELGVVLFARTTRSTRLTRA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHH
GKLFLEHVPRVFSALQQARDSVKAAANGFHSQLRVALSDGMTPSRLPALLALCRQEEPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCE
EIRFFEVPLAQQIKGLYGDLYDVGFARADEVGDGLIAEPVWSDPLMVAVPARHPLLAHKR
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHCCC
IPLEELLRYPLVLCDPQACEGHARQVDRVLRRVDMEPLVAERVASCDLMMALVSAGIALG
CCHHHHHHCCEEEECCHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTGCAHILASQEPGVVARPLTGRSPLLTTYLLRPEGEPSEMLARFIDRVQAIESPEGSRP
HHHHHHHHHCCCCCCEECCCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
TPGLDADSLEEPA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]