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The map label for this gene is oprM [H]

Identifier: 115351714

GI number: 115351714

Start: 1837976

End: 1839586

Strand: Direct

Name: oprM [H]

Synonym: Bamb_1662

Alternate gene names: 115351714

Gene position: 1837976-1839586 (Clockwise)

Preceding gene: 115351713

Following gene: 115351715

Centisome position: 51.68

GC content: 72.75

Gene sequence:

>1611_bases
ATGGAACTGTCGATGAACCTGAAAACCAAGGCCGCGCGAGCGCTCGCGGCCGCGAGCCTCGCCGGGCCGCTGGCCGGCTG
CGCATGGTTCGCGCCGAGCGGCGAGCCGCCCGCGATGCCGTCGCCCGCGCATTACGGCGCGACGCCGCAATCGCAGCAGA
CCGTCGCCGCGCAAGGCGTCGCGCAGCAGTTCGAGGTCGGCGCGCAGCCGGTACCAGACTGGTGGACGCAGTACCGTTCC
GATGCGCTGAACGCGCTCGTCGACGAAGGGCTGCGCAACAGCCCGACGCTCGGCGCGGCGTCGCATTCGCTGGATGCCGC
GCGCGAGCAGCTGCGCGGGCAGATCGGCAGCTCGATGCTGCCGTCGATCGACGCGGGCGCCCAGGCCACGCGCCAGCGCG
CGCTCGGCGTGCCGATTCCCGCCCTCGGCGCGCCGACGCTGCTGTACGACACGTTCGTCGGGCAATTGCAGGCGAGCTAC
ACGATCGACCTGTTCGGCGTGTCGCGCTTCGCGAATCGCGCGCTCGCGAAACGCGTCGACGTCAGTGCGTTCCAGCTCGA
ATCCGCCCGGCGCGCGCTGGCCGCGAACATCGTCACCACGTCGATCACCGTGGCGGTGCTCAATGCGCAGATCGAGACGA
CCGAGCGGCTCGTCGCGCTCGCGAACGATCAGGCGCACGACGCCGAACGCCGCCATGCGCTCGGTTCGGCGTCGCGCAGC
GACGCGCTGAGTGCGCGGCAAAGCGCCGACACGTTCGCGGCGAGCCTGCCCGCATTGCGCCAGCAACGCGACTCGGCCCG
CCATGCGCTCGCGGTGCTGGTCGGCCGCACGCCCGACCGGCCGCCGGCCGATCTCGCGCTCGCCGATCTGCACTTGCCGG
AGCAGGTGCCCGTCGTCGTGCCGTCGGATCTGCTGCAAAGCCGCCCGGACATCCAGGCCGCCGACGCCGGGCTGAAGGCG
GCCGCGGCCGAAGTGGGCCTCGCGACCGCGCAGATGTTTCCGCAGTTGTCGCTGTCGGCGGCGATGGGCAAAGGCGGCTT
CAGCTGGCCGACGATGCTGTCGGGCGCCGGTTCGATCTGGAACGTCGGCGCGTCGCTGAGCCAGCCGATCTTCCACGGCG
GTGCGCTGCTCGCCCAGCGTCGGGCGGCGAAGGCGACCTACGAGGCCGCGGTCGATCAGTACAAGCAGGCCGTGCTCGGC
GCATTCCAGAACGTCGCCGATTCGCTCGCGGCGCTCGAGCACGATGCTGAGGCGCTCGACGCGTCGTCGCACGCCGCGCT
TTCCGCGCGCGGCGCGTACGACGACGCGGCCGCCCGCGTGCGGCTCGGCGCGCTGCCGCCGTCGGCCGCGCGCGCGAGCG
AACTGCAGTACCGCAATGCGCGGCTCGACGAGCTTCGCGCGACCGGCGCACGGTTCGCGGATACCGCACGGCTTTACCAG
GCGATGGGCACGCCGCCGGTCGACGGCGCGGGCGTCGGCGTCGGCAAGGCCGTGAAAACCGACCAAGCCGGCAATCCCGG
CAACGCGGCTCACGACGCCCCCGCCACCCAGGCGCGCACGGCCGCGCCCGATGCGCCGCCGTCACCACCTTCGCCGCCGT
CCCCCCAATAA

Upstream 100 bases:

>100_bases
CCGCGCTGTACGCCGCGTGGTTCCGCGTGAAGCGCGACGAACGCGACCCGCACGAACCGCCGCACGACGGCACACCTGCC
GCGCCGTCGGGAGCCTGACC

Downstream 100 bases:

>100_bases
CGCGCGGTTTCCTTCAGGCGCACGGCGCGCGGGGCCATCCCGCGCGCCGATCCGCGATCTTCCGCTTGACCCTCACGTAG
CGTAACGTTCGAGACTCCAG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MELSMNLKTKAARALAAASLAGPLAGCAWFAPSGEPPAMPSPAHYGATPQSQQTVAAQGVAQQFEVGAQPVPDWWTQYRS
DALNALVDEGLRNSPTLGAASHSLDAAREQLRGQIGSSMLPSIDAGAQATRQRALGVPIPALGAPTLLYDTFVGQLQASY
TIDLFGVSRFANRALAKRVDVSAFQLESARRALAANIVTTSITVAVLNAQIETTERLVALANDQAHDAERRHALGSASRS
DALSARQSADTFAASLPALRQQRDSARHALAVLVGRTPDRPPADLALADLHLPEQVPVVVPSDLLQSRPDIQAADAGLKA
AAAEVGLATAQMFPQLSLSAAMGKGGFSWPTMLSGAGSIWNVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLG
AFQNVADSLAALEHDAEALDASSHAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDELRATGARFADTARLYQ
AMGTPPVDGAGVGVGKAVKTDQAGNPGNAAHDAPATQARTAAPDAPPSPPSPPSPQ

Sequences:

>Translated_536_residues
MELSMNLKTKAARALAAASLAGPLAGCAWFAPSGEPPAMPSPAHYGATPQSQQTVAAQGVAQQFEVGAQPVPDWWTQYRS
DALNALVDEGLRNSPTLGAASHSLDAAREQLRGQIGSSMLPSIDAGAQATRQRALGVPIPALGAPTLLYDTFVGQLQASY
TIDLFGVSRFANRALAKRVDVSAFQLESARRALAANIVTTSITVAVLNAQIETTERLVALANDQAHDAERRHALGSASRS
DALSARQSADTFAASLPALRQQRDSARHALAVLVGRTPDRPPADLALADLHLPEQVPVVVPSDLLQSRPDIQAADAGLKA
AAAEVGLATAQMFPQLSLSAAMGKGGFSWPTMLSGAGSIWNVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLG
AFQNVADSLAALEHDAEALDASSHAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDELRATGARFADTARLYQ
AMGTPPVDGAGVGVGKAVKTDQAGNPGNAAHDAPATQARTAAPDAPPSPPSPPSPQ
>Mature_536_residues
MELSMNLKTKAARALAAASLAGPLAGCAWFAPSGEPPAMPSPAHYGATPQSQQTVAAQGVAQQFEVGAQPVPDWWTQYRS
DALNALVDEGLRNSPTLGAASHSLDAAREQLRGQIGSSMLPSIDAGAQATRQRALGVPIPALGAPTLLYDTFVGQLQASY
TIDLFGVSRFANRALAKRVDVSAFQLESARRALAANIVTTSITVAVLNAQIETTERLVALANDQAHDAERRHALGSASRS
DALSARQSADTFAASLPALRQQRDSARHALAVLVGRTPDRPPADLALADLHLPEQVPVVVPSDLLQSRPDIQAADAGLKA
AAAEVGLATAQMFPQLSLSAAMGKGGFSWPTMLSGAGSIWNVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLG
AFQNVADSLAALEHDAEALDASSHAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDELRATGARFADTARLYQ
AMGTPPVDGAGVGVGKAVKTDQAGNPGNAAHDAPATQARTAAPDAPPSPPSPPSPQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=466, Percent_Identity=25.9656652360515, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI2367348, Length=395, Percent_Identity=24.8101265822785, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55388; Mature: 55388

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSMNLKTKAARALAAASLAGPLAGCAWFAPSGEPPAMPSPAHYGATPQSQQTVAAQGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AQQFEVGAQPVPDWWTQYRSDALNALVDEGLRNSPTLGAASHSLDAAREQLRGQIGSSML
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHC
PSIDAGAQATRQRALGVPIPALGAPTLLYDTFVGQLQASYTIDLFGVSRFANRALAKRVD
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHC
VSAFQLESARRALAANIVTTSITVAVLNAQIETTERLVALANDQAHDAERRHALGSASRS
HHHHHHHHHHHHHHHHHHHHHEEEHEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHH
DALSARQSADTFAASLPALRQQRDSARHALAVLVGRTPDRPPADLALADLHLPEQVPVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHCCCCCCCCEEC
PSDLLQSRPDIQAADAGLKAAAAEVGLATAQMFPQLSLSAAMGKGGFSWPTMLSGAGSIW
CHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHH
NVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLGAFQNVADSLAALEHDAEALD
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASSHAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDELRATGARFADTARLYQ
CCCCHHHHCCCCCCHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AMGTPPVDGAGVGVGKAVKTDQAGNPGNAAHDAPATQARTAAPDAPPSPPSPPSPQ
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MELSMNLKTKAARALAAASLAGPLAGCAWFAPSGEPPAMPSPAHYGATPQSQQTVAAQGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AQQFEVGAQPVPDWWTQYRSDALNALVDEGLRNSPTLGAASHSLDAAREQLRGQIGSSML
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHC
PSIDAGAQATRQRALGVPIPALGAPTLLYDTFVGQLQASYTIDLFGVSRFANRALAKRVD
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHC
VSAFQLESARRALAANIVTTSITVAVLNAQIETTERLVALANDQAHDAERRHALGSASRS
HHHHHHHHHHHHHHHHHHHHHEEEHEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHH
DALSARQSADTFAASLPALRQQRDSARHALAVLVGRTPDRPPADLALADLHLPEQVPVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHCCCCCCCCEEC
PSDLLQSRPDIQAADAGLKAAAAEVGLATAQMFPQLSLSAAMGKGGFSWPTMLSGAGSIW
CHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHH
NVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLGAFQNVADSLAALEHDAEALD
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASSHAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDELRATGARFADTARLYQ
CCCCHHHHCCCCCCHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AMGTPPVDGAGVGVGKAVKTDQAGNPGNAAHDAPATQARTAAPDAPPSPPSPPSPQ
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]