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The map label for this gene is nolF [H]

Identifier: 115351712

GI number: 115351712

Start: 1833724

End: 1834833

Strand: Direct

Name: nolF [H]

Synonym: Bamb_1660

Alternate gene names: 115351712

Gene position: 1833724-1834833 (Clockwise)

Preceding gene: 115351710

Following gene: 115351713

Centisome position: 51.56

GC content: 72.34

Gene sequence:

>1110_bases
GTGAATCGCTCCGGTTCCCGCGCCGTGCTGCTGATCGGCACCGCGCTCGTCCTTGCCGCCTGTCATCCGAAGGAAGCCGC
GCCGCCCGCGCCGCGCCCGGTCGTCGCGCTGCCCGTGCACGCCGACGGCGCCGCGGTCGCGACCACGCTGCCCGGCGAGA
TCCAGCCCCGTTATGCGACGCCGCTGTCGTTCCGCATCGCCGGCAAGATCATCGAGCGCAAGGTGCGGCTCGGCGATACG
GTCAAGGCCGGCCAGGTCGTCGCGCTGCTCGATCCGTCCGACGTCGAGAAGAACGCCGCGAGTGCGCAGGCGCAATTCGA
TGCCGCGACGCACAGCCTCGCGTTCGCGAAGCAGCAGCTCGAGCGCGACCGCGCACAGGCGCGCGAGAACCTGATCGCGA
CCGCGCAGCTCGAACAGACCCAGAACAGCTACACGTCGGCGCTCGCGCAGCGCGACCAGGCGCAGCAGCAGCTCGCGCTC
GCGAAGAACCAGCTCCGCTATGCGACGCTCGTCGCCGATCACGCGGGCACCATCACCGCGGAACAGGCCGACACCGGCCA
GAACGTCTCGGCCGGCCAGGCCGTCTACCAGCTCGCGTGGTCGGGCGACGTCGACGTCGTCAGCGACGTGCCCGAGGCCG
CGCTCGCGTCGCTCACGCCCGGCCATGCGGCGAGCGTCACGCTGCCGTCGCTGCCCGGCCGCCAGTTCGCCGCGAAGGTG
CGCGAGATCGCGCCGGCCGCCGATCCGCAGAGCCGCACCTATCGCGTGAAGCTCACGCTCGCCGCGCCCGATCCGGCCGT
CCGCCTCGGGATGACCACGAACGTCGCGTTCGACGGCGCACCGGTTGCCGGCAACGCGCCGAGCATCACGCTGCCCGCGA
CCGCGCTGTTCCACGACGGCCCGCAGCCGGCCGTGTGGGTCGTGCGCACCAAGGACGACACGCTCGAGTTGCGCCGCGTC
GACGTCGCACGCTTCAACGAACGCACCGTCACCGTGTCGCACGGGCTGCAGCCTGGCGAACGCGTCGTGCTGCAGGGCGT
GCACACGGTCAGCGCGGGCGAGAAGGTGCGGCCGGTCGCGCCGCTGCACCCGCAGGACTTCGCGTCATGA

Upstream 100 bases:

>100_bases
TCGTTCGGCACTGTCCGTGCGCGCCCACCCGGCGCCACTCGGGGCAAGCGCCCATCGCGCTTGCCGGCTTCGTTCCGCTC
TCACCTGATCTGGGGGTTTC

Downstream 100 bases:

>100_bases
GCGGCCCCCGCGAAGAAGGCCGCTTCAACCTGTCGGCATGGGCGCTGCGCCACCAGGCGCTGGTCGTCTACCTGATCGCG
CTCGCGACGCTCGCGGGCAT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNRSGSRAVLLIGTALVLAACHPKEAAPPAPRPVVALPVHADGAAVATTLPGEIQPRYATPLSFRIAGKIIERKVRLGDT
VKAGQVVALLDPSDVEKNAASAQAQFDAATHSLAFAKQQLERDRAQARENLIATAQLEQTQNSYTSALAQRDQAQQQLAL
AKNQLRYATLVADHAGTITAEQADTGQNVSAGQAVYQLAWSGDVDVVSDVPEAALASLTPGHAASVTLPSLPGRQFAAKV
REIAPAADPQSRTYRVKLTLAAPDPAVRLGMTTNVAFDGAPVAGNAPSITLPATALFHDGPQPAVWVVRTKDDTLELRRV
DVARFNERTVTVSHGLQPGERVVLQGVHTVSAGEKVRPVAPLHPQDFAS

Sequences:

>Translated_369_residues
MNRSGSRAVLLIGTALVLAACHPKEAAPPAPRPVVALPVHADGAAVATTLPGEIQPRYATPLSFRIAGKIIERKVRLGDT
VKAGQVVALLDPSDVEKNAASAQAQFDAATHSLAFAKQQLERDRAQARENLIATAQLEQTQNSYTSALAQRDQAQQQLAL
AKNQLRYATLVADHAGTITAEQADTGQNVSAGQAVYQLAWSGDVDVVSDVPEAALASLTPGHAASVTLPSLPGRQFAAKV
REIAPAADPQSRTYRVKLTLAAPDPAVRLGMTTNVAFDGAPVAGNAPSITLPATALFHDGPQPAVWVVRTKDDTLELRRV
DVARFNERTVTVSHGLQPGERVVLQGVHTVSAGEKVRPVAPLHPQDFAS
>Mature_369_residues
MNRSGSRAVLLIGTALVLAACHPKEAAPPAPRPVVALPVHADGAAVATTLPGEIQPRYATPLSFRIAGKIIERKVRLGDT
VKAGQVVALLDPSDVEKNAASAQAQFDAATHSLAFAKQQLERDRAQARENLIATAQLEQTQNSYTSALAQRDQAQQQLAL
AKNQLRYATLVADHAGTITAEQADTGQNVSAGQAVYQLAWSGDVDVVSDVPEAALASLTPGHAASVTLPSLPGRQFAAKV
REIAPAADPQSRTYRVKLTLAAPDPAVRLGMTTNVAFDGAPVAGNAPSITLPATALFHDGPQPAVWVVRTKDDTLELRRV
DVARFNERTVTVSHGLQPGERVVLQGVHTVSAGEKVRPVAPLHPQDFAS

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=325, Percent_Identity=25.2307692307692, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789929, Length=384, Percent_Identity=21.6145833333333, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87081796, Length=301, Percent_Identity=25.5813953488372, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38867; Mature: 38867

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSGSRAVLLIGTALVLAACHPKEAAPPAPRPVVALPVHADGAAVATTLPGEIQPRYAT
CCCCCCEEEEEEEHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCC
PLSFRIAGKIIERKVRLGDTVKAGQVVALLDPSDVEKNAASAQAQFDAATHSLAFAKQQL
CEEHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ERDRAQARENLIATAQLEQTQNSYTSALAQRDQAQQQLALAKNQLRYATLVADHAGTITA
HHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEE
EQADTGQNVSAGQAVYQLAWSGDVDVVSDVPEAALASLTPGHAASVTLPSLPGRQFAAKV
CCCCCCCCCCCCCEEEEEECCCCCHHHHCCCHHHHHHCCCCCCCEEECCCCCCHHHHHHH
REIAPAADPQSRTYRVKLTLAAPDPAVRLGMTTNVAFDGAPVAGNAPSITLPATALFHDG
HHHCCCCCCCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCEEECCCEEECCC
PQPAVWVVRTKDDTLELRRVDVARFNERTVTVSHGLQPGERVVLQGVHTVSAGEKVRPVA
CCCEEEEEEECCCCEEEEEEHHHHCCCEEEEEECCCCCCHHHHEEECEEECCCCCCCCCC
PLHPQDFAS
CCCCHHCCC
>Mature Secondary Structure
MNRSGSRAVLLIGTALVLAACHPKEAAPPAPRPVVALPVHADGAAVATTLPGEIQPRYAT
CCCCCCEEEEEEEHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCC
PLSFRIAGKIIERKVRLGDTVKAGQVVALLDPSDVEKNAASAQAQFDAATHSLAFAKQQL
CEEHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ERDRAQARENLIATAQLEQTQNSYTSALAQRDQAQQQLALAKNQLRYATLVADHAGTITA
HHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEE
EQADTGQNVSAGQAVYQLAWSGDVDVVSDVPEAALASLTPGHAASVTLPSLPGRQFAAKV
CCCCCCCCCCCCCEEEEEECCCCCHHHHCCCHHHHHHCCCCCCCEEECCCCCCHHHHHHH
REIAPAADPQSRTYRVKLTLAAPDPAVRLGMTTNVAFDGAPVAGNAPSITLPATALFHDG
HHHCCCCCCCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCEEECCCEEECCC
PQPAVWVVRTKDDTLELRRVDVARFNERTVTVSHGLQPGERVVLQGVHTVSAGEKVRPVA
CCCEEEEEEECCCCEEEEEEHHHHCCCEEEEEECCCCCCHHHHEEECEEECCCCCCCCCC
PLHPQDFAS
CCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]