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The map label for this gene is amiD [H]

Identifier: 115351502

GI number: 115351502

Start: 1599411

End: 1600313

Strand: Direct

Name: amiD [H]

Synonym: Bamb_1449

Alternate gene names: 115351502

Gene position: 1599411-1600313 (Clockwise)

Preceding gene: 115351501

Following gene: 115351503

Centisome position: 44.97

GC content: 67.55

Gene sequence:

>903_bases
GTGCGCGGCACGCGGCCCCGGAACTCGCGAACCATGACCCTGATTCGCCTCGGCGCATCGTGCGCCATCCTTGGCCTGCT
GGCCGCCTGCACGTCGCCGTCGCTGATCGAGCGCGGCAGCTACTACGCCGACACGGGCCTGCACGCGCGCGGCGCCGATG
CGCGGATCCGCTTTCTCGTGATGCATTACACCGAAAGCGACGAAGCGAAATCGCTGCGCACGCTGACCGGCGATTCGGTC
AGCGTCCACTACGTCGTGCCGCCGCAACCGCGCATCGAACACGGGATGCCGGTCGTCTATCAGCTCGTTCCTGAATCGAA
GCGCGCATGGCATGCGGGCATCAGCGAATGGCAGCGCACGACCGAGCTCAATGCGGCGTCGATCGGCATCGAGAACGTGA
ATCGCGGCCCGCTCGATCCGCAAAACCTTACCTGGCAGCCGTATTCGCCCGAGCAGGTCGATGCGCTGATTCGCCTGTCG
AAGGATATCGTCGCGCGCTACGGGATTGCGCCGACGCGCGTGGTCGGACACAGCGATATCGCACCGCAACGCAAGATCGA
TCCCGGTCCGCTGTTCCCGTGGCAGGCGCTCGCGCAGGCCGGCGTCGGCGCATGGCCGGACGATGCGACCGTGGCCGCGC
GGCTCGGCGATCGCGATCCGCACGCGCTCGTCGACGTGCGCGAATTGCAGCTGAAGCTCGCGCGCTACGGCTACGATGTG
CCGACCGACGGCGTGCTCGACGCGCGCACGCGCCGCGTATTCGCCGCATTCCAGATGCACTTCCGGCCATCCGACTACGC
GGGAAATCCGGACGCCGAGACCGACGCGATCGCGCAGGCGCTGCTCGACAAGTATTTCCCGGCTGCACCTGCCGACAACG
TGTCCGCCGACGGCACGCCGTGA

Upstream 100 bases:

>100_bases
GGCGCGTGAAGGGCTGATTCTCCGGCCGTCTCATAGGCTGACGCACGCGATCTCGCGCGATGCGCGGGCCGTCACGACCC
GAACGACGCGTGCCGCGATT

Downstream 100 bases:

>100_bases
CCGTCGGCTTGTCCTGGGCCGACATCCGCGCGGAAAAAGCCCGGGGCGTCTGGCGAGTCCGGTAAACTGGCGGTTTTCCG
CAATCCCGCTGCATTCCCAC

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRGTRPRNSRTMTLIRLGASCAILGLLAACTSPSLIERGSYYADTGLHARGADARIRFLVMHYTESDEAKSLRTLTGDSV
SVHYVVPPQPRIEHGMPVVYQLVPESKRAWHAGISEWQRTTELNAASIGIENVNRGPLDPQNLTWQPYSPEQVDALIRLS
KDIVARYGIAPTRVVGHSDIAPQRKIDPGPLFPWQALAQAGVGAWPDDATVAARLGDRDPHALVDVRELQLKLARYGYDV
PTDGVLDARTRRVFAAFQMHFRPSDYAGNPDAETDAIAQALLDKYFPAAPADNVSADGTP

Sequences:

>Translated_300_residues
MRGTRPRNSRTMTLIRLGASCAILGLLAACTSPSLIERGSYYADTGLHARGADARIRFLVMHYTESDEAKSLRTLTGDSV
SVHYVVPPQPRIEHGMPVVYQLVPESKRAWHAGISEWQRTTELNAASIGIENVNRGPLDPQNLTWQPYSPEQVDALIRLS
KDIVARYGIAPTRVVGHSDIAPQRKIDPGPLFPWQALAQAGVGAWPDDATVAARLGDRDPHALVDVRELQLKLARYGYDV
PTDGVLDARTRRVFAAFQMHFRPSDYAGNPDAETDAIAQALLDKYFPAAPADNVSADGTP
>Mature_300_residues
MRGTRPRNSRTMTLIRLGASCAILGLLAACTSPSLIERGSYYADTGLHARGADARIRFLVMHYTESDEAKSLRTLTGDSV
SVHYVVPPQPRIEHGMPVVYQLVPESKRAWHAGISEWQRTTELNAASIGIENVNRGPLDPQNLTWQPYSPEQVDALIRLS
KDIVARYGIAPTRVVGHSDIAPQRKIDPGPLFPWQALAQAGVGAWPDDATVAARLGDRDPHALVDVRELQLKLARYGYDV
PTDGVLDARTRRVFAAFQMHFRPSDYAGNPDAETDAIAQALLDKYFPAAPADNVSADGTP

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=252, Percent_Identity=54.3650793650794, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1786300, Length=96, Percent_Identity=38.5416666666667, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 32857; Mature: 32857

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGTRPRNSRTMTLIRLGASCAILGLLAACTSPSLIERGSYYADTGLHARGADARIRFLV
CCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCHHHHCCCEEECCCCCCCCCCCEEEEEE
MHYTESDEAKSLRTLTGDSVSVHYVVPPQPRIEHGMPVVYQLVPESKRAWHAGISEWQRT
EEECCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
TELNAASIGIENVNRGPLDPQNLTWQPYSPEQVDALIRLSKDIVARYGIAPTRVVGHSDI
HCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
APQRKIDPGPLFPWQALAQAGVGAWPDDATVAARLGDRDPHALVDVRELQLKLARYGYDV
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
PTDGVLDARTRRVFAAFQMHFRPSDYAGNPDAETDAIAQALLDKYFPAAPADNVSADGTP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRGTRPRNSRTMTLIRLGASCAILGLLAACTSPSLIERGSYYADTGLHARGADARIRFLV
CCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCHHHHCCCEEECCCCCCCCCCCEEEEEE
MHYTESDEAKSLRTLTGDSVSVHYVVPPQPRIEHGMPVVYQLVPESKRAWHAGISEWQRT
EEECCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHH
TELNAASIGIENVNRGPLDPQNLTWQPYSPEQVDALIRLSKDIVARYGIAPTRVVGHSDI
HCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
APQRKIDPGPLFPWQALAQAGVGAWPDDATVAARLGDRDPHALVDVRELQLKLARYGYDV
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
PTDGVLDARTRRVFAAFQMHFRPSDYAGNPDAETDAIAQALLDKYFPAAPADNVSADGTP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]