Click here to switch to the map view.

The map label for this gene is outE [H]

Identifier: 115351486

GI number: 115351486

Start: 1584364

End: 1585998

Strand: Direct

Name: outE [H]

Synonym: Bamb_1433

Alternate gene names: 115351486

Gene position: 1584364-1585998 (Clockwise)

Preceding gene: 115351485

Following gene: 115351487

Centisome position: 44.55

GC content: 68.81

Gene sequence:

>1635_bases
ATGAAAACGGAACACATCGTTCAACGCTGGCAGTCGGAATCCGAAGCATCGGGCGTTGGCTTCGCGCAATACGTGGAGGG
CCTGCTGGGCGCCGATCCGACGCGGCTCGCACAAGCGGCCGCCTGCCTTGGCATCGGCGCAGTCGACGCCGATACGCTGC
GCGCGGCGCCGACGCGCTTCGACCTGCTGCCGCTGCACGACGCGCTGACCTGGCGCGTGCTGCCGGTCGAGCTCGACGGC
GCGCCGTGCGTCGTCGTCGCCGATCCGTTCGCCCGCCACGTGCGCGACGAGCTGCTTGCGCGGTTCGGCGTGCGGCCGGT
CGAACTGGCCATGAGTGTGCCGGGCGAAGTCGAGCGGCTGCTCGGCGACATCGAGTCGAGCGAGCGGCTGATGGACGACG
TGACGATCGATACGCAGGGCGATTCGCTCGCACAGGCGATCGAGTCGATCTCGCTTGCGAGCATCGCGAAAGACGAGAGC
CCGATCATCAGGCTCGTCAACACGACGCTCTACGACGCGCTGCAGAGCCGCGCGAGCGATATTCATCTCGAAGCGGTGCC
GGACGGTCTGTCGATCCGCTACCGGATCGACGGCGTGCTGCAGTCGATCCGCCACATTCCCGGGCTCGAGGCGGCCGCGC
AGACGCTGTCGCGCCTGAAGGTGCTCGCCGCGCTCGACATCGCGGAGAAGCGCGTGCCGCAGGACGGCCGTTTCAAGGTC
GTGATGCAGGGCCGCGAGATCGATTTCCGCGTGTCGATCATTCCCGGCACCTATGGCGAAAACGCGGTGATGCGCGTGCT
CGACAAGTCGCAGCGCGGCGACAGCGTGAGCCTCGACGTGCTCGGCTTCGACGCGCAGACGGCGCGGCGCGTGCGTGCGC
TCGCGCACCGGCCTTACGGTCTGATGCTCGTGACGGGGCCGACCGGCAGCGGCAAGTCGACGACGCTGTACGCGGTGCTG
TCGGAAGTCAACACGGGCGACGACAAGATCATCACGATCGAGGACCCGGTCGAATACGAGCTGAAGGGCGTGCTGCAGAT
CCCGGTCAACGAGCGCAAGGGCCTCACGTTCGCGCGCGGGCTGCGCTCGATCCTGCGGCACGACCCGGACACGGTGATGG
TCGGCGAGATCCGCGACGAGGAGACCGCGTCGATCGCCGTGCAATCGGCGCTCACCGGCCACCGCGTGCTGTCGACCGTG
CATGCGAACGACGGGTTCAGCGTGATCGACCGCTTCATCTACATGGGCGTCGAGCCGTCGACGTTTCTCGAAGCGCTGAA
CGGCGTCGTGTCGCAGCGCCTGGTGCGGCGGATCTGCGCGCGCTGCGGCGGCACGGCGGCGGACGGCGCCGACCTGAGCG
GCGCGGCGCCCGACGAACTGAAGCGCGTGGTCACGCCCGGCACGGGCTGCGAGACCTGCCGCGGCACCGGCTATCACGGC
CGGATCGGACTCGCGGAAGTGCTGACGCTCGATGCGGCGATGAAGGCCGCGCTGCTCGAGCGCTCGATCGAGAAGCGTGC
GCAGGCCCTCGCCGGCAACGCCGACTACCGGTCGATGCGCGACGCGGGGCTTGCGGCGGTTCGCGCGCGGCTGACGACGT
TCCAGGAGGTGCAACGTGCGATCGCCGTGGAATAA

Upstream 100 bases:

>100_bases
GCTGCTTTACATGCCGATCTTCGAATTGGCGGGCAGCGTCGGATAGCGATCCGCACTGTCAACGCGTTCGCGATCCCGCT
GTTCAACAGGAGAAAACTCA

Downstream 100 bases:

>100_bases
CGTCATCGCGGTGCTCGACGCCGACACGCTGCGCGTGTGGACGAACGACGGCTGGCGCGCGCGGCGCGCCGGCGAGCCGC
ACGTCGTCGTCGCACACGAC

Product: type II secretion system protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MKTEHIVQRWQSESEASGVGFAQYVEGLLGADPTRLAQAAACLGIGAVDADTLRAAPTRFDLLPLHDALTWRVLPVELDG
APCVVVADPFARHVRDELLARFGVRPVELAMSVPGEVERLLGDIESSERLMDDVTIDTQGDSLAQAIESISLASIAKDES
PIIRLVNTTLYDALQSRASDIHLEAVPDGLSIRYRIDGVLQSIRHIPGLEAAAQTLSRLKVLAALDIAEKRVPQDGRFKV
VMQGREIDFRVSIIPGTYGENAVMRVLDKSQRGDSVSLDVLGFDAQTARRVRALAHRPYGLMLVTGPTGSGKSTTLYAVL
SEVNTGDDKIITIEDPVEYELKGVLQIPVNERKGLTFARGLRSILRHDPDTVMVGEIRDEETASIAVQSALTGHRVLSTV
HANDGFSVIDRFIYMGVEPSTFLEALNGVVSQRLVRRICARCGGTAADGADLSGAAPDELKRVVTPGTGCETCRGTGYHG
RIGLAEVLTLDAAMKAALLERSIEKRAQALAGNADYRSMRDAGLAAVRARLTTFQEVQRAIAVE

Sequences:

>Translated_544_residues
MKTEHIVQRWQSESEASGVGFAQYVEGLLGADPTRLAQAAACLGIGAVDADTLRAAPTRFDLLPLHDALTWRVLPVELDG
APCVVVADPFARHVRDELLARFGVRPVELAMSVPGEVERLLGDIESSERLMDDVTIDTQGDSLAQAIESISLASIAKDES
PIIRLVNTTLYDALQSRASDIHLEAVPDGLSIRYRIDGVLQSIRHIPGLEAAAQTLSRLKVLAALDIAEKRVPQDGRFKV
VMQGREIDFRVSIIPGTYGENAVMRVLDKSQRGDSVSLDVLGFDAQTARRVRALAHRPYGLMLVTGPTGSGKSTTLYAVL
SEVNTGDDKIITIEDPVEYELKGVLQIPVNERKGLTFARGLRSILRHDPDTVMVGEIRDEETASIAVQSALTGHRVLSTV
HANDGFSVIDRFIYMGVEPSTFLEALNGVVSQRLVRRICARCGGTAADGADLSGAAPDELKRVVTPGTGCETCRGTGYHG
RIGLAEVLTLDAAMKAALLERSIEKRAQALAGNADYRSMRDAGLAAVRARLTTFQEVQRAIAVE
>Mature_544_residues
MKTEHIVQRWQSESEASGVGFAQYVEGLLGADPTRLAQAAACLGIGAVDADTLRAAPTRFDLLPLHDALTWRVLPVELDG
APCVVVADPFARHVRDELLARFGVRPVELAMSVPGEVERLLGDIESSERLMDDVTIDTQGDSLAQAIESISLASIAKDES
PIIRLVNTTLYDALQSRASDIHLEAVPDGLSIRYRIDGVLQSIRHIPGLEAAAQTLSRLKVLAALDIAEKRVPQDGRFKV
VMQGREIDFRVSIIPGTYGENAVMRVLDKSQRGDSVSLDVLGFDAQTARRVRALAHRPYGLMLVTGPTGSGKSTTLYAVL
SEVNTGDDKIITIEDPVEYELKGVLQIPVNERKGLTFARGLRSILRHDPDTVMVGEIRDEETASIAVQSALTGHRVLSTV
HANDGFSVIDRFIYMGVEPSTFLEALNGVVSQRLVRRICARCGGTAADGADLSGAAPDELKRVVTPGTGCETCRGTGYHG
RIGLAEVLTLDAAMKAALLERSIEKRAQALAGNADYRSMRDAGLAAVRARLTTFQEVQRAIAVE

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=382, Percent_Identity=46.5968586387435, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1786296, Length=392, Percent_Identity=39.7959183673469, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI87082188, Length=191, Percent_Identity=37.17277486911, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 58717; Mature: 58717

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTEHIVQRWQSESEASGVGFAQYVEGLLGADPTRLAQAAACLGIGAVDADTLRAAPTRF
CCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCE
DLLPLHDALTWRVLPVELDGAPCVVVADPFARHVRDELLARFGVRPVELAMSVPGEVERL
EEEECCCCCEEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHH
LGDIESSERLMDDVTIDTQGDSLAQAIESISLASIAKDESPIIRLVNTTLYDALQSRASD
HHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
IHLEAVPDGLSIRYRIDGVLQSIRHIPGLEAAAQTLSRLKVLAALDIAEKRVPQDGRFKV
EEEEECCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VMQGREIDFRVSIIPGTYGENAVMRVLDKSQRGDSVSLDVLGFDAQTARRVRALAHRPYG
EEECCCEEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCE
LMLVTGPTGSGKSTTLYAVLSEVNTGDDKIITIEDPVEYELKGVLQIPVNERKGLTFARG
EEEEECCCCCCCCEEHEEEEHHCCCCCCEEEEECCCCCEEECEEEECCCCCCCCCHHHHH
LRSILRHDPDTVMVGEIRDEETASIAVQSALTGHRVLSTVHANDGFSVIDRFIYMGVEPS
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCHH
TFLEALNGVVSQRLVRRICARCGGTAADGADLSGAAPDELKRVVTPGTGCETCRGTGYHG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCC
RIGLAEVLTLDAAMKAALLERSIEKRAQALAGNADYRSMRDAGLAAVRARLTTFQEVQRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IAVE
HCCC
>Mature Secondary Structure
MKTEHIVQRWQSESEASGVGFAQYVEGLLGADPTRLAQAAACLGIGAVDADTLRAAPTRF
CCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCE
DLLPLHDALTWRVLPVELDGAPCVVVADPFARHVRDELLARFGVRPVELAMSVPGEVERL
EEEECCCCCEEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHH
LGDIESSERLMDDVTIDTQGDSLAQAIESISLASIAKDESPIIRLVNTTLYDALQSRASD
HHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
IHLEAVPDGLSIRYRIDGVLQSIRHIPGLEAAAQTLSRLKVLAALDIAEKRVPQDGRFKV
EEEEECCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VMQGREIDFRVSIIPGTYGENAVMRVLDKSQRGDSVSLDVLGFDAQTARRVRALAHRPYG
EEECCCEEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCE
LMLVTGPTGSGKSTTLYAVLSEVNTGDDKIITIEDPVEYELKGVLQIPVNERKGLTFARG
EEEEECCCCCCCCEEHEEEEHHCCCCCCEEEEECCCCCEEECEEEECCCCCCCCCHHHHH
LRSILRHDPDTVMVGEIRDEETASIAVQSALTGHRVLSTVHANDGFSVIDRFIYMGVEPS
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCHH
TFLEALNGVVSQRLVRRICARCGGTAADGADLSGAAPDELKRVVTPGTGCETCRGTGYHG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCC
RIGLAEVLTLDAAMKAALLERSIEKRAQALAGNADYRSMRDAGLAAVRARLTTFQEVQRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IAVE
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]