BacMap

Definition	Burkholderia ambifaria AMMD chromosome chromosome 1, complete sequence.
Accession	NC_008390
Length	3,556,545

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The map label for this gene is livG [H]

Identifier: 115351349

GI number: 115351349

Start: 1421163

End: 1421912

Strand: Direct

Name: livG [H]

Synonym: Bamb_1296

Alternate gene names: 115351349

Gene position: 1421163-1421912 (Clockwise)

Preceding gene: 115351348

Following gene: 115351350

Centisome position: 39.96

GC content: 73.47

Gene sequence:

>750_bases
ATGAACGGCAACGCGATCGCGCTGCACGGCATCGTGCAGCGCTTCGGCGCACAGACGGTGCTCGACGGTATCGAGCTGAG
CGTCTGCGCCGGCGAGCGTCACGCGCTGATCGGGCCGAACGGCGCGGGTAAGTCGACGCTGTTCGGCGTGATTGCCGGCG
CGACGCGGCCAACGCGCGGGCGCGTCGTGTTGCACGGTGTCGAGCTGCGCGGACGCGGGCCGGTCGTCGCGAGCCGGCTC
GGCATCGGCCGCAGTTTCCAGCAGACGAGCGCGTTCGCGCGGCTCTCCGTGTTCGACAACCTGCGCTGCGCGGCGCTGCA
TGCGCCGGACGAGCGGCGGCGCTGGTGGAACCGGCTGCGCGAATCGGCGTCGGTCGATCGCGCGGCCGCGCGCGTGCTGC
ACGACATCGGCCTCGATGCGCGGCGCGACGCGCCGGCCGGCGAACTCGGCTACGCGGAACAGCGCGCGCTCGATCTCGGC
ATCGCGCTCGCGAGCGGCGCGCGCACGCTGCTGCTCGACGAACCGACGGCCGGCATGAACCGCGACCAGGCGGCGCGGAT
GATCGCGCTGATCCGCGCGACGACGCAGGGCCGCACGGTGCTGATGATCGAGCACGACATGGATGCGGTGTTCGGCTTCG
CCGAGCGCGTCACGGTGCTGGTGCGCGGCGCGGTGGTCGCGACCGGTGCGCCCGACGCGATCCGCGCGGACCCGGCCGTG
CGCGCCGCCTATCTCGGCGACGTCGCATGA

Upstream 100 bases:

>100_bases
GCTGGGTCGTCGCGTGCGCGGCGGCCGGCGTCGGCATGCTGCTGTGGGGCTGGCGCGCGCGGCTGATGCACCCCGGGCAC
GGCAAGCGGGAGGACGGGCG

Downstream 100 bases:

>100_bases
GCGCGCTGCTCGACATTCGCGGCCTGCGCGCGTGGTACGGGAGGCAGCCCGTGCTCGACGGCGTCGATCTCGCGCTCGCG
CCGGGCGAGACGCTCGCGCT

Product: ABC transporter-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein G [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNGNAIALHGIVQRFGAQTVLDGIELSVCAGERHALIGPNGAGKSTLFGVIAGATRPTRGRVVLHGVELRGRGPVVASRL
GIGRSFQQTSAFARLSVFDNLRCAALHAPDERRRWWNRLRESASVDRAAARVLHDIGLDARRDAPAGELGYAEQRALDLG
IALASGARTLLLDEPTAGMNRDQAARMIALIRATTQGRTVLMIEHDMDAVFGFAERVTVLVRGAVVATGAPDAIRADPAV
RAAYLGDVA

Sequences:

>Translated_249_residues
MNGNAIALHGIVQRFGAQTVLDGIELSVCAGERHALIGPNGAGKSTLFGVIAGATRPTRGRVVLHGVELRGRGPVVASRL
GIGRSFQQTSAFARLSVFDNLRCAALHAPDERRRWWNRLRESASVDRAAARVLHDIGLDARRDAPAGELGYAEQRALDLG
IALASGARTLLLDEPTAGMNRDQAARMIALIRATTQGRTVLMIEHDMDAVFGFAERVTVLVRGAVVATGAPDAIRADPAV
RAAYLGDVA
>Mature_249_residues
MNGNAIALHGIVQRFGAQTVLDGIELSVCAGERHALIGPNGAGKSTLFGVIAGATRPTRGRVVLHGVELRGRGPVVASRL
GIGRSFQQTSAFARLSVFDNLRCAALHAPDERRRWWNRLRESASVDRAAARVLHDIGLDARRDAPAGELGYAEQRALDLG
IALASGARTLLLDEPTAGMNRDQAARMIALIRATTQGRTVLMIEHDMDAVFGFAERVTVLVRGAVVATGAPDAIRADPAV
RAAYLGDVA

Specific function: Component of the leucine-specific transport system [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27881501, Length=227, Percent_Identity=25.5506607929515, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI30795238, Length=227, Percent_Identity=25.5506607929515, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI150417984, Length=234, Percent_Identity=26.9230769230769, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI153792144, Length=232, Percent_Identity=27.1551724137931, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI31657092, Length=217, Percent_Identity=28.110599078341, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI45446740, Length=213, Percent_Identity=26.7605633802817, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI47078218, Length=213, Percent_Identity=26.7605633802817, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI105990541, Length=211, Percent_Identity=27.0142180094787, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI21536376, Length=222, Percent_Identity=25.6756756756757, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI116734710, Length=207, Percent_Identity=27.536231884058, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI27477115, Length=213, Percent_Identity=27.2300469483568, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI27262624, Length=232, Percent_Identity=27.5862068965517, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI27262626, Length=232, Percent_Identity=27.5862068965517, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI27436953, Length=209, Percent_Identity=29.1866028708134, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1789864, Length=261, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1790525, Length=245, Percent_Identity=28.1632653061224, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788210, Length=224, Percent_Identity=28.125, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082268, Length=247, Percent_Identity=31.5789473684211, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87081709, Length=248, Percent_Identity=25.4032258064516, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788761, Length=254, Percent_Identity=27.9527559055118, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI48995001, Length=219, Percent_Identity=28.7671232876712, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1789672, Length=235, Percent_Identity=27.2340425531915, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1789593, Length=236, Percent_Identity=30.5084745762712, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790190, Length=225, Percent_Identity=27.1111111111111, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788472, Length=230, Percent_Identity=25.2173913043478, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788225, Length=244, Percent_Identity=27.4590163934426, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787792, Length=233, Percent_Identity=27.0386266094421, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787029, Length=241, Percent_Identity=27.3858921161826, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI87081791, Length=250, Percent_Identity=25.6, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081782, Length=245, Percent_Identity=27.7551020408163, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786398, Length=243, Percent_Identity=27.5720164609054, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789991, Length=226, Percent_Identity=25.2212389380531, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788450, Length=242, Percent_Identity=27.6859504132231, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786872, Length=235, Percent_Identity=25.1063829787234, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1789032, Length=244, Percent_Identity=26.2295081967213, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790739, Length=250, Percent_Identity=27.2, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788897, Length=219, Percent_Identity=26.027397260274, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994943, Length=244, Percent_Identity=31.9672131147541, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787370, Length=251, Percent_Identity=24.7011952191235, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790544, Length=251, Percent_Identity=27.0916334661355, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786319, Length=234, Percent_Identity=26.9230769230769, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786253, Length=225, Percent_Identity=27.5555555555556, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787105, Length=211, Percent_Identity=27.9620853080569, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1790467, Length=248, Percent_Identity=28.2258064516129, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787089, Length=237, Percent_Identity=27.8481012658228, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1786703, Length=215, Percent_Identity=28.8372093023256, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787548, Length=233, Percent_Identity=27.4678111587983, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786563, Length=241, Percent_Identity=26.9709543568465, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787112, Length=214, Percent_Identity=28.9719626168224, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1789586, Length=230, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786803, Length=223, Percent_Identity=25.5605381165919, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081834, Length=216, Percent_Identity=25.462962962963, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788506, Length=236, Percent_Identity=27.1186440677966, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789873, Length=217, Percent_Identity=29.4930875576037, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87082267, Length=253, Percent_Identity=25.6916996047431, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788644, Length=249, Percent_Identity=23.6947791164659, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789891, Length=221, Percent_Identity=31.6742081447964, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1787712, Length=236, Percent_Identity=21.6101694915254, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786345, Length=234, Percent_Identity=24.7863247863248, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787164, Length=227, Percent_Identity=29.0748898678414, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=216, Percent_Identity=26.3888888888889, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17510237, Length=238, Percent_Identity=31.0924369747899, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71983523, Length=214, Percent_Identity=30.8411214953271, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71983516, Length=214, Percent_Identity=30.8411214953271, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71983510, Length=214, Percent_Identity=30.8411214953271, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI115533608, Length=202, Percent_Identity=30.1980198019802, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193202349, Length=223, Percent_Identity=25.5605381165919, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17565938, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543740, Length=218, Percent_Identity=23.8532110091743, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24643674, Length=219, Percent_Identity=25.1141552511416, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24666092, Length=228, Percent_Identity=25.4385964912281, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI221512771, Length=228, Percent_Identity=25.4385964912281, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648314, Length=234, Percent_Identity=23.9316239316239, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571209, Length=227, Percent_Identity=26.431718061674, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI116007184, Length=230, Percent_Identity=22.1739130434783, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221500365, Length=230, Percent_Identity=22.1739130434783, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26414; Mature: 26414

Theoretical pI: Translated: 10.90; Mature: 10.90

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGNAIALHGIVQRFGAQTVLDGIELSVCAGERHALIGPNGAGKSTLFGVIAGATRPTRG
CCCCEEHHHHHHHHHCHHHHHCCCEEEEECCCCEEEECCCCCCCHHHEEEEECCCCCCCC
RVVLHGVELRGRGPVVASRLGIGRSFQQTSAFARLSVFDNLRCAALHAPDERRRWWNRLR
EEEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHH
ESASVDRAAARVLHDIGLDARRDAPAGELGYAEQRALDLGIALASGARTLLLDEPTAGMN
HHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCEEEEEECCCCCCC
RDQAARMIALIRATTQGRTVLMIEHDMDAVFGFAERVTVLVRGAVVATGAPDAIRADPAV
HHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHH
RAAYLGDVA
HHHHHCCCC
>Mature Secondary Structure
MNGNAIALHGIVQRFGAQTVLDGIELSVCAGERHALIGPNGAGKSTLFGVIAGATRPTRG
CCCCEEHHHHHHHHHCHHHHHCCCEEEEECCCCEEEECCCCCCCHHHEEEEECCCCCCCC
RVVLHGVELRGRGPVVASRLGIGRSFQQTSAFARLSVFDNLRCAALHAPDERRRWWNRLR
EEEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHH
ESASVDRAAARVLHDIGLDARRDAPAGELGYAEQRALDLGIALASGARTLLLDEPTAGMN
HHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCEEEEEECCCCCCC
RDQAARMIALIRATTQGRTVLMIEHDMDAVFGFAERVTVLVRGAVVATGAPDAIRADPAV
HHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHH
RAAYLGDVA
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]