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The map label for this gene is nfdA [H]

Identifier: 115351326

GI number: 115351326

Start: 1393026

End: 1394918

Strand: Direct

Name: nfdA [H]

Synonym: Bamb_1273

Alternate gene names: 115351326

Gene position: 1393026-1394918 (Clockwise)

Preceding gene: 115351325

Following gene: 115351327

Centisome position: 39.17

GC content: 68.15

Gene sequence:

>1893_bases
ATGAGCGCAACCGATACCCAACCGGATCTGATCCTGCACAACGGGCGCATCACGACGCTCGATCGTGCGAACCCCGTCGC
CACCGCGGTGGCGATCAAGGACGGCCGCTTCGTCGCGGTCGGCAGCAACGCGGACGTGATGCCGCTCGCGGGCCGCGCAA
CGAAGGTCGTCGACCTCGACGGCCGCGCGGTGCTGCCGGGCCTGACCGACAACCATACGCACGTGATTCGCGGCGGCCTG
AACTACAACATGGAGCTGCGCTGGGACGGCGTGCGCTCGCTCGCCGTCGCGATGGAGATGCTGCGGCAGCAGGTCGCGAT
CACGCCCGCGCCGCAATGGGTGCGCGTGGTCGGCGGCTTCACCGAACATCAGTTTGCCGAGAAGCGCCTGCCGACGATCG
ACGAGCTCAATGCGGCCGCGCCCGATACGCCGGTGTTCATCCTGCACCTGTACGATCGCGCGCTGCTGAACGCGGCCGCG
CTGCGCGTCGTCGGCTATACGAAGGACACGCCGGAGCCGCCGGGCGGCACGATCCTGCGCGACGCCGCGGGCAACCCGAC
GGGCCTGCTGCTCGCGAACCCGAACGCGACGATCCTCTACGCGACGCTCGCGAAGGGTCCGAAGCTGCCGTTCGAGTACC
AGTACAACTCGACGCGCCACTTCATGCGCGAGCTGAACCGGCTCGGCGTGACGGGCGTGATCGACGCGGGCGGCGGCTCG
CAGAACTACCCCGACGATTACGAAGTGATCCGCCAGCTGCACGACGCGGGCGAGATGACGATCCGCATCGCGTACAACCT
GTTCACGCAGAAGCCGAACGCGGAGAAGGAAGACTTCGTGAACTGGACGAAGAGCGTCAAGTATCACGACGGCACCGACT
ACTTCCGCCACAACGGCGCGGGCGAGATGCTGGTGTTTTCCGCGGCCGACTTCGAGGACTTCCGCGTCGCGCGTCCGGAT
CTGCCCGCGCAGATGGAGGACGATCTCGAAGGCGTCGTGCGCGTGCTCGCGCAGAACCGCTGGCCGTGGCGCATGCATGC
GACCTACGACGAAACGATCAGCCGCGCGCTCGACGTGTTCGAGAAGGTCAACGAGGACATCCCGCTCGAAGGGCTGAACT
GGTTCTTCGATCACGCGGAAACGATCTCCGAGCGATCGATGGACCGGATCGCGGCGCTCGGCGGCGGCATCGCGGTGCAG
CACCGGATGGCGTATCAGGGCGAGTATTTCGTCGAGCGTTATGGCGCGCAGGCGGCCGAGGCGACGCCGCCGGTCGCGAA
GATGCTGTCCAAGGGCATCAAGGTGTCGGCCGGCACGGACGCGACGCGGGTGGCGTCGTACAACCCGTGGGTGTCGCTCG
CGTGGCTCGTGACGGGCAAGACGGTCGGCGGCACGCGCCTGTATCCGCAGCGCAACCTGCTCGATCGCGAAACCGCGCTG
CGCATGTGGACCGAGTACGTGACGTGGTTCTCGAACGAAGTGGGCAAGAAGGGCCGCATCGCGGTCGGGCAGTTGGCAGA
CCTGATGGTCCCCGACCGCGATTTCTTCACGTGCGCGGAGGACGATATCGCCGACACGACCGCGTTGCTGACGGTGGTCG
GCGGGCGGATCGTGTGGGGTGCCGGGCCGTTCGCGTCGCACGATATGCCGATTCCGCCGGCGATGCCGGACTGGTCGCCG
GTGCGCGCGTACGGCGGCTATGGCGGCTGGGGCGCGACGCAGCGCGAGGGCGCGCCGCTGCAGCGCGCAGCAGCGGCCGC
TGCCGCGTGCGGTTGTTCGACCGCGTGCGGGATGCACGGCCACGCACATGCGAACGCATGGGGCCGCACGTTGCCGACGT
CGGACGCGAAGGGCTTCTGGGGCGCGTTCGGCTGTTCGTGCTGGGCGGTCTGA

Upstream 100 bases:

>100_bases
CTGATCGCACTGATCGGCCTTGCCGGCATGCTGGTCGGCGCGCAGGCCATCGGGCCCGTGCGTCACTGGCTCGGCTTCTG
ACTGTTTCGAGGACATGTTA

Downstream 100 bases:

>100_bases
CATGACGACCCTGACCGGCCGATGTAGCCCCGGGTGGATTCGCGCGCTTCTTTCCCAGCCATGGGTCGGCGCGCTGGTTC
GGCTCGCGCTCGTTTCCGCG

Product: amidohydrolase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MSATDTQPDLILHNGRITTLDRANPVATAVAIKDGRFVAVGSNADVMPLAGRATKVVDLDGRAVLPGLTDNHTHVIRGGL
NYNMELRWDGVRSLAVAMEMLRQQVAITPAPQWVRVVGGFTEHQFAEKRLPTIDELNAAAPDTPVFILHLYDRALLNAAA
LRVVGYTKDTPEPPGGTILRDAAGNPTGLLLANPNATILYATLAKGPKLPFEYQYNSTRHFMRELNRLGVTGVIDAGGGS
QNYPDDYEVIRQLHDAGEMTIRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRHNGAGEMLVFSAADFEDFRVARPD
LPAQMEDDLEGVVRVLAQNRWPWRMHATYDETISRALDVFEKVNEDIPLEGLNWFFDHAETISERSMDRIAALGGGIAVQ
HRMAYQGEYFVERYGAQAAEATPPVAKMLSKGIKVSAGTDATRVASYNPWVSLAWLVTGKTVGGTRLYPQRNLLDRETAL
RMWTEYVTWFSNEVGKKGRIAVGQLADLMVPDRDFFTCAEDDIADTTALLTVVGGRIVWGAGPFASHDMPIPPAMPDWSP
VRAYGGYGGWGATQREGAPLQRAAAAAAACGCSTACGMHGHAHANAWGRTLPTSDAKGFWGAFGCSCWAV

Sequences:

>Translated_630_residues
MSATDTQPDLILHNGRITTLDRANPVATAVAIKDGRFVAVGSNADVMPLAGRATKVVDLDGRAVLPGLTDNHTHVIRGGL
NYNMELRWDGVRSLAVAMEMLRQQVAITPAPQWVRVVGGFTEHQFAEKRLPTIDELNAAAPDTPVFILHLYDRALLNAAA
LRVVGYTKDTPEPPGGTILRDAAGNPTGLLLANPNATILYATLAKGPKLPFEYQYNSTRHFMRELNRLGVTGVIDAGGGS
QNYPDDYEVIRQLHDAGEMTIRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRHNGAGEMLVFSAADFEDFRVARPD
LPAQMEDDLEGVVRVLAQNRWPWRMHATYDETISRALDVFEKVNEDIPLEGLNWFFDHAETISERSMDRIAALGGGIAVQ
HRMAYQGEYFVERYGAQAAEATPPVAKMLSKGIKVSAGTDATRVASYNPWVSLAWLVTGKTVGGTRLYPQRNLLDRETAL
RMWTEYVTWFSNEVGKKGRIAVGQLADLMVPDRDFFTCAEDDIADTTALLTVVGGRIVWGAGPFASHDMPIPPAMPDWSP
VRAYGGYGGWGATQREGAPLQRAAAAAAACGCSTACGMHGHAHANAWGRTLPTSDAKGFWGAFGCSCWAV
>Mature_629_residues
SATDTQPDLILHNGRITTLDRANPVATAVAIKDGRFVAVGSNADVMPLAGRATKVVDLDGRAVLPGLTDNHTHVIRGGLN
YNMELRWDGVRSLAVAMEMLRQQVAITPAPQWVRVVGGFTEHQFAEKRLPTIDELNAAAPDTPVFILHLYDRALLNAAAL
RVVGYTKDTPEPPGGTILRDAAGNPTGLLLANPNATILYATLAKGPKLPFEYQYNSTRHFMRELNRLGVTGVIDAGGGSQ
NYPDDYEVIRQLHDAGEMTIRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRHNGAGEMLVFSAADFEDFRVARPDL
PAQMEDDLEGVVRVLAQNRWPWRMHATYDETISRALDVFEKVNEDIPLEGLNWFFDHAETISERSMDRIAALGGGIAVQH
RMAYQGEYFVERYGAQAAEATPPVAKMLSKGIKVSAGTDATRVASYNPWVSLAWLVTGKTVGGTRLYPQRNLLDRETALR
MWTEYVTWFSNEVGKKGRIAVGQLADLMVPDRDFFTCAEDDIADTTALLTVVGGRIVWGAGPFASHDMPIPPAMPDWSPV
RAYGGYGGWGATQREGAPLQRAAAAAAACGCSTACGMHGHAHANAWGRTLPTSDAKGFWGAFGCSCWAV

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 68912; Mature: 68780

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATDTQPDLILHNGRITTLDRANPVATAVAIKDGRFVAVGSNADVMPLAGRATKVVDLD
CCCCCCCCCEEEECCEEEEECCCCCEEEEEEEECCEEEEECCCCCEEEECCCCEEEEECC
GRAVLPGLTDNHTHVIRGGLNYNMELRWDGVRSLAVAMEMLRQQVAITPAPQWVRVVGGF
CCEECCCCCCCCCEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
TEHQFAEKRLPTIDELNAAAPDTPVFILHLYDRALLNAAALRVVGYTKDTPEPPGGTILR
HHHHHHHHCCCCHHHCCCCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCCCCCCCCCEEE
DAAGNPTGLLLANPNATILYATLAKGPKLPFEYQYNSTRHFMRELNRLGVTGVIDAGGGS
CCCCCCCEEEEECCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHCCCEEEEECCCCC
QNYPDDYEVIRQLHDAGEMTIRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRHNGA
CCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHCEEECCCCHHHHCCCC
GEMLVFSAADFEDFRVARPDLPAQMEDDLEGVVRVLAQNRWPWRMHATYDETISRALDVF
CCEEEEEECCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
EKVNEDIPLEGLNWFFDHAETISERSMDRIAALGGGIAVQHRMAYQGEYFVERYGAQAAE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHCCCCCC
ATPPVAKMLSKGIKVSAGTDATRVASYNPWVSLAWLVTGKTVGGTRLYPQRNLLDRETAL
CCCHHHHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHH
RMWTEYVTWFSNEVGKKGRIAVGQLADLMVPDRDFFTCAEDDIADTTALLTVVGGRIVWG
HHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCEEEEE
AGPFASHDMPIPPAMPDWSPVRAYGGYGGWGATQREGAPLQRAAAAAAACGCSTACGMHG
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCC
HAHANAWGRTLPTSDAKGFWGAFGCSCWAV
CCCCCCCCCCCCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
SATDTQPDLILHNGRITTLDRANPVATAVAIKDGRFVAVGSNADVMPLAGRATKVVDLD
CCCCCCCCEEEECCEEEEECCCCCEEEEEEEECCEEEEECCCCCEEEECCCCEEEEECC
GRAVLPGLTDNHTHVIRGGLNYNMELRWDGVRSLAVAMEMLRQQVAITPAPQWVRVVGGF
CCEECCCCCCCCCEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
TEHQFAEKRLPTIDELNAAAPDTPVFILHLYDRALLNAAALRVVGYTKDTPEPPGGTILR
HHHHHHHHCCCCHHHCCCCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCCCCCCCCCEEE
DAAGNPTGLLLANPNATILYATLAKGPKLPFEYQYNSTRHFMRELNRLGVTGVIDAGGGS
CCCCCCCEEEEECCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHCCCEEEEECCCCC
QNYPDDYEVIRQLHDAGEMTIRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRHNGA
CCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHCEEECCCCHHHHCCCC
GEMLVFSAADFEDFRVARPDLPAQMEDDLEGVVRVLAQNRWPWRMHATYDETISRALDVF
CCEEEEEECCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
EKVNEDIPLEGLNWFFDHAETISERSMDRIAALGGGIAVQHRMAYQGEYFVERYGAQAAE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHCCCCCC
ATPPVAKMLSKGIKVSAGTDATRVASYNPWVSLAWLVTGKTVGGTRLYPQRNLLDRETAL
CCCHHHHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHH
RMWTEYVTWFSNEVGKKGRIAVGQLADLMVPDRDFFTCAEDDIADTTALLTVVGGRIVWG
HHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCEEEEE
AGPFASHDMPIPPAMPDWSPVRAYGGYGGWGATQREGAPLQRAAAAAAACGCSTACGMHG
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCC
HAHANAWGRTLPTSDAKGFWGAFGCSCWAV
CCCCCCCCCCCCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA