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The map label for this gene is argT [H]

Identifier: 115351121

GI number: 115351121

Start: 1159561

End: 1160355

Strand: Direct

Name: argT [H]

Synonym: Bamb_1067

Alternate gene names: 115351121

Gene position: 1159561-1160355 (Clockwise)

Preceding gene: 115351120

Following gene: 115351124

Centisome position: 32.6

GC content: 65.91

Gene sequence:

>795_bases
ATGAAGTTGGATTGGCGTAAAGTGGCCGCGCACGCGGTAGTGGCGGCAACGGCCGTGGCGGCAGGCAGCGCGATGGCGGC
GGATCTGAAGGAGATCCGGTTCGGCGTGGAGGCCTCGTACGCGCCGTTCGAATACAAGACGCCCGACGGCAAGCTGACCG
GCTTCGACATCGACATCGGCAACGCGGTGTGCGCGAAGCTGAAGGTGAAGTGCGTGTGGGTCGAGAACGACTTCGACGGC
CTGATCCCCGCGCTGCAGGCGCGCAAGTTCGATGCGATCAACTCGGACATGACGATCACCGACCAGCGCAAGCACGCGAT
CGCGTTCACCGATCCGATCTACACGATCCCGAACCAGCTGATCGCGAAGCAGGGCAGCGGCCTCCAGCCGACGCCGCAAG
CGCTGAAGGGCAAGCGGGTCGGCGTGCTGCAGGGCACGATCCAGGAAGCGTACGCGAAGAAGAAGTGGGCGCCGGCCGGT
GTCGAGGTCGTGCCGTACCAGACGCAGGACCTGGCCTACGCCGACCTGAAGTCGGGCCGTCTCGACGCGACGTTCCAGGA
TTCGGAAGCGGGCTCGAAGGGCTTCCTGTCGAAGCCGCAAGGCCAGGGCTTCGGCTTCGCCGGCGGCACGGTGGCCGATG
CCGACATCCTCGGCTCGGGCGTCGGCTTCGGCCTGCGCAAGAACGACGCGGCGCTGAAGACCGCGCTCGATCAGGCGCTG
AAGGGGCTGAAGGCCGACGGCACGATCGACAACCTCGCGAAGAAGTACTTCAGCGTGCCGGTCACGCTCAAGTAA

Upstream 100 bases:

>100_bases
CGCATCAAGGCGCGGCATCGGGATTGTCGTGGTCATCCGGCCCGGCAATGATGCAAGCGCCCGTAGTTCCGGAACATTCG
AGTATCGAGGAGAACACCTG

Downstream 100 bases:

>100_bases
GCGTTTCGATCCGTCGCACGATACAGCCGGCCGCTTTCGCGGCCGGTTTGCTTTTGGGGCGTCGCTTCTTGCGCACGGGG
CGGCGTACCGCGTGCGTCAC

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKLDWRKVAAHAVVAATAVAAGSAMAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIGNAVCAKLKVKCVWVENDFDG
LIPALQARKFDAINSDMTITDQRKHAIAFTDPIYTIPNQLIAKQGSGLQPTPQALKGKRVGVLQGTIQEAYAKKKWAPAG
VEVVPYQTQDLAYADLKSGRLDATFQDSEAGSKGFLSKPQGQGFGFAGGTVADADILGSGVGFGLRKNDAALKTALDQAL
KGLKADGTIDNLAKKYFSVPVTLK

Sequences:

>Translated_264_residues
MKLDWRKVAAHAVVAATAVAAGSAMAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIGNAVCAKLKVKCVWVENDFDG
LIPALQARKFDAINSDMTITDQRKHAIAFTDPIYTIPNQLIAKQGSGLQPTPQALKGKRVGVLQGTIQEAYAKKKWAPAG
VEVVPYQTQDLAYADLKSGRLDATFQDSEAGSKGFLSKPQGQGFGFAGGTVADADILGSGVGFGLRKNDAALKTALDQAL
KGLKADGTIDNLAKKYFSVPVTLK
>Mature_264_residues
MKLDWRKVAAHAVVAATAVAAGSAMAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIGNAVCAKLKVKCVWVENDFDG
LIPALQARKFDAINSDMTITDQRKHAIAFTDPIYTIPNQLIAKQGSGLQPTPQALKGKRVGVLQGTIQEAYAKKKWAPAG
VEVVPYQTQDLAYADLKSGRLDATFQDSEAGSKGFLSKPQGQGFGFAGGTVADADILGSGVGFGLRKNDAALKTALDQAL
KGLKADGTIDNLAKKYFSVPVTLK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=241, Percent_Identity=52.2821576763486, Blast_Score=261, Evalue=2e-71,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=49.1379310344828, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=35.1020408163265, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1787088, Length=252, Percent_Identity=32.1428571428571, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1788228, Length=262, Percent_Identity=31.2977099236641, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1787031, Length=252, Percent_Identity=31.7460317460317, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28040; Mature: 28040

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDWRKVAAHAVVAATAVAAGSAMAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIG
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEECC
NAVCAKLKVKCVWVENDFDGLIPALQARKFDAINSDMTITDQRKHAIAFTDPIYTIPNQL
CCEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCEEEEECCCHHHCCHHH
IAKQGSGLQPTPQALKGKRVGVLQGTIQEAYAKKKWAPAGVEVVPYQTQDLAYADLKSGR
HHCCCCCCCCCHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHCCCCC
LDATFQDSEAGSKGFLSKPQGQGFGFAGGTVADADILGSGVGFGLRKNDAALKTALDQAL
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHH
KGLKADGTIDNLAKKYFSVPVTLK
HCCCCCCCHHHHHHHHHCCCCEEC
>Mature Secondary Structure
MKLDWRKVAAHAVVAATAVAAGSAMAADLKEIRFGVEASYAPFEYKTPDGKLTGFDIDIG
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEECC
NAVCAKLKVKCVWVENDFDGLIPALQARKFDAINSDMTITDQRKHAIAFTDPIYTIPNQL
CCEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCEEEEECCCHHHCCHHH
IAKQGSGLQPTPQALKGKRVGVLQGTIQEAYAKKKWAPAGVEVVPYQTQDLAYADLKSGR
HHCCCCCCCCCHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHCCCCC
LDATFQDSEAGSKGFLSKPQGQGFGFAGGTVADADILGSGVGFGLRKNDAALKTALDQAL
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHH
KGLKADGTIDNLAKKYFSVPVTLK
HCCCCCCCHHHHHHHHHCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]