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The map label for this gene is otsB [H]

Identifier: 115351089

GI number: 115351089

Start: 1122682

End: 1123434

Strand: Direct

Name: otsB [H]

Synonym: Bamb_1035

Alternate gene names: 115351089

Gene position: 1122682-1123434 (Clockwise)

Preceding gene: 115351085

Following gene: 115351090

Centisome position: 31.57

GC content: 69.85

Gene sequence:

>753_bases
ATGCAATCCGTTCCGGCTTCCCTGTCCCTGACCGATACCGCGTTCTTTTTCGACTTCGACGGTACGCTCGTCGAACTGGC
GCCGACGCCCGACAGCATTCACGTGCCGCCGTCGCTGCTGACGCTGCTCGATGAGCTGCGGCGCCGCTCGCATGGCGCGC
TCGCCGTCGTGTCGGGCCGCGGCATCGACAACCTCGACGCGTTCCTGAACATGCCGGACCTGCCGATCGCCGGCCTGCAC
GGCGCCGAGCGTCGCGACGCGAACGGCGACACGCAGCGCATCGGCTTCAACGACGACCGGCTGTTGCGCATCGAGCGCGA
ACTCGCGTCCGTCGTCGACCGTCATCCGGGCATGCTGCTCGAAATCAAGGGAGCGGCCGTCGCGCTGCACTACCGCAATG
CGCCCGAGCGCGAGCCGGCCGCGCGCGAAGCGGCCGAGCGCCTCGTCGCCGAATATGCGGACGCGTATGTGCTGCAGCCG
GGCAAGATGGTGTTCGAGATCAAGCCGAAGGGTGTCGACAAGGGGCGCGCGCTGGCTGCGTTCCTCGACGAGCCGCCGTT
CGCGGGCCGCGTGCCGCTGTTCGCGGGCGACGACCTGACCGACGAGAAGGGCTTCGCGGTGGTCAACGCGCGGGGCGGTC
TGTCGATCAAGGTCGGCGCGGGCGAGACGTCGGCCCGCATGCGGCTCGACTCGGTCGACGCGCTGCACGCGCAGATTGCC
CGCTGGCTTGGCGCGGAGCAGCGGGGCGCATGA

Upstream 100 bases:

>100_bases
TTTCGCCCATCTCGGTACGCAGGCGCGGTAAGATGCGCACAATTCCATTCTCGCAGCGGCGGCCGCGCGTGTGCAGGCCG
CTCCTCAGACCATCGAATCG

Downstream 100 bases:

>100_bases
GTCGACTCATCATCGTTTCGAACCGCGTCGCCCCGATTTCGGAAGGTGAACCGGCGGCGGGTGGCCTCGCGATCGGCGTC
TACGATGCGCTGAAGGAGAC

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MQSVPASLSLTDTAFFFDFDGTLVELAPTPDSIHVPPSLLTLLDELRRRSHGALAVVSGRGIDNLDAFLNMPDLPIAGLH
GAERRDANGDTQRIGFNDDRLLRIERELASVVDRHPGMLLEIKGAAVALHYRNAPEREPAAREAAERLVAEYADAYVLQP
GKMVFEIKPKGVDKGRALAAFLDEPPFAGRVPLFAGDDLTDEKGFAVVNARGGLSIKVGAGETSARMRLDSVDALHAQIA
RWLGAEQRGA

Sequences:

>Translated_250_residues
MQSVPASLSLTDTAFFFDFDGTLVELAPTPDSIHVPPSLLTLLDELRRRSHGALAVVSGRGIDNLDAFLNMPDLPIAGLH
GAERRDANGDTQRIGFNDDRLLRIERELASVVDRHPGMLLEIKGAAVALHYRNAPEREPAAREAAERLVAEYADAYVLQP
GKMVFEIKPKGVDKGRALAAFLDEPPFAGRVPLFAGDDLTDEKGFAVVNARGGLSIKVGAGETSARMRLDSVDALHAQIA
RWLGAEQRGA
>Mature_250_residues
MQSVPASLSLTDTAFFFDFDGTLVELAPTPDSIHVPPSLLTLLDELRRRSHGALAVVSGRGIDNLDAFLNMPDLPIAGLH
GAERRDANGDTQRIGFNDDRLLRIERELASVVDRHPGMLLEIKGAAVALHYRNAPEREPAAREAAERLVAEYADAYVLQP
GKMVFEIKPKGVDKGRALAAFLDEPPFAGRVPLFAGDDLTDEKGFAVVNARGGLSIKVGAGETSARMRLDSVDALHAQIA
RWLGAEQRGA

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=219, Percent_Identity=50.2283105022831, Blast_Score=208, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6320279, Length=223, Percent_Identity=27.3542600896861, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI221473368, Length=251, Percent_Identity=27.4900398406374, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI20129307, Length=251, Percent_Identity=27.4900398406374, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24582490, Length=251, Percent_Identity=27.4900398406374, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19920676, Length=244, Percent_Identity=27.0491803278689, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 26998; Mature: 26998

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSVPASLSLTDTAFFFDFDGTLVELAPTPDSIHVPPSLLTLLDELRRRSHGALAVVSGR
CCCCCCCEEEECEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
GIDNLDAFLNMPDLPIAGLHGAERRDANGDTQRIGFNDDRLLRIERELASVVDRHPGMLL
CCCCHHHHHCCCCCCEECCCCCCCCCCCCCCEEECCCCCCEEEHHHHHHHHHHCCCCEEE
EIKGAAVALHYRNAPEREPAAREAAERLVAEYADAYVLQPGKMVFEIKPKGVDKGRALAA
EECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCEEEE
FLDEPPFAGRVPLFAGDDLTDEKGFAVVNARGGLSIKVGAGETSARMRLDSVDALHAQIA
ECCCCCCCCCCCEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHHHHH
RWLGAEQRGA
HHHCCCCCCC
>Mature Secondary Structure
MQSVPASLSLTDTAFFFDFDGTLVELAPTPDSIHVPPSLLTLLDELRRRSHGALAVVSGR
CCCCCCCEEEECEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
GIDNLDAFLNMPDLPIAGLHGAERRDANGDTQRIGFNDDRLLRIERELASVVDRHPGMLL
CCCCHHHHHCCCCCCEECCCCCCCCCCCCCCEEECCCCCCEEEHHHHHHHHHHCCCCEEE
EIKGAAVALHYRNAPEREPAAREAAERLVAEYADAYVLQPGKMVFEIKPKGVDKGRALAA
EECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCEEEE
FLDEPPFAGRVPLFAGDDLTDEKGFAVVNARGGLSIKVGAGETSARMRLDSVDALHAQIA
ECCCCCCCCCCCEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHHHHH
RWLGAEQRGA
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]