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The map label for this gene is rfbE [H]

Identifier: 115350799

GI number: 115350799

Start: 824542

End: 825291

Strand: Direct

Name: rfbE [H]

Synonym: Bamb_0745

Alternate gene names: 115350799

Gene position: 824542-825291 (Clockwise)

Preceding gene: 115350798

Following gene: 115350800

Centisome position: 23.18

GC content: 60.8

Gene sequence:

>750_bases
ATGGCGTTTATCGAATTGCAGAGCGTAACGCTCGATTTGCCGATTTTCGATGTGCAGGGGCGCTCGCTCAAGAAGCAGGT
GCTCAACATGGGGCGTCGCAACAGGATCGCCGAGGGCAACGACGGCGTGATCGTCGTGCGCGCGCTCGACGACGTGAGTT
TTCGCTTCGAGCGTGGCGATCGCGTGGGCCTGATCGGGCACAACGGGGCGGGGAAATCGACATTGCTTCGTGCGATGGCG
GGCATCTATCCGCCGTCGGCGGGCCAGTTGTCGCGTGAAGGAAAAGTGGTGCCGCTCCTCGATATCGGCCTCGGTATGGA
CGAGAACTCGACCGGCATGCAGAACATCCGGCTCAGAGGCCTGCTGCTCGGCATGTCCGATGCGGAGATTCGTTCGAAAC
AGCGCGACATCGCGGAATTCTGCGAACTCGGCGATTATCTGGACCTTCCGATCCGCACGTACTCCAGCGGGATGAAGGTG
CGGCTCGCGTTTGCAGTATCGACGGCGGTCGATGCCGAAATCCTGTTGCTCGACGAGGTAATGGGAGTCGGCGACGCATC
GTTCATGCACAAGGCCGAAGCGCGATTGGCGGACCTGCACAGCCGTGCGGAAATCGTCGTGCTGGCGATGCACTCGAACT
CCGAAATCCGAAAGGTGTGCAACAAGGTCCTTTGGATGGAGCGTGGCCGTGTGCGCGCTTTCGGGCCGACGGAAGAGGTC
GTGTCGGCTTATGAGGCGACAACAGGCTGA

Upstream 100 bases:

>100_bases
GGCGTGACGATTGGCATGGCGGCGATCGGTTGGCCTGTCGCCATGTTGATGACCGGGCGTTATCTGAAGCGCATTCCGTA
CTGGGTCTGAGAGAGACGAT

Downstream 100 bases:

>100_bases
GCGCGTCGACCGGCCAGGCCGTGCGTGCGTGATCGCGGCGGCATACGACGGCACGATGTCCGGTGACGAGCCGCTCCTGG
CGGAGTGCGATCGGCTCCCT

Product: ABC transporter-like protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MAFIELQSVTLDLPIFDVQGRSLKKQVLNMGRRNRIAEGNDGVIVVRALDDVSFRFERGDRVGLIGHNGAGKSTLLRAMA
GIYPPSAGQLSREGKVVPLLDIGLGMDENSTGMQNIRLRGLLLGMSDAEIRSKQRDIAEFCELGDYLDLPIRTYSSGMKV
RLAFAVSTAVDAEILLLDEVMGVGDASFMHKAEARLADLHSRAEIVVLAMHSNSEIRKVCNKVLWMERGRVRAFGPTEEV
VSAYEATTG

Sequences:

>Translated_249_residues
MAFIELQSVTLDLPIFDVQGRSLKKQVLNMGRRNRIAEGNDGVIVVRALDDVSFRFERGDRVGLIGHNGAGKSTLLRAMA
GIYPPSAGQLSREGKVVPLLDIGLGMDENSTGMQNIRLRGLLLGMSDAEIRSKQRDIAEFCELGDYLDLPIRTYSSGMKV
RLAFAVSTAVDAEILLLDEVMGVGDASFMHKAEARLADLHSRAEIVVLAMHSNSEIRKVCNKVLWMERGRVRAFGPTEEV
VSAYEATTG
>Mature_248_residues
AFIELQSVTLDLPIFDVQGRSLKKQVLNMGRRNRIAEGNDGVIVVRALDDVSFRFERGDRVGLIGHNGAGKSTLLRAMAG
IYPPSAGQLSREGKVVPLLDIGLGMDENSTGMQNIRLRGLLLGMSDAEIRSKQRDIAEFCELGDYLDLPIRTYSSGMKVR
LAFAVSTAVDAEILLLDEVMGVGDASFMHKAEARLADLHSRAEIVVLAMHSNSEIRKVCNKVLWMERGRVRAFGPTEEVV
SAYEATTG

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbD [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27477115, Length=229, Percent_Identity=28.3842794759825, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI27436953, Length=199, Percent_Identity=27.1356783919598, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI6005701, Length=221, Percent_Identity=28.9592760180996, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI153792144, Length=211, Percent_Identity=26.0663507109005, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI27262624, Length=206, Percent_Identity=25.2427184466019, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI27262626, Length=206, Percent_Identity=25.2427184466019, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI87081791, Length=213, Percent_Identity=30.0469483568075, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI48994943, Length=206, Percent_Identity=28.1553398058252, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87081709, Length=203, Percent_Identity=25.615763546798, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788761, Length=201, Percent_Identity=27.363184079602, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790467, Length=196, Percent_Identity=25.5102040816327, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786398, Length=219, Percent_Identity=25.1141552511416, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17569143, Length=215, Percent_Identity=29.3023255813954, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=217, Percent_Identity=24.4239631336406, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=211, Percent_Identity=25.1184834123223, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI221500365, Length=186, Percent_Identity=31.1827956989247, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI116007184, Length=186, Percent_Identity=31.1827956989247, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648314, Length=217, Percent_Identity=28.110599078341, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24666092, Length=204, Percent_Identity=25, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27279; Mature: 27148

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFIELQSVTLDLPIFDVQGRSLKKQVLNMGRRNRIAEGNDGVIVVRALDDVSFRFERGD
CCEEEEEEEEEECEEEECCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCEEEECCCC
RVGLIGHNGAGKSTLLRAMAGIYPPSAGQLSREGKVVPLLDIGLGMDENSTGMQNIRLRG
EEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHEEEEE
LLLGMSDAEIRSKQRDIAEFCELGDYLDLPIRTYSSGMKVRLAFAVSTAVDAEILLLDEV
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEEEEEECCCCCEEEEEHHH
MGVGDASFMHKAEARLADLHSRAEIVVLAMHSNSEIRKVCNKVLWMERGRVRAFGPTEEV
HCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCHHHH
VSAYEATTG
HHHHHCCCC
>Mature Secondary Structure 
AFIELQSVTLDLPIFDVQGRSLKKQVLNMGRRNRIAEGNDGVIVVRALDDVSFRFERGD
CEEEEEEEEEECEEEECCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCEEEECCCC
RVGLIGHNGAGKSTLLRAMAGIYPPSAGQLSREGKVVPLLDIGLGMDENSTGMQNIRLRG
EEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHEEEEE
LLLGMSDAEIRSKQRDIAEFCELGDYLDLPIRTYSSGMKVRLAFAVSTAVDAEILLLDEV
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEEEEEECCCCCEEEEEHHH
MGVGDASFMHKAEARLADLHSRAEIVVLAMHSNSEIRKVCNKVLWMERGRVRAFGPTEEV
HCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCHHHH
VSAYEATTG
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]