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The map label for this gene is citH [H]

Identifier: 115350662

GI number: 115350662

Start: 679362

End: 680639

Strand: Direct

Name: citH [H]

Synonym: Bamb_0608

Alternate gene names: 115350662

Gene position: 679362-680639 (Clockwise)

Preceding gene: 115350661

Following gene: 115350668

Centisome position: 19.1

GC content: 68.86

Gene sequence:

>1278_bases
ATGCACGCCGTCACGCAGTCCAGCTCCATTGCCTCGTCGCCGCGCGTGTGGCGCGCGGTGGTTGCCGCGTCGATCGGCAA
CGCGCTCGAGTGGTTCGACCTCGTCGTCTACGGCTTCTTCGCGGTGACGATCTCGAAGCTGTTCTTTCCGGCCGGCAACG
ACACCGTGTCGCTGCTGCTCACGCTCGGCACGTTCGGCGTGTCCTTCTTCATGCGTCCGCTCGGCGCGATCGTGCTCGGC
GCGTACGCCGACCGCGCGGGCCGCAAGGGCGCGCTCACGCTGTCGATCCTGCTGATGATGGCCGGCACGCTCGTCATCGC
GGTGCTGCCGACCTACGGGACGATCGGCGTCGCGGCGCCGCTGATCCTCGTCGCCGCACGGCTGATGCAGGGTTTCTCGG
CGGGCGGCGAATTCGGCAGCGCGACCGCGTTCCTGGCCGAGCACGTGCCGGGCCGGCGCGGCTTCTTCGCGAGCTGGCAG
GTCGCGAGCCAGGGGCTCACGACCCTGCTTGCCGCCGGTTTCGGCACCGTGCTGAACGCGCAGCTCACGGCCGAGCAGAT
GGCCGCGTGGGGCTGGCGCATTCCGTTCTTCTTCGGGCTGCTGCTCGGGCCCGTCGCGTACTACATCCGCTCGAAGGTCG
ACGAGACGCCCGAATTCCTCGCGGCCGAAAGCACCGCGACCCCGTTGCGCGACACGTTCGCGTCGCACAAGGCGCGGCTC
GTCGCCGCGATGGGCGTGGTCGTGCTCGGCACCGTCGCGACCTATCTCGTGCTGTTCATGCCGACCTACGGCGTGAAGCA
GCTCGGCCTCGCACCGTCGGCCGCGTTCGCGGCGATCCTCGTCGTCGGCGTGATCCAGATGGCGTTCGCGCCGCTCGTCG
GCCACTGGTCGGATCGCTACGGCCGCGTGCGCGTGATGATCGCGCCGGCGCTCGGCATTCTCGTGCTGATCTATCCGGCA
TTCGCGTACCTAGTCGCGCATCCGGGCTTCGGCACGCTGATCGCGCTGCAGGTGCTGCTCGCGTTCCTGATGACCGGCTA
TTTCGCGGCGTTGCCGGGCCTGTTGTCGGAAGTGTTTCCGGTGCAGACGCGCACGACCGGGATGTCGCTCGCGTATAACG
TCGCGGTGACGATCTTCGGCGGCTTCGGGCCGTTCATCATCGCGTGGCTGATCCGCGCGACCGGGATGAAGACCGCGCCG
AGCTTCTACCTGATGTTCGCGGCCGTGCTGAGCCTCGCGGCGCTGTTCGTGCTGCGGCGGCGGTTCGGGTTCCGGTGA

Upstream 100 bases:

>100_bases
GGGTCGCGGCGGTTTTTTTTGCGCGCCTATAATCGTCGGCACAGCTCGCGGCGCGGGAACGGCGCGTCGGACAGAACGTA
TAACGACAAGGAGATCGCCG

Downstream 100 bases:

>100_bases
AGTCGCGGTGACGCGGGTGGAGGGTCGATCGCCGCTTGCATGAAAAAAGGCCGGTCCAACAGGACCGGCCTTCGTATATC
AGCCGAAACGGCGCGGGCTC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MHAVTQSSSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVLG
AYADRAGRKGALTLSILLMMAGTLVIAVLPTYGTIGVAAPLILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGTVLNAQLTAEQMAAWGWRIPFFFGLLLGPVAYYIRSKVDETPEFLAAESTATPLRDTFASHKARL
VAAMGVVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDRYGRVRVMIAPALGILVLIYPA
FAYLVAHPGFGTLIALQVLLAFLMTGYFAALPGLLSEVFPVQTRTTGMSLAYNVAVTIFGGFGPFIIAWLIRATGMKTAP
SFYLMFAAVLSLAALFVLRRRFGFR

Sequences:

>Translated_425_residues
MHAVTQSSSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVLG
AYADRAGRKGALTLSILLMMAGTLVIAVLPTYGTIGVAAPLILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGTVLNAQLTAEQMAAWGWRIPFFFGLLLGPVAYYIRSKVDETPEFLAAESTATPLRDTFASHKARL
VAAMGVVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDRYGRVRVMIAPALGILVLIYPA
FAYLVAHPGFGTLIALQVLLAFLMTGYFAALPGLLSEVFPVQTRTTGMSLAYNVAVTIFGGFGPFIIAWLIRATGMKTAP
SFYLMFAAVLSLAALFVLRRRFGFR
>Mature_425_residues
MHAVTQSSSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVLG
AYADRAGRKGALTLSILLMMAGTLVIAVLPTYGTIGVAAPLILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGTVLNAQLTAEQMAAWGWRIPFFFGLLLGPVAYYIRSKVDETPEFLAAESTATPLRDTFASHKARL
VAAMGVVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDRYGRVRVMIAPALGILVLIYPA
FAYLVAHPGFGTLIALQVLLAFLMTGYFAALPGLLSEVFPVQTRTTGMSLAYNVAVTIFGGFGPFIIAWLIRATGMKTAP
SFYLMFAAVLSLAALFVLRRRFGFR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=422, Percent_Identity=31.5165876777251, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1788942, Length=438, Percent_Identity=36.7579908675799, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788292, Length=391, Percent_Identity=31.9693094629156, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789941, Length=427, Percent_Identity=34.192037470726, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI87082231, Length=459, Percent_Identity=25.7080610021786, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082404, Length=382, Percent_Identity=24.6073298429319, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45185; Mature: 45185

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCC
>Mature Secondary Structure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HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]