BacMap

Definition	Burkholderia ambifaria AMMD chromosome chromosome 1, complete sequence.
Accession	NC_008390
Length	3,556,545

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The map label for this gene is fadB1 [H]

Identifier: 115350503

GI number: 115350503

Start: 501166

End: 501942

Strand: Direct

Name: fadB1 [H]

Synonym: Bamb_0449

Alternate gene names: 115350503

Gene position: 501166-501942 (Clockwise)

Preceding gene: 115350497

Following gene: 115350504

Centisome position: 14.09

GC content: 64.48

Gene sequence:

>777_bases
ATGTCTTACGAGAACATCCTGGTGGAGACCCGGGGGCGTGTCGGGCTGGTCACGCTCAACCGCCCGAAGGCGCTCAATGC
GCTGAACGATGCGTTGATGGATGAGTTGGGCGCTGCGCTGAAAGCGTTCGACGCGGACGACGAGATCGGCGCGATCGTCG
TGACGGGGAGCGAGAAGGCGTTCGCGGCCGGGGCCGACATCGGCATGATGGCGACCTATTCCTATATGGATGTGTACCGC
GGCGACTACATCACGCGTAACTGGGAGACGGTCCGCGAGATCCGCAAGCCGATCATTGCTGCCGTGGCGGGGTTCGCGCT
CGGCGGCGGCTGCGAGCTCGCGATGATGTGCGACATCATCTTCGCGGCGGATACAGCCAAGTTCGGCCAGCCCGAAATCA
AGCTGGGCGTGATGCCGGGTGCGGGCGGCACGCAGCGCCTGCCGCGCGCGGTGTCGAAGGCGAAGGCGATGGACATGTGC
CTGACCGCGCGCTTCATGGATGCGGCCGAGGCTGAGCGGGCCGGGCTCGTTTCGCGCGTGCTGCCGGCCGACAAGTTGCT
CGACGAAGCGATTGCCGCGGCGACGACGATCGCCGAGTTTTCGCTGCCGGCGGTCATGATGGTCAAGGAGTCGGTGAACC
GTGCGTATGAGACGACGCTGGCCGAGGGCGTTCATTTCGAGCGTCGGCTGTTCCATTCGCTGTTCGCGACCGAAGATCAG
AAGGAAGGGATGGCGGCATTCGTCGAGAAGCGGAAGCCGGTATTCAAGAACCGCTGA

Upstream 100 bases:

>100_bases
TCGGATCGATTAATTAACCGACCGGTTGGTCGGAGAATGGTAGCATCAATCTATTCGCGTGTGCGAGGCGTTTCTCATTT
CATTGATCGAGGAGAAATAG

Downstream 100 bases:

>100_bases
TCCGGTGGCGACGGGTGTCGGTCGCCGGCAGGATCTTCTGGTGCAACGGACCCCAAGCCCCCGTGCGCGGCAGCGCACGA
GGGCTTTTTCAAAATATTTT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALKAFDADDEIGAIVVTGSEKAFAAGADIGMMATYSYMDVYR
GDYITRNWETVREIRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVMPGAGGTQRLPRAVSKAKAMDMC
LTARFMDAAEAERAGLVSRVLPADKLLDEAIAAATTIAEFSLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
KEGMAAFVEKRKPVFKNR

Sequences:

>Translated_258_residues
MSYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALKAFDADDEIGAIVVTGSEKAFAAGADIGMMATYSYMDVYR
GDYITRNWETVREIRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVMPGAGGTQRLPRAVSKAKAMDMC
LTARFMDAAEAERAGLVSRVLPADKLLDEAIAAATTIAEFSLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
KEGMAAFVEKRKPVFKNR
>Mature_257_residues
SYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALKAFDADDEIGAIVVTGSEKAFAAGADIGMMATYSYMDVYRG
DYITRNWETVREIRKPIIAAVAGFALGGGCELAMMCDIIFAADTAKFGQPEIKLGVMPGAGGTQRLPRAVSKAKAMDMCL
TARFMDAAEAERAGLVSRVLPADKLLDEAIAAATTIAEFSLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQK
EGMAAFVEKRKPVFKNR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=260, Percent_Identity=55.7692307692308, Blast_Score=293, Evalue=1e-79,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=36.71875, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI70995211, Length=267, Percent_Identity=33.7078651685393, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI31542718, Length=245, Percent_Identity=29.3877551020408, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI20127408, Length=187, Percent_Identity=35.2941176470588, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI68989263, Length=203, Percent_Identity=34.4827586206897, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=31.5573770491803, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI37594471, Length=212, Percent_Identity=29.7169811320755, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI37594469, Length=212, Percent_Identity=29.7169811320755, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI45643119, Length=246, Percent_Identity=28.0487804878049, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI260275230, Length=246, Percent_Identity=28.0487804878049, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI260274832, Length=246, Percent_Identity=28.0487804878049, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=24.9027237354086, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=24.9027237354086, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI213417737, Length=228, Percent_Identity=25.8771929824561, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=25.8771929824561, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=24.609375, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=24.609375, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI4757966, Length=211, Percent_Identity=27.0142180094787, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI51538581, Length=211, Percent_Identity=27.0142180094787, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221307494, Length=185, Percent_Identity=29.1891891891892, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136756, Length=256, Percent_Identity=24.21875, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136753, Length=256, Percent_Identity=24.21875, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI261878539, Length=108, Percent_Identity=35.1851851851852, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI157694520, Length=206, Percent_Identity=25.2427184466019, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=45.4545454545455, Blast_Score=217, Evalue=5e-58,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=35.7692307692308, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1788597, Length=260, Percent_Identity=33.4615384615385, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787660, Length=269, Percent_Identity=31.9702602230483, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=32.4528301886792, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788682, Length=207, Percent_Identity=31.4009661835749, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1790281, Length=180, Percent_Identity=30, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=55.3784860557769, Blast_Score=291, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=54.320987654321, Blast_Score=263, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=33.8582677165354, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=31.3636363636364, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=32.0675105485232, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=35.7142857142857, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25144276, Length=183, Percent_Identity=36.6120218579235, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=183, Percent_Identity=36.6120218579235, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=183, Percent_Identity=36.6120218579235, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=27.34375, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=274, Percent_Identity=30.6569343065693, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25144157, Length=214, Percent_Identity=30.3738317757009, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=214, Percent_Identity=30.3738317757009, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=179, Percent_Identity=34.6368715083799, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25141363, Length=199, Percent_Identity=28.643216080402, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=24.3243243243243, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=27.319587628866, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129971, Length=259, Percent_Identity=58.3011583011583, Blast_Score=301, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24653477, Length=259, Percent_Identity=58.3011583011583, Blast_Score=301, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=36.8421052631579, Blast_Score=148, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21357171, Length=256, Percent_Identity=32.03125, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=33.3333333333333, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=26.4822134387352, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24654903, Length=191, Percent_Identity=27.2251308900524, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921000, Length=170, Percent_Identity=34.1176470588235, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583077, Length=170, Percent_Identity=34.1176470588235, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583079, Length=170, Percent_Identity=34.1176470588235, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571730, Length=241, Percent_Identity=25.7261410788382, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28571729, Length=241, Percent_Identity=25.7261410788382, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27959; Mature: 27827

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALKAFDADDEIGAIVVTGSEKA
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
FAAGADIGMMATYSYMDVYRGDYITRNWETVREIRKPIIAAVAGFALGGGCELAMMCDII
EECCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGVMPGAGGTQRLPRAVSKAKAMDMCLTARFMDAAEAERAGLVSRV
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPADKLLDEAIAAATTIAEFSLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
KEGMAAFVEKRKPVFKNR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALKAFDADDEIGAIVVTGSEKA
CCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
FAAGADIGMMATYSYMDVYRGDYITRNWETVREIRKPIIAAVAGFALGGGCELAMMCDII
EECCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FAADTAKFGQPEIKLGVMPGAGGTQRLPRAVSKAKAMDMCLTARFMDAAEAERAGLVSRV
HHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPADKLLDEAIAAATTIAEFSLPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
KEGMAAFVEKRKPVFKNR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]