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The map label for this gene is potB

Identifier: 11499199

GI number: 11499199

Start: 1442769

End: 1443515

Strand: Reverse

Name: potB

Synonym: AF1607

Alternate gene names: 11499199

Gene position: 1443515-1442769 (Counterclockwise)

Preceding gene: 11499200

Following gene: 11499198

Centisome position: 66.26

GC content: 50.33

Gene sequence:

>747_bases
ATGAAGAAACTATCCCTCCCCTCAATCATCTTCCTTCTCGCTTTCTTCTACATTCCATTCGCCATCGTCATACTTTACGC
TTTTGGCTATCCCGAATTCACGCTAAAGCACTTCAGAGACGTGCTCAATCCTCTTTACGCCAGCTACATCCTCAACTCAG
CCATCATCGCCTTTCTGACCACAGCAATCTGTCTTGCAATTGCCTACCCCGTTGCGTACTACATAGCCCTTAAGGCCGGA
AAGTGGAAAAATGCCCTGCTCATCGCGGTAATACTGCCCTTCTGGATTAGCTTTCTCCTCAGAACATACGCCCTGATGAC
GATTCTCTCGAAGCTGGGCATAATGTTCACCTTCACCGCGGTGATGGTTGGAATGGTTTACGACTACCTTCCCTTTATGA
TCCTGCCCCTATACACCGCAATGGAAAGGATGAACGTCTCGACAATCGAGGCTTCATACGTGCTCGGCGCAGACCCGATA
AAAACCTTTTTAAAGGTGGTAATGCCTCTCTCAAGGCAGGGGATGATTGCAGGCATTTTTCTCGTTTTCGTTCCGGCCCT
CGGCGAGTTCGTCGTACCAGCGATGCTTGGCGGGGTTTCAGCATCCACGCTCGGAACGCTCACGTGGGAGCTATTCATCA
GATACCACAACTGGTGGAAGGGCTCTGCTCTCTCGGTCATTTACCTGGCTGTTGTGTTTGCCTTCACAGCTTACTATATA
AAGAGGGCGGGGAGGCTGGAGCTATGA

Upstream 100 bases:

>100_bases
ATGTTGTAGTGAAGGCCTTCGCCCCGCCGCAGGAAAATGTTAGGGAAGGGGAAAGGGTGATTGTTGGTTGGGATAGTAGG
GATGCGGTAATCTTGGAGGC

Downstream 100 bases:

>100_bases
TTCTGAGGGCTTTCACCATTGCAGTCTTCGCCTTCCTCTACATCCCCATCGTGATCATGATAGCCCTCTCCTTCTACGAT
GACGGCTTTACGCTGAGGAA

Product: spermidine/putrescine ABC transporter, permease protein (potB)

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKKLSLPSIIFLLAFFYIPFAIVILYAFGYPEFTLKHFRDVLNPLYASYILNSAIIAFLTTAICLAIAYPVAYYIALKAG
KWKNALLIAVILPFWISFLLRTYALMTILSKLGIMFTFTAVMVGMVYDYLPFMILPLYTAMERMNVSTIEASYVLGADPI
KTFLKVVMPLSRQGMIAGIFLVFVPALGEFVVPAMLGGVSASTLGTLTWELFIRYHNWWKGSALSVIYLAVVFAFTAYYI
KRAGRLEL

Sequences:

>Translated_248_residues
MKKLSLPSIIFLLAFFYIPFAIVILYAFGYPEFTLKHFRDVLNPLYASYILNSAIIAFLTTAICLAIAYPVAYYIALKAG
KWKNALLIAVILPFWISFLLRTYALMTILSKLGIMFTFTAVMVGMVYDYLPFMILPLYTAMERMNVSTIEASYVLGADPI
KTFLKVVMPLSRQGMIAGIFLVFVPALGEFVVPAMLGGVSASTLGTLTWELFIRYHNWWKGSALSVIYLAVVFAFTAYYI
KRAGRLEL
>Mature_248_residues
MKKLSLPSIIFLLAFFYIPFAIVILYAFGYPEFTLKHFRDVLNPLYASYILNSAIIAFLTTAICLAIAYPVAYYIALKAG
KWKNALLIAVILPFWISFLLRTYALMTILSKLGIMFTFTAVMVGMVYDYLPFMILPLYTAMERMNVSTIEASYVLGADPI
KTFLKVVMPLSRQGMIAGIFLVFVPALGEFVVPAMLGGVSASTLGTLTWELFIRYHNWWKGSALSVIYLAVVFAFTAYYI
KRAGRLEL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=220, Percent_Identity=39.5454545454545, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1787369, Length=231, Percent_Identity=35.9307359307359, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1787713, Length=196, Percent_Identity=35.2040816326531, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27927; Mature: 27927

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCCC
>Mature Secondary Structure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HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]