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The map label for this gene is braC-4

Identifier: 11498987

GI number: 11498987

Start: 1240776

End: 1242053

Strand: Reverse

Name: braC-4

Synonym: AF1391

Alternate gene names: 11498987

Gene position: 1242053-1240776 (Counterclockwise)

Preceding gene: 11498993

Following gene: 11498986

Centisome position: 57.02

GC content: 50.31

Gene sequence:

>1278_bases
ATGCGAAGGGGGAACAAAATAGTTTTGGCGGCCGTATTAATGGCTGTAGTGCTTGCGCTCTGTGCACAACCAGCAGAGCA
GAAGCCGGGGGAGGGTCAGAAAACAGCAATAAAAATAGGCGTGATGATTGACCTCTCCGGCCCGCTGACAACCTACGGAA
ACAGCATCAAGAACACGCTGGAAATTGCGAAGGAAGATATAAACGCGTATCTTGAGGAGAAGGGTTTGCCCTACACTGTG
GAGTTCTACGTCGAGGACACCAAGGTTGACCCAAAGATAGCTCTTGAAAAGATTCAGAGCCTGCATTCTAAAGGGATTAA
CCTTGTTATAGGGCCAATGGGTAGCGGAGAAGTGAGCAATATAAAGGGGTATGTACAGTCAAACAAAATAATAATAATCT
CACCGTCTTCCACATTGCTGCCCAGCCTCCTCGGAATTACCAAGCCCGAAGATAAGAAGTACATCTTCAGATTTGTTGGT
ACTGACGACCTGCAGACTGACGCAATAACGGCTGAACTCAAGGACCTTGGCATTAAGGCAGTAATCATAACCTACACCGG
AAACGACTGGGGTAAGGGGCTTTATGAGACTATTAAACCCAAGCTCGATGCAGCAGGAATTGAAATCGTTGGCGAACCGG
TAGAATATCCGTCACCACCACCGGCGGACTTCTCCCCGTACATCGCGACGCTCGAAAGCAAGCTGAACGACGCTCTCCAG
AAGTATGACGCTTCAGAAGTTGCCGTTGTAGCTTTCAGCTACGAAGAGGCGGCGACAATGCTCGCTCAGACCAAGGATGA
CAGCCCACTGCTGAACGTCCTGTGGCTGGGCTGCGATGGTACTGCGCTGAGCGATAAAATGCTCGAAGTCTGCGACAAGG
CGAGCAAAGTCAGAATGCTGAGCACGCTCTTCGAATCAAAAGGAGAAGCCTATGAGCAGCTGGCGAAGAAGTACAAGGAG
AGAGGGTTCGGAGATTCGCCCTACCAGTACGCTCTAAACGCCTACGATGCGGCATGGGTGCTGGTTCTCAGCTACATTGA
GGTCGTTAAGGAGAAGGGCAGCTACGATCCAGATGCTATGAACGCCAAGATTCCCGAAGTGACGGAGAAGTACAGCAAGG
GAGAGTACGGAGTAAAGCCGGTCTCGGGTTACATAAAGCTCAACGAGTGGAATGACAGAGCAAGCGGTGACTACGCAATC
TACGAAGTAACTCAGGACTGCAAGTGGCAGACTGCCGGCATTTGGAAGTTCACGGAAAACAAGATAGAGTGGCTATGA

Upstream 100 bases:

>100_bases
GACCCTTCGATAATATTTTTGACGATCTCGAAAGATGAAATAGTATCCATAGACTCCGAGCTTTAGTAAAGTTTTAAAAT
TTTAACGTTGTATAAACGCT

Downstream 100 bases:

>100_bases
AGATTTTACAAACTCAAAATCTTTCCAAATTTTTTGACGGTCTCAAGGCTTTGAACAGAGTCAATATTGGTGTGGAAAAG
GGCAGTATAACGCTCGTCAT

Product: branched-chain amino acid ABC transporter, periplasmic binding protein (braC-4)

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MRRGNKIVLAAVLMAVVLALCAQPAEQKPGEGQKTAIKIGVMIDLSGPLTTYGNSIKNTLEIAKEDINAYLEEKGLPYTV
EFYVEDTKVDPKIALEKIQSLHSKGINLVIGPMGSGEVSNIKGYVQSNKIIIISPSSTLLPSLLGITKPEDKKYIFRFVG
TDDLQTDAITAELKDLGIKAVIITYTGNDWGKGLYETIKPKLDAAGIEIVGEPVEYPSPPPADFSPYIATLESKLNDALQ
KYDASEVAVVAFSYEEAATMLAQTKDDSPLLNVLWLGCDGTALSDKMLEVCDKASKVRMLSTLFESKGEAYEQLAKKYKE
RGFGDSPYQYALNAYDAAWVLVLSYIEVVKEKGSYDPDAMNAKIPEVTEKYSKGEYGVKPVSGYIKLNEWNDRASGDYAI
YEVTQDCKWQTAGIWKFTENKIEWL

Sequences:

>Translated_425_residues
MRRGNKIVLAAVLMAVVLALCAQPAEQKPGEGQKTAIKIGVMIDLSGPLTTYGNSIKNTLEIAKEDINAYLEEKGLPYTV
EFYVEDTKVDPKIALEKIQSLHSKGINLVIGPMGSGEVSNIKGYVQSNKIIIISPSSTLLPSLLGITKPEDKKYIFRFVG
TDDLQTDAITAELKDLGIKAVIITYTGNDWGKGLYETIKPKLDAAGIEIVGEPVEYPSPPPADFSPYIATLESKLNDALQ
KYDASEVAVVAFSYEEAATMLAQTKDDSPLLNVLWLGCDGTALSDKMLEVCDKASKVRMLSTLFESKGEAYEQLAKKYKE
RGFGDSPYQYALNAYDAAWVLVLSYIEVVKEKGSYDPDAMNAKIPEVTEKYSKGEYGVKPVSGYIKLNEWNDRASGDYAI
YEVTQDCKWQTAGIWKFTENKIEWL
>Mature_425_residues
MRRGNKIVLAAVLMAVVLALCAQPAEQKPGEGQKTAIKIGVMIDLSGPLTTYGNSIKNTLEIAKEDINAYLEEKGLPYTV
EFYVEDTKVDPKIALEKIQSLHSKGINLVIGPMGSGEVSNIKGYVQSNKIIIISPSSTLLPSLLGITKPEDKKYIFRFVG
TDDLQTDAITAELKDLGIKAVIITYTGNDWGKGLYETIKPKLDAAGIEIVGEPVEYPSPPPADFSPYIATLESKLNDALQ
KYDASEVAVVAFSYEEAATMLAQTKDDSPLLNVLWLGCDGTALSDKMLEVCDKASKVRMLSTLFESKGEAYEQLAKKYKE
RGFGDSPYQYALNAYDAAWVLVLSYIEVVKEKGSYDPDAMNAKIPEVTEKYSKGEYGVKPVSGYIKLNEWNDRASGDYAI
YEVTQDCKWQTAGIWKFTENKIEWL

Specific function: This Protein Is A Component Of The Leucine-Specific Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids In E.Coli. [C]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 46862; Mature: 46862

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRGNKIVLAAVLMAVVLALCAQPAEQKPGEGQKTAIKIGVMIDLSGPLTTYGNSIKNTL
CCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCHHHCCHHHHHHH
EIAKEDINAYLEEKGLPYTVEFYVEDTKVDPKIALEKIQSLHSKGINLVIGPMGSGEVSN
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHH
IKGYVQSNKIIIISPSSTLLPSLLGITKPEDKKYIFRFVGTDDLQTDAITAELKDLGIKA
HHHEEECCEEEEECCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEE
VIITYTGNDWGKGLYETIKPKLDAAGIEIVGEPVEYPSPPPADFSPYIATLESKLNDALQ
EEEEEECCCHHCCHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KYDASEVAVVAFSYEEAATMLAQTKDDSPLLNVLWLGCDGTALSDKMLEVCDKASKVRML
HCCCCCEEEEEEEHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
STLFESKGEAYEQLAKKYKERGFGDSPYQYALNAYDAAWVLVLSYIEVVKEKGSYDPDAM
HHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NAKIPEVTEKYSKGEYGVKPVSGYIKLNEWNDRASGDYAIYEVTQDCKWQTAGIWKFTEN
CCCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCEEECCEEEEECC
KIEWL
CCCCC
>Mature Secondary Structure
MRRGNKIVLAAVLMAVVLALCAQPAEQKPGEGQKTAIKIGVMIDLSGPLTTYGNSIKNTL
CCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCHHHCCHHHHHHH
EIAKEDINAYLEEKGLPYTVEFYVEDTKVDPKIALEKIQSLHSKGINLVIGPMGSGEVSN
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHH
IKGYVQSNKIIIISPSSTLLPSLLGITKPEDKKYIFRFVGTDDLQTDAITAELKDLGIKA
HHHEEECCEEEEECCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEE
VIITYTGNDWGKGLYETIKPKLDAAGIEIVGEPVEYPSPPPADFSPYIATLESKLNDALQ
EEEEEECCCHHCCHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KYDASEVAVVAFSYEEAATMLAQTKDDSPLLNVLWLGCDGTALSDKMLEVCDKASKVRML
HCCCCCEEEEEEEHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
STLFESKGEAYEQLAKKYKERGFGDSPYQYALNAYDAAWVLVLSYIEVVKEKGSYDPDAM
HHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NAKIPEVTEKYSKGEYGVKPVSGYIKLNEWNDRASGDYAIYEVTQDCKWQTAGIWKFTEN
CCCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCEEECCEEEEECC
KIEWL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]