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The map label for this gene is vhtD-2

Identifier: 11498974

GI number: 11498974

Start: 1230299

End: 1230805

Strand: Reverse

Name: vhtD-2

Synonym: AF1378

Alternate gene names: 11498974

Gene position: 1230805-1230299 (Counterclockwise)

Preceding gene: 11498975

Following gene: 11498973

Centisome position: 56.5

GC content: 49.51

Gene sequence:

>507_bases
TTGTCTCGGATTCTGGTCGTGGGAGTAGGGAACCCGCTGATGGGTGATGATGGCCTCGGCATCAGAGTTGTGGAGGAGCT
CAAGCGAAGAAGGCTGCCAAAAAACGTCACCGTGATGGATGGTGGAACGGGAAGCTTTGATCTCGTGAATTACTTCTTTT
TTTACGACGTCGTTGTGATAGTTGATGCGGTCAATTTCGGCGGAAAGCCGGGGAGAGTTTACAGGTTACCGGTCGAGAGA
GTGCTGAAAAGGAAAATTGCGTCAATCCACGACCTCGACATTTTCTACGCCATATCGGCGATGAGTGCGATTCAGGCAAT
GCCCAGAGTGATTCTCATCGGTGCAGAGGTGAAAAACCTTGCAGAGTTTTCTGAGATGTCCTCTGAAGCTGAGGAGGCCG
TAAAAAAAGTGGTAAAAAGAATTGTTAAACTGGTCACTCCCTCATCAGGTAGAAGAGCGAGGATCCGAAGAACGCGAGTC
CAACGACTAAAGCCAGCTCCCACATAA

Upstream 100 bases:

>100_bases
ACGATGTTTCTCTATGCAACAGTGATGATAATGCACGCCTACGCAGCAACGATATTCGGAACGCTGTCATCAATGATAAG
CGGCTACAGGAGGGAAAAAG

Downstream 100 bases:

>100_bases
AAGTTAGTTAACATATTAATTTATTAACTTTTTTCAACTAAAAATTGCACAATTTTTCATGCATCTATTAACTTTTACAA
TTTTTCATGTATGTAATTAT

Product: F420-nonreducing hydrogenase (vhtD-2)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MSRILVVGVGNPLMGDDGLGIRVVEELKRRRLPKNVTVMDGGTGSFDLVNYFFFYDVVVIVDAVNFGGKPGRVYRLPVER
VLKRKIASIHDLDIFYAISAMSAIQAMPRVILIGAEVKNLAEFSEMSSEAEEAVKKVVKRIVKLVTPSSGRRARIRRTRV
QRLKPAPT

Sequences:

>Translated_168_residues
MSRILVVGVGNPLMGDDGLGIRVVEELKRRRLPKNVTVMDGGTGSFDLVNYFFFYDVVVIVDAVNFGGKPGRVYRLPVER
VLKRKIASIHDLDIFYAISAMSAIQAMPRVILIGAEVKNLAEFSEMSSEAEEAVKKVVKRIVKLVTPSSGRRARIRRTRV
QRLKPAPT
>Mature_167_residues
SRILVVGVGNPLMGDDGLGIRVVEELKRRRLPKNVTVMDGGTGSFDLVNYFFFYDVVVIVDAVNFGGKPGRVYRLPVERV
LKRKIASIHDLDIFYAISAMSAIQAMPRVILIGAEVKNLAEFSEMSSEAEEAVKKVVKRIVKLVTPSSGRRARIRRTRVQ
RLKPAPT

Specific function: Not known. Could be involved in the processing of hydrogenase [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=134, Percent_Identity=31.3432835820896, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI1789367, Length=146, Percent_Identity=31.5068493150685, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 18712; Mature: 18581

Theoretical pI: Translated: 11.02; Mature: 11.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRILVVGVGNPLMGDDGLGIRVVEELKRRRLPKNVTVMDGGTGSFDLVNYFFFYDVVVI
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
VDAVNFGGKPGRVYRLPVERVLKRKIASIHDLDIFYAISAMSAIQAMPRVILIGAEVKNL
HHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHH
AEFSEMSSEAEEAVKKVVKRIVKLVTPSSGRRARIRRTRVQRLKPAPT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRILVVGVGNPLMGDDGLGIRVVEELKRRRLPKNVTVMDGGTGSFDLVNYFFFYDVVVI
CCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
VDAVNFGGKPGRVYRLPVERVLKRKIASIHDLDIFYAISAMSAIQAMPRVILIGAEVKNL
HHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHH
AEFSEMSSEAEEAVKKVVKRIVKLVTPSSGRRARIRRTRVQRLKPAPT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8230232; 8163174; 12597275 [H]