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The map label for this gene is tpiA

Identifier: 11498902

GI number: 11498902

Start: 1163419

End: 1164090

Strand: Reverse

Name: tpiA

Synonym: AF1304

Alternate gene names: 11498902

Gene position: 1164090-1163419 (Counterclockwise)

Preceding gene: 11498903

Following gene: 11498896

Centisome position: 53.44

GC content: 54.02

Gene sequence:

>672_bases
GTGAGAAAAATGGGGGTTGTGATAATAAACTTCAAGGCGTATAAGGAGGGATTTGGAAAAAGGGCGCTTGAGCTGGCGAA
GATTGCCGAAAACGTGGCGTCGAGATGCGATGAGTATGTTGGCATAGCCGTCTCCTTCCTCGACCTCCCCGTAATCGCAA
GGGAGGTCAGCATCGACGTTTACGCGCAGCATGTTGATGCCGTTGGCTTCGGGAGCCACACAGGCAGAATAAACGCCGAT
ATGATCGCGGAATACGGGGCAAAGGGAAGCTTGGTCAATCATTCTGAGAGAAGGCTGAAGCTCGCCGATATTGAGTTCAA
CGTGAGCAGGCTGAGGGAGCTTGGTCTCACTTCGGTTGTCTGCACGAACAACGTCCCAACAACAGCCGCGGCGGCAGCGT
TGAATCCGGACTTTGTCGCGGTGGAGCCACCTGAGCTGATAGGTAGCGGAATTCCGGTAAGCAAGGCCGAACCCGAGGTT
GTGGAGAACTCAGTTAAGGCTGCAAAGGAAGTCAACAAGAGCGTTAGAGTTCTTTGTGGGGCGGGCATAACCACCCATGA
GGACTACGTAAAAGCATTGGAGCTCGGAGCAGAGGGTGTGCTACTCGCGAGTGGGGTTGTTAAGGCCGAAGACCAGAAGA
GGGCACTTGAGGAGCTCGTAGGGCTTCTTTAG

Upstream 100 bases:

>100_bases
GCTGTAACGAAGGCCAGAGACGTTGAGGGAGCTGTAAGGAAATTCATGAGCCACATGAAGCCCGACACAGACCAGTTCCG
CATTATGACGGACTTTTGAG

Downstream 100 bases:

>100_bases
AAGTCCCTGTAAAATTCCTTTTTAGCCTTTTTGAAGTTTCCTCTGTTCCTCCTTCTCGGAGGCTCGTTCAAAAGCTCCTC
AAGCTCCTCATCCGTAACAG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MRKMGVVIINFKAYKEGFGKRALELAKIAENVASRCDEYVGIAVSFLDLPVIAREVSIDVYAQHVDAVGFGSHTGRINAD
MIAEYGAKGSLVNHSERRLKLADIEFNVSRLRELGLTSVVCTNNVPTTAAAAALNPDFVAVEPPELIGSGIPVSKAEPEV
VENSVKAAKEVNKSVRVLCGAGITTHEDYVKALELGAEGVLLASGVVKAEDQKRALEELVGLL

Sequences:

>Translated_223_residues
MRKMGVVIINFKAYKEGFGKRALELAKIAENVASRCDEYVGIAVSFLDLPVIAREVSIDVYAQHVDAVGFGSHTGRINAD
MIAEYGAKGSLVNHSERRLKLADIEFNVSRLRELGLTSVVCTNNVPTTAAAAALNPDFVAVEPPELIGSGIPVSKAEPEV
VENSVKAAKEVNKSVRVLCGAGITTHEDYVKALELGAEGVLLASGVVKAEDQKRALEELVGLL
>Mature_223_residues
MRKMGVVIINFKAYKEGFGKRALELAKIAENVASRCDEYVGIAVSFLDLPVIAREVSIDVYAQHVDAVGFGSHTGRINAD
MIAEYGAKGSLVNHSERRLKLADIEFNVSRLRELGLTSVVCTNNVPTTAAAAALNPDFVAVEPPELIGSGIPVSKAEPEV
VENSVKAAKEVNKSVRVLCGAGITTHEDYVKALELGAEGVLLASGVVKAEDQKRALEELVGLL

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

None

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_ARCFU (O28965)

Other databases:

- EMBL:   AE000782
- PIR:   G69412
- RefSeq:   NP_070133.1
- ProteinModelPortal:   O28965
- SMR:   O28965
- GeneID:   1484530
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1304
- NMPDR:   fig|224325.1.peg.1294
- TIGR:   AF_1304
- HOGENOM:   HBG297256
- OMA:   VEPPELI
- ProtClustDB:   PRK04302
- BioCyc:   AFUL224325:AF_1304-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_A
- InterPro:   IPR013785
- InterPro:   IPR000652
- InterPro:   IPR022891
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 23772; Mature: 23772

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 94-94 ACT_SITE 142-142 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKMGVVIINFKAYKEGFGKRALELAKIAENVASRCDEYVGIAVSFLDLPVIAREVSIDV
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAQHVDAVGFGSHTGRINADMIAEYGAKGSLVNHSERRLKLADIEFNVSRLRELGLTSVV
HHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEEEEECHHHHHHCCCCEEE
CTNNVPTTAAAAALNPDFVAVEPPELIGSGIPVSKAEPEVVENSVKAAKEVNKSVRVLCG
EECCCCCHHHHHHCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEC
AGITTHEDYVKALELGAEGVLLASGVVKAEDQKRALEELVGLL
CCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKMGVVIINFKAYKEGFGKRALELAKIAENVASRCDEYVGIAVSFLDLPVIAREVSIDV
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAQHVDAVGFGSHTGRINADMIAEYGAKGSLVNHSERRLKLADIEFNVSRLRELGLTSVV
HHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEEEEECHHHHHHCCCCEEE
CTNNVPTTAAAAALNPDFVAVEPPELIGSGIPVSKAEPEVVENSVKAAKEVNKSVRVLCG
EECCCCCHHHHHHCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEC
AGITTHEDYVKALELGAEGVLLASGVVKAEDQKRALEELVGLL
CCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475