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The map label for this gene is argB

Identifier: 11498879

GI number: 11498879

Start: 1141715

End: 1142587

Strand: Reverse

Name: argB

Synonym: AF1280

Alternate gene names: 11498879

Gene position: 1142587-1141715 (Counterclockwise)

Preceding gene: 11498880

Following gene: 11498878

Centisome position: 52.45

GC content: 49.03

Gene sequence:

>873_bases
ATGGAAAACGTGGAACTGCTGATCGAGGCTCTTCCATATATAAAGGATTTTCACTCCACCACGATGGTTATCAAGATTGG
CGGTCATGCGATGGTCAACGACAGGATTCTTGAGGACACCATCAAGGATATAGTGCTTCTTTACTTCGTCGGTATAAAGC
CGGTGGTGGTGCACGGCGGTGGGCCCGAGATTTCCGAAAAAATGGAGAAATTCGGACTGAAGCCCAAATTTGTTGAAGGA
CTGAGGGTGACTGATAAAGAGACAATGGAAGTTGTGGAGATGGTTCTGGACGGAAAGGTCAACTCTAAGATTGTTACGAC
CTTTATAAGAAACGGCGGCAAGGCCGTTGGCCTTAGCGGGAAGGACGGTTTGCTGATTGTGGCCAGAAAAAAGGAAATGA
GGATGAAGAAGGGTGAAGAGGAGGTAATAATCGACCTCGGCTTTGTTGGGGAGACCGAATTTGTAAATCCCGAAATTATC
AGAATTCTGCTTGATAACGGGTTCATTCCAGTCGTGTCCCCCGTGGCAACCGACCTTGCGGGAAACACCTACAACCTGAA
TGCCGATGTTGTTGCGGGAGACATTGCTGCAGCTTTGAAGGCGAAGAAGCTCATCATGCTAACCGACGTTCCGGGAATAC
TCGAGAATCCGGATGACAAATCAACGCTGATTTCCAGAATCCGGCTGTCAGAGCTCGAGAACATGAGAAGCAAGGGAGTC
ATAAGGGGAGGCATGATACCCAAGGTCGATGCCGTCATTAAGGCTCTGAAAAGCGGAGTTGAGAGGGCCCACATAATCGA
CGGCTCACGTCCCCATTCCATACTGATAGAGCTTTTCACTAAAGAGGGAATCGGGACGATGGTGGAACCGTGA

Upstream 100 bases:

>100_bases
AGCCATCCACGCCGATTTTGGGAATCCAGGCAGTTTTTAACACTTGCCTCCAGCAATGCGGTCTGATAATCCTTAAGTAT
CCCAACCTGAATCTGGACAC

Downstream 100 bases:

>100_bases
TATCGGCGAAGCTCGGAATGCAGCCCGATGTGAGGCACTCTCCGAAAGAGGCCTTTGAGTTCGCCGCAAACAACGGTTTC
ACCCATGTCGAGATTCTGAT

Product: acetylglutamate kinase (argB)

Products: NA

Alternate protein names: N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; AGK

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MENVELLIEALPYIKDFHSTTMVIKIGGHAMVNDRILEDTIKDIVLLYFVGIKPVVVHGGGPEISEKMEKFGLKPKFVEG
LRVTDKETMEVVEMVLDGKVNSKIVTTFIRNGGKAVGLSGKDGLLIVARKKEMRMKKGEEEVIIDLGFVGETEFVNPEII
RILLDNGFIPVVSPVATDLAGNTYNLNADVVAGDIAAALKAKKLIMLTDVPGILENPDDKSTLISRIRLSELENMRSKGV
IRGGMIPKVDAVIKALKSGVERAHIIDGSRPHSILIELFTKEGIGTMVEP

Sequences:

>Translated_290_residues
MENVELLIEALPYIKDFHSTTMVIKIGGHAMVNDRILEDTIKDIVLLYFVGIKPVVVHGGGPEISEKMEKFGLKPKFVEG
LRVTDKETMEVVEMVLDGKVNSKIVTTFIRNGGKAVGLSGKDGLLIVARKKEMRMKKGEEEVIIDLGFVGETEFVNPEII
RILLDNGFIPVVSPVATDLAGNTYNLNADVVAGDIAAALKAKKLIMLTDVPGILENPDDKSTLISRIRLSELENMRSKGV
IRGGMIPKVDAVIKALKSGVERAHIIDGSRPHSILIELFTKEGIGTMVEP
>Mature_290_residues
MENVELLIEALPYIKDFHSTTMVIKIGGHAMVNDRILEDTIKDIVLLYFVGIKPVVVHGGGPEISEKMEKFGLKPKFVEG
LRVTDKETMEVVEMVLDGKVNSKIVTTFIRNGGKAVGLSGKDGLLIVARKKEMRMKKGEEEVIIDLGFVGETEFVNPEII
RILLDNGFIPVVSPVATDLAGNTYNLNADVVAGDIAAALKAKKLIMLTDVPGILENPDDKSTLISRIRLSELENMRSKGV
IRGGMIPKVDAVIKALKSGVERAHIIDGSRPHSILIELFTKEGIGTMVEP

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetylglutamate kinase family

Homologues:

Organism=Escherichia coli, GI1789181, Length=291, Percent_Identity=27.8350515463918, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI145693208, Length=268, Percent_Identity=31.7164179104478, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320913, Length=293, Percent_Identity=28.3276450511945, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ARGB_ARCFU (O28988)

Other databases:

- EMBL:   AE000782
- PIR:   G69409
- RefSeq:   NP_070108.1
- ProteinModelPortal:   O28988
- SMR:   O28988
- GeneID:   1484504
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1280
- NMPDR:   fig|224325.1.peg.1268
- TIGR:   AF_1280
- HOGENOM:   HBG497643
- OMA:   MDIVEMV
- ProtClustDB:   CLSK2746833
- BioCyc:   AFUL224325:AF_1280-MONOMER
- BRENDA:   2.7.2.8
- GO:   GO:0005737
- HAMAP:   MF_00082_A
- InterPro:   IPR004662
- InterPro:   IPR001048
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000728
- TIGRFAMs:   TIGR00761

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.8

Molecular weight: Translated: 31665; Mature: 31665

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: BINDING 82-82 BINDING 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENVELLIEALPYIKDFHSTTMVIKIGGHAMVNDRILEDTIKDIVLLYFVGIKPVVVHGG
CCHHHHHHHHHHHHHHHCCEEEEEEECCCEEECHHHHHHHHHHHHHHHHHCCCEEEEECC
GPEISEKMEKFGLKPKFVEGLRVTDKETMEVVEMVLDGKVNSKIVTTFIRNGGKAVGLSG
CCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCC
KDGLLIVARKKEMRMKKGEEEVIIDLGFVGETEFVNPEIIRILLDNGFIPVVSPVATDLA
CCCEEEEEECHHHHHHCCCCEEEEEEECCCCCCCCCHHHEEEEECCCCCCHHHHHHHHHC
GNTYNLNADVVAGDIAAALKAKKLIMLTDVPGILENPDDKSTLISRIRLSELENMRSKGV
CCEEECCCCEEHHHHHHHHHHCEEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
IRGGMIPKVDAVIKALKSGVERAHIIDGSRPHSILIELFTKEGIGTMVEP
CCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHEEHHCCCCCCCCCC
>Mature Secondary Structure
MENVELLIEALPYIKDFHSTTMVIKIGGHAMVNDRILEDTIKDIVLLYFVGIKPVVVHGG
CCHHHHHHHHHHHHHHHCCEEEEEEECCCEEECHHHHHHHHHHHHHHHHHCCCEEEEECC
GPEISEKMEKFGLKPKFVEGLRVTDKETMEVVEMVLDGKVNSKIVTTFIRNGGKAVGLSG
CCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCC
KDGLLIVARKKEMRMKKGEEEVIIDLGFVGETEFVNPEIIRILLDNGFIPVVSPVATDLA
CCCEEEEEECHHHHHHCCCCEEEEEEECCCCCCCCCHHHEEEEECCCCCCHHHHHHHHHC
GNTYNLNADVVAGDIAAALKAKKLIMLTDVPGILENPDDKSTLISRIRLSELENMRSKGV
CCEEECCCCEEHHHHHHHHHHCEEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
IRGGMIPKVDAVIKALKSGVERAHIIDGSRPHSILIELFTKEGIGTMVEP
CCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHEEHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475