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The map label for this gene is cheB

Identifier: 11498646

GI number: 11498646

Start: 948206

End: 949255

Strand: Reverse

Name: cheB

Synonym: AF1041

Alternate gene names: 11498646

Gene position: 949255-948206 (Counterclockwise)

Preceding gene: 11498647

Following gene: 11498645

Centisome position: 43.58

GC content: 52.95

Gene sequence:

>1050_bases
ATGAGGGTGCTTGTTGTTGACGACTCCGCCCTCGTCAGGATGGCCGTGACCGACATACTTACCAAGGCAGGAATAGAGGT
AGTTGACACGGCGAAGAATGGTAGGGAGGCTGTTGAAAAGGCCCTCAAGCTCAAGCCAGATGTGATTACCCTCGACATCA
ACATGCCAGAAATGGATGGGCTGACAGCGCTGAAGCTGATAATGGAAAAACAGCCGACTCCGGTCGTGATGCTCAGCTCC
CTCACCAAGGAGGGGGCGAGAGAGACTCTGGAGGCTTTAAAGATAGGTGCAGTTGACTTTGTAACAAAACCTGATGGGGC
ACTCGTCGATCTCTCCTCTGTAGCCCAGGAACTAGTCAGGAAGGTGCAGATGGCTGCCTCAACATCGCTGAACGTCCTCA
GACTCCAAAATCTCAAGAAAATTAAGGGGGAGGTTGTTAGAGGTAACTGGAAGGGAAAAACAAAGGACGTGTGCGTCTTA
ATTGGTTCCTCTACAGGTGGACCGTCAGCGCTGGAGATGATAATCCCGAGACTTCCTGCTGACATCCCCGCCCCAGTTTT
TGTGGTGCAGCACATGCCTCCCGGCTTCACAAAGCAGCTGGCGGAGAGGCTCAACTCCATCTCGGAGGTGGAAGTGAAGG
AGGCTGAGAACAACGAGCGCGTTAAGGATGGAGTGGTTTACGTTGCCCCTGGCGGCTACCACATGAAGGTCAGGAGGGCA
GCGAATGTTGTCAGAATAAAGGTGGTTGACGGAGAGCCTGTGAATGCAGTAAAGCCGTCTGTTGATGTCACGGCTGACTC
GGTAGTGCAGGCTTACGGAGGAAACGTTGTTGGGGCCATTCTGACTGGAATGGGTGAAGATGGGGCTTACGGGATGAAGC
TGATAAAGGACAGGGGAGGGTTAACGATAGCCAGCAGCGAGGATACGTGCATGGTCTTCGGCATGCCGAAGGCGGCAATT
GAGCTTGGCGGAATAACTTCGGTAAAGCCCGTTTTTGAGATTGCCGAGGAGATAGTCCGGTTTTTGGAGGTGAAGCTTAA
TGAGCGATGA

Upstream 100 bases:

>100_bases
AGGCAGCGATAAAACTTGGGGCGAAGGGATACATCGTCAAGCCCTTCCAGGCTCCCAAGGTGATTGAGGAGCTGAAGAAG
GTTGCCGGAATGGGCTGAGC

Downstream 100 bases:

>100_bases
AATGGAGGAGTACAAGCAGGAGTTCATACAGGAGGCCAGAGAGTACCTTGACGTGATGAATCAGAACTTCATAAAGGTTG
AGAAAGGGGACGTTGATGCA

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MRVLVVDDSALVRMAVTDILTKAGIEVVDTAKNGREAVEKALKLKPDVITLDINMPEMDGLTALKLIMEKQPTPVVMLSS
LTKEGARETLEALKIGAVDFVTKPDGALVDLSSVAQELVRKVQMAASTSLNVLRLQNLKKIKGEVVRGNWKGKTKDVCVL
IGSSTGGPSALEMIIPRLPADIPAPVFVVQHMPPGFTKQLAERLNSISEVEVKEAENNERVKDGVVYVAPGGYHMKVRRA
ANVVRIKVVDGEPVNAVKPSVDVTADSVVQAYGGNVVGAILTGMGEDGAYGMKLIKDRGGLTIASSEDTCMVFGMPKAAI
ELGGITSVKPVFEIAEEIVRFLEVKLNER

Sequences:

>Translated_349_residues
MRVLVVDDSALVRMAVTDILTKAGIEVVDTAKNGREAVEKALKLKPDVITLDINMPEMDGLTALKLIMEKQPTPVVMLSS
LTKEGARETLEALKIGAVDFVTKPDGALVDLSSVAQELVRKVQMAASTSLNVLRLQNLKKIKGEVVRGNWKGKTKDVCVL
IGSSTGGPSALEMIIPRLPADIPAPVFVVQHMPPGFTKQLAERLNSISEVEVKEAENNERVKDGVVYVAPGGYHMKVRRA
ANVVRIKVVDGEPVNAVKPSVDVTADSVVQAYGGNVVGAILTGMGEDGAYGMKLIKDRGGLTIASSEDTCMVFGMPKAAI
ELGGITSVKPVFEIAEEIVRFLEVKLNER
>Mature_349_residues
MRVLVVDDSALVRMAVTDILTKAGIEVVDTAKNGREAVEKALKLKPDVITLDINMPEMDGLTALKLIMEKQPTPVVMLSS
LTKEGARETLEALKIGAVDFVTKPDGALVDLSSVAQELVRKVQMAASTSLNVLRLQNLKKIKGEVVRGNWKGKTKDVCVL
IGSSTGGPSALEMIIPRLPADIPAPVFVVQHMPPGFTKQLAERLNSISEVEVKEAENNERVKDGVVYVAPGGYHMKVRRA
ANVVRIKVVDGEPVNAVKPSVDVTADSVVQAYGGNVVGAILTGMGEDGAYGMKLIKDRGGLTIASSEDTCMVFGMPKAAI
ELGGITSVKPVFEIAEEIVRFLEVKLNER

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=342, Percent_Identity=42.6900584795322, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1788550, Length=138, Percent_Identity=34.7826086956522, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789402, Length=132, Percent_Identity=31.8181818181818, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_ARCFU (O29221)

Other databases:

- EMBL:   AE000782
- PIR:   A69380
- RefSeq:   NP_069874.1
- ProteinModelPortal:   O29221
- SMR:   O29221
- GeneID:   1484264
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF1041
- NMPDR:   fig|224325.1.peg.1033
- TIGR:   AF_1041
- HOGENOM:   HBG705324
- OMA:   IGRDGAN
- ProtClustDB:   PRK00742
- BioCyc:   AFUL224325:AF_1041-MONOMER
- BRENDA:   3.1.1.61
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 37393; Mature: 37393

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 164-164 ACT_SITE 191-191 ACT_SITE 287-287

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVVDDSALVRMAVTDILTKAGIEVVDTAKNGREAVEKALKLKPDVITLDINMPEMDG
CEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCC
LTALKLIMEKQPTPVVMLSSLTKEGARETLEALKIGAVDFVTKPDGALVDLSSVAQELVR
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHHHHHH
KVQMAASTSLNVLRLQNLKKIKGEVVRGNWKGKTKDVCVLIGSSTGGPSALEMIIPRLPA
HHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCC
DIPAPVFVVQHMPPGFTKQLAERLNSISEVEVKEAENNERVKDGVVYVAPGGYHMKVRRA
CCCCCEEEEECCCCCHHHHHHHHHCCHHHEEEHHCCCCCCHHCCEEEECCCCEEEEEECC
ANVVRIKVVDGEPVNAVKPSVDVTADSVVQAYGGNVVGAILTGMGEDGAYGMKLIKDRGG
CCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHCCCCCCCCCHHEEECCCC
LTIASSEDTCMVFGMPKAAIELGGITSVKPVFEIAEEIVRFLEVKLNER
EEEECCCCEEEEEECCHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVLVVDDSALVRMAVTDILTKAGIEVVDTAKNGREAVEKALKLKPDVITLDINMPEMDG
CEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCC
LTALKLIMEKQPTPVVMLSSLTKEGARETLEALKIGAVDFVTKPDGALVDLSSVAQELVR
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHHHHHH
KVQMAASTSLNVLRLQNLKKIKGEVVRGNWKGKTKDVCVLIGSSTGGPSALEMIIPRLPA
HHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCC
DIPAPVFVVQHMPPGFTKQLAERLNSISEVEVKEAENNERVKDGVVYVAPGGYHMKVRRA
CCCCCEEEEECCCCCHHHHHHHHHCCHHHEEEHHCCCCCCHHCCEEEECCCCEEEEEECC
ANVVRIKVVDGEPVNAVKPSVDVTADSVVQAYGGNVVGAILTGMGEDGAYGMKLIKDRGG
CCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHCCCCCCCCCHHEEECCCC
LTIASSEDTCMVFGMPKAAIELGGITSVKPVFEIAEEIVRFLEVKLNER
EEEECCCCEEEEEECCHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475