Click here to switch to the map view.

The map label for this gene is trzA-2

Identifier: 11498602

GI number: 11498602

Start: 899964

End: 901214

Strand: Reverse

Name: trzA-2

Synonym: AF0997

Alternate gene names: 11498602

Gene position: 901214-899964 (Counterclockwise)

Preceding gene: 11498604

Following gene: 11498601

Centisome position: 41.37

GC content: 53.72

Gene sequence:

>1251_bases
ATGCACGATCTTGTCATCAGAAACGGCCTTTGTTTCATTAACGGGGAGTTTGTCGAGTGCAGCGTTGGTGTGGACGGCAA
CAGAATCACCCATGTAGCCAAGGAGGTTGAGAGGGGAGAAATAGAGATTGACGCTGCTGGCTGCCTCGTCATGCCCGGCT
GCTTCAACGCTCACACTCATGCTGCCATGACCTTGCTGAGGGGTTATGCCGAGGGCTTGCCGCTCAGGGAGTGGCTTGAG
AAGGTCTGGGAGGTTGAGGCAAGGCTGGATGAGGATGCGGTTTACTGGGGAACCATGCTCGCGTGCGTTGAAATGCTGAA
ATCGGGAGTAACCGCATTTGCGGACATGTACATACACATGGATGCAGTCGCTGAGGCTGTGGGCGAGTCGGGAATGAGGG
CAGTTCTCGGCTACGGCATGGCTGACAGAGGTGATGAGGAGAGAGCAAGGAAGGAGCTTGAAATCGGACTTGAATTTGCG
GAGAAGTGGAACGGCGGCTTTGAGGGAAGAGTTACCACCATGCTGGCTCCTCACGCCCCCTACACATGCTCCCCGGAGTT
TTTGAAAGTGGTCAGCGACGCTTCGAAGGATAAGGGATTTTTGAAGCACATACACGTCTCTGAAACTCTGTGGGAGGTAA
AGGAGGTTAGGGAGAGGTACGGAAAAAGGCCCGTTGAGTTCCTGGACTCAATAGGCTTTCTCGACTCCAGCACCGTTCTG
GCTCACGCGGTTTGGCTGTCTGAAGCTGAGATGAAAATTCTTGCAGAGAGGGGCGTTAGCGTCGCACACTGTCCCACAAG
CAACCTGAAGCTCTCGTCAGGCATAGCAAAAGTTTCTGAGCTTCTTGAGATGGGTGTAAACGTGGGCATAGGCACGGATG
GCGCTGCGAGCAACAACATGCTCAGTGTGCTTTCAGACGCGAGAGTGGGTGCTTTGCTTCAGAACCTGAGAGGCAGGACT
CTGAAACCTGGGCACTGGCTTGAGATGGCCACCGAAGGCGGATACAGGGCCTACAACCTTAAGGGAGGGAGAATCGAGGA
GGGATACCTTGCCGACATTGTTGTTTTCAGCAAAACGTGCAGGAACGCTCCGATGCACGATCCAGCAGCGATGCTTTACG
TCGAAAATCAGGCGCTTCATGCTGTTGTTGATGGTGTTCTCGTCATGGAGGACGGCATTCTTGTGAACGTTGAGGAGGAA
AAAGTAATCGAAAAGGCTGAGGAAACGGCACTCGAGCTGGTAGGTGGTTAG

Upstream 100 bases:

>100_bases
GTGTCCTTTCTGCTGAGGAGAACATTCATAAAATATGTTTATTTTCAGCATTTTAAGCTTTACCGCGAAAGGTATTAATC
TTAAAGATTGAGGACCTTAC

Downstream 100 bases:

>100_bases
CCAGAATGCTTGCAAAAATAGGAGAAAATTTTAATTATTTCTTATGTTAACATCATAACAATGCCAAAAATCAATTTCAG
GCAACTGAAATTCAGGGGAA

Product: N-ethylammeline chlorohydrolase (trzA-2)

Products: NA

Alternate protein names: MTA/SAH deaminase 2

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MHDLVIRNGLCFINGEFVECSVGVDGNRITHVAKEVERGEIEIDAAGCLVMPGCFNAHTHAAMTLLRGYAEGLPLREWLE
KVWEVEARLDEDAVYWGTMLACVEMLKSGVTAFADMYIHMDAVAEAVGESGMRAVLGYGMADRGDEERARKELEIGLEFA
EKWNGGFEGRVTTMLAPHAPYTCSPEFLKVVSDASKDKGFLKHIHVSETLWEVKEVRERYGKRPVEFLDSIGFLDSSTVL
AHAVWLSEAEMKILAERGVSVAHCPTSNLKLSSGIAKVSELLEMGVNVGIGTDGAASNNMLSVLSDARVGALLQNLRGRT
LKPGHWLEMATEGGYRAYNLKGGRIEEGYLADIVVFSKTCRNAPMHDPAAMLYVENQALHAVVDGVLVMEDGILVNVEEE
KVIEKAEETALELVGG

Sequences:

>Translated_416_residues
MHDLVIRNGLCFINGEFVECSVGVDGNRITHVAKEVERGEIEIDAAGCLVMPGCFNAHTHAAMTLLRGYAEGLPLREWLE
KVWEVEARLDEDAVYWGTMLACVEMLKSGVTAFADMYIHMDAVAEAVGESGMRAVLGYGMADRGDEERARKELEIGLEFA
EKWNGGFEGRVTTMLAPHAPYTCSPEFLKVVSDASKDKGFLKHIHVSETLWEVKEVRERYGKRPVEFLDSIGFLDSSTVL
AHAVWLSEAEMKILAERGVSVAHCPTSNLKLSSGIAKVSELLEMGVNVGIGTDGAASNNMLSVLSDARVGALLQNLRGRT
LKPGHWLEMATEGGYRAYNLKGGRIEEGYLADIVVFSKTCRNAPMHDPAAMLYVENQALHAVVDGVLVMEDGILVNVEEE
KVIEKAEETALELVGG
>Mature_416_residues
MHDLVIRNGLCFINGEFVECSVGVDGNRITHVAKEVERGEIEIDAAGCLVMPGCFNAHTHAAMTLLRGYAEGLPLREWLE
KVWEVEARLDEDAVYWGTMLACVEMLKSGVTAFADMYIHMDAVAEAVGESGMRAVLGYGMADRGDEERARKELEIGLEFA
EKWNGGFEGRVTTMLAPHAPYTCSPEFLKVVSDASKDKGFLKHIHVSETLWEVKEVRERYGKRPVEFLDSIGFLDSSTVL
AHAVWLSEAEMKILAERGVSVAHCPTSNLKLSSGIAKVSELLEMGVNVGIGTDGAASNNMLSVLSDARVGALLQNLRGRT
LKPGHWLEMATEGGYRAYNLKGGRIEEGYLADIVVFSKTCRNAPMHDPAAMLYVENQALHAVVDGVLVMEDGILVNVEEE
KVIEKAEETALELVGG

Specific function: Catalyzes the deamination of 5-methylthioadenosine and S-adenosyl-L-homocysteine into 5-methylthioinosine and S-inosyl-L- homocysteine, respectively. Is also able to deaminate adenosine

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MTA/SAH deaminase family

Homologues:

Organism=Homo sapiens, GI4758426, Length=342, Percent_Identity=29.5321637426901, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI87082177, Length=395, Percent_Identity=25.8227848101266, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789249, Length=363, Percent_Identity=30.3030303030303, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17540282, Length=342, Percent_Identity=23.9766081871345, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319963, Length=276, Percent_Identity=26.4492753623188, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24643849, Length=352, Percent_Identity=29.8295454545455, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTAD2_ARCFU (O29265)

Other databases:

- EMBL:   AE000782
- PIR:   E69374
- RefSeq:   NP_069830.1
- ProteinModelPortal:   O29265
- SMR:   O29265
- GeneID:   1484220
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0997
- NMPDR:   fig|224325.1.peg.989
- TIGR:   AF_0997
- HOGENOM:   HBG668170
- OMA:   KVIDMAT
- ProtClustDB:   CLSK2746704
- BioCyc:   AFUL224325:AF_0997-MONOMER
- BRENDA:   3.5.4.28
- HAMAP:   MF_01281
- InterPro:   IPR006680
- InterPro:   IPR011059

Pfam domain/function: PF01979 Amidohydro_1; SSF51338 Metalo_hydrolase

EC number: =3.5.4.28

Molecular weight: Translated: 45488; Mature: 45488

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: BINDING 86-86 BINDING 178-178 BINDING 208-208 BINDING 293-293

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDLVIRNGLCFINGEFVECSVGVDGNRITHVAKEVERGEIEIDAAGCLVMPGCFNAHTH
CCCEEEECCEEEEECCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCEEEECCCCCHHHH
AAMTLLRGYAEGLPLREWLEKVWEVEARLDEDAVYWGTMLACVEMLKSGVTAFADMYIHM
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAVAEAVGESGMRAVLGYGMADRGDEERARKELEIGLEFAEKWNGGFEGRVTTMLAPHAP
HHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
YTCSPEFLKVVSDASKDKGFLKHIHVSETLWEVKEVRERYGKRPVEFLDSIGFLDSSTVL
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHH
AHAVWLSEAEMKILAERGVSVAHCPTSNLKLSSGIAKVSELLEMGVNVGIGTDGAASNNM
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
LSVLSDARVGALLQNLRGRTLKPGHWLEMATEGGYRAYNLKGGRIEEGYLADIVVFSKTC
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHH
RNAPMHDPAAMLYVENQALHAVVDGVLVMEDGILVNVEEEKVIEKAEETALELVGG
CCCCCCCCEEEEEECCCHHHHHHCCEEEEECCEEEEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHDLVIRNGLCFINGEFVECSVGVDGNRITHVAKEVERGEIEIDAAGCLVMPGCFNAHTH
CCCEEEECCEEEEECCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCEEEECCCCCHHHH
AAMTLLRGYAEGLPLREWLEKVWEVEARLDEDAVYWGTMLACVEMLKSGVTAFADMYIHM
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAVAEAVGESGMRAVLGYGMADRGDEERARKELEIGLEFAEKWNGGFEGRVTTMLAPHAP
HHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
YTCSPEFLKVVSDASKDKGFLKHIHVSETLWEVKEVRERYGKRPVEFLDSIGFLDSSTVL
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHH
AHAVWLSEAEMKILAERGVSVAHCPTSNLKLSSGIAKVSELLEMGVNVGIGTDGAASNNM
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
LSVLSDARVGALLQNLRGRTLKPGHWLEMATEGGYRAYNLKGGRIEEGYLADIVVFSKTC
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHH
RNAPMHDPAAMLYVENQALHAVVDGVLVMEDGILVNVEEEKVIEKAEETALELVGG
CCCCCCCCEEEEEECCCHHHHHHCCEEEEECCEEEEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475