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The map label for this gene is gspE-3

Identifier: 11498601

GI number: 11498601

Start: 898194

End: 899903

Strand: Reverse

Name: gspE-3

Synonym: AF0996

Alternate gene names: 11498601

Gene position: 899903-898194 (Counterclockwise)

Preceding gene: 11498602

Following gene: 11498600

Centisome position: 41.31

GC content: 50.47

Gene sequence:

>1710_bases
ATGCCAAAAATCAATTTCAGGCAACTGAAATTCAGGGGAAAAGAGCAGGAGGAGTCGGAGGAAGAAGAGGCTGAGCAGCT
TGCCGACTACGAAAAAGAAGCTCTGGAGGCGATAGAGGAGGTAAAGCAAAAGGAGGAAATCCTCAAAGTTAAGCGTGAAA
AGAAGAAGATCATCGGATTGCCAAAAAGGAAGGAGTACAGTGTTGAAGAGCATGGGGAGCTTGTGGAGTACTCTCCGCCA
GTTGGGTGGAGCATCGTCGAGGAGTACTGGCTGCTTGAGCCGTACTGCAAGGCTTTTATTATTTACAACGAGGAGGAGCA
GGAGTACAGGTATCAGGTCGTCGAACCGAGATTAGACCCATACGAAACGGAGGTTTACGGTCACCTCTCTGTCCTGCTCA
AAGATAAGCTTGAGGAAAATTTCTTTGAGGATGGGAAGGTTGCCAAGGAAGTCGTTCTCAAGGAAACCTTTGACGATTTG
ATCAGCGAAGTGGGGTTCGATTTGGAAGACCGCTCCTATTACAAAATATGGTACTACATCATGAGAGATTTTCTTTACTA
CGACAAGATAACTCCGCTAATGCTGGACAAGATGCTTGAGGACATCTCATGCAATGGTGTTCGGAAGTACATGTATGTCT
TCCACAGAAACTACACAAACCTCCGCACCAACGTCATCTTCGACGATCCGGATGAGCTTGACAGCTTCGTTGTCAACCTC
GCCCAGAAGTGCGGAAAGCACATCAGCATCGCAGAGCCAATGGTTGACGCGACAATGCCCGACGGCAGCAGAATCCAGAT
GACTTTGGGGAGGGAGGTGACCGACCACGGCTCGACCTTCACCATAAGAAAGTTCCGCGAAGAGCCCGTCACTCCCATCG
ACCTAATAGCATGGAAAACCTTCAGCAGCGAGCAGATGGCATATCTATGGCTCTGCATCGAGAACAAGAAGTCCCTTATC
TTTGCCGGCGGCACTGCGAGCGGTAAAACCACTTCGATGAACGCCATCTCGCTCTTCATCCCCCGCCGCTCCAAGATAGT
GACAATTGAAGACACAAGAGAGCTTATGCTCCCCCACGAGAACTGGATTCCTGCTGTAACAAGGGATGCCTTCCACGGGG
AGAAGGGAGCAGTTGACATGTACGATTTGCTGAGAGCGGCTTTGAGGCAGAGGCCTGAATACATAATAGTCGGTGAGGTT
AGAGGGAGGGAGGCTTTAACGCTCTTCCAGGCGATGGCTACAGGCCACACAACCTACTCAACACTGCACGCTGACAGCAT
AAGCGGTGCGATACACAGGCTTGAAAACCCGCCAATAGGAGTGCCGAGGCCGATGCTCGAGGCTCTTGACATTATAAGCA
TCCAGGCCCAAACTTACGTTGGGGACAGGAGAGTTAGGAGGAACATGGAGATTGCGGAGATTGTCGGTTTGGATGCGCAC
ACAAAGATGCTGAGAACCTCAACCGTATTCCAGTGGGACAGCGTTAAGGACGAGCACGCGATGGTTGGAACTTCCAAAGC
TCTGGAGGAGATAAGGAGGCAGAGAGGGTGGAGTGTGAGGGAGCTGAACGAGGAGCTTGAGAGGAGGAGGAGAGTGCTGG
AGTTTATGCTCGAGCACAACGTAAGGGATTTCAAGAGGGTGAGCAACATAATTCACACCTACCAGACCAAGCCGGATAAA
ATTATGGAAGCCATTTCAAAGGAGGGGTAA

Upstream 100 bases:

>100_bases
AAAGGCTGAGGAAACGGCACTCGAGCTGGTAGGTGGTTAGCCAGAATGCTTGCAAAAATAGGAGAAAATTTTAATTATTT
CTTATGTTAACATCATAACA

Downstream 100 bases:

>100_bases
TATGTTTAAGGAGGCGAACAGGCTTACTTACATAGGATACAGGCTTTTTGCTGAAGATATCATGAAGAAAGAATCGAAGT
ACTTCGAGCTTGAGAGGTCC

Product: type II secretion system protein (gspE-3)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MPKINFRQLKFRGKEQEESEEEEAEQLADYEKEALEAIEEVKQKEEILKVKREKKKIIGLPKRKEYSVEEHGELVEYSPP
VGWSIVEEYWLLEPYCKAFIIYNEEEQEYRYQVVEPRLDPYETEVYGHLSVLLKDKLEENFFEDGKVAKEVVLKETFDDL
ISEVGFDLEDRSYYKIWYYIMRDFLYYDKITPLMLDKMLEDISCNGVRKYMYVFHRNYTNLRTNVIFDDPDELDSFVVNL
AQKCGKHISIAEPMVDATMPDGSRIQMTLGREVTDHGSTFTIRKFREEPVTPIDLIAWKTFSSEQMAYLWLCIENKKSLI
FAGGTASGKTTSMNAISLFIPRRSKIVTIEDTRELMLPHENWIPAVTRDAFHGEKGAVDMYDLLRAALRQRPEYIIVGEV
RGREALTLFQAMATGHTTYSTLHADSISGAIHRLENPPIGVPRPMLEALDIISIQAQTYVGDRRVRRNMEIAEIVGLDAH
TKMLRTSTVFQWDSVKDEHAMVGTSKALEEIRRQRGWSVRELNEELERRRRVLEFMLEHNVRDFKRVSNIIHTYQTKPDK
IMEAISKEG

Sequences:

>Translated_569_residues
MPKINFRQLKFRGKEQEESEEEEAEQLADYEKEALEAIEEVKQKEEILKVKREKKKIIGLPKRKEYSVEEHGELVEYSPP
VGWSIVEEYWLLEPYCKAFIIYNEEEQEYRYQVVEPRLDPYETEVYGHLSVLLKDKLEENFFEDGKVAKEVVLKETFDDL
ISEVGFDLEDRSYYKIWYYIMRDFLYYDKITPLMLDKMLEDISCNGVRKYMYVFHRNYTNLRTNVIFDDPDELDSFVVNL
AQKCGKHISIAEPMVDATMPDGSRIQMTLGREVTDHGSTFTIRKFREEPVTPIDLIAWKTFSSEQMAYLWLCIENKKSLI
FAGGTASGKTTSMNAISLFIPRRSKIVTIEDTRELMLPHENWIPAVTRDAFHGEKGAVDMYDLLRAALRQRPEYIIVGEV
RGREALTLFQAMATGHTTYSTLHADSISGAIHRLENPPIGVPRPMLEALDIISIQAQTYVGDRRVRRNMEIAEIVGLDAH
TKMLRTSTVFQWDSVKDEHAMVGTSKALEEIRRQRGWSVRELNEELERRRRVLEFMLEHNVRDFKRVSNIIHTYQTKPDK
IMEAISKEG
>Mature_568_residues
PKINFRQLKFRGKEQEESEEEEAEQLADYEKEALEAIEEVKQKEEILKVKREKKKIIGLPKRKEYSVEEHGELVEYSPPV
GWSIVEEYWLLEPYCKAFIIYNEEEQEYRYQVVEPRLDPYETEVYGHLSVLLKDKLEENFFEDGKVAKEVVLKETFDDLI
SEVGFDLEDRSYYKIWYYIMRDFLYYDKITPLMLDKMLEDISCNGVRKYMYVFHRNYTNLRTNVIFDDPDELDSFVVNLA
QKCGKHISIAEPMVDATMPDGSRIQMTLGREVTDHGSTFTIRKFREEPVTPIDLIAWKTFSSEQMAYLWLCIENKKSLIF
AGGTASGKTTSMNAISLFIPRRSKIVTIEDTRELMLPHENWIPAVTRDAFHGEKGAVDMYDLLRAALRQRPEYIIVGEVR
GREALTLFQAMATGHTTYSTLHADSISGAIHRLENPPIGVPRPMLEALDIISIQAQTYVGDRRVRRNMEIAEIVGLDAHT
KMLRTSTVFQWDSVKDEHAMVGTSKALEEIRRQRGWSVRELNEELERRRRVLEFMLEHNVRDFKRVSNIIHTYQTKPDKI
MEAISKEG

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG0630

COG function: function code NU; Type IV secretory pathway, VirB11 components, and related ATPases involved in archaeal flagella biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=121, Percent_Identity=39.6694214876033, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 66522; Mature: 66391

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKINFRQLKFRGKEQEESEEEEAEQLADYEKEALEAIEEVKQKEEILKVKREKKKIIGL
CCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKRKEYSVEEHGELVEYSPPVGWSIVEEYWLLEPYCKAFIIYNEEEQEYRYQVVEPRLDP
CCCCCCCHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHEECCCCCC
YETEVYGHLSVLLKDKLEENFFEDGKVAKEVVLKETFDDLISEVGFDLEDRSYYKIWYYI
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
MRDFLYYDKITPLMLDKMLEDISCNGVRKYMYVFHRNYTNLRTNVIFDDPDELDSFVVNL
HHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHH
AQKCGKHISIAEPMVDATMPDGSRIQMTLGREVTDHGSTFTIRKFREEPVTPIDLIAWKT
HHHCCCCCCCCCCHHHCCCCCCCEEEEEECCEECCCCCEEEEEEHHCCCCCCCHHEEEEC
FSSEQMAYLWLCIENKKSLIFAGGTASGKTTSMNAISLFIPRRSKIVTIEDTRELMLPHE
CCCCCEEEEEEEEECCCEEEEECCCCCCCCCCCCEEEEEECCCCEEEEEECCHHHCCCCC
NWIPAVTRDAFHGEKGAVDMYDLLRAALRQRPEYIIVGEVRGREALTLFQAMATGHTTYS
CCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCHHH
TLHADSISGAIHRLENPPIGVPRPMLEALDIISIQAQTYVGDRRVRRNMEIAEIVGLDAH
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEHHHHHHHHHHHCCCCHHHHHCCCHH
TKMLRTSTVFQWDSVKDEHAMVGTSKALEEIRRQRGWSVRELNEELERRRRVLEFMLEHN
HHHHHHHHEEEECCCCCCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
VRDFKRVSNIIHTYQTKPDKIMEAISKEG
HHHHHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
PKINFRQLKFRGKEQEESEEEEAEQLADYEKEALEAIEEVKQKEEILKVKREKKKIIGL
CCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKRKEYSVEEHGELVEYSPPVGWSIVEEYWLLEPYCKAFIIYNEEEQEYRYQVVEPRLDP
CCCCCCCHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHEECCCCCC
YETEVYGHLSVLLKDKLEENFFEDGKVAKEVVLKETFDDLISEVGFDLEDRSYYKIWYYI
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
MRDFLYYDKITPLMLDKMLEDISCNGVRKYMYVFHRNYTNLRTNVIFDDPDELDSFVVNL
HHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHH
AQKCGKHISIAEPMVDATMPDGSRIQMTLGREVTDHGSTFTIRKFREEPVTPIDLIAWKT
HHHCCCCCCCCCCHHHCCCCCCCEEEEEECCEECCCCCEEEEEEHHCCCCCCCHHEEEEC
FSSEQMAYLWLCIENKKSLIFAGGTASGKTTSMNAISLFIPRRSKIVTIEDTRELMLPHE
CCCCCEEEEEEEEECCCEEEEECCCCCCCCCCCCEEEEEECCCCEEEEEECCHHHCCCCC
NWIPAVTRDAFHGEKGAVDMYDLLRAALRQRPEYIIVGEVRGREALTLFQAMATGHTTYS
CCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCHHH
TLHADSISGAIHRLENPPIGVPRPMLEALDIISIQAQTYVGDRRVRRNMEIAEIVGLDAH
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEHHHHHHHHHHHCCCCHHHHHCCCHH
TKMLRTSTVFQWDSVKDEHAMVGTSKALEEIRRQRGWSVRELNEELERRRRVLEFMLEHN
HHHHHHHHEEEECCCCCCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
VRDFKRVSNIIHTYQTKPDKIMEAISKEG
HHHHHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]