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The map label for this gene is hisA-2

Identifier: 11498591

GI number: 11498591

Start: 886976

End: 887692

Strand: Reverse

Name: hisA-2

Synonym: AF0986

Alternate gene names: 11498591

Gene position: 887692-886976 (Counterclockwise)

Preceding gene: 11498592

Following gene: 11498590

Centisome position: 40.75

GC content: 49.23

Gene sequence:

>717_bases
ATGTTTCGAGTTATTCCCGCCATAGACCTCAAGGATGGGAAGGTTGTAAGGTTGAGGCAGGGTAAAGAGGATGAGGTAAC
CTTTGAGGCTACCAACCCCATTGAAGTTGCAAAGCAATGGGTTGAGATAGGAGCGAAGGTTTTGCACGTCATTGATTTGA
GCGGCGCCTTTCAGGGAAGGCTGAGGCACGAGGAGATTATCGCTGAGATTGCGAAAATGGCAGAGGTGCAGGTTGGTGGG
GGAATAAGGGATTTCAGAGTTGCTGAGAGACTTTTTGAGCTTGGTGTGGACAGGGTTATTTTCGGGACGATTGCTGTAGA
GAGGGTTGAGGAGCTCAGAGAGTTCGCCAAGAAATGGAAGGGGAGGGTCATGGTTGCCATAGACTCGAAGAAGGGCAGAG
TTGCTGTGAAGGGCTGGAAGGAGGTGGTGGAGCTTACACCCGTGCAGCTTGCAGAGCTCTATGATGACCTCGACGTTTCA
TTCCTCTACACCAACATCGATGTTGAGGGGCTGGTTTCCGGAATAGAGAGGGAAAGAATTGAGGAAGTTGTGAAAAGCCT
CAGAAATCCGGTTTACGTCGCGGGAGGGATTAGCAGCATAGAGGACATCAGATTCGCAAAGAGGGTTGGGGCTGCAGGAG
TTGTGATTGGCTCGGCTTTGTACACTAAAAAGCTTAAATTTGAGGAAGCAATAAAGGTGGAGTATGAAAAAGTTTAG

Upstream 100 bases:

>100_bases
ATTGCTCTTCGGCAGTGTTGCTCAGCAGGTTATACTGCACGCAAAGCAGCCTGTTGTCTGCATCAAATAATTTTTTAACC
TTTTCTTCCCACTCCTTAAG

Downstream 100 bases:

>100_bases
CAGGAAAACGGAGGAGACAGAGGTGGAAGTCGTTCTTGGAGAAGAGCTGAAAGTTGATACTGGCATCGCTTTTCTCGACC
ACATGCTGAAAACGCTTTCA

Product: phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (hisA-2)

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MFRVIPAIDLKDGKVVRLRQGKEDEVTFEATNPIEVAKQWVEIGAKVLHVIDLSGAFQGRLRHEEIIAEIAKMAEVQVGG
GIRDFRVAERLFELGVDRVIFGTIAVERVEELREFAKKWKGRVMVAIDSKKGRVAVKGWKEVVELTPVQLAELYDDLDVS
FLYTNIDVEGLVSGIERERIEEVVKSLRNPVYVAGGISSIEDIRFAKRVGAAGVVIGSALYTKKLKFEEAIKVEYEKV

Sequences:

>Translated_238_residues
MFRVIPAIDLKDGKVVRLRQGKEDEVTFEATNPIEVAKQWVEIGAKVLHVIDLSGAFQGRLRHEEIIAEIAKMAEVQVGG
GIRDFRVAERLFELGVDRVIFGTIAVERVEELREFAKKWKGRVMVAIDSKKGRVAVKGWKEVVELTPVQLAELYDDLDVS
FLYTNIDVEGLVSGIERERIEEVVKSLRNPVYVAGGISSIEDIRFAKRVGAAGVVIGSALYTKKLKFEEAIKVEYEKV
>Mature_238_residues
MFRVIPAIDLKDGKVVRLRQGKEDEVTFEATNPIEVAKQWVEIGAKVLHVIDLSGAFQGRLRHEEIIAEIAKMAEVQVGG
GIRDFRVAERLFELGVDRVIFGTIAVERVEELREFAKKWKGRVMVAIDSKKGRVAVKGWKEVVELTPVQLAELYDDLDVS
FLYTNIDVEGLVSGIERERIEEVVKSLRNPVYVAGGISSIEDIRFAKRVGAAGVVIGSALYTKKLKFEEAIKVEYEKV

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=32.0833333333333, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_ARCFU (O29276)

Other databases:

- EMBL:   AE000782
- PIR:   B69373
- RefSeq:   NP_069819.1
- ProteinModelPortal:   O29276
- GeneID:   1484209
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0986
- NMPDR:   fig|224325.1.peg.978
- TIGR:   AF_0986
- HOGENOM:   HBG541613
- OMA:   CARYVVT
- ProtClustDB:   CLSK2746700
- BioCyc:   AFUL224325:AF_0986-MONOMER
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26698; Mature: 26698

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: ACT_SITE 9-9 ACT_SITE 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRVIPAIDLKDGKVVRLRQGKEDEVTFEATNPIEVAKQWVEIGAKVLHVIDLSGAFQGR
CCEEECCEECCCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHCC
LRHEEIIAEIAKMAEVQVGGGIRDFRVAERLFELGVDRVIFGTIAVERVEELREFAKKWK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHC
GRVMVAIDSKKGRVAVKGWKEVVELTPVQLAELYDDLDVSFLYTNIDVEGLVSGIERERI
CEEEEEEECCCCEEEHHHHHHHHHHCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHH
EEVVKSLRNPVYVAGGISSIEDIRFAKRVGAAGVVIGSALYTKKLKFEEAIKVEYEKV
HHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MFRVIPAIDLKDGKVVRLRQGKEDEVTFEATNPIEVAKQWVEIGAKVLHVIDLSGAFQGR
CCEEECCEECCCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHCC
LRHEEIIAEIAKMAEVQVGGGIRDFRVAERLFELGVDRVIFGTIAVERVEELREFAKKWK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHC
GRVMVAIDSKKGRVAVKGWKEVVELTPVQLAELYDDLDVSFLYTNIDVEGLVSGIERERI
CEEEEEEECCCCEEEHHHHHHHHHHCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHH
EEVVKSLRNPVYVAGGISSIEDIRFAKRVGAAGVVIGSALYTKKLKFEEAIKVEYEKV
HHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475