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The map label for this gene is hisB

Identifier: 11498590

GI number: 11498590

Start: 886453

End: 886989

Strand: Reverse

Name: hisB

Synonym: AF0985

Alternate gene names: 11498590

Gene position: 886989-886453 (Counterclockwise)

Preceding gene: 11498591

Following gene: 11498589

Centisome position: 40.72

GC content: 49.72

Gene sequence:

>537_bases
ATGAAAAAGTTTAGCAGGAAAACGGAGGAGACAGAGGTGGAAGTCGTTCTTGGAGAAGAGCTGAAAGTTGATACTGGCAT
CGCTTTTCTCGACCACATGCTGAAAACGCTTTCAAGGCACTCCGGGATAAAGCTGAGAGTGAAGGCAAAGGGAGACCTTG
AGCACCACGTTATAGAGGATGTTGCCATCGCGCTCGGAAAGGCTCTTGCCGGGGTGGATAAAAAGGGTTTGGAGAGGTTT
GGAGATGCCATCGTTCCAATGGATGATGCTGTAGCAATCTGCGGTCTCGACTTCAGCGGGAGAGGCGTGCTTGTGGTGGA
GGGGACTTTTGGGGATGGTGAGATGAGGGAAGAGGACTTTCTCCACTTCCTCGACACCCTCTGCAGAAATGCTGGCTTGA
ACGTTTACCTTAGCGTTAAGGGGAGCAATTCCCACCACAAAATGGAGGCTGCGGTTAAGGCAGTTGCTATATCGCTGAAA
AAAGCTTTGACAAAAAATGGAAATGATTACAGAAGCGCGAAGGGAGTTCTGGACTGA

Upstream 100 bases:

>100_bases
GACATCAGATTCGCAAAGAGGGTTGGGGCTGCAGGAGTTGTGATTGGCTCGGCTTTGTACACTAAAAAGCTTAAATTTGA
GGAAGCAATAAAGGTGGAGT

Downstream 100 bases:

>100_bases
GATGCAGTATGATAGAGGTTAGAAATCTCTCGAAGCGATTCGGAAGGAAACTCGTCTTAAAGAACCTCAATTTCGAAGTT
GACGCTGGAAGGGTAGCTGT

Product: imidazoleglycerol-phosphate dehydrogenase/histidinol-phosphatase (hisB)

Products: NA

Alternate protein names: IGPD

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MKKFSRKTEETEVEVVLGEELKVDTGIAFLDHMLKTLSRHSGIKLRVKAKGDLEHHVIEDVAIALGKALAGVDKKGLERF
GDAIVPMDDAVAICGLDFSGRGVLVVEGTFGDGEMREEDFLHFLDTLCRNAGLNVYLSVKGSNSHHKMEAAVKAVAISLK
KALTKNGNDYRSAKGVLD

Sequences:

>Translated_178_residues
MKKFSRKTEETEVEVVLGEELKVDTGIAFLDHMLKTLSRHSGIKLRVKAKGDLEHHVIEDVAIALGKALAGVDKKGLERF
GDAIVPMDDAVAICGLDFSGRGVLVVEGTFGDGEMREEDFLHFLDTLCRNAGLNVYLSVKGSNSHHKMEAAVKAVAISLK
KALTKNGNDYRSAKGVLD
>Mature_178_residues
MKKFSRKTEETEVEVVLGEELKVDTGIAFLDHMLKTLSRHSGIKLRVKAKGDLEHHVIEDVAIALGKALAGVDKKGLERF
GDAIVPMDDAVAICGLDFSGRGVLVVEGTFGDGEMREEDFLHFLDTLCRNAGLNVYLSVKGSNSHHKMEAAVKAVAISLK
KALTKNGNDYRSAKGVLD

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the imidazoleglycerol-phosphate dehydratase family

Homologues:

Organism=Escherichia coli, GI87082027, Length=184, Percent_Identity=39.6739130434783, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324776, Length=184, Percent_Identity=36.4130434782609, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS7_ARCFU (O29277)

Other databases:

- EMBL:   AE000782
- PIR:   A69373
- RefSeq:   NP_069818.1
- ProteinModelPortal:   O29277
- SMR:   O29277
- GeneID:   1484208
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0985
- NMPDR:   fig|224325.1.peg.977
- TIGR:   AF_0985
- HOGENOM:   HBG289010
- OMA:   YYMNSTA
- ProtClustDB:   CLSK2746699
- BioCyc:   AFUL224325:AF_0985-MONOMER
- BRENDA:   4.2.1.19
- GO:   GO:0005737
- HAMAP:   MF_00076
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR020568

Pfam domain/function: PF00475 IGPD; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =4.2.1.19

Molecular weight: Translated: 19336; Mature: 19336

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00954 IGP_DEHYDRATASE_1; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFSRKTEETEVEVVLGEELKVDTGIAFLDHMLKTLSRHSGIKLRVKAKGDLEHHVIED
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
VAIALGKALAGVDKKGLERFGDAIVPMDDAVAICGLDFSGRGVLVVEGTFGDGEMREEDF
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCHHHH
LHFLDTLCRNAGLNVYLSVKGSNSHHKMEAAVKAVAISLKKALTKNGNDYRSAKGVLD
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCC
>Mature Secondary Structure
MKKFSRKTEETEVEVVLGEELKVDTGIAFLDHMLKTLSRHSGIKLRVKAKGDLEHHVIED
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
VAIALGKALAGVDKKGLERFGDAIVPMDDAVAICGLDFSGRGVLVVEGTFGDGEMREEDF
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCHHHH
LHFLDTLCRNAGLNVYLSVKGSNSHHKMEAAVKAVAISLKKALTKNGNDYRSAKGVLD
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475