Click here to switch to the map view.

The map label for this gene is ppsA

Identifier: 11498317

GI number: 11498317

Start: 640271

End: 642532

Strand: Reverse

Name: ppsA

Synonym: AF0710

Alternate gene names: 11498317

Gene position: 642532-640271 (Counterclockwise)

Preceding gene: 11498320

Following gene: 11498313

Centisome position: 29.5

GC content: 51.19

Gene sequence:

>2262_bases
ATGCCGGTACTCTGGTTGGCGGATGTTGATAAAAACGATATTCCTCTTGTTGGTGGGAAGGGTGCGAATCTTGGTGAACT
TTTAAGAGCTGAAATTCCGGTTCCTGATGGTTTCGTTGTTGACGCTCGAACGTTTCGCGAATTCATTCAGAAAACAGGGA
TAGCTGAGAAAATTTACTCCCTGCTAAGGGAACTCGATGTTGAGGATACGGAAAAGCTGGATGCTGTTTCGAGGGAAATC
AGGGAAATCATCGAAAAGACGGAGATGCCTGAAGACATTGAGAGGGAGATCAGAGAGGCGTACAGGAAGCTCTGCGAGGA
GGAGGGAAAGGAAGTTTACGTTGCGGTCAGAAGCTCAGCAACCGCAGAAGATTTGCCGGATGCAAGCTTTGCGGGGCAGC
AGGAGACTTATCTGAACGTTGTTGGCGAGGACGAGGTTGTTGAGAAGGTCAAGAAATGCTGGGGGAGTCTTTTCACACCT
AGGGCCATTTACTATAGAGTCCAGAAGGGCTTCAGGCATGAGGACGTCAGCATCGCCGTCGTTGTGCAGAAGATGGTGAA
CAGCGAGAAGAGCGGAGTAATGTTCACGTCCCATCCGGTTAGCGGGGAGAAGAAGTGCATCATCGAGGCGGTTTTCGGCC
TGGGAGAGGCTATTGTAAGCGGTCTCGTCACTCCCGACACGTACGTTTACGACAGAGTGAAGAGAAAGATAGAGGAGGTC
AAGATTGGTGAGAAGAAGTTCATGCTTACGAGAAAAGACGGGAAGACCGTTAAGGTCGAGCTTCCACCCGAGAAAGCAAA
CGAGAGGGTGCTGAGCGATGAGGAGATAGAAAAGCTCGTAACTCTTGGAGAGCTGATAGAGGACCACTACGGCAAGCCGC
AGGACGTCGAGTGGGCTATTGAGGGGGGCAAGATATACATAGTCCAGTCGAGGCCGATCACAACCATAAGAAAAGAGAAA
AAGGAGGCTGAAGAAGAGGTTTCGGAAGAGGCAGAGGGCAAAATCCTTCTGAAGGGGCTTGGAGCATCTCCGGGTATCGC
CTCGGGTAAGGTGAAGGTGATTTTCAGCGAGAAAGAGATTTCCAAGGTTGAAGAGGGAGACATACTGGTCACAACGATGA
CCACACCAGACATGGTCCCGGCGATGAAGAGAGCAGCCGCCATTGTGACGGACGAGGGAGGTATGACCTGCCATGCAGCC
ATAGTTTCAAGAGAACTCGGAGTGCCGGCGGTTGTTGGAACTAAAGTCGCAACTAAGGTTCTGAAAGACGGAATGGTTGT
AACCGTTGACGGTGAGAAGGGAATCGTTTACGAGGGCAGAATTGAGAAGAAGGAGGAACCAAAACCGGTAGTGGCTTCCG
CTCCAATCATAACCGCGACAGAGGTGAAGGTGAACATCTCAATTCCCGACGTGGCGGAGAGGGTTGCGAGGGAGACAAAT
GCGGATGGGGTCGGTTTGTTCAGAATCGAGCACATGGTTCTCGGGCTCGAAAAGCATCCGATGAAGTTCATAAGAGATGG
TGAGATTGACAGGTACATCGATTTGCTCTATCAGGAAATGAAGAAGGTTGTCAAGGCGTTCTATCCCAAGCCGGTTTGGA
TCAGAACCATCGACGCTCCAACGGATGAGTTCAGGGCTATGGAGGGTGGAGAGGACGAGCCTATTGAGGCCAATCCGATG
CTCGGATTCAGAGGAATCAGAAGGGACCTGGCGGAGGAGGAGCACTTCAGGGCCGAGATGAGGGCGATAAAGAAGCTTGT
GGACGAGGGATACACCAACGTTGGGGTGATGCTTCCGCTGATAACCTCTCCGGAGGAGGTCAAAAGGGCTAAGGAGATAG
CCATCAGCGAGGGATTGCCGCTGGACAAAATTGAGTTTGGAGTGATGGTTGAAACGCCGGCAGCAGCGCTAATACTTGAG
GATATCATTAAAGAGGGCATAGACTTCGTCAGCCTAGGAACAAACGATTTGACGCAGTACACTCTGGCGGTGGACAGGAA
CAACGAGAACGTCGCATACCTTTACAACGAAACCCATCCTGCCGTGCTCAAGCTCATCGAGAGAACAATAAAGGTTTGCA
AGGAGCACGGAGTGAAGAGCTCGATCTGCGGTCAGGCTGGCAGCTATCCGCACGTCGTTGAGAAGCTGGTCGAGTTTGGC
ATCGACAGTGTTTCGGCAAATCCCGATGCGGTCCAAAGGATAAGGGAAGTCGTGGCAAGAGCTGAGAAAAGGATAATTCT
GGAAAAGCTCAGAAAGATTTAG

Upstream 100 bases:

>100_bases
CACCATTGGCATCTATGATTTCCTGCTTAATATATCGCTTGCGATTCCGCTCCACCTAAAATTTTTTAAGGCTTTGGCAA
ACTCAGAACGAGGTGAGTAA

Downstream 100 bases:

>100_bases
CCGTAGGCAATTATCTTTCTTGCCTCCATTTTTCCAATTTTTGAATTCCCTTCGATCAAAAGTCTGTTTCCTGCCCTTAT
TTCGTCGATTTCGCAGTTCT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase

Number of amino acids: Translated: 753; Mature: 752

Protein sequence:

>753_residues
MPVLWLADVDKNDIPLVGGKGANLGELLRAEIPVPDGFVVDARTFREFIQKTGIAEKIYSLLRELDVEDTEKLDAVSREI
REIIEKTEMPEDIEREIREAYRKLCEEEGKEVYVAVRSSATAEDLPDASFAGQQETYLNVVGEDEVVEKVKKCWGSLFTP
RAIYYRVQKGFRHEDVSIAVVVQKMVNSEKSGVMFTSHPVSGEKKCIIEAVFGLGEAIVSGLVTPDTYVYDRVKRKIEEV
KIGEKKFMLTRKDGKTVKVELPPEKANERVLSDEEIEKLVTLGELIEDHYGKPQDVEWAIEGGKIYIVQSRPITTIRKEK
KEAEEEVSEEAEGKILLKGLGASPGIASGKVKVIFSEKEISKVEEGDILVTTMTTPDMVPAMKRAAAIVTDEGGMTCHAA
IVSRELGVPAVVGTKVATKVLKDGMVVTVDGEKGIVYEGRIEKKEEPKPVVASAPIITATEVKVNISIPDVAERVARETN
ADGVGLFRIEHMVLGLEKHPMKFIRDGEIDRYIDLLYQEMKKVVKAFYPKPVWIRTIDAPTDEFRAMEGGEDEPIEANPM
LGFRGIRRDLAEEEHFRAEMRAIKKLVDEGYTNVGVMLPLITSPEEVKRAKEIAISEGLPLDKIEFGVMVETPAAALILE
DIIKEGIDFVSLGTNDLTQYTLAVDRNNENVAYLYNETHPAVLKLIERTIKVCKEHGVKSSICGQAGSYPHVVEKLVEFG
IDSVSANPDAVQRIREVVARAEKRIILEKLRKI

Sequences:

>Translated_753_residues
MPVLWLADVDKNDIPLVGGKGANLGELLRAEIPVPDGFVVDARTFREFIQKTGIAEKIYSLLRELDVEDTEKLDAVSREI
REIIEKTEMPEDIEREIREAYRKLCEEEGKEVYVAVRSSATAEDLPDASFAGQQETYLNVVGEDEVVEKVKKCWGSLFTP
RAIYYRVQKGFRHEDVSIAVVVQKMVNSEKSGVMFTSHPVSGEKKCIIEAVFGLGEAIVSGLVTPDTYVYDRVKRKIEEV
KIGEKKFMLTRKDGKTVKVELPPEKANERVLSDEEIEKLVTLGELIEDHYGKPQDVEWAIEGGKIYIVQSRPITTIRKEK
KEAEEEVSEEAEGKILLKGLGASPGIASGKVKVIFSEKEISKVEEGDILVTTMTTPDMVPAMKRAAAIVTDEGGMTCHAA
IVSRELGVPAVVGTKVATKVLKDGMVVTVDGEKGIVYEGRIEKKEEPKPVVASAPIITATEVKVNISIPDVAERVARETN
ADGVGLFRIEHMVLGLEKHPMKFIRDGEIDRYIDLLYQEMKKVVKAFYPKPVWIRTIDAPTDEFRAMEGGEDEPIEANPM
LGFRGIRRDLAEEEHFRAEMRAIKKLVDEGYTNVGVMLPLITSPEEVKRAKEIAISEGLPLDKIEFGVMVETPAAALILE
DIIKEGIDFVSLGTNDLTQYTLAVDRNNENVAYLYNETHPAVLKLIERTIKVCKEHGVKSSICGQAGSYPHVVEKLVEFG
IDSVSANPDAVQRIREVVARAEKRIILEKLRKI
>Mature_752_residues
PVLWLADVDKNDIPLVGGKGANLGELLRAEIPVPDGFVVDARTFREFIQKTGIAEKIYSLLRELDVEDTEKLDAVSREIR
EIIEKTEMPEDIEREIREAYRKLCEEEGKEVYVAVRSSATAEDLPDASFAGQQETYLNVVGEDEVVEKVKKCWGSLFTPR
AIYYRVQKGFRHEDVSIAVVVQKMVNSEKSGVMFTSHPVSGEKKCIIEAVFGLGEAIVSGLVTPDTYVYDRVKRKIEEVK
IGEKKFMLTRKDGKTVKVELPPEKANERVLSDEEIEKLVTLGELIEDHYGKPQDVEWAIEGGKIYIVQSRPITTIRKEKK
EAEEEVSEEAEGKILLKGLGASPGIASGKVKVIFSEKEISKVEEGDILVTTMTTPDMVPAMKRAAAIVTDEGGMTCHAAI
VSRELGVPAVVGTKVATKVLKDGMVVTVDGEKGIVYEGRIEKKEEPKPVVASAPIITATEVKVNISIPDVAERVARETNA
DGVGLFRIEHMVLGLEKHPMKFIRDGEIDRYIDLLYQEMKKVVKAFYPKPVWIRTIDAPTDEFRAMEGGEDEPIEANPML
GFRGIRRDLAEEEHFRAEMRAIKKLVDEGYTNVGVMLPLITSPEEVKRAKEIAISEGLPLDKIEFGVMVETPAAALILED
IIKEGIDFVSLGTNDLTQYTLAVDRNNENVAYLYNETHPAVLKLIERTIKVCKEHGVKSSICGQAGSYPHVVEKLVEFGI
DSVSANPDAVQRIREVVARAEKRIILEKLRKI

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1787994, Length=782, Percent_Identity=38.2352941176471, Blast_Score=464, Evalue=1e-131,
Organism=Escherichia coli, GI1788756, Length=412, Percent_Identity=33.9805825242718, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1789193, Length=391, Percent_Identity=30.1790281329923, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI48994992, Length=396, Percent_Identity=27.2727272727273, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788726, Length=379, Percent_Identity=26.9129287598945, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17564524, Length=315, Percent_Identity=35.2380952380952, Blast_Score=156, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPSA_ARCFU (O29548)

Other databases:

- EMBL:   AE000782
- PIR:   F69338
- RefSeq:   NP_069544.1
- ProteinModelPortal:   O29548
- SMR:   O29548
- GeneID:   1483928
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0710
- NMPDR:   fig|224325.1.peg.703
- TIGR:   AF_0710
- HOGENOM:   HBG284863
- OMA:   LLRAEHM
- ProtClustDB:   PRK06464
- BioCyc:   AFUL224325:AF_0710-MONOMER
- BRENDA:   2.7.9.2
- GO:   GO:0006094
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:3.20.20.60
- PRINTS:   PR01736
- TIGRFAMs:   TIGR01418

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N; SSF52009 PEP_mobile; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.9.2

Molecular weight: Translated: 83869; Mature: 83738

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 398-398 ACT_SITE 703-703 BINDING 488-488 BINDING 535-535 BINDING 631-631 BINDING 653-653 BINDING 654-654 BINDING 655-655 BINDING 656-656

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLWLADVDKNDIPLVGGKGANLGELLRAEIPVPDGFVVDARTFREFIQKTGIAEKIYS
CCEEEEECCCCCCCCEECCCCCCHHHHHHHCCCCCCCEEECHHHHHHHHHHCCHHHHHHH
LLRELDVEDTEKLDAVSREIREIIEKTEMPEDIEREIREAYRKLCEEEGKEVYVAVRSSA
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
TAEDLPDASFAGQQETYLNVVGEDEVVEKVKKCWGSLFTPRAIYYRVQKGFRHEDVSIAV
CHHCCCCCCCCCCCCHHEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHH
VVQKMVNSEKSGVMFTSHPVSGEKKCIIEAVFGLGEAIVSGLVTPDTYVYDRVKRKIEEV
HHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KIGEKKFMLTRKDGKTVKVELPPEKANERVLSDEEIEKLVTLGELIEDHYGKPQDVEWAI
CCCCCEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EGGKIYIVQSRPITTIRKEKKEAEEEVSEEAEGKILLKGLGASPGIASGKVKVIFSEKEI
ECCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECHHH
SKVEEGDILVTTMTTPDMVPAMKRAAAIVTDEGGMTCHAAIVSRELGVPAVVGTKVATKV
HHCCCCCEEEEEECCCHHHHHHHHHEEEEECCCCCEEHHHHHHHHCCCCHHHHHHHHHHH
LKDGMVVTVDGEKGIVYEGRIEKKEEPKPVVASAPIITATEVKVNISIPDVAERVARETN
HHCCEEEEEECCCCEEECCCCCCCCCCCCEEECCCEEEEEEEEEEECCHHHHHHHHHHCC
ADGVGLFRIEHMVLGLEKHPMKFIRDGEIDRYIDLLYQEMKKVVKAFYPKPVWIRTIDAP
CCCEEHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
TDEFRAMEGGEDEPIEANPMLGFRGIRRDLAEEEHFRAEMRAIKKLVDEGYTNVGVMLPL
CHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
ITSPEEVKRAKEIAISEGLPLDKIEFGVMVETPAAALILEDIIKEGIDFVSLGTNDLTQY
CCCHHHHHHHHHHHHHCCCCCCCEEECEEEECCHHHHHHHHHHHCCCCEEECCCCCCEEE
TLAVDRNNENVAYLYNETHPAVLKLIERTIKVCKEHGVKSSICGQAGSYPHVVEKLVEFG
EEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHC
IDSVSANPDAVQRIREVVARAEKRIILEKLRKI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVLWLADVDKNDIPLVGGKGANLGELLRAEIPVPDGFVVDARTFREFIQKTGIAEKIYS
CEEEEECCCCCCCCEECCCCCCHHHHHHHCCCCCCCEEECHHHHHHHHHHCCHHHHHHH
LLRELDVEDTEKLDAVSREIREIIEKTEMPEDIEREIREAYRKLCEEEGKEVYVAVRSSA
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
TAEDLPDASFAGQQETYLNVVGEDEVVEKVKKCWGSLFTPRAIYYRVQKGFRHEDVSIAV
CHHCCCCCCCCCCCCHHEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHH
VVQKMVNSEKSGVMFTSHPVSGEKKCIIEAVFGLGEAIVSGLVTPDTYVYDRVKRKIEEV
HHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KIGEKKFMLTRKDGKTVKVELPPEKANERVLSDEEIEKLVTLGELIEDHYGKPQDVEWAI
CCCCCEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EGGKIYIVQSRPITTIRKEKKEAEEEVSEEAEGKILLKGLGASPGIASGKVKVIFSEKEI
ECCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECHHH
SKVEEGDILVTTMTTPDMVPAMKRAAAIVTDEGGMTCHAAIVSRELGVPAVVGTKVATKV
HHCCCCCEEEEEECCCHHHHHHHHHEEEEECCCCCEEHHHHHHHHCCCCHHHHHHHHHHH
LKDGMVVTVDGEKGIVYEGRIEKKEEPKPVVASAPIITATEVKVNISIPDVAERVARETN
HHCCEEEEEECCCCEEECCCCCCCCCCCCEEECCCEEEEEEEEEEECCHHHHHHHHHHCC
ADGVGLFRIEHMVLGLEKHPMKFIRDGEIDRYIDLLYQEMKKVVKAFYPKPVWIRTIDAP
CCCEEHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
TDEFRAMEGGEDEPIEANPMLGFRGIRRDLAEEEHFRAEMRAIKKLVDEGYTNVGVMLPL
CHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
ITSPEEVKRAKEIAISEGLPLDKIEFGVMVETPAAALILEDIIKEGIDFVSLGTNDLTQY
CCCHHHHHHHHHHHHHCCCCCCCEEECEEEECCHHHHHHHHHHHCCCCEEECCCCCCEEE
TLAVDRNNENVAYLYNETHPAVLKLIERTIKVCKEHGVKSSICGQAGSYPHVVEKLVEFG
EEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHC
IDSVSANPDAVQRIREVVARAEKRIILEKLRKI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475