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The map label for this gene is noxB-1

Identifier: 11498067

GI number: 11498067

Start: 411030

End: 412928

Strand: Reverse

Name: noxB-1

Synonym: AF0455

Alternate gene names: 11498067

Gene position: 412928-411030 (Counterclockwise)

Preceding gene: 11498070

Following gene: 11498059

Centisome position: 18.96

GC content: 49.97

Gene sequence:

>1899_bases
ATGAAGCTTTTCGAGCCAATCGAGTTTGGAGGAATGAAGGTAAAGAACCGCATTGTAATGCCTGCTGCTGAACTCAACTA
CCACACTCCCGATGGAAGACCTACTGACAGGCTTTTGAGATTCTACGAAGAGAGGGCGAAGGGAGGGATAGGTTTTGCGG
TTGTAGGGATTGCCAAAATCGAACCCCACTTCTTTGGTGGAATAGCAGCACACTCCGACGAATTTATTCCAGACCTCAAG
AAGATAGCCGACGTCTTCCACAAGTACGACGTGAAATGCGCTTTACAGCTATGGCACCCAGGCAGATACGAAATCTCCTT
CGACCCCAACCGCCAACCAGTCGCCCCATCCCCCATACCACCACCAATCTTCACCAAGCGCACTCCCAAAGAGCTCACCA
AAGAAGAAATCCTTCAAATCGAGGAGGAGTTTGCTGATGCAGCTGTGAGGGCGAAGAAAGCTGGCTTCGATGCTGTCGAG
CTTATTGGTTCAGCAGGCTACCTCATTTCCCAGTTCTTCAGCCCCGCAACGAACAAAAGAACGGACGAGTATGGGGGCAG
CTTGGAAAACCGCACGAGGTTTGCGGTTGAGATAATTCAAAAAATCAAGGAAAAGTGCGGAGAGAGCTATCCAGTAATGA
TACGAATCCCCGGAGACGAGTTCATCGAGGGAGGGAACACAGTCAGAGAAATGAAGCAGATTGCCAAAATATTGGAGGAT
GCAGGAGTTGTAGCAATCAACGTAATGGCGGGCTGGCACGAGTCCAGAAAGCCTTTGACAACAATGCTCGTTCCCAGAGG
AGGTTTTGCTTACCTTGCAGCAGAGATAAAGAGCGCTGTAAGCGTTCCCGTTATCGCATCTCACCGCATCAATGATCCGA
TTGTGGCTGAAAAGATACTGCAGGAGGGCAAGGCAGATATGGTTGCAATGCTGAGAGCTTTGATTGCCGACCCTGAACTG
CCGAAGAAGGCCAAGGAGGGAAGGTTTGATGAGATAAGGTATTGCGTGGCCTGCAACCAGGGCTGCATGGACATGGTTAT
GCAGGCTCAGCCAGTTACATGCCTCGTTAACCCCATAGTTGGAAGGGAGGCTGAGTTTGAAAATCTGAAAGCAGAAAAAC
CAAAGAAGGTTGTTATTGTCGGAGGTGGACCGGGAGGATGCATGGCTGCAGAGATGCTTGCAAGAAAGGGGCATAAGGTA
GTTTTATTCGAGAAAACTGACAAATTGGGAGGGCAGCTCAACTTGGCTGCGAAGTCACCTTTGGGCTATGAATTTGCAGA
AGTGGGCAAGTACTTCATGAACGTCCTGCCAAAGCTTGGGGTAGAGGTGAGATACAACACGGAGGCTGATGCGGGCAAAG
TTTTAGCAGAGAACCCTGATGTTGCGGTAATAGCCGTTGGAGCTTCTCCTTTGATCCCACCAATTCCGGGAGTTGAGAAT
GCTGTAACAGCCTTCGATGTCCTCGCAGGAAGGGCAGAGGTTGGAAACAGTGTGGTGGTTATAGGCGGAGGAGGAGTTGG
ATGCGATGTTGCAGCAGAGCTTGCAAATGAGGGGAAGAAGGTTACAATTGTGGAGATGTTGCCGAAGATTGGCCAGGATA
TAGGCATCTCGACAAGATGGACCGTATTGATGTACCTGAAAGAGAAGGGAGTGGAGATGCTCACCAACACGAAGGCTGTT
GAAATCAAGCCCAATGCAGTGGTTGTGGAGCAGAATGGTGAGAGGAAAGAGTTGCAGTGTGACACTGCTGTTATAGCTGT
CGGTACAAAGCCGAACAATGGGCTGTATGACGAGCTTCAGGGCAAGGTTAGCGAGCTCTACAAGATTGGAGACTGCGTTA
AGCCCAGAAAGGCTCTCGATGCAACAAGGGAGGGAGCTGATCTGGCTTTGAAGGTTTAG

Upstream 100 bases:

>100_bases
GGTTTACATATTAGCCCTTAAGCTACTATTTTTTGGAAAATATACGTAAAATCCTACACGGATTCGAAAGGTTTATAGCT
AATCCCGCAAGCCATACAGC

Downstream 100 bases:

>100_bases
GTTTTTCGTTTTTTATTTATTTTCTGAGATTCAGTCGTTCCGAGTTTTTCTAAAACAGAAAAGGCTTCGCTGAGCTTATC
TGCAAGCCTGTCCACCTTCT

Product: NADH oxidase (noxB-1)

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MKLFEPIEFGGMKVKNRIVMPAAELNYHTPDGRPTDRLLRFYEERAKGGIGFAVVGIAKIEPHFFGGIAAHSDEFIPDLK
KIADVFHKYDVKCALQLWHPGRYEISFDPNRQPVAPSPIPPPIFTKRTPKELTKEEILQIEEEFADAAVRAKKAGFDAVE
LIGSAGYLISQFFSPATNKRTDEYGGSLENRTRFAVEIIQKIKEKCGESYPVMIRIPGDEFIEGGNTVREMKQIAKILED
AGVVAINVMAGWHESRKPLTTMLVPRGGFAYLAAEIKSAVSVPVIASHRINDPIVAEKILQEGKADMVAMLRALIADPEL
PKKAKEGRFDEIRYCVACNQGCMDMVMQAQPVTCLVNPIVGREAEFENLKAEKPKKVVIVGGGPGGCMAAEMLARKGHKV
VLFEKTDKLGGQLNLAAKSPLGYEFAEVGKYFMNVLPKLGVEVRYNTEADAGKVLAENPDVAVIAVGASPLIPPIPGVEN
AVTAFDVLAGRAEVGNSVVVIGGGGVGCDVAAELANEGKKVTIVEMLPKIGQDIGISTRWTVLMYLKEKGVEMLTNTKAV
EIKPNAVVVEQNGERKELQCDTAVIAVGTKPNNGLYDELQGKVSELYKIGDCVKPRKALDATREGADLALKV

Sequences:

>Translated_632_residues
MKLFEPIEFGGMKVKNRIVMPAAELNYHTPDGRPTDRLLRFYEERAKGGIGFAVVGIAKIEPHFFGGIAAHSDEFIPDLK
KIADVFHKYDVKCALQLWHPGRYEISFDPNRQPVAPSPIPPPIFTKRTPKELTKEEILQIEEEFADAAVRAKKAGFDAVE
LIGSAGYLISQFFSPATNKRTDEYGGSLENRTRFAVEIIQKIKEKCGESYPVMIRIPGDEFIEGGNTVREMKQIAKILED
AGVVAINVMAGWHESRKPLTTMLVPRGGFAYLAAEIKSAVSVPVIASHRINDPIVAEKILQEGKADMVAMLRALIADPEL
PKKAKEGRFDEIRYCVACNQGCMDMVMQAQPVTCLVNPIVGREAEFENLKAEKPKKVVIVGGGPGGCMAAEMLARKGHKV
VLFEKTDKLGGQLNLAAKSPLGYEFAEVGKYFMNVLPKLGVEVRYNTEADAGKVLAENPDVAVIAVGASPLIPPIPGVEN
AVTAFDVLAGRAEVGNSVVVIGGGGVGCDVAAELANEGKKVTIVEMLPKIGQDIGISTRWTVLMYLKEKGVEMLTNTKAV
EIKPNAVVVEQNGERKELQCDTAVIAVGTKPNNGLYDELQGKVSELYKIGDCVKPRKALDATREGADLALKV
>Mature_632_residues
MKLFEPIEFGGMKVKNRIVMPAAELNYHTPDGRPTDRLLRFYEERAKGGIGFAVVGIAKIEPHFFGGIAAHSDEFIPDLK
KIADVFHKYDVKCALQLWHPGRYEISFDPNRQPVAPSPIPPPIFTKRTPKELTKEEILQIEEEFADAAVRAKKAGFDAVE
LIGSAGYLISQFFSPATNKRTDEYGGSLENRTRFAVEIIQKIKEKCGESYPVMIRIPGDEFIEGGNTVREMKQIAKILED
AGVVAINVMAGWHESRKPLTTMLVPRGGFAYLAAEIKSAVSVPVIASHRINDPIVAEKILQEGKADMVAMLRALIADPEL
PKKAKEGRFDEIRYCVACNQGCMDMVMQAQPVTCLVNPIVGREAEFENLKAEKPKKVVIVGGGPGGCMAAEMLARKGHKV
VLFEKTDKLGGQLNLAAKSPLGYEFAEVGKYFMNVLPKLGVEVRYNTEADAGKVLAENPDVAVIAVGASPLIPPIPGVEN
AVTAFDVLAGRAEVGNSVVVIGGGGVGCDVAAELANEGKKVTIVEMLPKIGQDIGISTRWTVLMYLKEKGVEMLTNTKAV
EIKPNAVVVEQNGERKELQCDTAVIAVGTKPNNGLYDELQGKVSELYKIGDCVKPRKALDATREGADLALKV

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=680, Percent_Identity=35.2941176470588, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787939, Length=340, Percent_Identity=32.0588235294118, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17559802, Length=354, Percent_Identity=30.225988700565, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17559804, Length=359, Percent_Identity=28.133704735376, Blast_Score=109, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17540738, Length=347, Percent_Identity=27.0893371757925, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17565138, Length=326, Percent_Identity=27.9141104294479, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=360, Percent_Identity=27.2222222222222, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=324, Percent_Identity=27.7777777777778, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=273, Percent_Identity=27.4725274725275, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325086, Length=222, Percent_Identity=30.1801801801802, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321973, Length=374, Percent_Identity=24.8663101604278, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28574881, Length=185, Percent_Identity=30.2702702702703, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665539, Length=185, Percent_Identity=30.2702702702703, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665547, Length=185, Percent_Identity=30.2702702702703, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665543, Length=185, Percent_Identity=30.2702702702703, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 68707; Mature: 68707

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFEPIEFGGMKVKNRIVMPAAELNYHTPDGRPTDRLLRFYEERAKGGIGFAVVGIAKI
CCCCCCCCCCCEEECCEEEECHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEE
EPHFFGGIAAHSDEFIPDLKKIADVFHKYDVKCALQLWHPGRYEISFDPNRQPVAPSPIP
CCHHHCCEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCCCCCCCC
PPIFTKRTPKELTKEEILQIEEEFADAAVRAKKAGFDAVELIGSAGYLISQFFSPATNKR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCC
TDEYGGSLENRTRFAVEIIQKIKEKCGESYPVMIRIPGDEFIEGGNTVREMKQIAKILED
CHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHC
AGVVAINVMAGWHESRKPLTTMLVPRGGFAYLAAEIKSAVSVPVIASHRINDPIVAEKIL
CCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHH
QEGKADMVAMLRALIADPELPKKAKEGRFDEIRYCVACNQGCMDMVMQAQPVTCLVNPIV
HCCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHHEEECCCCCHHHHHHCCCCCEEEECCCC
GREAEFENLKAEKPKKVVIVGGGPGGCMAAEMLARKGHKVVLFEKTDKLGGQLNLAAKSP
CCCCCHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCC
LGYEFAEVGKYFMNVLPKLGVEVRYNTEADAGKVLAENPDVAVIAVGASPLIPPIPGVEN
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCH
AVTAFDVLAGRAEVGNSVVVIGGGGVGCDVAAELANEGKKVTIVEMLPKIGQDIGISTRW
HHHHHHHHHCHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCHH
TVLMYLKEKGVEMLTNTKAVEIKPNAVVVEQNGERKELQCDTAVIAVGTKPNNGLYDELQ
EEEEEEHHCCHHHHCCCEEEEECCCEEEECCCCCCCEEEECEEEEEEECCCCCCCHHHHH
GKVSELYKIGDCVKPRKALDATREGADLALKV
HHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEC
>Mature Secondary Structure
MKLFEPIEFGGMKVKNRIVMPAAELNYHTPDGRPTDRLLRFYEERAKGGIGFAVVGIAKI
CCCCCCCCCCCEEECCEEEECHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEE
EPHFFGGIAAHSDEFIPDLKKIADVFHKYDVKCALQLWHPGRYEISFDPNRQPVAPSPIP
CCHHHCCEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCCCCCCCC
PPIFTKRTPKELTKEEILQIEEEFADAAVRAKKAGFDAVELIGSAGYLISQFFSPATNKR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCC
TDEYGGSLENRTRFAVEIIQKIKEKCGESYPVMIRIPGDEFIEGGNTVREMKQIAKILED
CHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCHHHHHHHHHHHHHHC
AGVVAINVMAGWHESRKPLTTMLVPRGGFAYLAAEIKSAVSVPVIASHRINDPIVAEKIL
CCEEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHH
QEGKADMVAMLRALIADPELPKKAKEGRFDEIRYCVACNQGCMDMVMQAQPVTCLVNPIV
HCCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHHEEECCCCCHHHHHHCCCCCEEEECCCC
GREAEFENLKAEKPKKVVIVGGGPGGCMAAEMLARKGHKVVLFEKTDKLGGQLNLAAKSP
CCCCCHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCC
LGYEFAEVGKYFMNVLPKLGVEVRYNTEADAGKVLAENPDVAVIAVGASPLIPPIPGVEN
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCH
AVTAFDVLAGRAEVGNSVVVIGGGGVGCDVAAELANEGKKVTIVEMLPKIGQDIGISTRW
HHHHHHHHHCHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCHH
TVLMYLKEKGVEMLTNTKAVEIKPNAVVVEQNGERKELQCDTAVIAVGTKPNNGLYDELQ
EEEEEEHHCCHHHHCCCEEEEECCCEEEECCCCCCCEEEECEEEEEEECCCCCCCHHHHH
GKVSELYKIGDCVKPRKALDATREGADLALKV
HHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]