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Identifier: 11497746

GI number: 11497746

Start: 117660

End: 118325

Strand: Reverse

Name: Not Available

Synonym: AF0129

Alternate gene names: 11497746

Gene position: 118325-117660 (Counterclockwise)

Preceding gene: 11497747

Following gene: 11497744

Centisome position: 5.43

GC content: 49.25

Gene sequence:

>666_bases
GTGCTTCAAATGAATCTCGAAGAGCTGAGGAGGATACAGGAGGAGATGTCCAGAAGTGTGGTTCTCGAAGACTTAATCCC
TCTTGAAGAGCTTGAGTACGTTGTGGGTGTTGATCAGGCCTTTATCAGCGATGAGGTTGTCTCATGTGCGGTCAAGCTGA
CCTTTCCGGAACTGGAGGTTGTTGATAAAGCTGTGAGGGTTGAGAAGGTCACTTTCCCCTACATCCCCACCTTTCTCATG
TTCAGGGAGGGAGAGCCTGCAGTTAATGCGGTCAAAGGGCTTGTGGATGACAGAGCGGCAATCATGGTTGATGGGAGCGG
AATTGCCCATCCGAGAAGGTGCGGGCTTGCAACATACATCGCCCTAAAGCTGAGAAAGCCGACTGTGGGGATAACAAAGA
AAAGGCTTTTTGGTGAGATGGTAGAGGTGGAAGATGGGCTTTGGAGGCTTTTAGATGGAAGTGAAACCATAGGCTACGCC
CTTAAAAGCTGCAGGAGGTGCAAACCAATCTTCATCTCACCGGGGAGTTACATATCTCCTGACTCAGCCTTGGAGCTGAC
GAGAAAGTGCCTTAAAGGCTACAAGCTTCCTGAGCCGATAAGAATCGCCGACAAACTTACCAAGGAGGTTAAGAGGGAGT
TGACTCCAACCTCAAAGCTTAAATAA

Upstream 100 bases:

>100_bases
ACTACCTCCCCGAGCTGCTCTGGCGGAAGAGGGATTCGGCATTGGTGAAGCTGAAGCACAACATAGAGGATGTGAAGAGG
GTTCACTCAAAATACTGGAA

Downstream 100 bases:

>100_bases
CAGTTTGTTCAAAAGTGTTCTGAAGCGGGGTGGGGTAGTCAGGTGATCCCGGCGGGCTCATAACCCGCAGATCCGTGGTT
CAAATCCACGCCCCGCTATT

Product: hypothetical protein

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MLQMNLEELRRIQEEMSRSVVLEDLIPLEELEYVVGVDQAFISDEVVSCAVKLTFPELEVVDKAVRVEKVTFPYIPTFLM
FREGEPAVNAVKGLVDDRAAIMVDGSGIAHPRRCGLATYIALKLRKPTVGITKKRLFGEMVEVEDGLWRLLDGSETIGYA
LKSCRRCKPIFISPGSYISPDSALELTRKCLKGYKLPEPIRIADKLTKEVKRELTPTSKLK

Sequences:

>Translated_221_residues
MLQMNLEELRRIQEEMSRSVVLEDLIPLEELEYVVGVDQAFISDEVVSCAVKLTFPELEVVDKAVRVEKVTFPYIPTFLM
FREGEPAVNAVKGLVDDRAAIMVDGSGIAHPRRCGLATYIALKLRKPTVGITKKRLFGEMVEVEDGLWRLLDGSETIGYA
LKSCRRCKPIFISPGSYISPDSALELTRKCLKGYKLPEPIRIADKLTKEVKRELTPTSKLK
>Mature_221_residues
MLQMNLEELRRIQEEMSRSVVLEDLIPLEELEYVVGVDQAFISDEVVSCAVKLTFPELEVVDKAVRVEKVTFPYIPTFLM
FREGEPAVNAVKGLVDDRAAIMVDGSGIAHPRRCGLATYIALKLRKPTVGITKKRLFGEMVEVEDGLWRLLDGSETIGYA
LKSCRRCKPIFISPGSYISPDSALELTRKCLKGYKLPEPIRIADKLTKEVKRELTPTSKLK

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=206, Percent_Identity=34.4660194174757, Blast_Score=95, Evalue=5e-20,
Organism=Escherichia coli, GI87082357, Length=211, Percent_Identity=40.7582938388626, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_ARCFU (O30108)

Other databases:

- EMBL:   AE000782
- PIR:   A69266
- RefSeq:   NP_068968.1
- ProteinModelPortal:   O30108
- GeneID:   1483340
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0129
- NMPDR:   fig|224325.1.peg.127
- TIGR:   AF_0129
- HOGENOM:   HBG716360
- OMA:   CMRGYRL
- ProtClustDB:   CLSK2746315
- BioCyc:   AFUL224325:AF_0129-MONOMER
- BRENDA:   3.1.21.7
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 24873; Mature: 24873

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQMNLEELRRIQEEMSRSVVLEDLIPLEELEYVVGVDQAFISDEVVSCAVKLTFPELEV
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHEECCCHHHH
VDKAVRVEKVTFPYIPTFLMFREGEPAVNAVKGLVDDRAAIMVDGSGIAHPRRCGLATYI
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHH
ALKLRKPTVGITKKRLFGEMVEVEDGLWRLLDGSETIGYALKSCRRCKPIFISPGSYISP
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCC
DSALELTRKCLKGYKLPEPIRIADKLTKEVKRELTPTSKLK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLQMNLEELRRIQEEMSRSVVLEDLIPLEELEYVVGVDQAFISDEVVSCAVKLTFPELEV
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHEECCCHHHH
VDKAVRVEKVTFPYIPTFLMFREGEPAVNAVKGLVDDRAAIMVDGSGIAHPRRCGLATYI
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHH
ALKLRKPTVGITKKRLFGEMVEVEDGLWRLLDGSETIGYALKSCRRCKPIFISPGSYISP
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCC
DSALELTRKCLKGYKLPEPIRIADKLTKEVKRELTPTSKLK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475; 11056288