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The map label for this gene is act-1

Identifier: 11497737

GI number: 11497737

Start: 107875

End: 108837

Strand: Reverse

Name: act-1

Synonym: AF0117

Alternate gene names: 11497737

Gene position: 108837-107875 (Counterclockwise)

Preceding gene: 11497738

Following gene: 11497733

Centisome position: 5.0

GC content: 50.67

Gene sequence:

>963_bases
ATGAGGAGAGGAGGAACAGCTTTTTCGTCTAAGTGTAAGATCTGCGGTAAAGCCGCTCCTTTCTCTCTTCCAGTCTGCCC
TGAGTGCTCCAAGAAAGATGCAGCTCTCGAAATCGCCACCTCACTTCACCCACCTCTATACGAGAAAAGAGTCGAGGGCT
GCAACCTCTGCAGCAACAAATGCTCTGGTATTGCCCTTTGCGGCAAACCTGAATACGGCAAATTGACCTACTACGAAGAC
CCTTTGCCGACGAACTGCTGCAACGCCTGGTTTTGCAATGGGAGCAAGCTAACAGGGACGAATCTCGCCGTGTTTTACTA
CGGATGCAACTTCGACTGTCTTTACTGCCAGAACTGGACCCACAAATACTTCGAAGACGTCAGAACCGTTAGTGTTGACG
AGCTTCTGCGTGCTGCGAAAAACTTCAGAGTCAGGTGCGTCTGCCATTTTGGGGGCTCACCCGAGCCGCAGCTCCCATTC
GCTTTGAAGTTCAGCAGAAAAGCACTGGAATTCAAAGAAGACCTGATGATTTGCTGGGAGTGGAATGGGGCCGGAAACAC
CTCTCTGGCGTTAAAGGCCGCCGAATTAAGCTCAAGGTCTGGAGGAACTGTGAAGTTCGACCTCAAGGCGTGGAATCCAA
ATCTGCACGTTTTGCTTACGGGGAGAGATAATGAGAGGGTAAAGGACAACTTCGAGAGGATTTACGACAAACACGGAGAG
GTGCTATCCGCAACAACACTGCTGGTGCCCTACTACGTTGATGAGGAGGAGGTGGAGGGTATTGCCCGGTTCATTTCCTC
GTTCAGCACTGAAATTCCCTACAGCCTCCTCGTCTTCCATCCGGACTACAGAATGTCCGACTTCCCCGTCACCCCTGTGC
AGCAGGTGAAGAGATGCTACAAAGCGGCCAAAAAACACCTTAAAAACGTGAACATCGGGAATCTACATCTTCTTGGACTT
TAA

Upstream 100 bases:

>100_bases
TCAGAAGCGATATCCACCTACAGCCTCTTCTGCGGTGATTTGAAGAAGCTGAGGATGGTTGCGGAGAACATGCTCTCGAT
GATTCTCGCCGCTGGGAGGT

Downstream 100 bases:

>100_bases
AAGCTCTATCACCCCATCAAATGTGTACTCTTCCGGTATCTCCGCCCTTATACCCTTTTCAGCCAAAACCTTTGCCGTGG
GAGGGCCGATGGCTACAACG

Product: pyruvate formate-lyase activating enzyme (act-1)

Products: NA

Alternate protein names: Radical SAM Domain-Containing Protein; Pyruvate Formate-Lyase Activating; Pyruvate-Formate Lyase-Activating; Radical Sam Domain Protein; Pyruvate-Formate Lyase-Activating PflA; [Pyruvate Formate-Lyase]-Activating; Pyruvate Formate-Lyase Activating -Like Protein; Radical SAM Family Protein; Radical SAM

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRRGGTAFSSKCKICGKAAPFSLPVCPECSKKDAALEIATSLHPPLYEKRVEGCNLCSNKCSGIALCGKPEYGKLTYYED
PLPTNCCNAWFCNGSKLTGTNLAVFYYGCNFDCLYCQNWTHKYFEDVRTVSVDELLRAAKNFRVRCVCHFGGSPEPQLPF
ALKFSRKALEFKEDLMICWEWNGAGNTSLALKAAELSSRSGGTVKFDLKAWNPNLHVLLTGRDNERVKDNFERIYDKHGE
VLSATTLLVPYYVDEEEVEGIARFISSFSTEIPYSLLVFHPDYRMSDFPVTPVQQVKRCYKAAKKHLKNVNIGNLHLLGL

Sequences:

>Translated_320_residues
MRRGGTAFSSKCKICGKAAPFSLPVCPECSKKDAALEIATSLHPPLYEKRVEGCNLCSNKCSGIALCGKPEYGKLTYYED
PLPTNCCNAWFCNGSKLTGTNLAVFYYGCNFDCLYCQNWTHKYFEDVRTVSVDELLRAAKNFRVRCVCHFGGSPEPQLPF
ALKFSRKALEFKEDLMICWEWNGAGNTSLALKAAELSSRSGGTVKFDLKAWNPNLHVLLTGRDNERVKDNFERIYDKHGE
VLSATTLLVPYYVDEEEVEGIARFISSFSTEIPYSLLVFHPDYRMSDFPVTPVQQVKRCYKAAKKHLKNVNIGNLHLLGL
>Mature_320_residues
MRRGGTAFSSKCKICGKAAPFSLPVCPECSKKDAALEIATSLHPPLYEKRVEGCNLCSNKCSGIALCGKPEYGKLTYYED
PLPTNCCNAWFCNGSKLTGTNLAVFYYGCNFDCLYCQNWTHKYFEDVRTVSVDELLRAAKNFRVRCVCHFGGSPEPQLPF
ALKFSRKALEFKEDLMICWEWNGAGNTSLALKAAELSSRSGGTVKFDLKAWNPNLHVLLTGRDNERVKDNFERIYDKHGE
VLSATTLLVPYYVDEEEVEGIARFISSFSTEIPYSLLVFHPDYRMSDFPVTPVQQVKRCYKAAKKHLKNVNIGNLHLLGL

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36011; Mature: 36011

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRGGTAFSSKCKICGKAAPFSLPVCPECSKKDAALEIATSLHPPLYEKRVEGCNLCSNK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCHHHHHHHCCCHHHCCC
CSGIALCGKPEYGKLTYYEDPLPTNCCNAWFCNGSKLTGTNLAVFYYGCNFDCLYCQNWT
CCCEEEECCCCCCCEEEECCCCCCCCCCCEEECCCEECCCEEEEEEEECCEEEEEECCCC
HKYFEDVRTVSVDELLRAAKNFRVRCVCHFGGSPEPQLPFALKFSRKALEFKEDLMICWE
HHHHHHHHCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEHHHHHHCCCCCEEEEEE
WNGAGNTSLALKAAELSSRSGGTVKFDLKAWNPNLHVLLTGRDNERVKDNFERIYDKHGE
ECCCCCCEEEEEEEHHCCCCCCEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHCCC
VLSATTLLVPYYVDEEEVEGIARFISSFSTEIPYSLLVFHPDYRMSDFPVTPVQQVKRCY
EEEEEEEEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
KAAKKHLKNVNIGNLHLLGL
HHHHHHHCCCCCCCEEEEEC
>Mature Secondary Structure
MRRGGTAFSSKCKICGKAAPFSLPVCPECSKKDAALEIATSLHPPLYEKRVEGCNLCSNK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCHHHHHHHCCCHHHCCC
CSGIALCGKPEYGKLTYYEDPLPTNCCNAWFCNGSKLTGTNLAVFYYGCNFDCLYCQNWT
CCCEEEECCCCCCCEEEECCCCCCCCCCCEEECCCEECCCEEEEEEEECCEEEEEECCCC
HKYFEDVRTVSVDELLRAAKNFRVRCVCHFGGSPEPQLPFALKFSRKALEFKEDLMICWE
HHHHHHHHCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEHHHHHHCCCCCEEEEEE
WNGAGNTSLALKAAELSSRSGGTVKFDLKAWNPNLHVLLTGRDNERVKDNFERIYDKHGE
ECCCCCCEEEEEEEHHCCCCCCEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHCCC
VLSATTLLVPYYVDEEEVEGIARFISSFSTEIPYSLLVFHPDYRMSDFPVTPVQQVKRCY
EEEEEEEEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
KAAKKHLKNVNIGNLHLLGL
HHHHHHHCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA