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The map label for this gene is purP

Identifier: 11497733

GI number: 11497733

Start: 105046

End: 106125

Strand: Reverse

Name: purP

Synonym: AF0113

Alternate gene names: 11497733

Gene position: 106125-105046 (Counterclockwise)

Preceding gene: 11497737

Following gene: 11497732

Centisome position: 4.87

GC content: 48.33

Gene sequence:

>1080_bases
GTGGGGGCGATGAGGGATAAGATACTCTCAGTCCTCGAAGATTATGATAAGGCAAACATCCACATAGGGACGATTGGAAG
CCATTCGGCACTCAACATCCTCAAAGGGGCAAAGGATGAGGGGTTCAGCACGGTCTGCATTTGCAGGGAGAGGGAGAAAA
AGGTTTACGAGAGCTTCGGGGTTGCTGATAAGTTCGTAATCGTTTCAGACTATTCAGATTTACTTGATGAAAACATTCAG
GAAGAGCTTAGGCACTTGAACACAATTTTAATCCCTCACGGCTCCTTCAACGCCTACATCGGTAAATTCGATTCGTTGCT
GCTACCGATGTTCGGAAACAGAGAGCTGATGATTTGGGAAACGGACAGGGACAGGCAGAGAGAGTGGCTGGAAAGGTCAG
GAGTCAGAATGCCGAAGAAATACAGCAGGCCAGAGGAGATAGAGGGGCTGGCCATTGTAAAGTATCCGGGGGCGAAAGGG
GGAAAGGGTTACTTCATAGTGTCCTCCCCAGAGGAATTTTATGAAATTTCGGATGAAATGGTGAAAAAAGGGCTTATTGG
TGAGGAGGACATCAAAAACGCTGAAATACAGGAGTACATTCTCGGTGCGAACATTTACTTCTCCTTCTTCTACTCCCCGG
TTTACGGAAGGGTTGAGCTAATAGCGGTGGACAGAAGGTATGAATCAACTGCGGATGCTTTGGGCAAGGTTCCGGCGAAG
GAGCAGATTAGAGCTAACATAAACCCAACATACACCGTTATCGGCAACTTTCCGGTGGTTCTACGAGAGTCTCTGCTCGT
GCAGGCATTCGAGGCAGCTGAGGGCATCGTTGAGGTATCCAAGGAGATAGCCTACCCCGGCATGGTCGGAGCATTCTGCA
TTGAGTCAATTTTTGATGAGAACGCACTGATGTACGTCTTTGAGATTTCTGCAAGAATTGTGGCAGGGACGAATGTCGGA
ATTCCGACCTCTCCATACTCCTACATTCTCTTTGGAGAGAACATGTACATGGGGAGGAGAATAGCGAGGGAGATAAAGCT
TGCAGCGGAGAAGGACATGCTCGGCGAGTTGATTTACTGA

Upstream 100 bases:

>100_bases
TCTCGATGCTATCGCTGTACTTTTCCCAGCTTGACATCCTTTTAAACTTGTTGAGCTAAGATTTTAACTTTTCGAATGGT
TTATATAACCTTCACGAACG

Downstream 100 bases:

>100_bases
ATTTCTTTCCAGAGCGAAAAGTATTAAATTTAACGACTTATTAGGGAAAATATGGAGGATGAGATAAGGAAAGCGGCAGA
GATTCTTGCCAAATCGAAGC

Product: 5-formaminoimidazole-4-carboxamide-1-(beta)-D- ribofuranosyl 5'-monophosphate synthetase

Products: NA

Alternate protein names: 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MGAMRDKILSVLEDYDKANIHIGTIGSHSALNILKGAKDEGFSTVCICREREKKVYESFGVADKFVIVSDYSDLLDENIQ
EELRHLNTILIPHGSFNAYIGKFDSLLLPMFGNRELMIWETDRDRQREWLERSGVRMPKKYSRPEEIEGLAIVKYPGAKG
GKGYFIVSSPEEFYEISDEMVKKGLIGEEDIKNAEIQEYILGANIYFSFFYSPVYGRVELIAVDRRYESTADALGKVPAK
EQIRANINPTYTVIGNFPVVLRESLLVQAFEAAEGIVEVSKEIAYPGMVGAFCIESIFDENALMYVFEISARIVAGTNVG
IPTSPYSYILFGENMYMGRRIAREIKLAAEKDMLGELIY

Sequences:

>Translated_359_residues
MGAMRDKILSVLEDYDKANIHIGTIGSHSALNILKGAKDEGFSTVCICREREKKVYESFGVADKFVIVSDYSDLLDENIQ
EELRHLNTILIPHGSFNAYIGKFDSLLLPMFGNRELMIWETDRDRQREWLERSGVRMPKKYSRPEEIEGLAIVKYPGAKG
GKGYFIVSSPEEFYEISDEMVKKGLIGEEDIKNAEIQEYILGANIYFSFFYSPVYGRVELIAVDRRYESTADALGKVPAK
EQIRANINPTYTVIGNFPVVLRESLLVQAFEAAEGIVEVSKEIAYPGMVGAFCIESIFDENALMYVFEISARIVAGTNVG
IPTSPYSYILFGENMYMGRRIAREIKLAAEKDMLGELIY
>Mature_358_residues
GAMRDKILSVLEDYDKANIHIGTIGSHSALNILKGAKDEGFSTVCICREREKKVYESFGVADKFVIVSDYSDLLDENIQE
ELRHLNTILIPHGSFNAYIGKFDSLLLPMFGNRELMIWETDRDRQREWLERSGVRMPKKYSRPEEIEGLAIVKYPGAKGG
KGYFIVSSPEEFYEISDEMVKKGLIGEEDIKNAEIQEYILGANIYFSFFYSPVYGRVELIAVDRRYESTADALGKVPAKE
QIRANINPTYTVIGNFPVVLRESLLVQAFEAAEGIVEVSKEIAYPGMVGAFCIESIFDENALMYVFEISARIVAGTNVGI
PTSPYSYILFGENMYMGRRIAREIKLAAEKDMLGELIY

Specific function: Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'- monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1- beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates

COG id: COG1759

COG function: function code R; ATP-utilizing enzymes of ATP-grasp superfamily (probably carboligases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURP_ARCFU (O30123)

Other databases:

- EMBL:   AE000782
- PIR:   A69264
- RefSeq:   NP_068954.1
- ProteinModelPortal:   O30123
- SMR:   O30123
- GeneID:   1483325
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0113
- NMPDR:   fig|224325.1.peg.113
- TIGR:   AF_0113
- HOGENOM:   HBG392202
- OMA:   VFEISAR
- ProtClustDB:   PRK13278
- BioCyc:   AFUL224325:AF_0113-MONOMER
- HAMAP:   MF_01163
- InterPro:   IPR013816
- InterPro:   IPR009720
- InterPro:   IPR010672
- InterPro:   IPR013817
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20

Pfam domain/function: PF06849 DUF1246; PF06973 DUF1297

EC number: NA

Molecular weight: Translated: 40560; Mature: 40429

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 28-28 BINDING 95-95 BINDING 228-228 BINDING 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAMRDKILSVLEDYDKANIHIGTIGSHSALNILKGAKDEGFSTVCICREREKKVYESFG
CCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHC
VADKFVIVSDYSDLLDENIQEELRHLNTILIPHGSFNAYIGKFDSLLLPMFGNRELMIWE
CCCEEEEEECHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEE
TDRDRQREWLERSGVRMPKKYSRPEEIEGLAIVKYPGAKGGKGYFIVSSPEEFYEISDEM
CCCHHHHHHHHHCCCCCCHHCCCCCCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHH
VKKGLIGEEDIKNAEIQEYILGANIYFSFFYSPVYGRVELIAVDRRYESTADALGKVPAK
HHHCCCCCHHCCCCHHHHHHHCCHHEEEEHHCCCCCCEEEEEECCCHHHHHHHHHCCCCH
EQIRANINPTYTVIGNFPVVLRESLLVQAFEAAEGIVEVSKEIAYPGMVGAFCIESIFDE
HHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
NALMYVFEISARIVAGTNVGIPTSPYSYILFGENMYMGRRIAREIKLAAEKDMLGELIY
CCEEEEEEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GAMRDKILSVLEDYDKANIHIGTIGSHSALNILKGAKDEGFSTVCICREREKKVYESFG
CHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHC
VADKFVIVSDYSDLLDENIQEELRHLNTILIPHGSFNAYIGKFDSLLLPMFGNRELMIWE
CCCEEEEEECHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEE
TDRDRQREWLERSGVRMPKKYSRPEEIEGLAIVKYPGAKGGKGYFIVSSPEEFYEISDEM
CCCHHHHHHHHHCCCCCCHHCCCCCCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHH
VKKGLIGEEDIKNAEIQEYILGANIYFSFFYSPVYGRVELIAVDRRYESTADALGKVPAK
HHHCCCCCHHCCCCHHHHHHHCCHHEEEEHHCCCCCCEEEEEECCCHHHHHHHHHCCCCH
EQIRANINPTYTVIGNFPVVLRESLLVQAFEAAEGIVEVSKEIAYPGMVGAFCIESIFDE
HHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
NALMYVFEISARIVAGTNVGIPTSPYSYILFGENMYMGRRIAREIKLAAEKDMLGELIY
CCEEEEEEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475