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The map label for this gene is mazG [H]

Identifier: 114800510

GI number: 114800510

Start: 2079003

End: 2079830

Strand: Direct

Name: mazG [H]

Synonym: HNE_2004

Alternate gene names: 114800510

Gene position: 2079003-2079830 (Clockwise)

Preceding gene: 114797178

Following gene: 114798198

Centisome position: 56.11

GC content: 65.34

Gene sequence:

>828_bases
ATGTCCTCACCCGATTCCAAGCCTGCTGGCGCCGCAGAGGCGCTGCTGACACTGATCGACATCATGGCTCGGCTGAGAAC
GCCGCAGACAGGCTGCCCGTGGGATCTGGAGCAGACCTTCGCGACGATAGCCCCCTACACGATCGAGGAATCCTATGAGG
TCGCCGATGCAATTGAGCGCGGGGATATGGGCGACCTGCGCGAGGAACTGGGCGATCTGCTGTTCCAGGTGATCTTTCAC
AGCCGGATGGCCGAAGAAGCTGGCGCATTTGCGCTGGCGGATGTTGTCCACGCCATCAACACCAAGATGATTCGCCGCCA
TCCGCATGTGTTCGAGGCCGCAGACGGGCGAGACGCCGATGGGCAGACCGTGGCGTGGGAAGAGATGAAAGCGGCCGAGC
GGGCCGCGAAGGGGAAGACGGCGGCCGCCCCCAGCGCGCTGGACGGCGTGGCGCTCTCCCTGCCTGCCCTGCTGCGCGCC
GAGAAGCTTCAGAAGCGGGCCGCGCGGACAGGCTTTGACTGGACCGAGGCGGCGCACATCTTCGACAAGCTGGAAGAAGA
GACCGCTGAGGTGAAGGAAGCTATCGCGAGCGGAGACGCTGACGCCATTGAGGACGAGATTGGCGACCTGCTGTTCGTGG
CGGCCAACCTTGCGCGGCGGCTGTCGGTTGATCCTGAGCAGGCACTGCGGCGGGCGAACGGCAAGTTTGAGCGGCGGTTC
CGGGCGATGGAGACAGCGGCGGCGGACGCGGGCGTGGCGTTTGCGTCGCTCAGTCTGGATGAGCAGGAAGCGTACTGGCA
GGCGGTGAAGAAGGCGGAGCGGGGCTAA

Upstream 100 bases:

>100_bases
AGCGTTTGATCGGGCGCAAGCTGCAAGATGACCTATTGCTCTTGCTGCACCGGCGCGGGAGCCTAAACACGGAGGCCAAG
ATCAAGGCGGAGCGTAGCGC

Downstream 100 bases:

>100_bases
AACCAAGCCCTCTTCAGAGTATCGACACACCCGCCGCCTGCTCCGGGAATTCGCCGCGGAATTGGCCGAGGCGTTCGGTG
GAGAGGCGCAGGGTGAGGTC

Product: MazG family protein

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSSPDSKPAGAAEALLTLIDIMARLRTPQTGCPWDLEQTFATIAPYTIEESYEVADAIERGDMGDLREELGDLLFQVIFH
SRMAEEAGAFALADVVHAINTKMIRRHPHVFEAADGRDADGQTVAWEEMKAAERAAKGKTAAAPSALDGVALSLPALLRA
EKLQKRAARTGFDWTEAAHIFDKLEEETAEVKEAIASGDADAIEDEIGDLLFVAANLARRLSVDPEQALRRANGKFERRF
RAMETAAADAGVAFASLSLDEQEAYWQAVKKAERG

Sequences:

>Translated_275_residues
MSSPDSKPAGAAEALLTLIDIMARLRTPQTGCPWDLEQTFATIAPYTIEESYEVADAIERGDMGDLREELGDLLFQVIFH
SRMAEEAGAFALADVVHAINTKMIRRHPHVFEAADGRDADGQTVAWEEMKAAERAAKGKTAAAPSALDGVALSLPALLRA
EKLQKRAARTGFDWTEAAHIFDKLEEETAEVKEAIASGDADAIEDEIGDLLFVAANLARRLSVDPEQALRRANGKFERRF
RAMETAAADAGVAFASLSLDEQEAYWQAVKKAERG
>Mature_274_residues
SSPDSKPAGAAEALLTLIDIMARLRTPQTGCPWDLEQTFATIAPYTIEESYEVADAIERGDMGDLREELGDLLFQVIFHS
RMAEEAGAFALADVVHAINTKMIRRHPHVFEAADGRDADGQTVAWEEMKAAERAAKGKTAAAPSALDGVALSLPALLRAE
KLQKRAARTGFDWTEAAHIFDKLEEETAEVKEAIASGDADAIEDEIGDLLFVAANLARRLSVDPEQALRRANGKFERRFR
AMETAAADAGVAFASLSLDEQEAYWQAVKKAERG

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=260, Percent_Identity=51.1538461538461, Blast_Score=256, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 29930; Mature: 29799

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPDSKPAGAAEALLTLIDIMARLRTPQTGCPWDLEQTFATIAPYTIEESYEVADAIER
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHC
GDMGDLREELGDLLFQVIFHSRMAEEAGAFALADVVHAINTKMIRRHPHVFEAADGRDAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCC
GQTVAWEEMKAAERAAKGKTAAAPSALDGVALSLPALLRAEKLQKRAARTGFDWTEAAHI
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FDKLEEETAEVKEAIASGDADAIEDEIGDLLFVAANLARRLSVDPEQALRRANGKFERRF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHH
RAMETAAADAGVAFASLSLDEQEAYWQAVKKAERG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSPDSKPAGAAEALLTLIDIMARLRTPQTGCPWDLEQTFATIAPYTIEESYEVADAIER
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHC
GDMGDLREELGDLLFQVIFHSRMAEEAGAFALADVVHAINTKMIRRHPHVFEAADGRDAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCC
GQTVAWEEMKAAERAAKGKTAAAPSALDGVALSLPALLRAEKLQKRAARTGFDWTEAAHI
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
FDKLEEETAEVKEAIASGDADAIEDEIGDLLFVAANLARRLSVDPEQALRRANGKFERRF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHH
RAMETAAADAGVAFASLSLDEQEAYWQAVKKAERG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]