Click here to switch to the map view.

The map label for this gene is ispDF

Identifier: 114798198

GI number: 114798198

Start: 2091646

End: 2092782

Strand: Direct

Name: ispDF

Synonym: HNE_2014

Alternate gene names: 114798198

Gene position: 2091646-2092782 (Clockwise)

Preceding gene: 114800510

Following gene: 114797375

Centisome position: 56.45

GC content: 65.0

Gene sequence:

>1137_bases
ATGACCGAGACCGTCGCCATAATCGTTGCCGGAGGCCGGGGCCAGCGCGCAGGCGCAGAGCGCCCGAAACAGTGGCAAAT
GCTGCTGGGAAAGCGGGTGATCGACTGGTCAATTGCCGCATTTGTGGACCATCCGCAGATCTCGCAAGTGGTGATCGTGG
CGGGGGATGAGCTGGGTGACTGTTCCGCGGAGCCCAAAATTATTCAAGCAAAACCGGGCAATACGCGCACCCAATCTGTG
CTGAGCGGGCTGGCGGCGGCGACTATTTCGGAAGACGCCACTGTCGTTATACATGATGCTGCGCGGCCGGGTATTGATGC
GGCAACTATCTCCAGCCTGATCGCGCGCCTGCAGGACCCATCTGTGTCAGGCGCGGCCCCCGCGATGCCAGTTGCGGACG
CTTTGAAGACAAACTCGGGACAAAGCTGGACAAATGTGGACAGGACCGGCCTCGTCCGGGTCCAGACACCGCAGGCCTTC
CGCCTCGGCGAGATCCGCGCGGCGCTTTCCGCGGCCGGTCCCGATCTGGTCGATGACCTGACCGCCATCGAGGCTGCCGG
CGGGAGGGTCGAGATCGTCTCCGGCTCGGCGCGGCTGACCAAGATCACTTACCCAGAGGATTTCGACATGCTGGCCCGCC
TCCTCTCCCCCACCGGCGCCCCGCGCATCGGCAAGGGCTATGACGTGCATGAGTTTGAGGCGGGGGATCATGTCACCTTG
TGCGGGGTGGCCATTCCCCACATCGCCAAACTCAAGGGCCATTCGGACGCGGACGCGGCCTGGCATGCGCTGACCGATGC
GATCCTTGGCGCGGTGGCGCTGGGGGATATTGGGGATCACTTTCCGCCATCGGACCCGCAGTGGAAGGGGGCTGACAGTG
GCCTCTTTCTCAAAGAGGCCCAGAGGCTCGCCGAGGCGAAGGGCTATGTCATCGCCAATTGCGACATCACGGTGATCTGT
GAAGCGCCCAAGGTGAAGCCCCACCGCGAGGCGATGCGGGCGCGGACGGCGGAGCTGCTCGGGCTGCCGCTGGACGCTGT
GAGCGTGAAGGCGACGACGACGGAGGGGCTGGGCTTTACCGGGCGGCGCGAGGGGATTGCGGCGGAGGCTGTGGCGCTGC
TGATGCCGAAGGGGTGA

Upstream 100 bases:

>100_bases
TTCATTTTTTCGCAAGCGCACAAATCATGGGCATTTCTTTCCCTTTTCGGGACATTTCGGAGAGTAGCCAGCCGCGTGCG
GGTCGCCTACAAGCCAGATC

Downstream 100 bases:

>100_bases
GGCAACGGAGCGAGCGAAGCCCTCTTGGGTATCGTCACTCCCGATGGCCGCAGGCCATCTAGGAGCCTAGCTCCTGCCAT
TGCCGACTGATCTGACGGAA

Product: IspD/IspF bifunctional enzyme

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTETVAIIVAGGRGQRAGAERPKQWQMLLGKRVIDWSIAAFVDHPQISQVVIVAGDELGDCSAEPKIIQAKPGNTRTQSV
LSGLAAATISEDATVVIHDAARPGIDAATISSLIARLQDPSVSGAAPAMPVADALKTNSGQSWTNVDRTGLVRVQTPQAF
RLGEIRAALSAAGPDLVDDLTAIEAAGGRVEIVSGSARLTKITYPEDFDMLARLLSPTGAPRIGKGYDVHEFEAGDHVTL
CGVAIPHIAKLKGHSDADAAWHALTDAILGAVALGDIGDHFPPSDPQWKGADSGLFLKEAQRLAEAKGYVIANCDITVIC
EAPKVKPHREAMRARTAELLGLPLDAVSVKATTTEGLGFTGRREGIAAEAVALLMPKG

Sequences:

>Translated_378_residues
MTETVAIIVAGGRGQRAGAERPKQWQMLLGKRVIDWSIAAFVDHPQISQVVIVAGDELGDCSAEPKIIQAKPGNTRTQSV
LSGLAAATISEDATVVIHDAARPGIDAATISSLIARLQDPSVSGAAPAMPVADALKTNSGQSWTNVDRTGLVRVQTPQAF
RLGEIRAALSAAGPDLVDDLTAIEAAGGRVEIVSGSARLTKITYPEDFDMLARLLSPTGAPRIGKGYDVHEFEAGDHVTL
CGVAIPHIAKLKGHSDADAAWHALTDAILGAVALGDIGDHFPPSDPQWKGADSGLFLKEAQRLAEAKGYVIANCDITVIC
EAPKVKPHREAMRARTAELLGLPLDAVSVKATTTEGLGFTGRREGIAAEAVALLMPKG
>Mature_377_residues
TETVAIIVAGGRGQRAGAERPKQWQMLLGKRVIDWSIAAFVDHPQISQVVIVAGDELGDCSAEPKIIQAKPGNTRTQSVL
SGLAAATISEDATVVIHDAARPGIDAATISSLIARLQDPSVSGAAPAMPVADALKTNSGQSWTNVDRTGLVRVQTPQAFR
LGEIRAALSAAGPDLVDDLTAIEAAGGRVEIVSGSARLTKITYPEDFDMLARLLSPTGAPRIGKGYDVHEFEAGDHVTLC
GVAIPHIAKLKGHSDADAAWHALTDAILGAVALGDIGDHFPPSDPQWKGADSGLFLKEAQRLAEAKGYVIANCDITVICE
APKVKPHREAMRARTAELLGLPLDAVSVKATTTEGLGFTGRREGIAAEAVALLMPKG

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=58.4415584415584, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=31.8777292576419, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_HYPNA (Q0C0N0)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760713.1
- ProteinModelPortal:   Q0C0N0
- SMR:   Q0C0N0
- STRING:   Q0C0N0
- GeneID:   4287822
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2014
- NMPDR:   fig|228405.5.peg.1940
- TIGR:   HNE_2014
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- PhylomeDB:   Q0C0N0
- ProtClustDB:   CLSK2531780
- BioCyc:   HNEP81032:HNE_2014-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 39494; Mature: 39363

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETVAIIVAGGRGQRAGAERPKQWQMLLGKRVIDWSIAAFVDHPQISQVVIVAGDELGD
CCCEEEEEEECCCCCCCCCCCCHHHHHHHCCHHHHEEHEEHCCCCCCCEEEEEECCCCCC
CSAEPKIIQAKPGNTRTQSVLSGLAAATISEDATVVIHDAARPGIDAATISSLIARLQDP
CCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC
SVSGAAPAMPVADALKTNSGQSWTNVDRTGLVRVQTPQAFRLGEIRAALSAAGPDLVDDL
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHH
TAIEAAGGRVEIVSGSARLTKITYPEDFDMLARLLSPTGAPRIGKGYDVHEFEAGDHVTL
HHHHCCCCEEEEEECCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEEE
CGVAIPHIAKLKGHSDADAAWHALTDAILGAVALGDIGDHFPPSDPQWKGADSGLFLKEA
ECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
QRLAEAKGYVIANCDITVICEAPKVKPHREAMRARTAELLGLPLDAVSVKATTTEGLGFT
HHHHHHCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
GRREGIAAEAVALLMPKG
CCCCCHHHHHHEEEECCC
>Mature Secondary Structure 
TETVAIIVAGGRGQRAGAERPKQWQMLLGKRVIDWSIAAFVDHPQISQVVIVAGDELGD
CCEEEEEEECCCCCCCCCCCCHHHHHHHCCHHHHEEHEEHCCCCCCCEEEEEECCCCCC
CSAEPKIIQAKPGNTRTQSVLSGLAAATISEDATVVIHDAARPGIDAATISSLIARLQDP
CCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCC
SVSGAAPAMPVADALKTNSGQSWTNVDRTGLVRVQTPQAFRLGEIRAALSAAGPDLVDDL
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHH
TAIEAAGGRVEIVSGSARLTKITYPEDFDMLARLLSPTGAPRIGKGYDVHEFEAGDHVTL
HHHHCCCCEEEEEECCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEEE
CGVAIPHIAKLKGHSDADAAWHALTDAILGAVALGDIGDHFPPSDPQWKGADSGLFLKEA
ECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
QRLAEAKGYVIANCDITVICEAPKVKPHREAMRARTAELLGLPLDAVSVKATTTEGLGFT
HHHHHHCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
GRREGIAAEAVALLMPKG
CCCCCHHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA