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The map label for this gene is cysK [H]

Identifier: 114799333

GI number: 114799333

Start: 41629

End: 42612

Strand: Direct

Name: cysK [H]

Synonym: HNE_0049

Alternate gene names: 114799333

Gene position: 41629-42612 (Clockwise)

Preceding gene: 114800485

Following gene: 114798524

Centisome position: 1.12

GC content: 63.21

Gene sequence:

>984_bases
ATGGCCGGAACAGTGAAACTCGAGGGCGGCGTAACGCTCGATGGACCGCGCGGCCGGATTTACGGCTCGATCCTTGAAAC
GGTTGGCGCGACCCCGCTCGTTCGCGCCGAAAAGTTCGCCAAGGCCGAGGGCGTTGACGCCGACATCCTCTTCAAGCTGG
AATTCTTCAATCCCCTCGCCAGCGTCAAGGACCGGATCGGCCTCGCGATGATCCTGGGACTGGAGGCCGAAGGCAAGCTG
AAGCCCGGCGGTGTCCTGATTGAGCCTACCTCGGGCAATACCGGCATTGGCCTCGCCTTCGTGGCGGCCGCCAAAGGCTA
CCGCCTGATCCTCACCATGCCGGAGTCCATGTCTGTGGAGCGGCGCAAGATGCTGGCCTATCTCGGCGCCGAGCTGGTGC
TGACGCCGAAAGAAAAAGGCATGGGCGGGGCCATCGCGCGGGCCAATGAACTGCTCGCCGAAATCCCTGGCGCCGTCAGC
CCCAGCCAGTTCGACAATCCGGCAAACCCGGCGATCCATGAGAAGACAACGGCAGAGGAAATCTGGTCCGATACCGCCGG
CGCTGTCGACGCCATTGTTTCGGGCATCGGCACGGGTGGCACGTTCACGGGCGTTGGGCGCGTGCTGAAGCCCCGCAAGC
CGAGCCTGAAGATGTTTGCGATTGAGCCTGAAGCCTCCCCGGTCTTGTCCGGCGGCGCACCTGGCCCCCATATGATCCAG
GGCATCGGCGCCGGCTTTGTGCCGGGTAACGCGGATACGAGCCTGATTGACGAGATCGTGAAAGTCTCCAATGAGGAATC
CTTTGCGATGGCCCGTAAGCTCGCCAAGACCGAAGGCATTCCCGGTGGTATTTCTTCCGGCGCGGCAGCCGCGGCAGCCG
TTCGCATCGGCAAGCGGCCGGAGATGGCGGGCAAGACAATCGTGGTCATCCTGGCAAGCTTTGCCGAGCGCTATCTCTCA
ACGGCTCTGTTTGACGGCGTCTGA

Upstream 100 bases:

>100_bases
GCTAGGCTGAGTTTAGCAGAGCTTACCCGAGTTTGGTTGCCATTCTCCCGCTGCGGGCCGATATTCTCCCTTCAAGATAA
TCCTGAAGGGAGAGTTCGGC

Downstream 100 bases:

>100_bases
CAGGGTAGGGGGCTTCGCAGCGGCAGGGCTCGCCAATCTTCGGCAGCTGGTGGATAGATGCCACCAGAAAATTCATCCGG
AGGAAGCCGATTATGGCGCG

Product: cysteine synthase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAGTVKLEGGVTLDGPRGRIYGSILETVGATPLVRAEKFAKAEGVDADILFKLEFFNPLASVKDRIGLAMILGLEAEGKL
KPGGVLIEPTSGNTGIGLAFVAAAKGYRLILTMPESMSVERRKMLAYLGAELVLTPKEKGMGGAIARANELLAEIPGAVS
PSQFDNPANPAIHEKTTAEEIWSDTAGAVDAIVSGIGTGGTFTGVGRVLKPRKPSLKMFAIEPEASPVLSGGAPGPHMIQ
GIGAGFVPGNADTSLIDEIVKVSNEESFAMARKLAKTEGIPGGISSGAAAAAAVRIGKRPEMAGKTIVVILASFAERYLS
TALFDGV

Sequences:

>Translated_327_residues
MAGTVKLEGGVTLDGPRGRIYGSILETVGATPLVRAEKFAKAEGVDADILFKLEFFNPLASVKDRIGLAMILGLEAEGKL
KPGGVLIEPTSGNTGIGLAFVAAAKGYRLILTMPESMSVERRKMLAYLGAELVLTPKEKGMGGAIARANELLAEIPGAVS
PSQFDNPANPAIHEKTTAEEIWSDTAGAVDAIVSGIGTGGTFTGVGRVLKPRKPSLKMFAIEPEASPVLSGGAPGPHMIQ
GIGAGFVPGNADTSLIDEIVKVSNEESFAMARKLAKTEGIPGGISSGAAAAAAVRIGKRPEMAGKTIVVILASFAERYLS
TALFDGV
>Mature_326_residues
AGTVKLEGGVTLDGPRGRIYGSILETVGATPLVRAEKFAKAEGVDADILFKLEFFNPLASVKDRIGLAMILGLEAEGKLK
PGGVLIEPTSGNTGIGLAFVAAAKGYRLILTMPESMSVERRKMLAYLGAELVLTPKEKGMGGAIARANELLAEIPGAVSP
SQFDNPANPAIHEKTTAEEIWSDTAGAVDAIVSGIGTGGTFTGVGRVLKPRKPSLKMFAIEPEASPVLSGGAPGPHMIQG
IGAGFVPGNADTSLIDEIVKVSNEESFAMARKLAKTEGIPGGISSGAAAAAAVRIGKRPEMAGKTIVVILASFAERYLST
ALFDGV

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=315, Percent_Identity=40.6349206349206, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI295821200, Length=315, Percent_Identity=40.6349206349206, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI4557415, Length=315, Percent_Identity=40.6349206349206, Blast_Score=225, Evalue=3e-59,
Organism=Escherichia coli, GI1788754, Length=307, Percent_Identity=54.3973941368078, Blast_Score=280, Evalue=8e-77,
Organism=Escherichia coli, GI2367138, Length=303, Percent_Identity=40.9240924092409, Blast_Score=214, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI115535073, Length=301, Percent_Identity=53.156146179402, Blast_Score=293, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI17535051, Length=300, Percent_Identity=51, Blast_Score=290, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI17562970, Length=303, Percent_Identity=49.1749174917492, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=49.8305084745763, Blast_Score=283, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI17561720, Length=300, Percent_Identity=49, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI32566672, Length=215, Percent_Identity=50.2325581395349, Blast_Score=218, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17534315, Length=324, Percent_Identity=41.6666666666667, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI25147552, Length=320, Percent_Identity=40.3125, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=50.8474576271186, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321594, Length=319, Percent_Identity=37.9310344827586, Blast_Score=189, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6321449, Length=318, Percent_Identity=32.0754716981132, Blast_Score=99, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24643623, Length=318, Percent_Identity=38.0503144654088, Blast_Score=198, Evalue=4e-51,
Organism=Drosophila melanogaster, GI20129101, Length=318, Percent_Identity=38.0503144654088, Blast_Score=198, Evalue=4e-51,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33668; Mature: 33537

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTVKLEGGVTLDGPRGRIYGSILETVGATPLVRAEKFAKAEGVDADILFKLEFFNPLA
CCCEEEECCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEHHHCHHH
SVKDRIGLAMILGLEAEGKLKPGGVLIEPTSGNTGIGLAFVAAAKGYRLILTMPESMSVE
HHHHHCCEEEEEEECCCCCCCCCCEEEECCCCCCCCHHHEEECCCCCEEEEECCCCCCHH
RRKMLAYLGAELVLTPKEKGMGGAIARANELLAEIPGAVSPSQFDNPANPAIHEKTTAEE
HHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
IWSDTAGAVDAIVSGIGTGGTFTGVGRVLKPRKPSLKMFAIEPEASPVLSGGAPGPHMIQ
HHCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHH
GIGAGFVPGNADTSLIDEIVKVSNEESFAMARKLAKTEGIPGGISSGAAAAAAVRIGKRP
HCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCC
EMAGKTIVVILASFAERYLSTALFDGV
CCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGTVKLEGGVTLDGPRGRIYGSILETVGATPLVRAEKFAKAEGVDADILFKLEFFNPLA
CCEEEECCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEHHHCHHH
SVKDRIGLAMILGLEAEGKLKPGGVLIEPTSGNTGIGLAFVAAAKGYRLILTMPESMSVE
HHHHHCCEEEEEEECCCCCCCCCCEEEECCCCCCCCHHHEEECCCCCEEEEECCCCCCHH
RRKMLAYLGAELVLTPKEKGMGGAIARANELLAEIPGAVSPSQFDNPANPAIHEKTTAEE
HHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
IWSDTAGAVDAIVSGIGTGGTFTGVGRVLKPRKPSLKMFAIEPEASPVLSGGAPGPHMIQ
HHCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHH
GIGAGFVPGNADTSLIDEIVKVSNEESFAMARKLAKTEGIPGGISSGAAAAAAVRIGKRP
HCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCC
EMAGKTIVVILASFAERYLSTALFDGV
CCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]