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The map label for this gene is nadB [H]

Identifier: 114800426

GI number: 114800426

Start: 700323

End: 701891

Strand: Reverse

Name: nadB [H]

Synonym: HNE_0690

Alternate gene names: 114800426

Gene position: 701891-700323 (Counterclockwise)

Preceding gene: 114799594

Following gene: 114797685

Centisome position: 18.94

GC content: 69.02

Gene sequence:

>1569_bases
ATGACGGCATTTCCTGATCGGATAATCACGATGCTTTCCGGAACGGATGACCGCCTGCATGCCGGCGCGGGCGCCACGAC
AGATGTTCTGATCGTCGGGGCGGGCCTTGCGGGCCTGTTCCTTGCGCTGAAGCTGGCGCCGCGGCCCTGCCTGGTGATCT
CACCGGCGCCGCTCGGGCAGGCGTCTTCCTCGGCCTGGGCGCAGGGGGGATTGGCCGCTGCGCTGCATCCGCTCGACAGC
CCGGCCCAGCATGCCGACGATACCGTGGCAGCAGGCGCCGGGCTGGTGGACCCGGTGATTGCGCGGCTGATCGCGGAAGA
CGGCCCCGCGCGCGTGCGCGACCTGGTGGCCCTCGGTGTACCGTTTGACCGGACGCCCGATGGCCAGCTCGCCCTGTCGC
TGGAGGCGGCACATTCGCATCCGCGTGTGGCGCGGGTTTCAGGCGACCTGGCGGGCAAGGCGATCATGGACGCGCTGGTG
GCGGCGGTGGCGGCAGCCCCGCATATCGAGCTGATCGAGGGCGCGCGGGCTGAGGCTCTGTTGCAGGATACCAATGGCCG
CGTGGCGGGGGTCGTGCTGCGCGGGAATACCGGGCGAGCGTTTGTGAAGACCGCGCGGGAGACAGTGCTGTGCACCGGTG
GGTCCGGTGGGCTTTACCGCGTGACGACCAATCCGAAGGAAGCCCAGGGCGAGGCGATTGCCATGGCGCATGTGGCGGGC
GCGGTGCTGGCCGACCTCGAATTCGTGCAGTTTCACCCGACCGGCATCGACATTGGCCGTGACCCTGCCCCGCTGGCGAC
CGAGGCGCTGCGCGGCGAAGGCGCGACGTTGCACAATACCGACGGGCGCGCCTTCATGGCAGATTATCATCCGCTGGCGG
AGCTTGCGCCGCGCGATGAAGTGGCCCGCGCGATCCATGCGGAGCGGGCCGCCGGGCGTGGCGCTTTTCTCAATTGCATC
ACGGCCGTGGGCGAAGAGTTTCCGCATCACTTCCCGACGGTTTTTGGCGCGTGCATGAGCGCCAACATCGATCCGCGCCG
CGCGATGATCCCGGTAGCGCCCGCCGCGCATTATCATATGGGCGGGATTATCTCTGACCTCTGGGGGCGCTCGACGCTGG
ATGGGCTCTCCGTCTGCGGAGAATGTTCCTCGACCGGCGCGCATGGGGCCAACCGGCTCGCCTCCAACTCTCTGCTGGAG
GCTGTCGTCTTTGCCGAGCGGATTGCGCGGCGCCTGCGCGACGCGGACCTCAACGAAGCGGGCGCGAGCACGGCATCCGC
AACGCGGCCCCTGACCGGCCCGGACATGCTGGAACTGCGTGAGGCGATGTCGCGCCATTGCGGCGTGGTGCGCGAGGCGG
GCGGGCTGACTGATCTTCTGAGCCTGATCGAGCGGCTGGAGGCAGGCATGCCCGGCGCGCCGCAGCTGACGGCTGCGCGC
CTGATCGCAAGCGGAGCGCTGGCCCGCGCCGAAAGCCGCGGCGGACATTTCCGGTCCGACTATCCCGACACCGGCGCGCC
CGAGCGCCAGTTCCTGTTCCACACAAGCCGCGAGGCGCTTGTCATATGA

Upstream 100 bases:

>100_bases
TATGTTCCTGACAATTTCACGCTGACGATCCAGTCCCACGAACGGATGTTCGGGGCGCTCGTCTCTGCTGTCACAGCGCC
GGTGGAATTCCGCCGCGCGA

Downstream 100 bases:

>100_bases
CGACCTATATTCCCCCGCTTTCCCCGATCATCCTTGATCCGATTGTCCGCCTGGCGCTGGCCGAGGATCTGGGCCGGGCG
GGCGACCTGACGACTGACGC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MTAFPDRIITMLSGTDDRLHAGAGATTDVLIVGAGLAGLFLALKLAPRPCLVISPAPLGQASSSAWAQGGLAAALHPLDS
PAQHADDTVAAGAGLVDPVIARLIAEDGPARVRDLVALGVPFDRTPDGQLALSLEAAHSHPRVARVSGDLAGKAIMDALV
AAVAAAPHIELIEGARAEALLQDTNGRVAGVVLRGNTGRAFVKTARETVLCTGGSGGLYRVTTNPKEAQGEAIAMAHVAG
AVLADLEFVQFHPTGIDIGRDPAPLATEALRGEGATLHNTDGRAFMADYHPLAELAPRDEVARAIHAERAAGRGAFLNCI
TAVGEEFPHHFPTVFGACMSANIDPRRAMIPVAPAAHYHMGGIISDLWGRSTLDGLSVCGECSSTGAHGANRLASNSLLE
AVVFAERIARRLRDADLNEAGASTASATRPLTGPDMLELREAMSRHCGVVREAGGLTDLLSLIERLEAGMPGAPQLTAAR
LIASGALARAESRGGHFRSDYPDTGAPERQFLFHTSREALVI

Sequences:

>Translated_522_residues
MTAFPDRIITMLSGTDDRLHAGAGATTDVLIVGAGLAGLFLALKLAPRPCLVISPAPLGQASSSAWAQGGLAAALHPLDS
PAQHADDTVAAGAGLVDPVIARLIAEDGPARVRDLVALGVPFDRTPDGQLALSLEAAHSHPRVARVSGDLAGKAIMDALV
AAVAAAPHIELIEGARAEALLQDTNGRVAGVVLRGNTGRAFVKTARETVLCTGGSGGLYRVTTNPKEAQGEAIAMAHVAG
AVLADLEFVQFHPTGIDIGRDPAPLATEALRGEGATLHNTDGRAFMADYHPLAELAPRDEVARAIHAERAAGRGAFLNCI
TAVGEEFPHHFPTVFGACMSANIDPRRAMIPVAPAAHYHMGGIISDLWGRSTLDGLSVCGECSSTGAHGANRLASNSLLE
AVVFAERIARRLRDADLNEAGASTASATRPLTGPDMLELREAMSRHCGVVREAGGLTDLLSLIERLEAGMPGAPQLTAAR
LIASGALARAESRGGHFRSDYPDTGAPERQFLFHTSREALVI
>Mature_521_residues
TAFPDRIITMLSGTDDRLHAGAGATTDVLIVGAGLAGLFLALKLAPRPCLVISPAPLGQASSSAWAQGGLAAALHPLDSP
AQHADDTVAAGAGLVDPVIARLIAEDGPARVRDLVALGVPFDRTPDGQLALSLEAAHSHPRVARVSGDLAGKAIMDALVA
AVAAAPHIELIEGARAEALLQDTNGRVAGVVLRGNTGRAFVKTARETVLCTGGSGGLYRVTTNPKEAQGEAIAMAHVAGA
VLADLEFVQFHPTGIDIGRDPAPLATEALRGEGATLHNTDGRAFMADYHPLAELAPRDEVARAIHAERAAGRGAFLNCIT
AVGEEFPHHFPTVFGACMSANIDPRRAMIPVAPAAHYHMGGIISDLWGRSTLDGLSVCGECSSTGAHGANRLASNSLLEA
VVFAERIARRLRDADLNEAGASTASATRPLTGPDMLELREAMSRHCGVVREAGGLTDLLSLIERLEAGMPGAPQLTAARL
IASGALARAESRGGHFRSDYPDTGAPERQFLFHTSREALVI

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=536, Percent_Identity=30.5970149253731, Blast_Score=162, Evalue=8e-40,
Organism=Escherichia coli, GI1788928, Length=517, Percent_Identity=37.330754352031, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI1790597, Length=523, Percent_Identity=31.5487571701721, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1786942, Length=541, Percent_Identity=30.8687615526802, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17505833, Length=543, Percent_Identity=28.1767955801105, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17550100, Length=512, Percent_Identity=29.1015625, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71986328, Length=423, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322701, Length=531, Percent_Identity=29.9435028248588, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6322416, Length=517, Percent_Identity=31.7214700193424, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6322511, Length=330, Percent_Identity=27.8787878787879, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6320788, Length=312, Percent_Identity=29.4871794871795, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17137288, Length=508, Percent_Identity=30.9055118110236, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24655642, Length=508, Percent_Identity=30.9055118110236, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24655647, Length=508, Percent_Identity=30.9055118110236, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24663005, Length=521, Percent_Identity=28.7907869481766, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 54099; Mature: 53968

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFPDRIITMLSGTDDRLHAGAGATTDVLIVGAGLAGLFLALKLAPRPCLVISPAPLGQ
CCCCHHHHHHHHCCCCCCEECCCCCCCEEEEECCCHHHHHHHHHCCCCCEEEECCCCCCC
ASSSAWAQGGLAAALHPLDSPAQHADDTVAAGAGLVDPVIARLIAEDGPARVRDLVALGV
CCCCHHHCCCHHHHHCCCCCCHHHCCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
PFDRTPDGQLALSLEAAHSHPRVARVSGDLAGKAIMDALVAAVAAAPHIELIEGARAEAL
CCCCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHH
LQDTNGRVAGVVLRGNTGRAFVKTARETVLCTGGSGGLYRVTTNPKEAQGEAIAMAHVAG
HHCCCCCEEEEEEECCCCHHHHHHCCCEEEEECCCCCEEEEECCCCHHCCCEEHHHHHHH
AVLADLEFVQFHPTGIDIGRDPAPLATEALRGEGATLHNTDGRAFMADYHPLAELAPRDE
HHHHCCEEEEEECCCCCCCCCCCCHHHHHHCCCCCEEECCCCCEEEECCCHHHHHCCHHH
VARAIHAERAAGRGAFLNCITAVGEEFPHHFPTVFGACMSANIDPRRAMIPVAPAAHYHM
HHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
GGIISDLWGRSTLDGLSVCGECSSTGAHGANRLASNSLLEAVVFAERIARRLRDADLNEA
HHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GASTASATRPLTGPDMLELREAMSRHCGVVREAGGLTDLLSLIERLEAGMPGAPQLTAAR
CCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHH
LIASGALARAESRGGHFRSDYPDTGAPERQFLFHTSREALVI
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEECC
>Mature Secondary Structure 
TAFPDRIITMLSGTDDRLHAGAGATTDVLIVGAGLAGLFLALKLAPRPCLVISPAPLGQ
CCCHHHHHHHHCCCCCCEECCCCCCCEEEEECCCHHHHHHHHHCCCCCEEEECCCCCCC
ASSSAWAQGGLAAALHPLDSPAQHADDTVAAGAGLVDPVIARLIAEDGPARVRDLVALGV
CCCCHHHCCCHHHHHCCCCCCHHHCCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
PFDRTPDGQLALSLEAAHSHPRVARVSGDLAGKAIMDALVAAVAAAPHIELIEGARAEAL
CCCCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHH
LQDTNGRVAGVVLRGNTGRAFVKTARETVLCTGGSGGLYRVTTNPKEAQGEAIAMAHVAG
HHCCCCCEEEEEEECCCCHHHHHHCCCEEEEECCCCCEEEEECCCCHHCCCEEHHHHHHH
AVLADLEFVQFHPTGIDIGRDPAPLATEALRGEGATLHNTDGRAFMADYHPLAELAPRDE
HHHHCCEEEEEECCCCCCCCCCCCHHHHHHCCCCCEEECCCCCEEEECCCHHHHHCCHHH
VARAIHAERAAGRGAFLNCITAVGEEFPHHFPTVFGACMSANIDPRRAMIPVAPAAHYHM
HHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
GGIISDLWGRSTLDGLSVCGECSSTGAHGANRLASNSLLEAVVFAERIARRLRDADLNEA
HHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GASTASATRPLTGPDMLELREAMSRHCGVVREAGGLTDLLSLIERLEAGMPGAPQLTAAR
CCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHH
LIASGALARAESRGGHFRSDYPDTGAPERQFLFHTSREALVI
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]